In this paper, the variation of surface electricity of two Entamoeba histolytica isolar es was investigated using the cell electrophoretic technique. By testing the electrophoretic iondition of amoeba in gradient pH and the effect of some enzymes, we provided infor-nations for the physiology and pathogenicity of this parasite.Cell electrophoresis for the two isolates of E. histolytica (isolate A and isolate B) n pH 2.05-8.00 Mcllvaine buffer revealed that EPM (electrophoretic mobility) was negative under the pH gradients of 3.00-8.00, which meant the amoeba cell surfacecar- ried negative charge in this condition. Surface charge densities of isolates A and B were found to be ( + ) 148.3-(-) 6035.9 esu/cm2 and (+ )222.4-(-) 6624.0 esu/cm2 respectively. In addition, except in pH 2.05, the EPMs in Mcllvaine buffer were different between the two amoeba isolates, with significant statistical difference.The enzyme test demonstrated there was no neuraminic acid on the surface of these two amoeba isolates. The surface negative groups of the protozoa were sensitive to trypsin and deoxyribonuclease I.

Objective:To explore the effect of early exercise rehabilitation in patients with acute myocardial infarction (AMI).Methods:A total of 64 AMI patients with stable vital signs were randomly divided into early exercise reha-bilitation group (n=32)and routine rehabilitation group (n=32)according to number table method.Early exercise rehabilitation group received early exercise rehabilitation guidance by rehabilitation therapists on first day after on-set,while routine rehabilitation group received exercise rehabilitation guidance by rehabilitation therapists of same qualification after one-week absolute bed rest.Besides,the two groups received same therapy and nursing.Incidence of major adverse cardiovascular events (MACE:re-infarction,death,severe arrhythmias,angina pectoris after in-farction and heart failure),hospitalization days and condktion of life self-care ability were observed,compared and analyzed between two groups.Results:Before discharge,there were no significant difference in MACE incidence rate (40.63% vs.43.75%)and percentages of patients with life self-care ability (100% vs.96.88%)between early exercise rehabilitation group and routine rehabilitation group,P >0.05,but length of hospital stay in early rehabili-tation group [(9.23±1.45)d]was significantly shorter than that of routine rehabilitation group [(15.03±2.53) d],P <0.01. Conclusion:Early exercise rehabilitation training can shorten length of hospital stay and is safe in pa-tients with acute myocardial infarction.

The antischistosomal effects of Levo-praziquantel (L-PZQ) compared with racemic prazi-quantel (dL-PZQ) in vitro on miracidium and cercaria of Schistosoma japonicum were reported in this paper. The results indicated that the schistosomicidal effect on the two larval stages of the parasite induced by L-PZQ (0. 2 ?g/ml) and double amount of dL-PZQ (0. 4?g/ml) had no significant difference. Miracidial stage was more sensitive to the drugs than the cer-carial stage. The schistosomicidal mechanism caused by L-PZQ was discussed briefly.

Objective To investigate the expression pattern of hypoxia inducible factor-1?(HIF-1?) in fetal vertebra development of the mouse. Methods The development of mouse fetal vertebra was observed dynamically,and the expression of HIF-1? mRNA at various stages was also detected by reverse transcription-polymerase chain reaction. Results The cartilaginous spine column began to form at E13.5.The primary ossification center was observed at E15.5,then the osteogenesis expanded and extended to both sides.HIF-1? mRNA began to express at E13.5,and more significantly at E14.5(P

Objective To investigate the protective effects of ulinastatin on express of GRP 78、CHOP and caspase-12, the molecules related to endoplasmic reticulum stress (ERS),after ischemia reperfusion injury in rats.Methods Ninety rats were equally randomized into 3 groups(n=30): Sham group (S group,n=30), Ischemia -reperfusion group (I/R group, n=30), Ulinastatin group (U group, n=30).Focal transient cerebral ischemia model was established with intralu-minal occlusion fo left meddle cerebral artery .Made through 2 hours of temporary middle cerebral artery occlusion , followed with 24 hours of reperfusion .The pathological results were investigated by HE staining and the cerebral injury situation was e -valuated by neurological deficit scores .Infract volume was measured by TTC staining , apoptosis was detected by TdT -medi-ated dUTP and nick end labeling (TUNEL), and expression of GRP78, CHOP and caspase -12 were meastured by western blot.Results Compared with the S group , the number of apoptotic cells were significantly increase in I /R group and U group (P<0.05);infarct volume and expression of GRP -78, CHOP and caspase-12 were significantly increased in I/R group and U group (P<0.05).The infarct volume and the number of apoptotic cells were significantly less in U group than in I/R group ( P<0.05 ) .GRP78 expression was higher in U group than in I/R group ( P<0.05 ) , however CHOP and caspase-12 expression was less in U group than in I/R group (P<0.05).Conclusion Ulinastatin has a protective effect on cerebral ischemia reperfusion injury , which may related to increased GRP 78, decreased CHOP and caspase -12 expres-sion and to inhibition of the ERS -induced apoptosis pathway .

Objective To discuss the method to increase the success rate of stomach tube insertion for patients with indwelling nasotracheal tube in order to shorten the time of insertion operation and reduce the discomfort of patients.Methods Seventy patients with nasotracheal tube were randomly divided into the observation group(38 cases)and the control group(32 cases).When the operator for the observation group inserted stomach tube to 18～22 centimeters deep,the nurse pumped the gas out from the tracheal catheter aerocyst.After the stomach tube reached stomach the tracheal catheter aerocyst was aerified again.The control group did not release the gas from tracheal catheter aerocyst when inserting the stomach tube.The first intubation success rate and time needed for intubation were compared between the two groups.Results The first intubation success rate of the observation group was higher than that of the control group(P＜0.05).While the time needed for intubation was shorter than that of the control group(P＜0.01).Conclusions Method used by the observation group not only increased the first intubation success rate,but also shortened time needed for intubation.This method can alleviated pain of patients and worth applying widely in clinic.

Objective To investigate a simple, valuable new method for coverage of the soft tissue defect over the lower one-third of the leg, ankle and the foot. Methods The distally based peroneus brevis muscle flaps were applied for coverage of the soft tissue defects over the lower one-third of the leg, the ankle and the foot. The muscle flaps were covered with split-thickness skin grafts. Results The distally based peroneus brevis muscle flaps were applied for coverage of the soft tissue defects over the lower one-third of the leg, the ankle and the foot in 16 cases. The largest area of the soft tissue defect was 5 cm?7 cm. The smallest was 3 cm?4 cm. Primary healing occured in 14 cases undergoing muscle flap construction, second-stage healing occured in 2 cases, no total flap necrosis occured in any cases. Conclusion This technigue is a simple and complications are lesser. The successful rates are higher. This muscle flap is suitable to the mudium or small soft tissue defect especially.

In 51 patients with gastrointestinal diseases,diseases,abdondnal X-lay Photos at lying POSition weretaken 6.5 hours after barium ined and 12 healthy volunteers served as coneal. The longitudinal axis,the transverse diameter,the number and the height of colonic bags in the colonic X-ray photes at fillingand evacuating Phases were counted.Chronic colitis was mainly manifested as loose stool, alternation ofconstipation and diarrhea,dyschesia or abdominal pain whenever desire to defecate. The colonic X-rayphotos showed that the diameter and the number of colonic bag in chronic colitis were reduced and the evacuation of barium became accelerated.These colonic X-ray images can explain the pathophysiologicmechasm of the above manifestations and may serve as a kinetic index providing evidence for the clinicaldiagnosis and treatment of the disease.

Objective To explore the expression of SP-A in surface active substance of sputum pulmonary of dysfunctional venti-latory weaning response patients and to study changes of expression of SP-A and oxygenation index ,arterial partial pressure of oxy-gen .Methods The mechanical ventilatory patients were divide into dysfunctional ventilatory weaning response group (group A) and easily weaning group(group B) ,then collected sputum from ventilators at different times :on the first day ,two and seven days after using ventilators .Used ELISA to test level of SP-A and oxygenation index ,arterial partial pressure of oxygen in sputum of group A and group B .Results For group A ,SP-A content oxygenation index and arterial partial pressure of oxygen were significantly less than those for group B(P<0 .05);after two days ,for group A ,SP-A content in sputum was significantly less than that on the first day ,and SP-A content in sputum was significantly less than that on the second day (P<0 .05);two days later ,oxygenation index and arterial partial pressure of oxygen were significantly lmore than those on the first day (P<0 .05) and there was no statistical difference in oxygenation index and arterial partial pressure of oxygen between the seventh day and the second day (P>0 .05) .Con-clusion Dysfunctional ventilatory weaning response and lung injury related to ventilator can be diagnosed early through the test of SP-A content in sputum ,which provides a brand-new way and method of treatment in dysfunctional ventilatory weaning response and lung injury related to ventilator .

OBJECTIVE: To research the prevalences of four kinds of bacteria including Alloiococcus otitidis, Streptococcus pneumonia, Haemophilus influenzae, and Moraxella catarrhalis in children with chronic otitis media with effusion (SOM) of the middle ear effusion, and the reproduction of the nasopharynx, so as to explore their meaning for the children with SOM. METHOD: Alloiococcus otitidis, Streptococcus pneumonia, Haemophilus influenza, and Moraxella catarrhal were investigated in the samples obtained from middle ear effusion and nasopharyn- geal swabs, using PCR and conventional bacterial culture methods. RESULT: By bacterial culture, the pathogen detection rate from middle ear effusion was 3.6%,while the nasopharynx was 54.0%, the detection rate of Streptococcus pneumonia, Haemophilus influenza, Moraxella catarrhalis was 10.8%, 27.0%, 4.5%, respectively, the drug susceptibility results for 51 samples of bacterial culture positive showed that 39 cases was sensitivite to the β-lactam antibiotic; By PCR, the number of detecting various kinds of bacteria simultaneously in middle ear effusion or in the nasopharynx were 6 and 34. The bacteria prevalences of S. pneumoniae, H. influenzae, M. catarrhalis, and A. otitidis are 5.4%, 5.4%, 3.6%, and 42.3% in the middle ear effusion, are 25.2%, 27.0%,13.5% and 34.2% in nasopharyngeal, respectively. CONCLUSION (1) PCR method is more sensitively detecting the bacteria than conventional bacterial culture methods. (2) The chronic SOM of children may be a combination of mixed bacterial infection, A. otitidis may be the most common pathogen of children SOM. (3) For children of SOM, if antibiotics are chosen to be used early in the disease, we suggest using the β-lactam antibiotics.
Bacteria ; Bacterial Infections ; complications ; Child ; Haemophilus influenzae ; isolation & purification ; Humans ; Moraxella (Branhamella) catarrhalis ; isolation & purification ; Nasopharynx ; Otitis Media with Effusion ; complications ; microbiology ; Polymerase Chain Reaction ; Prevalence ; Streptococcus pneumoniae ; isolation & purification