Acupuncture Therapy ; Child ; Humans ; Male ; Torticollis ; complications ; therapy ; Tourette Syndrome ; complications ; therapy

Embryonic stem cells(ESCs),a kind of totipotent cells,can differentiate into cell type of the 3 germ layers.Adult stem cells(ASCs) are pluripotent stem cells,which can self-renew and transdifferentiate into a completely different tissue derivative cells.Thus ASCs can transdifferentiate into germ cells in a certain condition.Great progress has made for the study on human ESCs.However,use of ESCs faces the immunological rejection,oncogenicity and ethical controversies problems,and these limit their uses for treatment of human diseases.On contrast,ASCs can advoid these problems,and they may have a better perspective.Germ cells as carriers of genetic materials,are worth of studying.If male germ cells can be derived from ASCs,this will open the possibilities for the use of these cells for male infertillty in clinical application.In this review,we will introduce the progress on derivation of male germ cells from ESCs and ASCs.

Aged ; Female ; Humans ; Male ; Middle Aged ; Silicosis

Objective To investigate the clinical significance between congestive heart failure (CHF) and serum thyroxine. Methods 50 patients with CHF (7 cases of grade Ⅱ heart function,grade Ⅲ 19 cases,grade Ⅳ 24)and 30 normal persons wre selected. The serum triiodothyronine( T3 ), four-triiodothyronine( T4 ), free triiodothyronine (FT3), free triiodothyronine (FT4), thyroid stimulating hormone TSH were determined by radioimmunoassay. Results The T3, FT3 contens ( 1.95 ± 0.55) nmol/L, (2.20 ± 0.39 ) pmol/L in CHF group were lower than the control group (2.4 ± 0.50) nmol/L, ( 5.89 ± 0. 45 ) pmol/L ( t = 2. 415,2. 325, P < 0.05 ); The T3, FT3 contens ( 2.19 ± 0. 53 )nmol/L,(3.69 ± 0.57)pmol/L after treatment were significantly higher than that before treatment (t =2. 375,2. 405, P < 0.05 ); The T3, FT3 contens in cardiac function with stage Ⅳ ( 1.76 ± 0.7) nmol/L, ( 1.99 ± 0. 45 ) pmol/L were significantly lower than the control group( t = 2.245,2. 375, P < 0.05 ); Serum TT3, TT4 and cardiac function was negatively correlated ( r = -0.933, -0.383, all P < 0.05 ) , serum FT3, FT4,TSH and cardiac function were not correlated. Conclusion The changes of serum thyroid level could be used as an index of the severity of heart failure.

Celastrol is a type of quinone methyl triterpene isolated from traditional Chinese medicine Tripterygium wilfordii, with pharmacological activities, like anti-inflammatory, immunosuppression and anti-tumor. This study focused on the effects of celastrol on adhesion, migration and invasion of lung cancer cells. The migration inhibition of lung cancer cells induced by celastrol was detected by the scratch test. The invasion inhibition of lung cancer cells induced by celastrol was measured by the transwell experiment. RT-PCR and Western blot were used to determine the effect of different concentrations of celastrol in integrin family and Akt signaling pathway in lung cancer cells. The results showed that celastrol inhibited adhesion, migration and invasion of lung cancer cells and expressions of integrins β3, β4, αv and phosphorylated Akt, GSK-3β, c-Raf, PDK1 in Akt signal pathway in a dose-dependent manner. Therefore, the study implies that Celastrol could inhibit the metastasis of lung cancer cells by suppressing Akt signaling pathway and expression of integrins.
Apoptosis ; drug effects ; Cell Line, Tumor ; Drugs, Chinese Herbal ; pharmacology ; Gene Expression Regulation, Neoplastic ; drug effects ; Humans ; Integrins ; genetics ; metabolism ; Lung Neoplasms ; genetics ; metabolism ; pathology ; physiopathology ; Neoplasm Metastasis ; Proto-Oncogene Proteins c-akt ; genetics ; metabolism ; Signal Transduction ; drug effects ; Triterpenes ; pharmacology

Objective To investigate the application and nursing care of bronchoalveolar lavage (BAL)by fiberoptic bronchoscopy in patients with mechanical ventilation. Methods 64 patients with mechanical ventilation in RICU were randomly divided into the control group and the observation group with 32 patients in each group. The patients in both groups were accepted anti-infection, atomization inhalation and other supportive treatments. The patients in the observation group were treated with BAL, and the patients in the control group accepted routine treatment. The therapeutic effects in the two groups were evaluated and compared. Results There was significant difference in the recovery rate and effective rate, the blood gas analysis compared with the control group was significantly improved, the time of pulmonary infection control window, mechanical ventilation, length of stay were shorter than the control group. The case number to remove ventilator successfully, organs failure and hospital mortality were less than the control group. Conclusions Fiberoptic bronchoscopy with BAL treatment could be safety and efficacy for patients with mechanical ventilation. Nursing intervention plays an important role and a good therapeutic effect for improving achievement ratio of BAL.

Objective To study the effects of individualized nursing on the anxiety, depression mood, quality of life of patients with hemoptysis. Methods Sixty patients with hemoptysis were randomized into the intervention group and the control group with 25 cases in each. The control group received single conventional therapy while the intervention group was given individualized nursing intervention besides the conventional therapy. Using SDS, SAS and SF-36 Life Quality Measuring Scale to evaluate the effects of the patients in both groups on admission and prior to discharge. Results The scores of anxiety and depression among the intervention group were lower than those of the control group after intervention.The dimensions of quality of life besides the physical function and bodily pain in the intervention group were significantly different after intervention. Conclusions The individualized nursing intervention can improve quality of care, the state of anxiety, depression and the quality of life of patients with hemoptysis.

Objective To discuss the effect of health education to family members of patients with chronic heart failure. Methods 150 patients with family members as accompanies were divided into the observation group and the control group with 75 patients in each group. Health education was given to patients as well as their family members in the observation group, while health education was given only to the patients in the control group. The mastering of disease-related knowledge by family members of the observation group was observed after 15 days. The rehospitalization of the two groups by telephone follow-up was carried out six months after discharge. Results The mastering of disease-related knowledge by family members of the observation group was better compared with before health education. The rehospitalization rate of the observation group was 13.3%, which was lower than 44.0% of the control group. Conclusions Health education to family members of patients with chronic heart failure can increase treatment effect of patients, decrease disease relapse and lower rehospitalization rate.

The painless needle insertion technique, summarized by Professor WU Lian-zhong during his decades of acupuncture clinical practice is introduced in this article, which is characterized as soft, flexible, fast, plucking and activating antipathogenic qi. The Sancai (three layers) lifting and thrusting manipulation technique is adopted by Professor WU for getting the qi sensation. And features of 10 kinds of needling sensation such as soreness, numbness, heaviness, distension, pain, cold, hot, radiation, jumping and contracture are summarized. Finger force, amplitude, speed and time length are also taken as the basis of reinforcing and reducing manipulations. Moreover, examples are also given to explain the needling technique on some specific points which further embodies Professor WU's unique experiences and understandings on acupuncture.
Acupuncture Therapy ; history ; methods ; China ; History, 20th Century ; History, 21st Century ; Humans ; Needles

In this paper, we present a 3D virtual phantom design software, which was developed based on object-oriented programming methodology and dedicated to medical physics research. This software was named Magical Phan tom (MPhantom), which is composed of 3D visual builder module and virtual CT scanner. The users can conveniently construct any complex 3D phantom, and then export the phantom as DICOM 3.0 CT images. MPhantom is a user-friendly and powerful software for 3D phantom configuration, and has passed the real scene's application test. MPhantom will accelerate the Monte Carlo simulation for dose calculation in radiation therapy and X ray imaging reconstruction algorithm research.
Algorithms ; Humans ; Image Processing, Computer-Assisted ; Monte Carlo Method ; Phantoms, Imaging ; Software ; Software Design