Objective To investigate the infection characteristics of human cytomegalovirus(HCMV)in infants of Huai′an area . Methods Clinical data of 72 infants with HCMV infection ,diagnosed from Nov .2013 to Feb .2015 ,were retrospectively analyzed , including HCMV DNA level of urine samples ,detected by using fluorescent quantitative‐polymerase chain reaction (FQ‐PCR) ,and complications and clinical characteristics .Results The positive rate of HCMV DNA was 90 .3% (65/72) .Among all of the infected infants ,60 cases (83 .3% ) were with pneumonia ,34 cases(47 .2% ) were with hepatitis ,14 cases(19 .4% ) were with hematological diseases ,3 cases(4 .2% ) were with neurological diseases ,and 28 cases(38 .9% ) were with two or more than two types of complica‐tions .Conclusion FQ‐PCR might be useful for the detection of HCM V infection .Clinical manifestation of pneumonia ,hepatitis ,he‐matological diseases ,and so on might be common in HCMV infected infants .FQ‐PCR ,used to detect HCMV DNA ,could be helpful for the early diagnosis and treatment of HCM V infection .

Hypoglycemia is a common and early metabolic disease of neonate.Serious and persistent hypoglycemia could result in brain damage.So far the pathogenesis of the disease is on research,but the definition of hypoglycemia,the glucose threshold of intervention,and diagnostic criteria of brain damage are still unclear.The pathogenesis of hypoglycemia is complicated.Brain damage may be related to the specific excitory amino acids receptors,reduced blood flow in occipital lobe,and cerebral hypoxia and ischemia.Diffusion weighted imaging of brain MRI and magnetic resonance spectroscopy are strongly recommended for the diagnosis of brain damage.Besides,clinical manifestation and cerebral function monitor are also included in the diagnosis.The threshold of intervention is suggested as serum glucose concentration with 2.2 ～ 2.6 mmol/L.

Actins ; metabolism ; Adenoma ; metabolism ; pathology ; Aged, 80 and over ; Desmin ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Leiomyoma ; metabolism ; pathology ; Smooth Muscle Tumor ; metabolism ; pathology ; surgery ; Thyroglobulin ; metabolism ; Thyroid Neoplasms ; metabolism ; pathology ; surgery ; Thyroidectomy ; Vimentin ; metabolism

Objective To investigate the change of lung morphology and the mechanism in the brain-dead state. Methods Ten Ba-Ma mini pigs were randomized into 2 groups: brain-dead group (group B, n=5) and control group (group C, n=5). Brain-dead model was established in group B by increasing intracranial pressure in a modified, slow and intermittent way, and brain-dead state maintained for 24 h by respiration and circulation support. The serum TNF-?, IL-1?, and IL-6 were determined at 3, 6 12, 18 and 24 h after the initial confirmation of brain death. Lung tissues were taken at 24 h. The alterations of lung tissues were observed by HE staining, and the expression of PKC-? detected by immunohistochemistry. PKC-? mRNA at each time point was detected by RT-PCR. The microstructure of hepatic tissues was observed under an electron microscope. Results (1) Under the light microscopy, broadened lung alveolar septum, edematous, extravasate in the alveolar congestive capillary vessel, infiltration of lymphocyte were observed. Under the election microscopy, cytoplasm edema, swollen mitochondria of the type-Ⅱ epithelial cells, partial membrane dissolution of mitochondria were observed and the microtomentum disappeared. No obvious morphological injury was observed under light microscopy and election microscopy. (2) Compared with control group, the serum IL-1?, IL-6 and TNF-? in brain-dead group began to increase at 3 h after brain death, and become more and more higher when the maintained state was prolonged. The expression of PKC-? and PKC-? mRNA in brain-dead group was increased at 24 h in the lung tissue. Conclusion Brain death may evoke lung morphological injury and increase the level of inflammatory mediators; After brain death the levels of PKC-? mRNA transcription and protein translation is increased. The activation of PKC-? and the alteration of inflammatory mediators are supposed to be one of the mechanisms of the lung injury.

[Objective]To investigate the effects of alendronate(ALN)on subchondral bone and articular cartilage in the rat osteoarthritis model induced by anterior cruciate ligament transaction(ACLT).[Method]Twenty-four three-month-old female Sprague-Dawley rats were divided randomly into three groups:Sham group(n=8),ACLT+NS group(n=8) and ACLT+ALN group(n=8)(20 ?g?kg-1?2 d-1 subcutaneous injection for 12 weeks).Bone mineral density(BMD) measurement and bone histomorphometric analysis were made for the subchondral bone of right distal femur and proximal tibia in all rats at 12 weeks post surgery.Degree of cartilage degeneration was scored by Mankin scoring system.Immunostaining for matrix metalloproteinase 13(MMP-13) was performed to investigate the effect of ALN on cartilage matrix loss.[Result]BMD of the subchondral bone in the ACLT+NS group were significantly lower than those in the Sham group and the ACLT+ALN group(P

Purpose:To analyze the differences between prem enopausal and postmenopausal female patients with T1 primary breast cancer as to pathological classification, rate of lymphatic metastasis and some relative rec eptors, and to discuss the appropriate mode of operation to T1 breast cancer. Methods:154 patients with T1 primary breast cancer were retrosp ectively divided into premenopausal group and postmenopausal group. The clinical data of the two groups were compared. Results:There were no significant statistical differences in pr imary tumor size, but the incidence rate(62.2%) of invasive ductal cancer in po stmenopausal group was less than that(84.7%) of postmenopausal group(P

[Objective]To assess the protective effects of Xianlinggubao on articular cartilage and subchondral bone in experimental osteoarthritis induced by anterior cruciate ligament transaction (ACLT)in rabbits. [Methods]Thirty healthy Japanese white rabbits were divided randomly into three groups:sham group,ACLT+XLGB group and ACLT+ NS group. Rabbits in ACLT+XLGB group and ACLT+NS group received anterior cruciate ligament transection(ACLT)on the right knee. Rabbits in sham group received untreatment as control. ACLT+XLGB group received a daily administration of XLGB at a dose of 125 mg/kg of body weight for 6 weeks.In contrast,ACLT+NS group received saline at the same dose. All rabbits were sacrificed at 6 weeks after operation. The right femora were removed,measured for bone mineral density (BMD),and then were observed for the macro-pathologic changes with HE staining and graded by Mankin's scale. Expression levels of BMP-2 and MMP-13 were detected by immunostaining. The subchondral bone of right tibial plateau was cut and stained for the measurement of bone histomorphometry.[Results](1)The Mankin's score in ACLT+ XLGB group was significantly lower than that in ACLT+ NS group (P

Objective To investigate Hysterothylacium aduncum (Anisakidae) infection in marine fishes from Bohai Sea and Yellow Sea. Methods Nematodes were collected from the digestive tract of fishes, fixed with hot 4% formalin and preserved in 70% ethanol for study. The specimens were cleared in lactophenol for light microscopical examination, and properly treated for scanning electron microscopy (SEM). Results Among the fishes examined, 14 out of 93 species (15.1%) were found infected by H.aduncum, with a higher prevalence in the fish of Lophius litulon(66.7%), Scomber-omorus niphonius(47.5%), and Gadus macrocephalus(33.3%). H.aduncum infection was first recorded in elasmobranch-Raja smirnovi. Morphological differences of H.aduncum were observed, including the width of lateral alae and the length of intestinal caecum. Conclusion H.aduncum in fishes of Bohai Sea and Yellow Sea in China may be a complex species, and its high prevalence in some fishes reminds the risk of anisakiasis by eating raw fishes.

Objective To study the clinical features and significance of the patients with dermatomyositis(DM)associated with interstitial lung disease(ILD).Methods216cases of DM were collected from the Peking Union Medical College Hospital from1970to1997.The criteria for the diagnosis of DM were based on the report of Bohan and Peter in1975.The mean follow-up period of these patients was5.8?4.7years(ranged from2to27years).The clinical manifestations and laboratory findings of32DM patients with ILD were analyzed and compared with those of184DM patients without ILD.Results As compared with DM patients without ILD,the DM patients with ILD were more likely to have fever(28.2%vs.6.5%,P

Objective To explore the kinds of Traditional Chinese Medicine(TCM)syndromes of depression to establish the criteria of common TCM syndromes of depression. Methods According to the ways of clinical epidemiology, TCM Bianzheng and syndromes’ indexes were investigated in 1977 depression patients with unipolar and bipolar affective disorder in 8 investigation sites such as Hunan, Tianjin, Beijing, Shenzhen, Guanxi, Hujian and Guizhuo. Results There were 12 kinds of syndromes in depression. According to order of composition ratio, the first four syndroms respectively were Gan-Yu-Qi-Zhi, Gan-Yu-Pi-Xi, Gan-Yu-Tan-Zu, Xin-Pi-Liang-Xu, the ratios of which were 29 7%, 24 5%, 13 4% and 12 8% respectively.Conclusions The common TCM syndromes of depression are such as the followings: Gan-Yu-Qi-Zhi, Gan-Yu-Pi-Xi, Gan-Yu-Tan-Zu, Xin-Pi-Liang-Xu. There are some relationships between common TCM syndromes of depression and 4 sub-types of unipolar disorder.