Acquired Immunodeficiency Syndrome ; Child ; Child, Preschool ; Female ; Humans ; Infant, Newborn ; Male

Morus alba L. (mulberry) is a well-known deciduous tree, belonging to the genus of Morus of Moraceae famlily. Its leaves, twigs, roots (bark) and fruits are widely used in the traditional Chinese medicine. The active constituents of mulberry contained flavonoids, alkaloids, steroids, coumarins, with the significant hypoglycemic, hypolipidemic, antihypertension, anti-oxidation, anti-inflammatory, anti-bacterial, anti-tumor and immunomodulatory activities. This review summarized the research progress of the major pharmacological activity, pharmacokinetics and drug-drug interaction based on CYPs and transporters of mulberry and its active constituents.
Alkaloids ; pharmacology ; Coumarins ; pharmacology ; Flavonoids ; pharmacology ; Fruit ; chemistry ; Herb-Drug Interactions ; Humans ; Medicine, Chinese Traditional ; Morus ; chemistry ; Plant Extracts ; pharmacology ; Plant Leaves ; chemistry ; Plant Roots ; chemistry ; Steroids ; pharmacology

[Objective] To establish a method to seperate rat mesenchymal stem cells(rMSCs) from rat marrow, culture rMSCs in vitro, and study its biological characteristics. [Methods] rMSCs were seperated from rat marrow with wall-sticking method and expanding cultured in vitro. To draw the growth curves of cells at 1th, 3th, 5th passage, detect the surface antigens with flow cytometry. To evaluate the effect of fetal calf serum with different concentrations to rMSCs. [Results] There were approximately (5～6)?105 cells after primary culture and over (2~3)?108 rMSCs at 5th passage. The appearance of rMSCs is like shape of spear and its growth curves are like shape of S. The rMSCs were positive for CD90 and negative for CD45. [Conclusions] Cells seperated and cultured by the method established in this experiment were rMSCs. They have the biological characteristics of rMSCs, and are good resources for study in tissue engineering.

Medicine education must pay attention to humanity education,which is determined by the nature of medicine.The present situation and problems of the medical humanity education in china were reviewed and new approaches to improve long-term mechanism of the medical humanity education in china were enhanced from the following four apects:constructing teaching staff,setting up curriculum,reforming teaching methods and building campus-culture.

Drug combination is extensive in the clinical real world,which is an important part and the inherent requirements of the post-marketing evaluation of traditional Chinese medicine (TCM). The key issues and technology include multi-domain and multi-disciplinary such as the rationality, efficacy and safety evaluation of combination drug starting from clinical real world, study on component in vivo and mechanism of combination drug, the risk/benefit assessment and cost-benefit evaluation of combination drug and so on. The topic has been studied as clinical demonstration on combination therapy of variety of diseases such as coronary heart disease, stroke, insomnia, depression, hepatitis, herpes zoster, psoriasis and ectopic pregnancy. Meanwhile, multi-disciplinary dynamic innovation alliance of clinical drug combination has been presented, which can promote the academic development and improving service ability and level of TCM.
Drug Combinations ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; methods

Aim To study the effect of emodin on IL-8 secretion and NF-?B activation of HT-29 cells,and explore the molecular mechanism of emodin.Methods The cytotoxicity of emodin was assessed by WST;NF-?B activation was detected with co-focal microscopy by immunofluorescence;the production of IL-8 was investigated by ELISA.Results Emodin with the concentration of 10~80 ?mol?L-1 could decrease the mass production of IL-8 Secretion of HT-29 cells stimulated by IFN-?+LPS in a dose-dependent manner.Emodin with various concentrations could inhibit NF-?B activation dose-dependently.Conclusions Emodin inhibited IL-8 secretion and NF-?B activation of HT-29 cells stimulated by IFN-?+LPS.

Objective To explore the relationship between mathematical characteristics of cardiotocograph in electronic fetal monitoring (EFM) and umbilical blood acidosis,and help to evaluate the clinical significance of indeterminate EFM.Methods A total of 85 vaginal delivery cases (or transfer to caesarean section during labor) hospitalized in Peking University Third Hospital during February 4 to December 5,2011 was enrolled in this retrospective study.EFM monitored 0.5-1 hour before delivery was recorded and the mathematical characteristics as the baseline,deceleration area per unit time (cm2/min),deceleration duration ratio and some other variables of EFM were analyzed.All cases were divided into acidosis (n=12) and non-acidosis group (n=73) based on the umbilical blood gas results.Rank sum test or t-test were used to compare the difference on mathematical characteristics between these two groups.Results The deceleration duration ratio and deceleration area per unit time in acidosis group were 0.56±0.17 and 0.45 cm2/min (0.38-0.79 cm2/min),which were higher than those in non acidosis group [0.42±0.14 and 0.30 cm2/min (0.19-0.40 cm2/min)],the differences were statistically significant (t=2.889,Z=2.587,both P＜0.05).There were no statistically significant differences in the number of mild variable deceleration and severe deceleration per unit time between acidosis group and non acidosis group [(0.44± 0.14)/min vs (0.41±0.13)/min,t=1.318,P＞0.05; 0.033/min (0.016-0.062/min) vs 0.016/min (0.008-0.033/min),Z=-1.596,P＞0.05].Conclusions The deceleration duration ratio and deceleration area per unit time can be used to assist the interpretation of indeterminate EFM.

Adolescent ; Calculi ; Humans ; Male ; Nose Diseases ; Turbinates

Objective To study the effects of Bis(maltolato) oxovanadium(BMOV) on renal structure of spontaneously hypertensive rats(SHRs) and its mechanism.Methods Thirty-six SHRs were divided into three groups at random: blank group,Nifedipine group and BMOV group.The light microscope and electron microscope were used to examine the pathologic changes of the kidney in rats.Results The rat glomerular mesangial expansion was found and the lumen of glomeruler capillary nearly closed in bland group,while in BMOV group the pathological changes mentioned above were unobvious.Conclusion BMOV may have protective effects on kidney in SHRs.