Objective To investigate the bacterial spectrum changes and characteristics of drug resistance of patients with severe acute pancreatitis (SAP) and to guide clinical proper use of antibiotics medicine .Methods Clinical specimens of 50 patients with SAP were analyzed including sputum ,blood , urine ,central venous catheter ,bile ,et al .Bacterial strains were regularly isolated and drug sensitivity test was made by disc diffusion method .Chi-square test was performed for statistical analysis .Results One hundred and fifty-six bacterial strains were isolated .The number of strains isolated from the sputum , blood ,pancreas and abdominal cavity ,bile ,urine tract ,surgical incision ,oral secretion was 51 ,37 ,24 , 23 ,11 , 8 and 2 , respectively . The most common bacterial strains were Acinetobacter baumannii , Escherichia coli ,Pseudomonas aeruginosa and Enterococcus f aecium ,the number of strains was 30 ,21 , 20 and 14 ,respectively .The drug resistance rate of Acinetobacter baumannii to imipenem and meropenem was no less than 90% .The drug resistance rate to cefoperazone/sulbactam was lower ,but still over 60% . The drug resistance rate of Escherichia coli to penicillins ,majority of cephalosporins and quinolones was over 90% . The drug resistance rate of Pseudomonas aeruginosa to partial cephalosporins was high (90% ) ,and the drug resistance rate to imipenem was also up to 65% . T he drug resistance rate of Enterococcus faecium to penicillin G and quinolones was up to 100% . No Enterococcus resistant to tigecycline ,vancomycin and linezolid was found .The infection rate of patients received invasive operation was higher than that of patients received no invasive operation .Conclusions The main bacteria of patients with SAP complicated with infection was Gram-negative bacteria ,which has high drug resistance .The common locations of infection were respiratory tract ,blood ,abdominal cavity ,biliary system and urinary tract .The infection in respiratory tract and blood may be related with invasive medical operations .

Objective Study the behaviors of image registration algorithms, and analyze the elements which influence the performance of image registrations. Methods Pre-known corresponding coordinates were appointed for reference image and moving image, and then the influence of region of interest (ROI) selection, transformation function initial parameters and coupled parameter spaces on registration results were studied with a software platform developed in home. Results Region of interest selection had a manifest influence on registration performance. An improperly chosen ROI resulted in a bad registration. Transformation function initial parameters selection based on pre-known information could improve the accuracy of image registration. Coupled parameter spaces would enhance the dependence of image registration algorithm on ROI selection. Conclusions It is necessary for clinic IGRT to obtain a ROI selection strategy (depending on specific commercial software) correlated to tumor sites. Three suggestions for image registration technique developers are automatic selection of the initial parameters of transformation function based on pre-known information, developing specific image registration algorithm for specific image feature, and assembling real-time image registration algorithms according to tumor sites selected by software user.

Aged ; Female ; Humans ; Male ; Middle Aged ; Silicosis

BACKGROUND:Ankylosing spondylitis is an autoimmune disease involved in chronic systemic inflammation. Tumor necrosis factor and interleukin-6 levels increased in patients with ankylosing spondylitis. Inflammatory factors such as tumor necrosis factor and interleukin-6 can suppress CD36 expression in monocytes. OBJECTIVE: To analyze the correlation between CD36 expression in monocytes and ankylosing spondylitis. METHODS:A total of 84 newly diagnosed ankylosing spondylitis patients and 111 healthy individuals were included in this study. CD36 expressions in monocytes in ankylosing spondylitis patients and healthy individuals were tested using flow cytometer; meanwhile, biochemistry, immunology, routine blood examination and related inflammatory markers were determined between the two groups. RESULTS AND CONCLUSION:Results of baseline data in both groups demonstrated that CD36 fluorescence intensity in monocytes was significantly lower in patients with ankylosing spondylitis compared with healthy controls (P < 0.01). CD36 fluorescence intensity in monocytes was negatively correlated with C-reactive protein, erythrocyte sedimentation, interleukin-6 and tumor necrosis factor. In addition, CD36 fluorescence intensity in monocytes was negatively correlated with BASDAI score. Logistic regression analysis showed that erythrocyte sedimentation, interleukin-6, tumor necrosis factor and CD36 fluorescence intensity in monocytes were associated with ankylosing spondylitis, and risk factors for ankylosing spondylitis (P < 0.05). These findings confirm that inflammatory cytokine in patients with ankylosing spondylitis weakened the expression of CD36 in monocytes. There was a remarkable association between low expression of CD36 expression in monocytes and ankylosing spondylitis. CD36 expression of monocytes clinicaly may be considered to be an effective indicator to evaluate inflammation and disease activity in patients with ankylosing spondylitis.

Diabetic Neuropathies ; blood ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Nitric Oxide ; blood ; Phytotherapy

Objective:To summarize the allergic mechanism caused by traditional Chinese medicine injection (TCMI) to provide some references for perfecting Good Laboratory Practice(GLP) of TCMI.Method:The related iteratures in data bases at home and abroad were reviewed,and the present experimental research methods about allergies were referred to to have a view of future studies.Result:The allergic mechanism of TCMI was mostly antigen-antibody reaction, part of which was anaphylactoid reaction.The method of the evaluation of allergy caused by TCMI only was animal experiments, but there were still some allergies caused by TCMI after the evaluation with this method.The present experimental research methods indicated that the detection of mediators of inflammation and FCM(Flow Cytometry) could be used to evaluate the allergies caused by TCMI.Conclusion:More attention should be paid to allergies caused by TCMI for its complicated mechanism and frequent occurrences in clinic.It may be an effective way to evaluate the allergies caused by TCMI with several methods including in vivo and in vitro.

Multiplicity of signals and diversity of signaling pathways exist during the establishment of mycorrhizal associations together with the regulation of symbiosis-specific genes expression.This mechanism of signal recognition and transduction related with development process of the symbiont was reviewed at the molecular level.

Child ; China ; Diarrhea ; diagnosis ; microbiology ; Escherichia coli Infections ; diagnosis ; Humans ; Shiga-Toxigenic Escherichia coli ; isolation & purification

Acupuncture Therapy ; Administration, Inhalation ; Aged ; Aged, 80 and over ; Cerebral Infarction ; drug therapy ; therapy ; Combined Modality Therapy ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Male ; Middle Aged ; Oxygen Inhalation Therapy

OBJECTIVETo observe the effect and mechanisim of zoledronate sodium on periprosthetic osteolysis in rat induced by polyethylene particles.

METHODSTotal 30 adult male SD rats, weighting from 250 to 300 g, were selected and randomly divided into three groups: blank control group, model control group and zoledronate sodium group respectively, 10 animals for each group. No treatment was performed in the blank control group. In model control group and zoledronate sodium group, the modle of periprosthetic osteolysis in rats were made by implanting polyethylene particles and titanium rods into their right femurs. After operation, rats in zoledronate sodium group were administered with zoledronate sodium (0.1 mg/kg each week) through subcutaneous injection for 8 weeks, then the blood was obtained and all experimental animals were sacrificed to get the right femur specimens. The femur BMD, IL-1β, IL-6, TNF-α, serum TRACP5b and CTX-I were detected.

RESULTSCompared with the model control group, the femur BMD was increased, while IL-1β, IL-6 and TNF-α were all decreased in zoledronate sodium group; the serum TRACP5b and CTX-I level were both reduced in zoledronate sodium group.

CONCLUSIONThe zoledronate sodium could effectively inhibit periprosthetic osteolysis in rats induced by polyethylene particles, which might be realized by inhibiting the activity of osteoclasts and the expression of IL-1β, IL-6 and TNF-α. It provides a new method to treat periprosthetic osteolysis of the artificial joint prosthesis.

Animals ; Bone Density ; drug effects ; Collagen Type I ; analysis ; Cytokines ; analysis ; Diphosphonates ; therapeutic use ; Imidazoles ; therapeutic use ; Joint Prosthesis ; Male ; Osteolysis ; drug therapy ; Peptides ; analysis ; Polyethylene ; pharmacology ; Rats ; Rats, Sprague-Dawley