Adolescent ; Aspirin ; therapeutic use ; Child, Preschool ; Diospyros ; adverse effects ; Female ; Fruit ; adverse effects ; Gastrointestinal Diseases ; drug therapy ; Humans ; Lithiasis ; drug therapy ; Male ; Treatment Outcome

BACKGROUND: The stress can induce sympathic nervous-adrenalmedulla system to release a lot of noradrenalin(NE) and adrenalin(E),pro mote bone marrow hemopoietic stem cells to differentiate towards lympho cyte and plasmocyte,decrease the number of granulocyte,thus organism canadapt to surrounding stress,so as to protect critical organs from damage. OBJECTIVE: To observe effect of confinement and vibration stress on in dexes of hematology and myelogram in Chinese tree shrews. DESIGN: Sex-marched and controlled experiment. MATERIALS: Thirty Chinese tree shrews (15 females, 15 males) weigh ing 120 g to 140 g were selected. Self-made experimental cylinders withmany vent-holes, 16 cm length and 6cm inside diameter,were used, one sideof which was sealed with plexglass,and the other side was blocked by mov able plexiglass for animals in and out.The air bath and homothermal vibra tor(Type SHZ-82,made in Changzhou,China). METHODS: The experiment was completed at the Experimental Center ofPublic Security, Gansu Political Science and Law Institute from March toOctober in 2003. ①Thirty Chinese tree shrews were randomly divided intofive groups(n=6):The animals in the control group(C group) were fed nor mally; The experimental groups consisted of reserpine + vibration and vi bration groups. The animals in the reserpine + vibration (R+V)group wereadministered reserpine (0.05 mg/kg, im.) and vibrated at 140 beats/min; the vibration groups (V1,V2,V3) were vibrated at 70, 140, 210 beats/min, respectively. ② The animals in experimental groups were individually keptin the experimental tubes for 5 minutes, then put the tubes into the air bath and homothermal vibrator for 2 minutes. ③ The bleeding time, bloodsedimentation, clotting time, and indexes of hemogram were determinedwith the methods of Duke, Westergren, glass capillary, and full-automaticbiochemical instruments (type 721, Japan), respectively. ④ Dunnett ANO- VA was used to compare the differences between groups . MAIN OUTCOME MEASURES: Comparison of indexes of hematologyand myelogram in Chinese tree shrews betweem the four groups.RESULTS: Thirty Chinese tree shrews were involved in the final analysis.① Indexes of hematology: compared with those of C group, the bleeding time of the V2 group significantly prolonged by 104.8% (P＜0.01 ),but the clotting time and blood sedimentation decreased by 37.3% and 7.5% (P＜0.01 ), respectively. Also, the counts of red blood cells, reticulocyte, blood platelet and white blood cells,as well as content of hemoglobin increased by i7.5% ,50.9%, 11.8%, 10.3% and 13.7% ,respectively.There was no significant difference between R+V group and C group(P ＞0.05),and also in various vibration groups. ②Indexes of myelogram:compared with those of C group,counts of granulocyte were decreased by 31.5%(P＜0.01),counts of red cells,lymphocyte,mononuelear,plasmocyte,megakaryocyte,mitotic cells increased by 25.7% ,77.6%,213.3% ,65.8% ,100%,161.3%,respectively (P＜0.01); those in R+V group were similar to C group (P＞0.05); There were no significant differences among various vibration groups(P＞0.05).CONCLUSION: Confinement and vibration stress can increase significantly the blood cells and reduce counts of granulocyte in bone marrow,and the above changes can be interfered by reserpine.

Acid a-naphthyl acetate esterase (ANAE), a cytoplasmic marker was used to identify the T lymphocytes and their subpopulations in peripheral blood of mice experimemally infected with T. spiralis. The results showed that the absolute number of lymphocytes and T lymphocytes increased in parallel on d, after infection, reachiug the peak on d14, and remaining above normal level on d60. The spotted granular ANAE positive cells (Helper T cells, Th) were decreasing and the scattered granular ANAE positive cells (suppressor T cells, Ts) were increasing, leading to a lowering of the Th/Ts ratio which was most marked on d14 and returned to normal on d60. The depres-sion of host immune function during T. spiralis infection might be related to the lowering of Th/Ts ratio.

Aim To observe the cytotoxic and proliferous effects of Nitraria tangutorum flavone(NTF)on human A-704 strain cells.Methods In vitro cultured human A-704 strain cells,the cytotoxic and proliferous effects of NTF on human A-704 strain cells were determined with methods of trypan-blue and clone formation,and the microelement in cell was detected with X-electron microscope scanning.Results ① The trypan-blue detection showed that the living cells in the NTF group(0.1,0.5 and 5 mg?L-1)and the 5-FU group were decreased by 33.8%~57.3%,35.8%~80.1%,60.8%~99% and 40.3%~59.6% at 6~18 hour after treatment,which significantly differed from those of the control group(P

Objective To investigate the effect and mechanism of probiotic products on alpha-naphthylisothiocyanate(ANIT) induced intrahepatic cholestasis of rats.Methods Seventy-two SD rats were randomly divided into 3 groups: control group(n=8),intoxication group(n=32) and intervention group(n=32).After 3 days adapted feeding,all rats received a single intragastric administration of ANIT(200 mg/kg) to induce acute intrahepatic cholestasis.Probiotics[4.2?108/(kg?d)] was given to intervention group 2 days before ANIT was administrated.After ANIT was given,biliary flow,alanine aminotransferase transaminase(ALT),total bilirubin(TB) and gastrointestinal hormone were recorded in every 48 h till 192 h.SPSS 12.0 software was used to analyze the data.Results 1.Forty-eight hours after ANIT was gavaged,the biliary flow of intoxication and intervention groups were lowered while ALT and TB were increased.But the above observation items of intoxication group were more significantly altered than those of intervention group.Then,they all recovered gradually.Those of intervention group returned to nearly normal levels 144 h after ANIT was gavaged while those of the intoxication group recover at 192 h.2.After ANIT was gavaged,MTL of intoxication and intervention groups was lowered while vasoactive intestinal peptide(VIP) increased.But the above items of intoxication group were more significantly altered than those of intervention group.Then,they all recovered gradually and the intervention group was much more early to returned to normal levels than the intoxication group at 192 h.Conclusions Probiotics can promote the bowel movement through increasing the secretion of gastrointestinal hormone,then relieve the cholestasis.

Aim To investigate the Clotrimazoles (CLT) inhibitive effect on the secretion of ?-defensins of the Paneth cells.Methods The ?-defensin mRNA expression is determined by semi-quantitative RT-PCR. The secretion of defensins is determined by dot-blot and ELISA. Results CLT has no influence on the defensin mRNA expression. All of 125 nmol?L -1, 250 nmol?L -1 and 500 nmol?L -1 CLT can inhibit the secretion of ?-defensins. Conclusion CLT could inhibit the secretion of defensins, which suggests that CLT maybe influence the function of small intestine

Embolism, Paradoxical ; complications ; Female ; Humans ; Middle Aged ; Myocardial Infarction ; etiology

Accidents, Occupational ; statistics & numerical data ; Humans ; Poisoning ; epidemiology

[Objective] To establish a method to seperate rat mesenchymal stem cells(rMSCs) from rat marrow, culture rMSCs in vitro, and study its biological characteristics. [Methods] rMSCs were seperated from rat marrow with wall-sticking method and expanding cultured in vitro. To draw the growth curves of cells at 1th, 3th, 5th passage, detect the surface antigens with flow cytometry. To evaluate the effect of fetal calf serum with different concentrations to rMSCs. [Results] There were approximately (5～6)?105 cells after primary culture and over (2~3)?108 rMSCs at 5th passage. The appearance of rMSCs is like shape of spear and its growth curves are like shape of S. The rMSCs were positive for CD90 and negative for CD45. [Conclusions] Cells seperated and cultured by the method established in this experiment were rMSCs. They have the biological characteristics of rMSCs, and are good resources for study in tissue engineering.

AIM To study the inhibitory effect and mechanism of n itrary flavone in Hep and U 14 tumor. METHODS The Hep or U 14 tumor cells were injected in the armpit subcutaneous layer of right foreleg of BALB/c strain mice. After 24 h,nitrary flavone at a does of 100 mg?kg -1 ?d -1 body weight or combine nitrary flavone with 5-Fu(100 mg?kg -1 ?d -1 body weight)was given (ip) and inhibitory rate of nitrary flavone for transplanted tumor and the immune function of mice were determined. RESULTS Administration of nitrary flavone to mice for 10 consecutive days exhibited significantly the inhibitory rate for Hep and U 14 by 34 11%,32 14% and 50 73%,47 22%,respectively, as well as the weight of immune organs of mice, formation value of serolysin and phagociytic index were significantly enhanced(P