Objective:This study aims to explore the relationship between serum response factor (SRF) expression level and gas-tric cancer progression by detecting SRF expression level in cancer cells. Methods:The SRF gene in SGC-7901 cells was silenced by RNA interference. Transfection efficiency was detected by fluorescence microscopy, cell proliferation by CCK 8 method, SRF gene and protein expression level by real-time polymerase chain reaction and Western blot, and cell cycle by flow cytometry. Results:Cell treat-ment with siRNA-SRF induced significant reduction in SRF mRNA levels. Western blot analysis showed that SRF protein decreased by 40.1%in the siRNA group compared with that in the control group (P<0.05). Compared with the blank, negative, and mock transfection control groups, cell proliferation of the siRNA-SRF group decreased. The inhibition ratio reached 64.24%, as measured by the CCK-8 assay (P<0.05). Treatment with siRNA could block SGC-7901 cell cycle at G0/G1 phase (P<0.05). Conclusion:SRF expression is close-ly associated with gastric carcinoma cell proliferation. SRF protein level detection can provide a certain reference value in evaluating malignant gastric carcinoma progression. SRF is possibly an important target for the prevention and control of gastric cancers.

Objective To investigate the expressions of EphA2 and its ligand EphrinA1 and their relationship with angiogenesis in gastric carcinoma. Methods Expressions of EphA2,EphrinA1 and CD34-stained microvessel density (MVD) were detected by immunohistochemical assay in gastric carcinoma tissues,adjacent tissues (1.5 to 2 cm from the mass) and normal gastric mucosa (5 to 10 cm from the mass) from 82 cases. The correlations among EphA2 and EphrinA1 expressions,MVD and clinic pathological features were analyzed. Results EphA2,EphrinA1 expressions and MVD in gastric carcinomas tissues were significantly higher than those in adjacent tissues and normal gastric tissues (P

Aim To investigate the effect and mechanism of valdecoxib on the apoptosis of human esophageal cancer cells.Methods Flow cytometry was used to observe the effect of valdecoxib on apoptosis and the cell cycle distribution of Eca109 cells.Transmission electron microscope was further used to detect the cell apoptosis.The content of LDH was examined using LDH kit.The expressions of p-p38MAPK,Fas and FasL protein were detected using flow cytometry.Results Valdecoxib of 25～400 ?mol?L~(-1) significantly induced the apoptosis of Eca109 cell line,and the rate of apoptosis was increased from(2.95?0.83)% to(48.46?0.73)%,50～400 ?mol?L~(-1) valdecoxib also decreased the proliferation index and the proportion of cells in the S phase,increased the proportion of cells in the G_0/G_1 phase,but had no effect on the proportion of cells in the G_2/M phase.Compared with those in Eca109 cells cultured in the medium with solvent,the expression of p-p38MAPK,Fas and FasL was higher in the Eca109 cells exposed to valdecoxib in a dose-dependent manner in 72 h.Conclusion Valdecoxib can induce apoptosis of Eca109 cell line partly by up-regulating the expression of p-p38MAPK/Fas/FasL.

Objective To investigate the effects of the gene expressions of FAS、FASL、FADD and caspase-8 in esophageal carsinogenesis. Methods Immunohistochemical method was applied to detect the expression of FAS、FASL、FADD and caspase-8 proteins in esophageal epithelium. In situ hybridization method was applied to detect the expression of FADD and caspase-8 mRNA in esophageal epithelium. Results The positive rates of FAS, FADD and caspase-8 proteins and FADD、caspase-8 mRNA were decreased from normal epithelium to dysplasia and carcinoma tissues gradually. The positive rates of FASL protein were increased from normal epithelium to dysplasia and carcinoma tissues gradually. There was very significant statistical difference between carcinoma and normal epithelium(P

Objective To study the relationship between osteopontin (OPN) gene expression and the development and metastasis of esophageal cancer, and explore the effect of growth inhibitory of artesunate (Art) on regulating the expression of OPN in esophageal carcinoma cells.Methods The expressions of OPN gene and protein were detected in normal esophageal tissue (24 cases), esophageal dysplasia (21 cases) and esophageal squamous cell carcinoma (ESCC) tissue (45 cases) byin situ hybridization and immunohistochemistry, and the relationships between the OPN gene and protein expressions and the pathological features were analyzed with SAS software. After intervention with different concentrations of Art (0, 30, 60, 120mol/L) on ESCC Eca109 cells for 24h, the levels of OPN expression and cell cycle were detected by flow cytometry.Results The expression levels of OPN gene and protein were significantly higher in esophageal cancer tissues than in esophageal dysplasia and normal esophageal tissue (P<0.01), and in esophageal dysplasia than in normal esophageal tissue (P<0.05). The expression levels of OPN gene and protein in esophageal cancer tissues showed no relations with patients' gender, age and the degree of tumor differentiation (P>0.05), but showed a positive correlation with lymph node metastasis and the invasion depth of esophageal cancer (P<0.05). After treatment by Art for 24h, the proliferation index of Eca109 cells and the expression of OPN protein decreased significantly (P<0.01) in a concentration dependent manner.Conclusions The abnormal high expression of OPN gene may participate in the occurrence, invasion and metastasis of esophageal cancer, so can be used as an objective index of metastasis for esophageal cancer. Art inhibits the growth of esophageal carcinoma cells by down-regulating the expression of OPN protein.

Nuclear DNA content in 103 cases of malignant and benign breast tumours were determined by means of flow cytometry. It was found that DNA in all the benign lesions showed diploids. In contrast, only 32.3 percent of the cancers had diploids, while 62.3% had additional DNA aneuploids including near-diploids (18.4%); triploids (22.3%); and tetraploids (8.2%) etc. Both the cellular proportion in S-phase and DNA index were significantly higher in malignant breast tumours than those in benign ones (P0.05). The results indicate that the analysis of DNA content is a useful and objective adjunct in the assessment of proliferative activity and biological behavior on breast tumour.

Cell DNA content in heart, liver, kidney of rats were analysed by flow cytometer at different postmortem interved. The rsults show that mean cellular DNA content is 99.5% at 6 hours, 91.3% at 12 hours, 87.1% at 18 hours, 81.3% at 24 hours, 76.7% at 30 hours, 74.3% at 36 hours, 72.3% 48 hours as compared with that at o hours The results ingicates that the quantitative determination of cell DNA in the tissues, mentioned above may provide an objective and reliable approach for the estimation of postmortem time.

Objective To explore the expression of ATP-binding cassette transporter C2(ABCG2) in adriamycin(ADM)-resistant human esophageal cancer cells.Methods The ADM-resistant human esophageal cancer cell(Eca109/ADM) was induced by gradually increasing the ADM concentration in the culture medium of human esophageal cancer cell line(Eca109) and long time screening culture.ABCG2 mRNA and protein of ADM-resistant cells was detected by RT-PCR,flow cytometry(FCM) and Western blot.Drug excretion of Eca109/ADM cells was examined by FCM.The drug resistance index to ADM was detected by MTT.Results The expression of ABCG2 in Eca109/ADM cells was higher than that in Eca109 cells.The drug excretion of Eca109/ADM cells was stronger than Eca109 cells.The Ecal09/ADM cells drug resistance index to ADM was 3.29.Conclusion The ADM-resistant cell line Eca109/ADM was established successfully as an ideal chemoresistant cell model.ABCG2 might be involved in the drug resistance of esophageal cancer cell.

Objective To investigate the effect and possible mechanism of high mobility group box (HMGB) 1 in the development and progress of rheumatoid arthritis.Methods PBMC and serum samples were obtained from 74 RA patients (38 in active stage and 36 in stable stage) and 26 healthy controls.The expression of HMGB1 mRNA and protein was detected by RT-PCR and ELISA.Flow cytometry analysis ( FCM ) was used to detect the expression of Toll-like receptor 4 on PBMC.Results ①The expression of HMGBI mRNA and protein in active RA patients was significantly higher than that in healthy controls and inactive RA patients [2.63 vs 0.71，0.93 and (10.2±1.2) vs (7.5±1.8)，(8.3±1.8) ng/ml，respectively](P＜0.01 ).② The relative expression of TLR4 protein on CD14+ monocytes and CD3+ lymphocytes in active RA patients was increased than that in inactive RA and healthy controls (P＜0.05 or P＜0.01 ).It was also higher in inactive RA than in healthy controls (P＜0.05 or P＜0.01 ).③ Level of HMGB1 protein in serum of RA patients was positively correlated with ESR，CRP，RF，the numbers of tender joints and swollen joints as well as radiographic changes.Conclusion HMGB1 can be synthesized and released by PBMC of active RA patients，and then bind to TLR4 of PBMC to promote inflammatory responses and bone erosion.