OBJECTIVETo observe the effect of Kuntai Capsule (KC) on the number of retrieved oocytes, the quality of high-quality oocytes and embryos in in vitro fertilization of poor ovarian response (POR) patients.

METHODSTotally 70 POR patients preparing for in vitro fertilization-embryo transfer (IVF-ET) were randomly assigned to the observation group and the control group, 35 cases in each group. KC was administered to patients in the observation group in the preparation cycle (i.e., three menstrual cycles before IVF-ET) and during the superovulation process. Those in the control group took placebo during this period. Before and after medication the improvement of Shen yin deficiency syndrome (SYDS) was observed in the two groups. The basal follicle-stimulating hormone (bFSH), luteinizing hormone (LH), estradiol (E2), anti-Mullerian hormone (AMH), the ratio of FSH to LH, and antral follicle count (AFC) were observed. Besides, the E2 level of a single ovum on the day of HCG injection, the number of retrieved oocytes, the high-quality oocyte rate, and the high-quality embryos were observed.

RESULTSCompared with the control group, the SYDS, decreased bFSH and LH levels, increased ACF numbers, the E2 level of a single ovum on the day of HCG injection, the number of retrieved oocytes, high-quality oocytes, and high-quality embryos were superior in the observation group (P < 0.05). There was no statistical difference in the decreased FSH/LH level (P > 0.05). E2 and AMH increased after medication of KC in the observation group, while they decreased after administration of placebos in the control group. There was statistical difference in the post-pre treatment difference of E2 and AMH between the two groups (P < 0.05).

CONCLUSIONKC could increase the number of retrieved oocytes, and elevate the quality of occytes and embryos in the IVF-ET.

Adult ; Drugs, Chinese Herbal ; pharmacology ; Embryo Transfer ; Female ; Fertilization in Vitro ; Humans ; Oocyte Retrieval ; Oocytes ; drug effects ; Pregnancy

Embryonic stem cells(ESCs),a kind of totipotent cells,can differentiate into cell type of the 3 germ layers.Adult stem cells(ASCs) are pluripotent stem cells,which can self-renew and transdifferentiate into a completely different tissue derivative cells.Thus ASCs can transdifferentiate into germ cells in a certain condition.Great progress has made for the study on human ESCs.However,use of ESCs faces the immunological rejection,oncogenicity and ethical controversies problems,and these limit their uses for treatment of human diseases.On contrast,ASCs can advoid these problems,and they may have a better perspective.Germ cells as carriers of genetic materials,are worth of studying.If male germ cells can be derived from ASCs,this will open the possibilities for the use of these cells for male infertillty in clinical application.In this review,we will introduce the progress on derivation of male germ cells from ESCs and ASCs.

Diabetic Neuropathies ; blood ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Nitric Oxide ; blood ; Phytotherapy

Objective To compare the efficacies of exposure to cigarette smoke alone and in combination with intra-tracheal injection of lipopolysaccharide in establishing rat models of chronic obstructive pulmonary disease (COPD). Methods A total of 24 8-week-old healthy Wistar rats were randomly divided into 2 model groups and a control group (Group C). The rat COPD models were established by two ways; intratracheal injection of lipopolysaccharide (LPS) twice + exposure to cigarette smoke for 1 month (Group A), and cigarette smoke inhalation for 80 days only (Group B). The pathologic characteristics of animal models, including the mean lining interval (MLI) and the mean alveoli number(MAN), were determined. The total and different white blood cell counts in bronchoalveolar lavage fluid(BALF) and blood samples were determined. Results The rats in the two model groups presented with cough or breathlessness periodically, and the white blood cell counts and neutrophil counts in the peripheral blood and BALF were significantly higher than those in the control group (P<0.01). H-E staining showed that the lung tissues of rats in Group A and B had typical pathological features of COPD and emphysema. MLI were significantly higher and MAN were significantly lower in Group A and B than those in group C (both P<0.01), but there was no statistical difference between the two model groups. Group A had more severe inflammatory response in the bronchial and lung tissues than Group B. And Group B was characterized by alveolar overdistension. Conclusion Both the two methods can successfully establish rat COPD model, with its pathophysiological changes similar to those of human COPD, with intra-tracheal injection of lipopolysaccharide being more consistent to the natural development of disease.

Objective To explore the teaching method to improve nursing student's clinical practicing skills. Methods 111 nursing students were divided into observation group (55 cases) and control group (56 cases). The students in observation group were received clinical nursing whole range simulation experiment, while those in control group were received routine method. Results The students in observation group showed high evaluation on experimental results, their scores in final theory exam were higher than that in control group. The teachers showed higher satisfaction for students in ohaservation group. Whereas there were no difference in terms of nursing technique exam between groups. Conclusions Clinical nursing whole range simulation experiment is helpful to shorten the difference between teaching and clinics, make students accommodate the clinical nursing role quickly, and improve their clinical practicing skills.

Objective:To investigate the influence of clinical outcomes and the risk factors of poor prognosis for preoperative nutritional risk in gastric cancer patients.Methods:A prospective study was performed in 140 patients with gastric cancer and the nutritional risk screening 2002 (NRS 2002) was done.The influence of preoperative nutritional risk on postoperative complications,hospital stay,ICU stay,hospital expenses,60 days readmission and mortality was analyzed,and the risk factors of perioperative complication were identified by univariate and multivariate analysis.Results:The significant difference (NRS 2002 ≥ 3 group vs.NRS 2002 ＜ 3 group) was observed in the rates of overall postoperative complications,pulmonary infection,overall hospital stay and postoperative hospital stay (P ＜ 0.05).The rates of anastomotic fistula,pleural effusion,60 days readmission,60 days mortality and hospitalization expenses in NRS 2002 ≥ 3 group were higher than that of NRS 2002 ＜ 3 group,but there were no differences between the two groups (P ＞ 0.05).By univariate and multivariate analysis,preoperative high cholesterol levels and preoperative nutritional risk are the risk factors of poor prognosis and postoperative complications.Conclusion:Preoperative high cholesterol levels and preoperative nutritional risk are independent risk factors of postoperative complications.

Objective To analyze the plasma procalcitonin (PCT) as a predictor of the severity of community acquired pneumonia (CAP) in elderly patients.Methods Totally 90 elderly patients hospitalized with community acquired pneumonia from 2010 to 2011 were analyzed retrospectively for the relation between plasma procalcitonin and severity of pneumonia.All cases were divided into two groups,the severe group (n=36) and the non-severe group (n=54) according to diagnostic criteria.Results The level of plasma PCT was much lower in the severe group (median 2.44 μg/L) than that in the non-severe group (median 0.11 μg/L) (U=335.50,P=0.000).Among all patients,when PCT was lower than 0.5 μg/L,the incidence of non-severe CAP was 76％,however,when PCT was equal or above 2.0 μg/L,the incidence of non-severe CAP was reduced to 9％.In Binary logistic regression analysis,PCT was a risk factor of aged person with severe community acquired pneumonia independent of age and CRUB-65 scores [OR =1.328 (95 ％ confidential interval:1.072,1.645)].PCT had a positive correlation with CRUB-65 scores (U=10.162,P=0.006).In all cases,the patients who improved well had lower PCT value than the remaining (median 0.21 μg/L,17.0μg/L; U=10.000,P=0.000),which also happened in severe cases (median 1.47 μg/L,17.0 μg/L;U=8.000,P=0.000).The area under the receiver operating characteristic curve was 0.872 (95％ confidential interval:0.741,0.914).At a PCT cut-off level of greater than or equal to 2.0 μg/L,the sensitivity and specificity to predict the severity of aged person with CAP was 55.6％ and 98.9％ respectively.Conclusions Plasma PCT may be a good predictor to evaluate the severity of CAP in elderly patients.

Objective To explore the efficacy of trazodone replacement treatment on benzodiazepine drugs dependence and effect of cognitive function on senile patients.Methods 51 senile patients with benzodiazepine drugs dependence were assigned with dosage tacho-decrement and replaced by trazodone.The patients were discontinuanced taking benzodiazepine in 14 days and taken at a draught of trazodone before retiring about 6 months.Clinical effect and side effects were assessed with the Pittsburgh sleep quality index (PSQJ) and treatment emergent symptom scale(TESS) before and after treatment.Cognitive function was evaluated with Wechsler intelligence scale for adult-Chinese revised (WAIS-RC) and Wechsler mermory scale for adult-Chinese revised ( WMS-RC ) once before and after treatment.Results The scores total PSQJ( ( 13.17 ± 3.70),( 11.05 ± 3.48 ) ),the sleep quality( (2.36 ± 0.33 ),( 1.91 ± 0.29 ) ),daily function disorder,sleep disorder were significantly lower than before treatment while the other factor scores were not significantly changed.Trazodone wes effective without severe side effects and dependence.The study group showed significantly lower scores in learning,calculation,the signs of figure,wood puzzles,long-term memory,short-term memory,immediate memory,memory quotient in the assessment of cognitive function than after treatmemt (P ＜ 0.05 ).Conclusion Trazodone is an ideal medicine to senile insomnia.

ObjectiveTo determine optimal positive end expiratory pressure (PEEP) in mechanical ventilation in children with severe asthmatoid disease based on the quasistatic pressure-volume (P-V) curve.MethodsA serf-control study was done on 23 children with severe asthmatoid disease in the pediatric intensive care unit( PICU ).Quasistatic lung P-V curve of these patients was analyzed and the lower inflection point (LIP) from P-V curve was determined.Three different PEEP (0 cm H2O,LIP,LIP+2 cm H2O,1 mm H2O =0.098 kPa) were given to the patients.The effects of PEEP at different levels on gas exchange,hemodynamic and airway pressure were observed.ResultsThe quasistatic LIP were (2.70 ±2.00)cm H2O.When PEEP was increased to the level of LIP + 2 cm H2O,PaO2 / FiO2 and lung compliance improved significantly (P ＜ 0.01 ) and dynamic lung compliance was the highest,peak inspiratory pressure was (22.30 ± 3.00) cm H2O and mean airway pressure was( 14.11 ± 1.01 ) cm H2O,without obvious adverse effects on mean arterial blood pressure and heart rate.There was no difference in PaCO2,when compared PEEP =0 cmH2O to PEEP =LIP + 2 cmH2O.ConclusionThe application of PEEP is safe.LIP + 2 cm H2O from quasistatic P-V curve could be set as the optimal PEEP under which mechanical ventilation has the best efficacy and do not aggravate CO2 retention and abnormality of hemodynamics in children with severe asthmatoid disease.

Child, Preschool ; Female ; Humans ; Infant ; Male ; Methionine ; blood ; Tyrosine ; blood ; Tyrosine Transaminase ; deficiency ; Tyrosinemias ; blood ; diagnosis ; enzymology ; pathology ; therapy