1.Nosocomial Infection Control in Whole Process of Blood Collecting on a Blood Collecting Vehicle
Chuanqiao LIU ; Lian XUE ; Zhian ZHANG
Chinese Journal of Nosocomiology 2006;0(07):-
OBJECTIVE To control the nosocomial infection in the whole process of blood collecting on a blood collecting vehicle.METHODS To supervise the technological sanitation of blood collecting environment,increase primary assay of ALT,using laser beam apparatus instead of needle puncture,select the dynamic disinfection cleaning machine instead of classical tri-oxygen or ultraviolet lamp disinfection,and standardize blood collecting procedure and environmental disinfection.RESULTS After 110 thousand times a year of blood collecting on the blood collecting vehicle in Wuhan there were no one nosocomial infection happened.CONCLUSIONS The nosocomial infection in the whole process of blood collecting on a blood collecting vehicle can be prevented by the quality control of environmental condition and improvement of assay installation.
2.Culture of cryopreserved mouse spermatogonial stem cells.
Lian-jun LI ; De-xue LI ; Xue-ming ZHANG
Chinese Journal of Applied Physiology 2004;20(2):145-208
Animals
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Cell Culture Techniques
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methods
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Cells, Cultured
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Cryopreservation
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Male
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Mice
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Spermatogonia
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cytology
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Stem Cells
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cytology
3.Expression of PKCθ in peripheral blood mononuclear cells of aplastic anemia patients and its effects on Th1 and Tc1 cells.
Xue-jing YANG ; Wei-hua ZHANG ; Xiu-lian ZHANG
Chinese Journal of Hematology 2012;33(11):951-953
Adolescent
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Adult
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Anemia, Aplastic
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blood
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Case-Control Studies
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Female
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Humans
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Isoenzymes
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metabolism
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Leukocytes, Mononuclear
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metabolism
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Male
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Middle Aged
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Protein Kinase C
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metabolism
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Protein Kinase C-theta
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T-Lymphocytes, Cytotoxic
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cytology
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Th1 Cells
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cytology
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Young Adult
4.Effect of iohexol on the renal function in children undergoing cardiac angiography.
Xin-Ying ZHANG ; Yu-Lin WANG ; Xue-Lian ZHU
Chinese Journal of Contemporary Pediatrics 2009;11(11):935-936
Child
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Child, Preschool
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Contrast Media
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adverse effects
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Coronary Angiography
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Female
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Humans
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Infant
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Iohexol
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adverse effects
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Kidney
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drug effects
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physiology
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Male
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beta 2-Microglobulin
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urine
5.Therapeutic efficacy of moxibustion at different distances on type Ⅱ collagen-induced arthritis
Yang WANG ; Xinyu WU ; Zhiling SUN ; Yongyi ZHANG ; Lian XUE ; Xue QIN
Chinese Journal of Tissue Engineering Research 2017;21(8):1241-1245
BACKGROUND: Moxibustion can improve the symptoms of rheumatoid arthritis and reduce inflammation, but there are no uniform operation standards. The moxibustiondistance becomes one of factor influencing the therapeutic efficacy.OBJECTIVE: To compare the effects of different moxibustion distances on rheumatoid arthritis, and to explore the optimal distance.METHODS: The 8 of 40 male Sprague-Dawley rats were randomlyselected as controls. The other 32 were used to make animal modes of collagen type Ⅱ-induced ankle arthritis, and then given moxibustion at Shenshu (BL23) and Zusanli (ST36) at an interval of 1, 2 and 3 cm, respectively, once daily, 10 minutes at each point, 6 days per course for three courses, with a course interval of 1 day. Model rats with no treatment acted as model group.RESULTS AND CONCLUSION: Compared with the control group, the toe volume, arthritis index and serum levels ofinterleukin-1β and tumor necrosis factor-α except the body mass were significantly increased in the model group (P < 0.01).After moxibustion treatment, these indexes were significantly decreased in the three treatment groups (P < 0.01), especially in the 1 and 2 cm groups (P < 0.01). Narrowed articular cavity, intra-articular inflammation and pannus formation were observed in the model group, while only moderate inflammatorycell infiltration and few pannus formation were found in the 1,2, 3 cm groups. These results indicate that moxibustion improvesjoint functions and regulates immune reaction by downregulating the levels of interleukin-1β and tumor necrosisfactor-α, as well as reducing synovial hyperplasia. In addition,the optimal distance for moxibustion is 1 or 2 cm, which is amomentous proposition to improve therapeutic efficacy.
6.Experimental study on the effect of different moxibustion durations on rats with rheumatoid arthritis
Xinyu WU ; Yang WANG ; Zhiling SUN ; Xue QIN ; Jiang ZHAO ; Xiao XU ; Yongyi ZHANG ; Lian XUE
Journal of Acupuncture and Tuina Science 2017;15(3):177-183
Objective: To observe the effect of different moxibustion durations on rats with rheumatoid arthritis (RA) and to evaluate the relationship between moxibustion amount and moxibustion efficacy.Methods: Eight rats were randomly selected as a normal group from the 40 male Sprague-Dawley (SD) rats, and the other 32 rats were used to establish typeⅡ collagen-induced RA models. After successful modeling, the 32 rats were randomly divided into a model group, a moxibustion for 20 min group, a moxibustion for 40 min group and a moxibustion for 60 min group, with 8 rats in each group. Rats in the normal group did not receive modeling and moxibustion intervention; rats in the model group did not receive moxibustion after modeling; rats in the moxibustion for 20 min group, the moxibustion for 40 min group and the moxibustion for 60 min group were treated with moxibustion at Shenshu (BL 23) and Zusanli (ST 36) for 20 min, 40 min and 60 min, respectively. Six days were a course of treatment, with a total of 3-course treatments and a 1-day rest between the courses of treatment. After treatment, the serum levels of interleukin (IL)-1β and tumor necrosis factor (TNF)-α, arthritis index (AI) scores, toe volumes and pathological score of synovitis were evaluated in the rats.Results: Compared with the normal group, the serum IL-1β and TNF-α levels, and the toe volumes in the model group were increased, and the differences were statistically significant (P<0.01), before the treatment. Compared with the model group, the serum IL-1β and TNF-α levels, toe volumes and arthritis index (AI) scores were significantly decreased in the moxibustion for 20 min group, the moxibustion for 40 min group and the moxibustion for 60 min group (P<0.05 orP<0.01 ). Compared with the moxibustion for 20 min group and the moxibustion for 60 min group, serum IL-1β and TNF-α levels, toe volumes and AI scores were decreased more significantly in moxibustion for 40 min group, and the differences were statistically significant (P<0.05 orP<0.01). There were no significant differences in serum IL-1β and TNF-α levels, AI scores and toe volumes between the moxibustion for 20 min group and the moxibustion for 60 min group (allP>0.05). The synovial histopathological improvement was the most obvious in the moxibustion for 40 min group, when the synovial histopathological changes were compared among the moxibustion for 20 min group, moxibustion for 40 min group and moxibustion for 60 min group.Conclusion: The therapeutic efficacy of moxibustion for 40 min in RA rats was more significant than that of moxibustion for 20 min and moxibustion for 60 min, indicating that the duration of moxibustion is the main factor affecting its therapeutic efficacy.
7.Clinical observation on effect of combination of zhuanyindan and hormone in treating male infertility with positive antisperm antibody.
Ai-lian YU ; Feng-zhen ZHANG ; Feng-xue ZHANG
Chinese Journal of Integrated Traditional and Western Medicine 2004;24(3):223-226
OBJECTIVETo explore the therapeutic effect of combination of Zhuanyindan (ZYD, a self-made Chinese herbal preparation) and hormone in treating male infertility with positive antisperm antibody and its influence on nitric oxide (NO) level.
METHODSEighty-two patients were randomly divided (according to the digital list) into the WM group (n = 20, treated with prednisone), the TCM group (n = 28, treated with ZYD) and the ICWM group (n = 34, treated with prednisone plus ZYD). The clinical effect, negative converting rate of antisperm antibody, changes of NO level in semen and various parameters of sperm motion before and after treatment were observed.
RESULTSThe total effective rate in the ICWM group was 88.2%, that in the TCM group 75.0% and in the WM group 65.0%. Significant difference was seen in the ICWM and TCM group before and after treatment in NO level, sperm motion parameters, including linear motion speed, linearity, propulsion, whip frequency, sperm vitality and mean moving angle, and quality of semen (P < 0.05 or P < 0.01). In the WM group, significant difference in comparison before and after treatment was seen in NO level, propulsion, whip frequency, mean moving angle and quality of semen, including vitality and survival rate (P < 0.01).
CONCLUSIONCombination of Chinese herbs and hormone could lower the NO level in semen and improve the quality of sperm.
Adult ; Autoantibodies ; metabolism ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Infertility, Male ; drug therapy ; etiology ; immunology ; Male ; Nitric Oxide ; metabolism ; Phytotherapy ; Prednisone ; therapeutic use ; Semen ; metabolism ; Sperm Motility ; drug effects ; Spermatozoa ; immunology
8.Effect of total ginsenosides on protective enzymes of Mythimna separata larvae.
Shi-qiang TAN ; Lian-xue ZHANG ; Lin MA ; Ai-hua ZHANG
China Journal of Chinese Materia Medica 2014;39(22):4283-4287
Under indoors simulating natural growing condition, the 4th-instar Mythimna separata larvae were fed by using poi- son leaf disk method. The effect of total ginsenosides on the protective enzymes (PPO, T-SOD, CAT and POD) of M. separata larvae was studied. The total ginsenosides could influence the protective enzymes of 4th-instar M. separata larvae significantly. After treated by total ginsenosides, the PPO activities increased firstly then decreased, and tended to equilibrium, and reached the maximum after 48 h. Furthermore, the total ginsenosides disturbed the dynamic balance of SOD, CAT and POD of M. separata larvae, and the yield of O2-* speeded. The results suggest that the total ginsenosides influence the protective enzymes of 4th-instar M. separata larvae, and disturb the original dynamic balance of protective enzymes. Consequently the insect suffers from the harm of O2-*.
Animals
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Enzymes
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metabolism
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Ginsenosides
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metabolism
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Larva
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metabolism
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Lepidoptera
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metabolism
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Oxygen
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metabolism
9.The effect of implantation approach on bone marrow mesenchymal stem cells implanted into the infarct myocardium
Feng LIAN ; Song XUE ; Xuejun WU ; Ruolan ZHANG ; Xiaomei WANG ; Hongsheng ZHU
Clinical Medicine of China 2009;25(9):897-899
Objective To study the effect of different approach on myocardial contractile force, angiogenesis and collagen renovation in acute myocardial infarct site by implantation of bone marrow mesenchymal stem cells (MSCs). Methods 32 Gui-zbou Xiang porcine were randomly divided into control group (group C), intra-coronary arterial injection group (group A) ,topical injection group (group T) and intra-coronary arterial + topical injection group( group A + T). 3 ml bone marrow was extracted. MSCs were cultured according to the methods of Wakitani's. After being co-cultured with 5-azacytidine for 24 hours, these cells were labelled with bromodeoxyuridine ( BrdU ). Autologus MSCs were implanted into the acute myocardial infarct site via the distal segment of the ligated left anterior descending artery (LAD) ,topical injection and both. Specimens and muscle strip were harvested in 3 and 6 weeks after MSCs implantation for microvessel count, P Ⅲ NP activity and contractile force measurement. Results The contractile force(48.6±5.9) %, (42.1±6.2 ) %, ( 56.9±5.1 ) %, microvessel count ( 19.6±4.3,17.1±4.0, 23.2±5.5 ) and collagen renovation (4.6±0.5 ) μg/L, ( 5.9±0.7 ) μg/L, ( 3.9±0.3 ) μg/L in the cell implant groups was better than that in the control group(37.9±5.4)% ,13.2±3.8,(8.7±0.8) μg/L,(P <0.01) ,espe-cially in group A + T. Conclusions Implantation of MSCs through intra-coronary arterial plus topical injection of MSCs maybe an ideal approach for infracted myocardium.
10.Effects of N-acetylcysteine on Alcoholism in Mice
Yongping HUANG ; Shiwen ZHOU ; Ying XU ; Jianlin TANG ; Lian WANG ; Jinyuan ZHANG ; Xue YANG
China Pharmacy 2001;0(07):-
OBJECTIVE:To investigate the effects of N-acetylcysteine(NAC)on the concentration of ethanol in serum and the activities of alcohol dehydrogenase in liver and stomach tissue in alcoholic mice.METHODS:All mice were given p.o white spirit30min after p.o saline or5%NAC solution,and the durations from the disappear of righting reflex to recovery in mice were recorded and the number of dead mice in24hours were countered;With the same procedure for six days,the mice were killed by withdrawaling blood from orbit,and taken out the liver and stomach immediately,the concentrations of ethanol in serum and the activities of alcohol dehydrogenase in liver and stomach were measured by chromatometry and colorimetric method respectively.RESULTS:In drunk test,5%NAC solution significantly postponed the time from p.o white spirit to the disappear of righting reflex(P