Xanthotoxol (XT) is a coumarin isolated from ethanolic extract of fruits of Cnidium monnieri (L. )Cus　son. It is effective not only for the prevention of ventricular fibrillation of mice induced by chloroform or that of rats induced by calcium chloride,but also is effective for the treatment of arrhythmia induced by aconitine in rats. It can raise the threshold of ventricular fibrillation induced by electrical stimulation in rabbits. Fur　thermore,XT can inhibit the action potential amplitude of isolated sciatic nerves in toads. These results indi　cated that XT possesses antiarrhythmic effects. The iv LD50 of XT in mice is 47.0mg/kg

Objective:To explore the effect of early exercise rehabilitation in patients with acute myocardial infarction (AMI).Methods:A total of 64 AMI patients with stable vital signs were randomly divided into early exercise reha-bilitation group (n=32)and routine rehabilitation group (n=32)according to number table method.Early exercise rehabilitation group received early exercise rehabilitation guidance by rehabilitation therapists on first day after on-set,while routine rehabilitation group received exercise rehabilitation guidance by rehabilitation therapists of same qualification after one-week absolute bed rest.Besides,the two groups received same therapy and nursing.Incidence of major adverse cardiovascular events (MACE:re-infarction,death,severe arrhythmias,angina pectoris after in-farction and heart failure),hospitalization days and condktion of life self-care ability were observed,compared and analyzed between two groups.Results:Before discharge,there were no significant difference in MACE incidence rate (40.63% vs.43.75%)and percentages of patients with life self-care ability (100% vs.96.88%)between early exercise rehabilitation group and routine rehabilitation group,P >0.05,but length of hospital stay in early rehabili-tation group [(9.23±1.45)d]was significantly shorter than that of routine rehabilitation group [(15.03±2.53) d],P <0.01. Conclusion:Early exercise rehabilitation training can shorten length of hospital stay and is safe in pa-tients with acute myocardial infarction.

Objective:To explore therapeutic effect of pharyngeal treatment machine (PTM) combined resuscitating acupuncture (RA) on patients with dysphagia after stroke.Methods: A total of 98 stroke patients with dysphagia were selected.According to random number table, they were randomly and equally divided into pure pharyngeal treatment group (received pure PTM therapy based on routine treatment) and combined treatment group (received RA based on pure pharyngeal treatment group).Video fluorography (VFG) score and swallow-quality of life (SWAL-QOL) score before and after treatment, and total effective rate were evaluated and compared between two groups.Results: Compared with before treatment, there was significant rise in VFG score, and significant reduction in SWAL-QOL score in both groups after treatment, P<0.01 all;compared with pure pharyngeal treatment group after treatment, there was significant rise in VFG score [(5.46±2.04) scores vs.(6.54±2.75) scores], and significant reduction in SWAL-QOL score [(741.33±25.92) scores vs.(632.54±26.77) scores] in combined treatment group, P=0.032, 0.001 respectively.Total effective rate of combined treatment group was significantly higher than that of pure pharyngeal treatment group (93.88% vs.75.51%), P=0.014.Conclusion: Compared with pure pharyngeal treatment machine, pharyngeal treatment machine combined resuscitating acupuncture possesses better therapeutic effect for recovery of swallowing function, which is worth extending.

ve To understand the impacts of industrial fluoride-induced pollution on environment and human health. Methods The contents of fluorides in environmental media, such as air, soil, vegetables, weeds, branches and leaves in area around a certain chemical factory (polluted area) were monitored. The prevalance of dental fluorosis, the contents of fluorides in hair and nail were investigated among 87 individuals without history of exposure to industrial fluorides, living in polluted area for more than 5 years, and 132 individuals in control area. Results Higher fluoride levels in environmental medias, higher prevalance rates of dental fluorosis, high contents of fluorides in hair and nails of population were found in polluted area compared with those in control area. Conclusion The in-dustrial fluoride-induced pollution from this chemical factory had resulted in the hazardous impacts on environmental quality and human health.

Objective To observe the therapeutic efficacy of low-frequency electrical stimulation (LFES) plus acupuncture in treating deglutition disorders after cerebral stroke. Method Seventy-six patients were randomized into two groups. The treatment group (38 cases) was intervened by LFES plus acupuncture;the control group (38 cases) was by LFES. Result The water drinking test scores were significantly changed after the treatment in both groups (P<0.05); after the intervention, there were significant differences in comparing the water drinking test score, improvement score, and therapeutic efficacy grading between the two groups (P<0.05). Conclusion LFES and acupuncture are both effective in treating deglutition disorders after cerebral stroke, and the combination of the two methods can enhance the clinical efficacy.

OBJECTIVETo explore the impact and effect mechanism of electroacupuncture (EA) on oocyte quali ty in the patients with infertility of kidney deficiency pattern.

METHODSSixty-six cases differentiated as kidney de ficiency and with in vitro fertilization-embryo transplantation (IVF-ET), aged fromnt 35 to 42 years were rando- mized into an observation group and a control group, 33 cases in each one. The IVF-ET therapy of the long proto- col with gonadotrophin releasing hormone agonist was adopted in the two groups. In the observation group. on the 5th day of menstruation in IVF cycle, EA was applied to Sanyinjiao (SP 6). Zigong (EX CA 1), Zhongji (CV 3) and Guanyuan (CV 4). In the control group, the sham-acupuncture was applied to the same acupoints. The treatment was given once every two days till the date of egg collection and the needles were retained for 30 min each time. The change in the score of kidney deficiency syndrome, the high-quality oocyte rate, the high-quality embryo rate and clinical pregnant rate were observed in the two groups. The levels of insulin-like growth factor-i (IGF-1) and IGF-2 in follicular fluid and the serum β-endorphin β-EP) on the date of egg collection and the correlation with oocyte quality were compared bIetween the two groups.

RESULTS1) In the observation group, the kidney deficiency syndrome score after treatment was reduced apparently as compared with that before treatment (P<0. 05), the score after treatment in the observation group was reduced much more apparently as compared with the control group (P<0.05). 2) The high-quality egg rate and the high-quality embryo rate in the observation group were both higher than those in the control group [81.3% (161/198) vs 57.6% (98/170), 59.8% (58/97) vs 37.7% (26/69), both P<0.05]. 3) Compared with the control group. the levels of IGF-1 and IGF-2 in follicular fluid and serum β-EP on the day of egg collection were all increased obviously in the observation group (all P<0. 05). 4) The levels of IGF-1 and IGF-2 in follicular fluid and serum β-EP presented the linear positive correlation with the high-quality egg rate.

CONCLUSIONEA effectively improves the expressions of IGF in follicular fluid and serum β-EP, increases the high-quality egg rate and high-quality embryo rate and relieves the symptoms of kidney deficiency.

Acupuncture Points ; Adult ; Electroacupuncture ; Female ; Fertilization in Vitro ; Humans ; Infertility, Female ; metabolism ; physiopathology ; therapy ; Insulin-Like Growth Factor I ; metabolism ; Kidney ; physiopathology ; Oocytes ; Pregnancy ; beta-Endorphin ; metabolism

Actins ; metabolism ; Adult ; Breast Diseases ; metabolism ; pathology ; surgery ; Breast Neoplasms ; pathology ; Calcium-Binding Proteins ; metabolism ; Carcinoma ; pathology ; Diagnosis, Differential ; Fasciitis ; metabolism ; pathology ; surgery ; Female ; Fibroma ; pathology ; Fibrosarcoma ; pathology ; Follow-Up Studies ; Humans ; Microfilament Proteins ; metabolism ; Vimentin ; metabolism ; Young Adult

Objective: To study the anti-influenza A virus effect of theaflavin derivatives which contain theaflavin (TF1), theaflavin-3-gallate (TF2A), theaflavin-3'-gallate (TF2B), and theaflavin-3, 3'-digallate (TF3) and to investigate the mechanism. Methods: The inhibition of theaflavin derivatives on A/Thailand/Kan353/2004 H5N1 pseudovirus was investigated using pseudotype H5N1 virus system. Hemagglutination inhibition assay and neuraminidase (NA) inhibition assay were used to investigate the mechanism for their anti-influenza activities. The inhibition of theaflavin derivatives on influenza A virus FM_1 was observed by H1N1 FM_1 system. Cytotoxicity of theaflavin derivatives on MDCK cells was determined by MTT assay. Results: Theaflavin derivatives could significantly inhibit the infection of pseudovirus H5N1 with IC50 of (151.88 ± 18.95) μg/mL. It had no inhibition on HA1 subunit. Theaflavin derivatives had the inhibition on NA with IC50 of (129.09 ± 1.33) μg/mL. Theaflavin derivatives showed a significant inhibitory activity on FM_1 influenza virus strain in MDCK cells. Theaflavin derivatives showed the low cytotoxicity on MDCK cells with CC50 of (879.89 ± 4.54) μg/mL. Conclusion: Theaflavin derivatives could inhibit the infection of avian influenza virus through the combination with hemagglutinin (HA) HA2 subunit and inhibit the activity of viral NA to some extent, which indicates that the anti-H5N1 avian influenza virus of theaflavin derivatives may be through multi-targets.

The extraction method and structure of exo-polysoccharide from schizophyllum commune by submerged cultivation were studied. Proteins were removed com p letely from the polysoccharide by the sevag method following the isoelectric poi nt precipitation method. The purified schizophyllan was proved to be homogeneou s with molecular weight 4?10 4D by sephadex G-200 column chromatography, PAGE and HPLC. Its monomer was determined by hydrolysis, PC, GC and its structure wa s analyzed by IR, enzymolysis, periodate oxidation, the results showed that schi zophyllan was only composed of glucose and it was the ?-glucan consisting of ?-(1-3) and ?-(1-6) glucosidic linkages.

Bardet-Biedl Syndrome ; diagnosis ; therapy ; Child ; Female ; Humans