BACKGROUND:Studies has confirmed that platelet-rich plasma(PRP) can affect the proliferation and differentiation of bone marrow mesenchymal stem cells and adipose derived stem cells. OBJECTIVE:To observe the effects of PRP on the proliferative and osteogenetic activity of skeletal muscle stem cells(SMSCs) in vitro,in addition,to elucidate the potential mechanism by which PRP affects SMSCs. DESIGN,TIME AND SETTING:A randomized controlled animal experiments was completed in the Center Laboratory of the Sixth People's Hospital Affiliated to Shanghai Jiao Tong University from June to October in 2008. MATERIALS:Nine New Zealand,ordinary grade rabbits,weight of 2.5-3.0 kg,1 year old,irrespective of gender. METHODS:The right soleus was dissected and cultured for SMSCs. PRP was prepared with central artery of rabbits’ ears. The cells were randomly divided into the experimental group and the control group. In the experimental group,the SMSCs were interfused with PRP(special culture media including 12.5% PRP) . There was no intervention in the control group. MAIN OUTCOME MEASURES:①Morphological observation. ②The proliferative ability of the cells was detected with MTT. ③ The osteogenetic ability was measured with alkaline phosphatase(AKP) staining,alizarin red staining and osteocalcin immunofluorescence assay. RESULTS:MTT method showed that the proliferative activity of the experimental group was obviously stronger than that of the control group(P

BACKGROUND:Hip fracture has become one of the main reasons for morbidity, death and heavy medical costs in the elderly. With aging, the incidence of hip fractures in the elderly is increased day by day. OBJECTIVE:To investigate and analyze the incidence and distribution of hip fractures in middle-aged and old population from Changning District of Shanghai, China. METHODS:Medical records of patients with hip fracture ICD-9 CODE (femoral neck fracture and intertrochanteric fracture) admitted from July 2008 to June 2013 were col ected at the Changning District Public Health Information Center and Shanghai Shen Kang Medical Al iance Center. Population distributions of Changning District in 2010 and 2011 were provided by the Disease Control Center of Changning District. Then, the crude morbidity of hip fracture was calculated in terms of different ages and genders. For patients aged over 50 years, every 5 years served as an age group. RESULTS AND CONCLUSION:The incidence rate of hip fractures in the population aged over 50 years was 197.04/100 000, 88.51/100 000 in male and 304.73/100 000 in female. The annual standardized incidence rate of hip fracture was higher in female than male (P<0.05). The crude incidence rate of hip fracture was increased dramatical y in the population over 70 years, especial y in females. It indicates that the incidence rate of hip fractures is closed related to age, gender and osteoporosis.

OBJECTIVETo investigate the clinical efficacy of ligation of intersphincteric fistula tract (LIFT) in the treatment of simple anal fistula, including transphincteric anal fistula and insphincteric anal fistula.

METHODSClinical data of 52 patients with anal fistula receiving surgery treatment in Beijing Anorectal Hospital from January to October 2014 were analyzed retrospectively. Adoption of surgical procedure was based on rectal endoluminal ultrasound and patients' decision. Patients were divided into LIFT group and seton group. The two groups were compared in terms of operation time, blood loss, postoperative pain score, incidence of urinary retention, wound healing time, cure rate, recurrence, and the anal incontinence score.

RESULTSThere were 52 patients in the entire cohort including 28 cases of transphincteric anal fistula (14 cases of LIFT and seton placement groups) and 24 cases of intersphincteric anal fistula (12 case of LIFT and seton placement). The operation time was shorter in seton placement group in patients with two simple anal fistula [(23.9±5.0) min vs. (46.3±7.7) min, P<0.05]. LIFT postoperative pain score [(1.6±0.6) vs. (6.1±1.3)], wound healing time [(7.9±2.0) days vs. (30.0±5.1) days], postoperative hospital stay [(10.3±3.1) days vs. (20.7±7.1) days], and anal incontinence scores [(1.1±0.4) vs. (4.9±1.1)] were better than that of anal fistula seton (all P<0.05). There was no statistically significant difference in intraoperatie blood loss [(23.1±4.7) ml vs. (23.3±4.7) ml, P>0.05]. The cure rate of intersphincteric anal fistula was 83.3%(10/12) in LIFT group, and 100%(12/12) in the seton group. The cure rate of transphincteric anal fistula was 78.6% (11/14) in LIFT and 92.9%(13/14) in anal fistula seton group. There was no statistically significant difference (P>0.05).

CONCLUSIONIn the treatment of transphincteric fistula tract and intersphincteric fistula tract, LIFT procedures should be considered.

Digestive System Surgical Procedures ; Fecal Incontinence ; Humans ; Length of Stay ; Ligation ; Operative Time ; Pain, Postoperative ; Postoperative Period ; Rectal Fistula ; Recurrence ; Retrospective Studies ; Wound Healing