Aged ; Female ; Humans ; Male ; Middle Aged ; Silicosis

The MPN method was used to enumerate ammonia-oxidizing bacteria (AOB) in water and sediments of several shallow lakes. The suitable incubation time, medium types and substrate (ammonium sulphate) concentrations were studied. The results showed that, MPN values increased with the incubation time, reaching a stable maximum at some time stages, which was 40 days in all the samples for MSF medium. Among the three media used (XZ-AOB、MSF、SW), MSF give the highest MPN value. In addition, am- monium sulphate concentration in medium was an important factor affecting MPN estimation of AOB. Compared to AOB in lake sediments, AOB in lake water was more sensitive to ammonium sulphate concentration.

Objective Micronucleus test(MNT) was used to evaluate the single and combined genotoxicity of dichlorvos(DDVP),dimethoate(DM) and malathion(Mal) in HepG2 cell line,and 2?2 factorial design was adopted to elucidate the combined genotoxic effects.Methods In cytotoxicity test,HepG2 cells were exposed to the three OPPs(DDCP,DM,Mal) for 4 h respectively,and the doses at which cell viability above 80% were selected for the MNT,DDVP:3.125-25 ?g/ml,DM:25-200 ?g/ml,Mal:50400 ?g/ml,the micronucleated cell(MNC) rates and the replicative index(RI) were calculated.The combined genotoxicity of them was investigated with their doses as follows:low dose(DDVP:3.125 ?g/ml,DM:25 ?g/ml,Mal:50 ?g/ml);high dose(DDVP:12.5 ?g/ml,DM:100 ?g/ml,Mal:200 ?g/ml).Results In MNT,after treatment of HepG2 cells with DDVP,DM or Mal alone for 4 h,the MNC rates were increased in a dose-response manner(DDVP:r=0.955,P

Objectives To understand the susceptible factors for tobacco use among doctors in Henan province.Methods A random multilevel cluster sampling method was conducted.2 990 subjects were studied with an inquiry and self-reported questionnaire.Results The total smoking rate was 27.5%.The current smoking rate was 22.3%.Daily smoking rate was 13.3%.The main reasons for smoking were social needs,pressure relief,getting refreshing.About 21.4% subjects smoked in front of patients.Majority doctors understood the relationship between smoking and some diseases.The multivariate analysis showed that the doctors'smoking behavior was related with his gender,age,education background,monthly revenue,working section,hospital administration level,knowledge scale of tobacco being harmful on health,knowledge scale on the relationship between smoking and illness,doctors'social norm attitude for smoking.Conclusions The smoking behavior of doctors was complex,which was related to personal,social environment factors.Only comprehensive smoking prevention interventions could be stressed to produce lasting behavior change.

Objective To study the effect of climate change on physiological indexes of elderly people.Methods From January,2006 to January,2007,30 elderly couples were selected and blood pressure,pulse,body weight were investigated.The weather data was collected.Results The lowest value of body weight appeared in February in normotensive subjects,in September in hypertension subjects.The lowest value of body temperature in two groups appeared in February and March.The lowest value of pulse appeared in March and April in normotensive subjects,in September in hypertension subjects.The curve of blood pressure change(SBP and DBP) presented"V"in two groups.The lowest value appeared in summer(July-September).The highest value appeared in winter(December-February).The blood pressure in winter was higher than that in summer(P

Objective To detect the expression of RXR? gene protein in gastric cancer, superficial gastritis, normal gastric mucosa, in order to explore the relationship between the development of gastric cancer, combined with clinical and pathological analysis of its clinical significance. Methods Using immunohistochemical detection RXR? protein in gastric cancer, superficial gastritis, gastric mucosa in the normal expression. Results RXR? protein mainly located in the nucleus of cells, there are a small number of cytoplasmic staining, a brown or yellow granular, in gastric cancer, superficial gastritis, normal gastric mucosa, RXR? protein expression rates were 25.0%, 81.1%, 96.1%; group and the normal mucosa and superficial gastritis, gastric cancer group RXR? expression rate has dropped, there were significant differences (P0.05). The lymph node metastasis and tumor pathology and clinical phases closely related (P

Objective To study the inhibition of HCV IRES mediated HCV core protein expression in cells by inhibitor RNA. Methods Plasmid pcRz-IRNA, a eukaryotic expression vector with IRNA and two self cleavage ribozyme overhang at both sides respectively, was constructed and co-transfected with pcHCVcluc (containing HCV NCR, core and Luc genome) into the HHCC cell line (Human Hepatocellular Carcinoma cell line). Immunoflurescence tests were applied to detect the co-transfected cells, which were thereafter analysed with confocal microscope quantitatively. Luciferase activity was valued using Luc Assay System (Promega). Results The cotransfected cells expressed HCV core protein, and the fluorecein in which was reduced significantly in comparison with control. Conclusions IRNA can inhibit the expression of HCV IRES mediated core protein in the cotransfected cells.

Objective To investigate the effects of constant magnetic field on secretion and expression of vascular cell adhesion molecule-1 (VCAM-1) in human vascular smooth muscle cells (VSMCs) induced by angiotensin Ⅱ (AngⅡ). Methods The fourth to sixth passages of in vitro cultured VSMCs from human umbilical artery were used. The cells were divided into six groups, i.e. control group, AngⅡ (1?10 -6mol/L) group, Ang Ⅱ with exposure to 1, 5, 10 or 50 Gs of constant magnetic field groups. Samples were collected 12 hours after intervention. The secretion of VCAM-1 was assayed by enzyme linked immunosorbent assay (ELISA) and the expression of VCAM-1 was assessed by immunocytochemistry. Results The secretion of VCAM-1 was increased significantly 12 hours after intervention with AngⅡ of 10 -6mol/L (P

Objective To study the curative effect of endoscopic dilatation in children with benign esophageal stricture. Methods 98 patients with benign esophageal stenosis from June 2013 to June 2016 were selected as the subjects of group A, group B and group C, 11 patients in group A were reflux esophageal stenosis, 43 children in group B were congenital esophageal atresia and in group C were 44 patients with chemical burn esophageal stricture. Then compare the three groups of children before the expansion of stenosis before the situation, the number of expansion and complications occurred. Results There was no significant difference in the average diameter of stenosis among the three groups (P > 0.05). The average length of the children in group C was higher than that in group A and group B, and group B was higher than group A, the difference was statistically significant (P > 0.05). The average number of expansion in group C was higher than that in group A and group B, and group B was higher than that in group A and group B higher than group A, the difference was statistically significant (P < 0.05). The incidence of complications in group C was higher than that in group A and B. The difference was statistically significant (P < 0.05). There was no significant difference in the incidence of complications between group A and group B (P > 0.05). Conclusion Endoscopic dilatation has a good clinical effect on children with benign esophageal stricture, but children with chemical burn esophageal stricture need to be expanded more often, while the complications are higher and the treatment is more difficult.

Aim To investigate the Clotrimazoles (CLT) inhibitive effect on the secretion of ?-defensins of the Paneth cells.Methods The ?-defensin mRNA expression is determined by semi-quantitative RT-PCR. The secretion of defensins is determined by dot-blot and ELISA. Results CLT has no influence on the defensin mRNA expression. All of 125 nmol?L -1, 250 nmol?L -1 and 500 nmol?L -1 CLT can inhibit the secretion of ?-defensins. Conclusion CLT could inhibit the secretion of defensins, which suggests that CLT maybe influence the function of small intestine