This article discusses the experience of professor SUN Wei-feng in diagnozing and treating PCOS(polycystic ovary syndrome) from four aspects:the cuase of the disease,the thought of treatmeat,the differentiation of syndrom,and the experience of treatment,combination of prescriptions.Professor SUN thinks that its pathology basis is deficiency of kidney and blood stasis,and points out that the method of tonifying kidney and promoting blood could be considered as the basic way of treat PCOS in TCM,the treatment cycle of the TCM is the important links to this disease,it should according with the different syndroms to use different methods on clinic.

AIM: To observe the pathological role of 3-nitrotynosine (3-NT) on Escherichia coli LPS-induced vascular hyporeactivity in rats and the therapeutic effect of antioxidants. METHODS: Forty male SD rats weighting from 200 g to 250 g were randomly divided into four groups: the control group (n=10); LPS shock group (n=10); uric acid-treated group (n=10); melatonin-treated group (n=10). 6 h after LPS shock, phenylephrine (0.5-2.5 ?g?kg -1) was applied intravenously to all groups and the percentage increase in MAP was detected, respectively. The concentration-response curve of aorta rings from all groups rats were obtained by cumulative addition of phenylephrine (PE), and PE E_ max, EC_ 50 were calculated. The concentrations of plasma malondialdehyde (MDA), nitrate/nitrite and 3-NT were assayed in all groups 6 h after LPS shock. RESULTS: The MAP level induced by PE significantly decreased to 54.60% in LPS shock rats compared with the control (P

China ; Humans ; Root Canal Therapy

OBJECTIVETo observe the lower gastrointestinal mesenchymal tumors CT virtual endoscopy and pathology, and to explore its possible mechanism.

METHODSSelect 80 patients suspected colon gastroenterology treat in the hospital gastroenterology department, randomly divided into experimental group and control group, 40 cases in experimental group were given CT virtual endoscopy and pathological check, the control group received conventional colonoscopy and pathological check, and all the results compared with the pathological results.

RESULTSCompared with the pathological test results, consistent rate with the experimental group reached 94.73%, the same rate was 92.85% in control group. The difference was not statistically significant (P > 0.05).

CONCLUSIONSCT virtual endoscopy can quickly image and draw detailed information, and can improve the sensitivity and specificity of cancer diagnosis greatly. Clinically, CT virtual endoscopy have the same function as colonoscopy, can replace colonoscopy, can be important complement of electronic colonoscopy by those who cannot tolerate electronic colonoscopy or have contraindications. Its value is worth taking a step forward in-depth study.

Adult ; Aged ; Colonoscopy ; Colorectal Neoplasms ; diagnostic imaging ; pathology ; Endoscopy, Gastrointestinal ; methods ; Female ; Humans ; Male ; Mesenchymoma ; diagnostic imaging ; pathology ; Middle Aged ; Tomography, X-Ray Computed

Objective To explore the curative effects of color Doppter ultrasound guided interventional therapy in pelvic cyst. Methods From July 2006 to June 2008,the color Doppler ultrasound guided transabdominal or transvaginal puncture was performed in 34 pelvic cysts among 32 diagnosed cases,absolute alcohol was injected into the cysts for sclerotherapy.Results Among 34 cysts,the treatment only failed in one cyst of one case because of the over ropy hydatid fluid,which was hard to be aspirated.All of the cases were followed up for three months.Twenty-seven cysts were cured after the first treatment,the cure rate was 79.4%(27/34).The maximum diameter of other 4 cysts decreased more than 1/2.The effective rate was 91.2%(31/34) after the first puncture.Finally,33 cases showed curative effect after the puncture therapy,the total effective rate was 97.1%(33/34).No serious complications were observed.Conclusion This therapy is a simple,accurately,safe and effective treatment for many indications.It's worthy to be recommended in the therapy of pelvic cyst.

Objective]The article summarizes the experience of professor Zhou Enchao in treating diabetic nephropathy . [Methods]Pro.Zhou Enchao treats diabetic nephropathy by pairing drugs; discuses the clinical experience of professor Zhou Enchao in the treatment on diabetic nephropathy from the pathogenesis,treatment,and one case. [Results]Professor Zhou Enchao believes that the disease is caused by Qiyin deficiency, mixed with wet ,Fengxie and blood stasis. Pro.Zhou Enchao treats diabetic nephropathy by pairing drugs,such as astragalus membranaceus and Chinese yam,radix rehmanniae recene and radix scrophulariae,corn stigma and loofah sponge,stiff silkworm and scorpio,salvia miltiorrhiza and earthworm. And these usually lead to good curative effect. [Conclusion]Professor Zhou's clinical experience in treating diabetic nephropathy from pairing drugs is well worth extending in clinic for it always has significant effect.

Focal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase (PTK) that can localize indirectly to sites of clustering integrin family of heterodimeric receptors. As an important structure and signaling molecule in the adhesive complexes, which are large and stable referred as ‘focal adhesions’ or relatively small and transient within filopodia and lamellipodia named ‘focal complexes’, FAK is closely related with cell death, proliferation and migration. In this review, we discuss the function of FAK in the regulation of endothelial cell migration based on current data.

With an increasing prevalence all over the world,diabetes mellitus is considered as a potential cause of liver cancer in patients with non-viral hepatitis.Whether diabetes mellitus is the cause of liver cancer and related pathogenesis remain unknown.The article reviews recent large-sample cohort studies and confirms that diabetes mellitus increases the incidence rate of liver cancer and affects its prognosis.This article also investigates the association of hepatitis C,obesity,and nonalcoholic fatty liver disease with diabetes mellitus and liver cancer and finds that insulin resistance and activation of chronic inflammatory factors may be involved in the generation and proliferation of cancer cells.This article elaborates on the influence of anti-insulin resistance drugs on the development and progression of liver cancer and points out that diabetes mellitus may be the cause of liver cancer.Effective control of insulin resistance can help to reduce the development and progression of diabetes-associated liver cancer.

Objective To investigate the effects of sevoflurane on the systemic inflammatory response and cardiopulmonary function in septic shock rats. Methods Thirty-two SD rats, 8-10 months old, weighing 250-300 g, were randomly divided into 4 groups (n = 8 each): sham operation group (group S), cecal ligation and puncture (CLP) induced septic shock group (group CLP) , sevoflurane I group (group SEV, ) and sevoflurane II group (group SEV,). The abdomen was opened but CLP was not performed in group S. The septic shock was induced by CLP as described by Baker et al. Group SEV, and SEV, inhaled 2.4% sevoflurane for 30 min at 1 h and 3 h after the successful establishment of the model respectively. At 1, 3 and 5 h after septic shock, MAP and HR were recorded and arterial blood samples were taken for blood gas analysis and determination of plasma concentrations of TNF-α, IL-1, MDA and NO. The left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), left ventricular fractional shortening (LVFS) and cardiac output (CO) were also detected 5 h after septic shock. The animals were killed after the detection of cardiac function. The lungs were removed for determination of W/D lung weight ratio and Evans blue (EB) content. The tissues from the heart, lung, liver and kidney were taken for detection of NF-kB activity by electrophoretic mobility shift assay (EMSA) ResultsMAP was significantly lower, HR higher, LVEDD, LVESD, LVFS, CO, pH value, PaO2 and PaCO2 lower, and W/D lung weight ratio, EB content, plasma concentrations of TNF-α, IL-1, MDA and NO, and NF-kB activity in the heart, lung, liver and kidney tissues higher in group CLP, SEV, and SEV2 than in group S (P < 0.05). NF-kB activity in the heart, lung, liver and kidney tissues and plasma concentrations of TNF-α, IL-1, MDA and NO were significantly lower in group SEV, than in group CLP and SEV2 ( P < 0.05 ), but no significant differences were found in the other indices between group SEV, and CLP and between group SEV1 and SEV2 ( P > 0.05). Conclusion Inhalation of 2.4% sevoflurane for 30 min 1 h after septic shock can inhibit the systemic inflammatory response slightly, but can not improve the cardiopulmonary function in rats with CLP-induced septic shock.

Objective To investigate the effects of macmphage migration inhibitory factor (MIF) on glucocorticoid (GC) re]ease and glucocorticoid recer (GR) in mts.Methods Test Ⅰ Thirty-two male SD rats weighing 250-300 g were randomly divided into 4 groups(n=8 each):control group(C),low dose recombinant MIF (rMIF) group (rMIF-L),middle dose rMIF group (rMIF-M) and high dose rMIF group (rMIF-H).The animals received l ml normal saline via the right femoral vein in group C.The animals received rMIF50.100 and 200 ng in l ml normal saline though right femoral vein in group rMIF-L,rMIF-M or rMIF-H respectively.Blood samples were taken from left femoral artery immediately before inection(T0,baseline),and at 5 min,3 h,6 h.12 h and 24 h after injection of rMIF(T1-5) for determination of serum concentration of corticosterone.Test Ⅱ Primary cultured neonate rat(2-3 days)myocardial ceils were randomly divided into 3 groups(n=24 each):group C,group rMIF-L and group rMIF-M.The ceUs in group C,rMIF-L and rMIF-M wefe incubated with DMEM.rMIF 50 ng+DMEM and rMIF 100 ng+DMEM for 3 h respectively.The expression of GR and HsPg0 wag determined by Western blot.ResuBs Test Ⅰ The serum concentration of corticosterone was signifieemily higher in the other 3 groups than in group C at T1-5(P<0.05).The sertlm concentration of corticostemne was significantly increased at T1-5 in group rMIF-L,rMIF-M and rMIF-H compared with the baseline values(P<0.05).Test Ⅱ HSP90 expresion was significantly lower in the other two groups than in group C(P<0.05).Them was rio signifieanf difference in HSP90 expression between group rMIF-L and group rMIF-M(P>0.05).There was no significant difference in GR expression among the 3 groups ( P > 0.05). Conclusion MIF druing sepsis can weaken GR function through down-regulating HSP9O expression, resulting in CC resistance.