Objective To study the association of SNP of the AdipoR1 gene with T2DM in Xi’an population. Methods The amplification refractory mutation system(ARMS) analysis and gene sequencing were used to investigate the AdipoR1 gene polymorphism in 100 type 2 diabetics and 84 normal control subjects. Results (1) The genotype and allele frequencies of -106A/G, 5843A/G were not significantly different between type 2 diabetics and normal control subjects. (2) The diagnosis age of diabetics was significantly younger in AdipoR1 5843GG genotype group than in other genotype groups. Conclusions The data implicate that the AdipoR1 gene -106A/G and 5843A/G polymorphism may be not associated with pathogenesis of T2DM. AdipoR1 5843 GG genotype may be associated with the earlier diagnosis of T2DM

Objective To investigate the causes and therapy of complications of microvascular decompression (MVD) for hemifacial spasm (HFS). Method Totally 255 cases of HFS underwent MVD in our hospital from December 2000 to March 2000. Among the cases,207 patients were followed up for more than 1 year,the occurrence and recovery of their postoperative complications were reviewed in this study. Results In this series,no patient died;cerebrospinal fluid leakage was detected in 15 patients (7.2%) in one week after the operation;facial palsy occurred in 48 cases (23.2%);hearing deficit was shown in 20 patients (9.7%);and tinnitus was found in 12 cases (5.8%). The patients were followed up for 1 to 7.3 years with a mean of (4.2?2.7) years,during the period,none of the cases had cerebrospinal fluid leakage or intracerebral infection,and the cases who had facial palsy and tinnitus recovered completely;only 10 patients remained hearing deficit (including unilateral deaf in 6) during the follow-up.Conclusion Cerebrospinal fluid leakage,facial palsy,hearing deficit,tinnitus are the major complications of MVD,among which cerebrospinal fluid leakage,facial palsy,and tinnitus can be improved after treatments,but hearing deficit is non-reversible.

Child ; Hemorrhagic Fever with Renal Syndrome ; Humans

Objective To study the changes of diamine oxidase (DAO )and intestinal fatty acid binding protein (I-FABP)levels in neonates with hypoxic-ischemic encephalopathy treated with selective brain hypothermia.Methods Collect a sample of 60newborns with moderate and severe hypoxic-ischemic encephalopathy who were hospitalized in the NICU of Matemal and Child Health Care Hospital of Baoding from June 2013to December 2014.The 60newborns were divided into two groups randomly:hypothermia group(n=30)and conventional treatment group(n=30).Selected 30cases hospitalized at the same period, except the related to the ischemia hypoxia and gastrointestinal dysfunction disease as the control group.The levels of serum levels of DAO and I-FABP were measured by ELISA on admission and 7days after treat-ment,respectively.And the score of gastrointestinal dysfunction were compared.Results Neither the levels of DAO and I-FABP in hypothermia group and conventional treatment group had statistical differences on ad-mission[DAO:(15.77±2.04)U/ml,(15.81±1.85)U/ml,P﹥0.05;I-FABP:(310.01±46.43)ng/L, (301.12±38.61)ng/L,P﹥0.05],but were higher than that in the control group [(7.65±0.74)U/ml, (51.65±6.91)ng/L].Seven days after treatment,both the levels of DAO and I-FABP of hypothermia group and conventional treatment group decreased [DAO:(7.88±1.87)U/ml,(12.51±1.53)U/ml;I-FABP:(59.16±6.17)ng/L,(121.31±21.54)ng/L],meanwhile,the variation of hypothermia group was more significant(P﹤0.05).The correlation of the plasma DAO and I-FABP levels and the score of gas-trointestinal dysfunction was significantly (r1=0.831,r2=0.827,P ﹤0.01).Conclusion Hypothermia treatment could effectively reduce the levels of DAO and I-FABP,thus improve the gastrointestinal function in some extent.

Objective To assess the effectiveness of continuous aspiration of subglottic secretions (CASS)in preventing ventilator-associated pneumonia.Methods The relevant randomized controlled trials were searched in the Cochrane Library,Pubmed,CNKI,VIP and Wanfang databases.The experimental group received CASS and the control group did not(NASS group).Two reviewers selected studies according to the inclusion and exclusion criteria,the quality of studies was critically appraised and data were extracted by two reviewers independently.Meta-analysis was conducted by using RevMan 5.2.Results Sixteen randomized controlled trials met the inclusion criteria and enrolled 2 576 patients.According to the meta analysis,compared with NASS,CASS reduced the incidence of ventilator-associated pneumonia,shortened the length of stay in the intensive care unit and the duration of mechanical ventilation.There was no effect on hospital mortality.Conclusions Continuous aspiration of subglottic secretions appears effective in preventing ventilator-associated pneumonia,decreasing the duration of intensive care unit length of stay and mechanical ventilation among critically ill patients with mechanical ventilation.

Objective To analyze the causes and preventions of the perioperative complications of micro-laparoscopic hernioplasty in infants and children with indirect inguinal hernia.Methods From January 1997 to January 2007,4500 infants or children with indirect inguinal hernia,including 702 cases who were complicated with hydrocele testis,were treated by micro-laparoscopic hernioplasty in our hospital.By laparoscopy,the endocycle was sutured circularly under the peritoneum using a self-designed needle.Then,only the peritoneum was ligated.Results No organ injury or complication occurred during the operation.The mean operation time was 9 min(5-13 min).Among the patients,4250 received a follow-up of 7-36 months(mean,25 months).Postoperative complications were found in 132 patients(3.1%),included scrotal gas in 10(0.24%),scrotal hydrocele in 16(0.38%),umbilical hernia in 27(0.64%),extraperitoneal emphysema in 20(0.47%),abdominal wall hematoma at the burr hole in 15(0.35%),foreign body sensation at the suture sites in 30(0.71%),scrotal hematoma in 8(0.19%),and iatrogenic cryptorchism in 6(0.14%).Thirty-five patients had recurrence(0.82%).Conclusions Micro-laparoscopic hernioplasty is a safe and reliable method for indirect inguinal hernia in infants and children.It is important to be familiar with the anatomy of the hernia under a laparoscope and to perform the operation carefully so that to avoid or decrease the perioperative complications.

0.05 ), and the effect in Group A and Group B was superior to Group C(P

[Objective] To investigate the therapeutic effect of early anterior bone grafting and internal fixation after focal cleaning and vertebral canal decompression for the treatment of tuberculosis of thoracolumbar vertebrae. [ Methods ] A retrospective study was carried out in 26 patients with thoracolumbar vertebral tuberculosis (TVT) who received early anterior bone grafting and internal fixation after focal cleaning and vertebral canal decompression. After treatment, the therapeutic effect was evaluated by observing the recovery of nerve function. [Results] The result of a follow-up ranging from 8 months to five years and 4 months showed that all the cases wi vertebral tuberculosis were cured, free from relapse. The internal fixation and bone graf(?)ing for fusion were good, and nerve function recovered, maintaining a good state. The nerve function of six patients with incomplete paralysis fully recovered. [Conclusion] Early anterior bone grafting and internal fixation after focal cleaning and vertebral canal decompression are effective to promote the recovery of nerve function of TVT patients. This regimen is capable of relieving or preventing spinal compression promptly, rebuilding the stability of the spine and presenting the focal diffusion, beneficial to the recovery of the patients.

[Objective] To investigate the effect of SKY expander vertebroplasty and KYPHON vertebroplasty for compression fracture of thoracic/lumbar vertebral body. [Methods] Twenty-one patients (group A) with compression fracture in 27 thoracic/lumbar vertebral bodies were enrolled to this study. Among them, 17 fractured vertebral bodies from 15 patients received SKY expander vertebroplasty and another 10 fractured vertebral bodies from 6 patients received KYPHON vertebroplasty. Thirty-three patients (group B) with compression fracture in 35 thoracic/lumbar vertebral bodies receiving conservative treatment during the same period served as the control. After treatment, the therapeutic effect and result of X-ray image were compared in the two groups. [Results] A follow-up ranging from 1 month to 11 months showed that pain in the loin and back, decrease of vertebral height, complications of leaking of bone cement, and bed sore (which occurred in group B) were not found in the patients receiving vertebroplasty. [Conclusion] SKY expander vertebroplasty and KYPHON vertebroplasty are effective and safe for compression fracture of thoracic/lumbar vertebral body, which can relieve the back and loin pain as soon as possible, reconstruct the vertebral body, decrease the occurrence of complications and promote the rehabilitation, and have a better effect than conservative treatment. For the two kinds of vertebroplasty, SKY expander vertebroplasty is economic and indicated for single vertebral fracture and old fracture, and KYPHON vertebroplasty is indicated for multiple vertebral fracture, severe fracture, and vertebral body fracture with end-plate broken.

OBJECTIVE This study is to investigate the effects on nasopharyngeal carcinoma cell lines(CNE-2Z)induced by As2O3 and its possible mechanisms.METHODS CNE-2Z cells were treated with various concentrations of As2O3 for different times.The IC50 values were detected by trypan blue stain assay to choose the optimal concentration of As2O3.Cell cycle redistribution was analyzed by flow cytometry.The expression levels of p16、 RASSF1A、DNMT 3B mRNA were assessed by RT-PCR.RESULTS The suppression of cell proliferation by As2O3 was time and dose-dependent.After being treated with different concentration of As2O3,the IC50 values of As2O3 were(1.50?0.05),(1.09?0.13),(0.65?0.04)?mol/L at 24,48,and 72h,respectively.As2O3 also arrested CNE-2Z cells in G2/M phase of cell cycle.After CNE-2Z cells being treated with As2O3,the expression of RASSF1A mRNA were up-regulated(P