Teacher Lian Jianwei is good at treating internal diseases,however he is experienced at nose diseases too by differentiating signs and looking into inside from outside;now 3 medical concerned cases are extracted for reference.

AIM:To establish the method for quality control of Huisheng Diyidan Tablet(Eupolyphagea seu Steleophaga;Radix Angelicae sinensis,Sanguis Draconis,etc.). METHODS: Radix Angelicae sinesis,Eupolyphaga seu Steleophaga,Chinnabaris,Moschus,Boswellia carterii were identified by microscope.Sanguis Draconis was identified by TLC,Pyritum was identified by general identified reaction;The content limitation of cinnabaris(HgS) was determined by titration.Ferulic acid content of Radix Angelicae sinensis was determined by HPLC. RESULTS: Every part of the tablet was identified,the content limitation of cinnabaris(HgS) and ferulic acid content of Radix Angelicae sinensis were determined. CONCLUSION: The method showed that the characteristic for identification is distinct and highly specific,quantitative assay is simple,accurate,reliable and can be used for quality control of Huisheng Diyidan Tablet effectively.

Cleft Lip ; therapy ; Cleft Palate ; therapy ; Humans

Acute kidney injury(AKI) is a common consequence of perinatal asphyxia,occurring in up to 56％ of these infants,which can cause multiply organ dysfunction and neonatal death.This review summaries the development of early detection indexes in laboratory and clinic such as novel biomarker,urinary protein,urinary enzyme,renal blood flow velocity by pulse Doppler ultrasonography,and treatment in AKI post neonatal asphyxia.It provides more positive meanings on early detection,early management and eventually mortality reduction.

[Objective] To conclude the clinical experience of vertigo treatment using“wind tranquilizing method”by Pro. Lian Jianwei. [Method] Through analyzing clinical cases, generalizing the major syndrome, basic formula and formula modification of “wind tranquilizing method”used by Pro. Lian Jianwei to treat vertigo. [Result] The major pathogenesis of vertigo is wind, fire, phlegm, blood stasis and deficiency of blood. Pathological change of vertigo is in liver and so the treatment requires the tranquilizing of the liver-wind. Pro. Lian Jianwei is good at using“wind tranquilizing method”to cure vertigo caused by liver-wind stirring internal y. The formula of“wind tranquilizing method”takes into account the nature of vertigo and the physiological characteristics of the liver. He is also good at modifying the formula according to the patient conditions. [Conclusion] Pro. Lian Jianwei is good at prescrib-ing formula according to principles and treating methods. He also has deep understanding for the characteristic of vertigo and liver. Therefore the formula of his“wind tranquilizing method”has clinical value in terms of vertigo treatment.

Objective: To observe the influencing factors for efficiency of liposome-mediated transfection into human umbilical vein endothelial cell(HUVEC). Methods: Transferring HUVECs by distinct conditions,such as ratios of liposome and plasmid,densities of per well and different times of incubation,and transfection rates were observed and calculated by fluorescent microscope and flow cytometry. Results:⑴ When density of per well exceeded 2?l04 and times exceeded 4 hours,liposome-mediated transfection efficiency of endothelial cells decreased. ⑵With adding plasmid quality, transfection rates increased;while plasmid quality exceeded 1.0 ?g,in condition of 2?l04 per well and liposome volume 2 ?l, transfection efficiency reached the peak level. ⑶With adding liposome volume, transfection rates increased;while liposome volume was 8 ?l,in condition of 2?104 per well and plasmid quality 1 ?g, transfection efficiency was depressed.When ratios of liposome and plasmid were 1:6～1:8,the optimal transfection efficiency was 18.62%. Conclusion: Using optimal transfection parameter,we obtained the optimal transfection efficiency .

Objective:to investigate the status ofpatients with genitourinarytract infection caused byUreaplasma urealyticum(Uu)and Mycoplasma hominis(Mh),and its drug susceptibility and resistance in Chongqing.Methods:The genitourinary tract samples from 8 494 doubtful patients with mycoplasma infection were cultured and the susceptibility of the disease to 10 routine antibiotics was measured by mycoplasma cultivation,identification and drug susceptibility integration kit.Results:In the results of mycoplasma cultivation of 8 494 doubtful cases,2 735 cases were positive(32.2%),and 2 239 of them were simple Uu infection(26.4%),and 29 cases were simple Mh infection(0.3%)and 467 cases were double infection(5.5%).The results ofsusceptibilitytest showed that the sensitivityofUu toMinocycline,Deoxycycline,Clarithromycin,Azithromycin,JosamycinandRoxithromycinwas96.4%,95.4%,94.4%,87.1%,74.2%,and 73.3%,respectively,but tospectinomycin was 6.5%.Otherwise,the sensitivityofMh toDeoxycycline,Minocycline,Josamycin and Lincomycin was 100%,96.6%,96.6%,86.2% respectively,but toRoxithromycin,Azithromycin,Clarithromycin was 0%,0%,3.4% respectively.The double infection was the most sensitive to Minocycline and Deoxycycline,then to Josamycin,and the sensitivity was 92.1%,89.7% and 61.2%,respectively,but the sensitivityofittoRoxithromycin,Azithromycin,Clarithromycin,andSpectinomycinwas3.9%,5.2%,8.6%,and9.4%respectively.Conclusion:Mycoplasma is one of the main pathogen in genitourinary tract non-gonococcus inflammation,especially Uu infection.The therapy of mycoplasma infection should depress the production ofdrugresistant strain bythe reasonable adoption ofantibiotics accordingtothe results of susceptibilitytest.

Objective:To study the expession of TGF-? 2,TNF-? and its relationship with hepatitis B(HBV) infection in chronic hepatitis B,liver cirrhosis,paratumor cirrhosis and hepatocellular carcinomas(HCC).Methods:HBsAg,HBcAg,TGF-? 2 and TNF-? were detected by immunohistochemical staining among total 63 cases of liver cirrhosis,HCC,paratumor cirrhosis and normal liver tissues.Results:TGF-? 2 and TNF-? were expressed in liver cirrhosis,HCC and paratumor cirrhosis,and they showed significant difference from normal control liver( P c

Objective:To study the probable mechanism of HBV effect on cellular immune system in human body by detecting the quantity and function of CD4~+T and CD8+T cells in peripheral blood and liver tisues.Methods:1)The quantity of CD4~+T and CD8~+T cells in peripheral blood was detected by flow cytometry(FCM) in 36 patients with hepatitis B and 20 cases of controls;2)The expressions of HBsAg and HBcAg were detected by streptavidin-peroxidase immunohistochemistry(SP).50 cases of positive HBsAg and(or)HBcAg were selected as HBV(+) group,and 10 cases of negative HBsAg and HBcAg in liver tissue and blood serum were selected as HBV(-) group.The expressions of CD4,CD8,CD25 in liver tissues were detected in the two groups.Results:1)The quantity of CD4~+T cells and the ratio of CD4~+T/CD8~+T in peripheral blood of patients was significantly lower than those in controls;the quantity of CD8~+T cells was considerably higher than that in controls.(2)T lymphoid cell infiltrates in the liver were most gathered in the lobules and the portal tract.The densities of CD8~+T cells were higher in lobules and portal tracts than CD4~+T cells.The quantity of CD8+T and CD4~+T cells in the liver tissues of HBV positive group showed significant difference from the liver tissues of HBV negative group(P~b

In recent years,there are more and more medical disputes.Moreover, they are not easy to deal with.It has already become a focus problem to the society and a knotty problem to the hospitals.The author considers these facts that the patients don't know the facts of a case before approving doctor's treatment ad the hospitals absence of efficient proof are basic reasons.If we can do well the two jobs,they would play a positive role in preventing medical disputes and dealing with these accidents in high efficient,objective and impartial form.