Objective: To investigate the proliferation of three subtypes of odontogenic keratocysts(OKC), namely simple(not recurrent),recurrent and basal cell naevus syndrome(BCNS) associated lesions. Methods: Ki 67 expression was studied in 34 odontogenic keratocysts(simple, n =10;recurrent, n =12;syndrome, n =12)by biotin streptavidin method using Ki 67 monoclonal antibody after microwave treatment. Ki 67 positive cells were counted manually and related to the area of epithelial lining as determined by computer image analyzer. Results: Ki 67 positive cells per mm 2 in simple odontogenic keratocysts ①, recurrent odontogenic keratocysts ② and BCNS ③ were 1 812.29?606.47, 2 393.88 ?997.08 and 3 983.17?858.92 respectively(① or ② vs ③ P 0.05). Conclusion: The basal cell naevus syndrome associated OKC has a higher rate of epithelial proliferation than non syndrome OKC, the increased epithelial proliferation is correlated with increased recurrence potential. Ki 67 may be an alternative method to differentiate syndrome and non syndrome OKC.

Objective To evaluate the long term efficacy of radical cystectomy and ileal conduit in the treatment of bladder cancer. Methods 196 cases of bladder tumor treated from Jan. 1985 to Jan. 2000 with radical cystectomy and ileal conduit for urinary diversion were studied retrospectively. Results Bladder neck or trigone was involved by the neogrowth in 126(64.3%).The growth has been invasive in 145(74.0%). 93.4%(183/196) of the tumor were transitional cell carcinoma. Early operative complication occurred in 19(9.7%) and late complication in 10(7.4%).On following up for 1 to 15 years with a mean of 6.6 years,the 5 year survival rate has been 66.2%. Conclusions Radical cystectomy is the optimal in treating invasive bladder tumor and ileal conduit is a good means of urinary diversion which is simple with minimal complications.

Objective To evaluate transureteroscopic pneumatic lithotripsy in the treatment of ureteral calculi. Methods 116 cases of ureteral calculi treated with ureteroscopic pneumatic lithotripsy from Dec.1999 to Feb.2002 were studied retrospectively.There were 49 male patietns and 67 female with an average age of 43,3 being upper ureteral stone,24 middle and 68 lower ureteral stone.21 ureteral stone-street. Results The stone fragmentation rate was 90%(104/116),85%(99/116) of which being fragmented on the first treatment session.The main complications were failure to insert the ureteroscope in 3 cases and ureteral perforation in 4.94 cases were follow-up for one to eighteen months and 91 cases were stone free within one month.No ureteral stricture has been observed on intravenous urogram. Conclusions Treatment of ureteral calculi by transureteroscopic pneumatic lithotripsy has been safe, effective and less invasive.

Diabetic Neuropathies ; blood ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Nitric Oxide ; blood ; Phytotherapy

Acute myocardial infarction (AMI) is caused by acute occlusion of the coronary artery. The partial necrosis of the myocardium is caused by severe and prolonged ischemia,which is characterized by persistent and severe chest pain, the high mortality rate. Fast recovery can be achieved the coronary artery blood circulation by emergency percutaneous coronary intervention (PCI), can improve myocardial is-chemia, save moribund myocardium. The medical profession is generally believed that on the basis of en-suring the safety of patients with AMI, the normal rehabilitation should be carried out as soon as possible. In this article, the domestic in recent years, AMI emergency PCI in early postoperative rehabilitation treatment method, the intensity and evaluation index of three aspects were summarized. The starting time of the early rehabilitation of AMI patients in foreign countries is earlier than our country. As we all know that via radial artery PCI has the smaller puncture and more convenient hemostasis, so there is no doubt that it can pro-vide favorable conditions for recovery of patients. Therefore, it is necessary to expand the research field in order to determine the best rehabilitation opportunity and the most excellent rehabilitation program , so as so determine the best recovery time and the most excellent rehabilitation program in patients with AMI after PCI operation.

Objective To investigate the clinical and angiographic features in hemorrhagic moyamoya disease patients with the posterior circulation involvement (PCI). Methods The clinical and imaging data of 224 patients with hemorrhagic moyamoya disease from December 2002 to December 2011 were analyzed retrospectively. The patients were divided into either a PCI group (n=57)or a non-PCI group (n=167) according to whether they had PCI or not. Results (1)Suzuki staging concentrated in stageⅤandⅥin the PCI group,accounting for 42. 1%(24/57)and 40. 4%(23/57)respectively;Suzuki staging concentrated in stage Ⅲ and Ⅳ in the non-PCI group,accounting for 31. 1%(52/167)and 41. 9%(70/167)respectively. There was significant difference in the distribution of Suzuki staging between the patients of both groups (P <0. 01). (2)In the PCI group,the thalamic hemorrhage,intraventricular hemorrhage,ganglia hemorrhage,subarachnoid hemorrhage accounted for 43. 9%(26/57),38. 6%(22/57), 3. 5%(2/57),14. 0%(8/57),respectively;There was no cerebral lobe hemorrhage. In the non-PCI group, the thalamic hemorrhage,intraventricular hemorrhage,basal ganglia hemorrhage,subarachnoid hemorrhage and cerebral lobe hemorrhage accounted for 0,37. 7%(63/167),25. 1%(42/167),15. 6%(26/167),21.6 (36/167),respectively. The proportion of hemorrhage types between the PCI group and non-PCI group was significantly different(P<0. 01). (3)The dilation of anterior,posterior choroidal artery ,and patency of posterior communicating artery,posterior pericallosal artery in bleeding side accounting for 19. 3%(11/57),82. 5%(47/57),36. 8%(21/57),78. 9%(45/57)in PCI group and 36. 5%(61/167),3. 0%(5/167),68. 9%(115/167),80. 2%(134/167)in non-PCI group. The ratio of of abnormal vessels between PCI group and non-PCI group had significantly statistical difference (P<0. 01). Conclusion Hemorrhagic moyamoya with PCI is common. Suzuki staging is usually in the advanced stage of vascular staging of moyamoya disease. The thalamic hemorrhage is the main type of bleeding. Posterior choroidal artery rupture may be the main reason of hemorrhage.

Objective To analyze the corneal flap thickness in laser in situ keratomileusis(LASIK) using Moria M2 microkeratome and to identify the related factors. Methods Sixty patients with LASIK were divided into two groups: M2 90 group,using the Moria M2 90 microkeratome,n=30;M2 110 group,using the Moria M2 110 microkeratome,n=30.All were performed on both eyes with the right one treated first.Subtraction pachymetry was used to measure corneal flap thickness which was analyzed statistically with the data including age,preoperative corneal diameter,curvature,corneal thickness and refraction. Results In the 30 patients of M2 90 group,the mean corneal flap thickness of right eye and left eyes were(128.03?12.03)?m(105~156 ?m) and(123.40?12.38) ?m(92~147 ?m),respectively,and the corneal flap thickness were statistically different between the right and left eyes(P

Insect viruses are attractive as biological control agents and could be a feasible alternative to chemical insecticides in the management of insect infestations. This review describes recent advances in the development of wild-type and genetically modified viruses as insecticides. A new strategy of application of insect viruses in China is reviewed. Also, the assessment of biosafety of genetically modified Helicoverpa armigera Nucleopolyhedovirus (HearNPV) is emphasized as a case-study.

A sectional survey with histochemical technique was carried out on Culex tritae-niorhynchus larvae infected with Coelomomyces indica in comparison to the noninfected larvae. Studies were pursued by using micrograph and imaging analysis. The results showed that the glycogen, protein and nucleic acid (RNA and DNA)reaction in the infected group were less than those of the control group. The gray level assessment in tissue imaging showed marked difference between the two groups. It is suggested that C. indica has significant effect on the above biochemical elements of the mosquito larvae, which might be considered an important mechanism in the pathogenicity of the fungus.

Objective To evaluate of the glucose screening retest for gestational diabetes mellitus (GDM) during pregnancy. Methods A retrospective analysis of 714 pregnant women screened for GDM, between December 1,2001, and December 31,2002, was performed.The first glucose challenge test(GCT) was performed in 16～27 week and retested in 28～38 week.Diagnosis of GDM was based on the criteria of Dong.NDDG criteria was also discussed. Results (1) 1-hour glucose value of 50 g GCT ≥7.8 mmol/L was set as abnormal.The first 50 g GCT abnormal rate was 26.6%(190/714),and the retest abnormal rate was 35.2%(225/639).The mean age of pregnant women in 50 g GCT positive group was significantly higher than that in the negative group( P