Objective To investigate the different ability to produce H_(2)O_(2+) of the vaginal lactobacilli and their potential antagonism toward E.coli in ivtro.Then to select the potential lactobacilli strain to treat the female urogenital infection.Method To detect production of H_(2)O_(2) by measuring the blue matter in water solution with absorbtion spectrophotometry.The lactobacillus and E.coli were cultured together in solid culture medium,followed by the measurement of inhibition zone around the lactobacilli after 24 hour.Results We selected 4 strains of lactobacilli from vagina.L.acidophilus1 and L.paracasei3 are the stronger to produce H_(2)O_(2).The largest diameter of the ring of inhibition is shown around the lactobacilli acidophilus 1.Conclusion The lactobacilli acidophilus 1 and lactobacilli crispatus are the most potential strains as antagonist to E.coli and their antagonism depend not only on lacid or H_(2)O_(2).

Polo-like kinase 1 (PLK1) is a highly conserved serine/threonine protein kinase that has attracted research attention be-cause it plays a critical role in mitosis regulation. PLK1 is overexpressed in 80%of human tumors, which indicates a poor prognosis in most tumors. PLK1 is one of the most promising targets for antitumor therapy because it is upregulated in castrate-resistant prostate can-cer (CRPC). This review focused on the basic structure and function of PLK1, the relationship between PLK1 and CRPC occurrence and progression, and CRPC treatment by inhibiting PLK1. This study provides a theoretical basis for the targeted molecular therapy of CRPC.

To investigate the psychological difference in abdominal aortic aneurysm patients before and after operation and its influence on life quality, 24 patients who can understand and complete the questionnaire were enrolled in the study, the inquiry was performed after admission and discharge with the standard SCL 90 table. The results showed that the general conditions, depression, anxiety, compulsive symptoms and fear of the patients had marked differences before and after operation. It is concluded that operation of abdominal aortic aneurysm can not only prevent the rupture of aneurysm but also relieve the patients′ psychological pressuer and improve their life quality.

[Summary] Epithelial ovarian carcinoma has the highest fatality rate among gynecologic malignant tumors.Lacking of effective early diagnosis and screening method are the main reasons for its poor prognosis.There is a close relationship between the heterogeneity of epithelial ovarian carcinoma and the diversity of cancerous origins.According to the traditional theory, epithelial ovarian carcinoma derives from germinal epithelium at ovarian surface.The “secondary Mullerian system” theory has challenged above view.This“dualism” theory completely subverts the traditional one.However, both of the theories have their limitations.In recent years, the multiple origins theory of epithelial ovarian carcinoma has gradually become a hot point of discussion.

Objective To investigate influences of paeonol on mRNA expression and function of drug transporters BCRP and SLCO1B1 in HepG2 cell.Methods Cell counting Kit-8 assay was used to detect the viability of HepG2 cells;Real-time fluorescent quantitative PCR (qPCR) was performed to measure the expressions of BCRP and SLCO1B1 mRNAs; flow cytometry was applied to determine the transport functions of BCRP and SLCO1B1. Results Paeonol (2-8μg/mL) did not decrease HepG2 cell survival rate, but 16 μg/mL paeonol significantly reduced cell survival rate (P<0.05). Paeonol(2-8μg/mL)significantly induced the mRNA expression and function of drug transporters BCRP and SLCO1B1(P <0.05).Compared with control group, transcription level of paeonol group’s BCRP and SLCO1B1 drug transporters obviously up-regulated, the of translocation efficiency of substrate specificity increased significantly (P <0.05).Conclusion Paeonol can induce drug hepatocellular transporters BCRP and SLCO1B1 gene expression, thereby promote the substrate transport the transmembrane.It is indicated that the drug combination of paeonol and BCRP and SLCO1B1 transporters, there may be a risk of drug interactions.

ObjectiveTo investigate the causes, operation time, and microscopic repairing procedures of contusive iridodialysis. Methods23cases(23 eyes) who had partial iridodialysis due to contusions of the eyeballs underwent the microscopic repair during 7 to 18 days after injury. Viscoelastic agent and other auxiliary devices were used in the operation. ResultsAll eyes were fully corrected with approximate round-shaped pupils and their visual function improved. 1 eye with raised intraocular pressure was performed with trabeculectomy. ConclusionTimely repair of partial iridodialysis performed under microscope was safe and effective.

Objective To summarize the basic process to create epidemiological surveillance system. Methods First, make use of the system science, information science, control science and related disciplines as theory, use deductive reasoning to get the basic process of creating epidemiological surveillance system. Then, choose 50 existing epidemiological surveillance system to study, choose the characteristics of epidemiological surveillance system as the fundaction, use inductive reasoning to complete the basic process. Results The basic process of creating the epidemiological surveillance system include: system analysis, set up the logic model of the system, system design, system implementation, system evaluation, system improving. Conclusions Epidemiological surveillance system are indispensable aspects, they should be well integrated in order to ensure the effectiveness of created epidemiological surveillance system.

Setting criterion of teaching system,improving examination methods and strengthening the cultivation of basic medical skills and practice ability might be beneficial to the increase of the practice quality.

Objective To explore the impact of social support and perceived stigma on depression for people living with HIV/AIDS. Methods A total of 114 patients recruited into our study. Date were collected by Self-rating Depression Scale (SDS), Social Supporting Rating Scale (SRSS) and Berger HIV Stigma Scale (BHSS) for patients. Analysis of Moment Structures (AMOS) version 7.0 software was used to conduct the path analysis. Results The total score of patients′depression, SRSS and BHSS were (52.22± 10.28), (29.09±5.98), (109.02±14.59) points, and there was correlation between the three (r=0.425-0.676, P<0.05). Social support revealed direct effects on depression and affected depression indirectly via BHSS.The paths′ total coefficients was-0.47. BHSS as intermediary variable between social support and depression, the paths′ total coefficients was 0.64. Conclusions The level of depression, SRSS and BHSS were not fine for HIV/AIDS. Depression are directly affected by SRSS and BHSS, BHSS has a greater moderating effect on these relationships. Interventions developed on the basis of influencing factors of depression should be applied to improve patients′depression status.

Nucleos(t)ide analogues (NAs) have been widely used in clinical practice as first-line antiviral drugs.In recent years, many clinical studies have found that some patients cannot reach a satisfactory therapeutic outcome.This article introduces the antiviral indications and follow-up of chronic hepatitis B and summarizes the influence of baseline characteristics, HBV genotype, HBV mutation and drug resistance, patients'' medication compliance, presence or absence of other diseases or viral infection, and serum vitamin D level on the antiviral effect of NAs.It is pointed out that appropriate timing for antiviral therapy, patient education before treatment, regular follow-up during treatment, and timely identification of comorbidities and drug resistance help to improve the antiviral effect of NAs and delay the progression of liver diseases.