Actins ; metabolism ; Adult ; Breast Diseases ; metabolism ; pathology ; surgery ; Breast Neoplasms ; pathology ; Calcium-Binding Proteins ; metabolism ; Carcinoma ; pathology ; Diagnosis, Differential ; Fasciitis ; metabolism ; pathology ; surgery ; Female ; Fibroma ; pathology ; Fibrosarcoma ; pathology ; Follow-Up Studies ; Humans ; Microfilament Proteins ; metabolism ; Vimentin ; metabolism ; Young Adult

Adenocarcinoma ; diagnosis ; pathology ; Carcinoma, Squamous Cell ; diagnosis ; pathology ; Cytodiagnosis ; methods ; Cytological Techniques ; methods ; Female ; Humans ; Lung Neoplasms ; diagnosis ; pathology ; Male ; Middle Aged ; Small Cell Lung Carcinoma ; diagnosis ; pathology ; Sputum

Objective:To observe the pathological changes of VX2 implanted breast cancer ablated by high intensity focused ultrasound (HIFU) in rabbits. Methods:Thirty-six rabbits implanted VX2 breast carcinoma were randomly divided into treatment group (n=24) and control group (n=12) two weeks after implantation. HIFU ablation was performed in the treatment group guided by ultrasound B. The pathological changes were observed under light microscope after hematoxylin-eosin staining and then observed under electron microscope. The activity of succinate dehydrogenase (SDH) was measured. Proliferating cell nuclear antigen (PCNA) was determined by immunohistochemistry. Results:Histopathologic observation found that the morphological features and nucleus karyotypes of tumor cells had no significant changes, but vacuole-like structure appeared in cytoplasm. Typical coagulation necrosis of tumor cells were observed under electron microscope. SDH activity detection showed that tumor cells were inactivated. The expressions of PCNA was positive in control group and negative in treatment group. Conclusion:Electron microscopy, enzyme property test, and immunohistochemistry verified that VX2 implanted breast cancer cells were dead after HIFU ablation in rabbits.

To obtain high-yield avilamycin-producing strains,low energy N~+ ion implantation technology and screening of streptomycin-re- sistant mutants are used in the study on breeding mutation.The results show that,“saddle”region,which range is from 3?10~(15) to 5?10~(15) ions/cm~2,has got better induced mutation action.It also means that the strain's resistant mutation and yield mutation closely correlate to each other,and the method of streptomycin resistant screening is feasible.We have isolated a high-yield strain SVT-45 which the productivi- ty is 195% higher than the original strain's in the rotation-flask experiments.These results showed that the ion implantation was an effective method for microbe mutagensis.

0. 05). Conclusion On the CTA exams with 64-slice spiral CT, good CTA image quality can be acquired with reduced contrast dose and saline flush, thereby we can afford reliable diagnostic information for the clinicians.

An analysis of the time pattern of outpatients visits to our hospital from 1997 to 2000 led to the discovery of the variation pattern of each outpatient departments workload. This discovery has enabled the managers of the hospital to rationally deploy the manpower and material resources of various departments, make full use of limited resources, readjust the working hours and shifts of different kinds of staff, and work out a flexible system of working hours which is more scientific and more convenient to patients. Implementation of such a system has ensured that prompt delivery of medical service is accessible to patients even at the peak of visiting hours. Hence the reduction of patients time of waiting and the provision of a scientific guarantee for establishing a quality, efficient, low consumption and fast outpatient service system, meeting the medical needs of patients to the maximum and enhancing work efficiency.

Objective To observe the changes in proliferative capability of remnant tumors implanted in rabbit breast after ablation with high intensity focused ultrasound(HIFU). Methods Rabbit implanted models were established by implanting VX_2 tumor mass into the breast of New Zealand rabbits.HIFU was applied to the tumors,and the remnant tumors were harvested by controlling temperature around probe of tumors.Fifty-six rabbits were randomly divided into control group (n=10)and HIFU group(n=40).The expression of proliferating cell nuclear antigen(PCNA)was detected by immunohistochemistry at different time points after ablation,and the survival time and organ metastasis status were observed after ablation. Results After ablation in HIFU group,the positive expression of PCNA was decreased shortly in remnant tumors,and restored to the level before ablation 21 days after treatment.The survival time in HIFU group was much longer than that in control group,and the time of lung and abdominal viscera metastasis in HIFU group appeared significantly later than that in control group(P<0.05). Conclusion Though HIFU ablation can not completely eradicate tumor after one treatment,the growth and metastasis of remnant tumors can be effectively inhibited for a short time,and HIFU ablation can prolong the survival time.

OBJECTIVETo identify triacylglycerols in coix oil.

METHODHigh performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry was used for identification. The experiment was operated under the conditions: spray voltage at 3 000 V, capillary temperature at 250 degrees C, APCI vaporizer temperature at 400 degrees C, and corona current of 4 microA. Sheath gas pressure (high purity liquid nitrogen) was 35 kPa. Mass spectra were obtained over the m/e range of 300 to 900 amu, scan duration of 1s and Q1 peak width at 0.7. The stationary phase was Zorbax Extend C18 column (4.6 mm x 250 mm, 5 microm). The mobile phase: dichloromethane-acetonitrile (35:65), flow rate: 1 mL x min(-1); column temperature: 25 degrees C.

RESULT12 triacylglycerols were identified by HPLC-MS method.

CONCLUSIONThe result can be used to identify the components in a fingerprint chromatogram of coix oil and its related injection product.

Chromatography, High Pressure Liquid ; methods ; Coix ; chemistry ; Mass Spectrometry ; methods ; Plant Oils ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control ; Seeds ; chemistry ; Triglycerides ; analysis ; chemistry ; isolation & purification

BACKGROUNDAdipocytes behave like a rich source of pro-inflammatory cytokines including monocyte chemoattractant protein-1 (MCP-1). Oxidized low-density lipoprotein (oxLDL) participates in the local chronic inflammatory response, and high-density lipoprotein could counterbalance the proinflammatory function of oxLDL, but the underlying mechanism is not completely understood. This study aimed to evaluate the effect of apolipoprotein A-I mimetic peptide L-4F on the secretion and expression of MCP-1 in fully differentiated 3T3-L1 adipocytes induced by oxLDL and to elucidate the possible mechanisms.

METHODSFully differentiated 3T3-L1 adipocytes were incubated in the medium containing various concentration of L-4F (0-50 μg/ml) with oxLDL (50 μg/ml) stimulated, with/without protein kinase A (PKA) inhibitor H-89 (10 μmol/L) preincubated. The concentrations of MCP-1 in the supernatant, the mRNA expression of MCP-1, the levels of CCAAT/enhancer binding protein α (C/EBPα), and CCAAT/enhancer binding protein β (C/EBPβ) were evaluated. The monocyte chemotaxis assay was performed by micropore filter method using a modified Boyden chamber.

RESULTSOxLDL stimulation induced a significant increase of MCP-1 expression and secretion in 3T3-L1 adipocytes, which were inhibited by L-4F preincubation in a dose-dependent manner. PKA inhibitor H-89 markedly reduced the oxLDL-induced MCP-1 expression, but no further decrease was observed when H-89 was used in combination with L-4F (50 μg/ml) (P > 0.05). OxLDL stimulation showed no significant effect on C/EBPα protein level but increased C/EBPβ protein level in a time-dependent manner. H-89 and L-4F both attenuated C/EBPβ protein level in oxLDL-induced 3T3-L1 adipocytes.

CONCLUSIONSOxLDL induces C/EBPβ protein synthesis in a time-dependent manner and enhances MCP-1 secretion and expression in 3T3-L1 adipocytes. L-4F dose-dependently counterbalances the pro-inflammatory effect of oxLDL, and cyclic AMP/PKA-C/EBPβ signaling pathway may participate in it.

3T3-L1 Cells ; Animals ; CCAAT-Enhancer-Binding Protein-beta ; analysis ; physiology ; Chemokine CCL2 ; genetics ; secretion ; Cyclic AMP ; physiology ; Cyclic AMP-Dependent Protein Kinases ; physiology ; Humans ; Lipoproteins, LDL ; antagonists & inhibitors ; pharmacology ; Mice ; Peptides ; pharmacology ; Signal Transduction ; physiology

OBJECTIVETo study the association between vitamin D receptor (VDR) gene Apa I polymorphism and vitamin D deficiency rickets in children of Shanxi Han ethnic group, and to explore the significance of individual hereditary factors in the development of rickets.

METHODSThis was a case control study. The grouping criteria were serum 25(OH)D(3) level, blood bone alkaline phosphatase and clinical symptom, respectively. The laboratory test methods were enzyme linked immunoassay and radioimmunoassay. PCR-RFLP technology was applied to examine VDR gene Apa I site polymorphism and Hardy-Weinberg hereditary balance test was used to examine the coincidence of gene distribution.

RESULTSFrequencies of AA, Aa and aa genotypes were 5.0%, 52.5% and 42.5% in the rickets group and 4.4%, 55.9% and 39.7% in the control group, respectively. Frequencies of A and a genotypes were 31.3% and 68.7% in the rickets group and 32.3% and 67.7% in the control group, respectively. There was not significant difference in the frequency distribution of VDR genotype and allelic genes between two groups (chi(2) = 0.089, P > 0.05; chi(2) = 0.028, P > 0.05). There was significant difference in the serum 25(OH)D(3) between two groups (t = -8.919, P < 0.01).

CONCLUSIONThe distribution of VDR gene Apa I polymorphism in children of Han ethnic group is balanced relatively. The Frequency of a allelic genes is 67.7% which is therefore the superior gene. VDR gene polymorphism might not be important in an individual's susceptibility to development of vitamin D deficiency.

Calcifediol ; blood ; Calcitriol ; deficiency ; Case-Control Studies ; Child, Preschool ; China ; ethnology ; Enzyme-Linked Immunosorbent Assay ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Humans ; Infant ; Male ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide ; Radioimmunoassay ; Receptors, Calcitriol ; genetics ; Rickets ; blood ; etiology ; genetics ; Vitamin D Deficiency ; complications ; genetics