1.The Mechanism of Signal Recognition and Transduction in the Establishment of Mycorrhizal Associations
Zhi-Lin YUAN ; Lian-Qing CHEN ;
Microbiology 1992;0(01):-
Multiplicity of signals and diversity of signaling pathways exist during the establishment of mycorrhizal associations together with the regulation of symbiosis-specific genes expression.This mechanism of signal recognition and transduction related with development process of the symbiont was reviewed at the molecular level.
2.Effects of the Ligustrazine Injection on Retina Injury Rat Models Induced by Retina Ischemia-reperfusion
Meilian LIAN ; Yanhui SUN ; Yuan CHEN ; Xia WANG
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(04):-
Objective To investigate the therapeutic effect of the ligustrazine injection on rats models of experimental high intraocular pressure induced by retina ischemia-reperfusion.Methods Thirty two SD rats were divided into four groups randomly:the ligustrazine group,the model group,the normal group and the vitamine E group.The ischemia-reperfusion model is established by increasing intraocular pressure to 110mmHg in rat eyes.The ligustrazine group was treated with the ligustrazine injection,the vitamine E group with gastric gavage of vitamine E,and the model and normal groups with distilled water.The period of treatment was 7 days.The histopathologic changes were observed under the optical microscope and the electron microscope,meanwhile the SOD and MDA were measured.Results After ischemia for 60 min and reperfusion for 48 h,the cells in retinal ganglion cell layer and inner nuclear layer were thinned under optical microscope,perinuclear karyopyknosis and apoptotic bodies appeared in retinal ganglion cell layer and inner nuclear layer under electron microscope,SOD activity decreased and MDA level increased.After treatment,the morphology of retinal ganglion cell layer and inner nuclear layer was improved obviously,SOD activity increased and MDA level decreased.It is suggested that ligustrazine injection exerts anti-ischemic and anti-hypoxic and cytomembrane stabilizing effects to a certain extent.Conclusion The ligustrazine injection is an effective traditional Chinese medicine in treating retinal ischemia.
3.Relationship between mathematical characteristics of cardiotocography in electronic fetal monitoring and neonatal acidemia
Lian CHEN ; Yangyu ZHAO ; Yan WANG ; Guanxiang WANG ; Yuan WEI
Chinese Journal of Perinatal Medicine 2013;16(11):656-659
Objective To explore the relationship between mathematical characteristics of cardiotocograph in electronic fetal monitoring (EFM) and umbilical blood acidosis,and help to evaluate the clinical significance of indeterminate EFM.Methods A total of 85 vaginal delivery cases (or transfer to caesarean section during labor) hospitalized in Peking University Third Hospital during February 4 to December 5,2011 was enrolled in this retrospective study.EFM monitored 0.5-1 hour before delivery was recorded and the mathematical characteristics as the baseline,deceleration area per unit time (cm2/min),deceleration duration ratio and some other variables of EFM were analyzed.All cases were divided into acidosis (n=12) and non-acidosis group (n=73) based on the umbilical blood gas results.Rank sum test or t-test were used to compare the difference on mathematical characteristics between these two groups.Results The deceleration duration ratio and deceleration area per unit time in acidosis group were 0.56±0.17 and 0.45 cm2/min (0.38-0.79 cm2/min),which were higher than those in non acidosis group [0.42±0.14 and 0.30 cm2/min (0.19-0.40 cm2/min)],the differences were statistically significant (t=2.889,Z=2.587,both P<0.05).There were no statistically significant differences in the number of mild variable deceleration and severe deceleration per unit time between acidosis group and non acidosis group [(0.44± 0.14)/min vs (0.41±0.13)/min,t=1.318,P>0.05; 0.033/min (0.016-0.062/min) vs 0.016/min (0.008-0.033/min),Z=-1.596,P>0.05].Conclusions The deceleration duration ratio and deceleration area per unit time can be used to assist the interpretation of indeterminate EFM.
4.Practice and experience of ethic review of clinical researches
Yuan CHEN ; Xueqiong ZHU ; Qingquan LIAN ; Xiaoqun ZHENG ; Jian LIN
Chinese Journal of Medical Science Research Management 2010;23(6):367-369
With the development of techniques and methods of medical researches, the ethic review of clinical research has become essential for healthy development of medical research. This article analyseds the practice of ethic reviews of research projects involving human subjects in our hospital and the difficulties in the process are discussed.
5.Ethical review of animal experiments to protect animal welfare
Yuan CHEN ; Xueqiong ZHU ; Qingquan LIAN ; Shouquan CHEN ; Xiaoqun ZHENG ; Yingying JIN
Chinese Journal of Medical Science Research Management 2012;25(2):143-144,146
Animal experiment is indispensable for biomedicine research,and contributes much to the development of biomedicine.With the development of society and advance of human civilization,the welfare of experimental animals and ethical issues of animal researches are drawing extensive attention.The current study investigated the application of experimental animals in the hospital researches,explored the relationship between animal experiment and ethics of animal welfare,and analysed the status of ethics of animal welfare.Further it discussed how to strengthen ethical review of animal experiment so as to promote the ethics management of hospital researches.
6.The changes of osteopontin expression in exprerimental murine colitis
Fengyuan CHEN ; Hongchun LIU ; Jianjun JIN ; Shiyao CHEN ; Jingjing LIAN ; Jieakesu SU ; Yuan JI
Chinese Journal of Digestion 2010;30(2):73-77
Objective To investigate the changes of osteopontin (OPN) expression in murine colitis induced by dextran sodium sulfate (DSS) and its role in inflammatory bowel disease (IBD).MethodsThirty-two male BALB/C mice were randomly assigned into 4 groups: control group (group A), DSS-induced murine colitis model group (group B), salazosulfapyridine treatment group (group C) and infliximab treatment group (group D). Plasma OPN concentration was measured by enzyme linked immunosorbent assay (ELISA). OPN mRNA level was detect by using reverse transcriptase polymerase chain reaction (RT-PCR) and protein expression in colonic tissues was analyzed by using Western blotting. The location expression of OPN in colonic tissues was determined by immunohistochemical staining. ResultsIn group A, B,C and D, the plasma concentrations of OPN were (5.26±1.93) ng/ml, (10.21±2.37) ng/ml, (4.58±1.83) ng/ml and (4.82±1.83) ng/ml,respectively: the mRNA levels of OPN in colonic tissues were (0.36±0.16), (0.71±0.17),(0.32±0.07) and (0. 34±0. 08), respectively; the protein levels of OPN in colonic tissues were (0.44±0.10), (0.85±0.04), (0.61±0.11) and (0.58±0.17), respectively. The OPN-positive cell numbers in lamina propria mononuclear were (46.6±10.9), (155.5±43.8), (73.1±6.8) and (70.6±8.3), respectively. The expression of OPN in group B was significantly higher than that in group A (P<0.05). Compared with group B, the expressions of OPN in group C and D were significantly decreased (all P valus <0. 05). No significant difference was detected between group C and D. Conclusions The study shows that the expression of OPN significantly increased in DSS-induced murine colitis and decreased after treated with drugs. OPN-mediated immune response contributes to DSS-induced murine colitis.
7.Differentiation-inducing effects of baicalin on HL-60 leukemia cells
Bu-Yuan CHEN ; Jian-Da HU ; Xin-Ji CHEN ; Lian-Huang LV ;
Chinese Journal of Pathophysiology 2000;0(08):-
AIM:To investigate the differentiation-inducing effects of baicalin on HL-60 leukemia cells.METHODS:The effect of baicalin on differential induction in AML cell line HL-60 was evaluated by cellular morphology,clone formation assay,CD11b and CD33 expression and NBT assay.RESULTS:Baicalin inhibited the proliferation of HL-60 cells.It enhanced the expression of CD11b on HL-60 cells,also increased the expression of CD33.HL-60 cells showed differentiation morphology after the drug treatment examined by Wright-Gimesa staining and NBT assay.CONCLUSION:Baicalin possessed differentiation-inducing effects on HL-60 cells.
8.Bacterial Infection in Patients with Ventilator-associated Pneumonia in ICU:Cause and Strategy
Yijuan CAI ; Zhiqun CHEN ; Jianting XU ; Guodong CHENG ; Linbao LIAN ; Bingbin YUAN ; Xiyun FAN ; Shurong LU
Chinese Journal of Nosocomiology 2006;0(05):-
OBJECTIVE To study the cause of bacterial infection of ventilator-associated pneumonia(VAP) in intensive care unit(ICU) and summarize effective methods to prevent and control the infection.METHODS Epidemiologic study on 300 patients with VAP in ICU from Dec 1,2003 to Jul 13,2006.Preventing and controlling strategy was as follows.RESULTS Pathogenic bacteria of VAP in ICU mostly were multidrug-resistant ones,of which the G-were 56.3%,G+ were 23% and fungi were 13.7%.CONCLUSIONS To control VAP in ICU proper technique and method are important.Management of hospital infection and related training of staff in ICU are the basic way.
9.Repetitive variable deceleration with a short interval in labor and neonatal acidosis
Lian CHEN ; Yan WANG ; Yangyu ZHAO ; Shufang LI ; Pengbo YUAN ; Shenglian NI ; Yan ZHAO
Chinese Journal of Perinatal Medicine 2015;18(9):656-660
Objective To discuss the relationship between repetitive variable deceleration with a short interval (RDSI) in labor and neonatal acidosis.Methods One hundred and seventy-seven electronic fetal heart monitoring (EFM) patterns within one hour preceding delivery in term singleton pregnancies were collected in Peking University Third Hospital between February 2011 to October 2013.Continued EFM were recorded before delivery.Analysis was conducted on general information of both mothers and babies,including pregnant complications,duration of labor,cord and placental factors,nature of amniotic fluid,Apgar score and neonatal cord blood gas.RDSI was defined as that over 50% intervals between two decelerations (the end of the last deceleration to the beginning of the next one) ≤ 60 s which appeared repetitevly in a period of 20 minutes.Independent sample t test,rank sum test,Chi-square test and Fisher's exact test were applied for statistics and receiver operating characteristic curve was obtained from the information of those with RDSI.Results Twenty-four of the 177 women with RDSI were assigned to the study group (24 cases,13.6%) and the rest 153 cases without RDSI to the control group.Background information of women in the two groups was comparable according to the maternal age,gestational weeks at delivery and duration of labor (all P > 0.05).The occurrence of meconium stained amniotic fluid in the study group was higher than that of the control group [16.7% (4/24) vs 5.2% (7/153),x2=5.204,P=0.045],while the pH and base excess value of the neonatal blood gas in the study group were lower [7.20 (7.13-7.28) vs 7.29 (7.25-7.33),Z=-4.490;-6.10 (-4.67 to-9.62) mmol/L vs-3.20 (-4.90 to-1.55) mmol/L,Z=-5.044;P ≤ 0.01] resulting a higher rate of neonatal acidosis [50.0%(12/24) vs 7.8% (12/153),x2=31.456,P < 0.01].No significant difference was found in the incidence of neonatal asphyxia between the two groups.The area under the curve was 0.774 (95%CI:0.579-0.969).Conclusion RDSI in labor might indicate a high risk of neonatal acidosis.
10.Intravenous leiomyomatosis of the inferior vena cava
Jian YU ; Lian YUAN ; Qingxu GUO ; Jun ZHANG ; Youdong CHEN ; Liguo YANG ; Xiaoming ZHANG
Chinese Journal of General Surgery 2008;23(4):269-271
Objective To summarize the experience on the diagnosis and therapy of intravenous leiomyomatosis(IVL)of the inferior vena cava.Methods Eight IVL patients were treated in our hospital from March 1998 to April 2007. Results The diagnosis of IVL of the inferior vena cava was established histologically by biopsy during inferior vena eavagram before operation in 4 patients.Seven patients received open surgery.Except one patient dying of massive hemorrhage during operation and one IVL recurrence during follow-up,postoperative course was uneventful and an average follow-up of 29 months found no recurrence in the other five patients. Conclusion The final diagnosis of IVL of the inferior vena cava depends on venogram and biopsy,and it is an estrogen dependent tumor originating from uterus leiomyoma.Total surgical extirpation of the tumor is the only effective treatment for IVL.