Objective To investigate the infection characteristics of human cytomegalovirus(HCMV)in infants of Huai′an area . Methods Clinical data of 72 infants with HCMV infection ,diagnosed from Nov .2013 to Feb .2015 ,were retrospectively analyzed , including HCMV DNA level of urine samples ,detected by using fluorescent quantitative‐polymerase chain reaction (FQ‐PCR) ,and complications and clinical characteristics .Results The positive rate of HCMV DNA was 90 .3% (65/72) .Among all of the infected infants ,60 cases (83 .3% ) were with pneumonia ,34 cases(47 .2% ) were with hepatitis ,14 cases(19 .4% ) were with hematological diseases ,3 cases(4 .2% ) were with neurological diseases ,and 28 cases(38 .9% ) were with two or more than two types of complica‐tions .Conclusion FQ‐PCR might be useful for the detection of HCM V infection .Clinical manifestation of pneumonia ,hepatitis ,he‐matological diseases ,and so on might be common in HCMV infected infants .FQ‐PCR ,used to detect HCMV DNA ,could be helpful for the early diagnosis and treatment of HCM V infection .

Hypoglycemia is a common and early metabolic disease of neonate.Serious and persistent hypoglycemia could result in brain damage.So far the pathogenesis of the disease is on research,but the definition of hypoglycemia,the glucose threshold of intervention,and diagnostic criteria of brain damage are still unclear.The pathogenesis of hypoglycemia is complicated.Brain damage may be related to the specific excitory amino acids receptors,reduced blood flow in occipital lobe,and cerebral hypoxia and ischemia.Diffusion weighted imaging of brain MRI and magnetic resonance spectroscopy are strongly recommended for the diagnosis of brain damage.Besides,clinical manifestation and cerebral function monitor are also included in the diagnosis.The threshold of intervention is suggested as serum glucose concentration with 2.2 ～ 2.6 mmol/L.

Actins ; metabolism ; Adenoma ; metabolism ; pathology ; Aged, 80 and over ; Desmin ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Leiomyoma ; metabolism ; pathology ; Smooth Muscle Tumor ; metabolism ; pathology ; surgery ; Thyroglobulin ; metabolism ; Thyroid Neoplasms ; metabolism ; pathology ; surgery ; Thyroidectomy ; Vimentin ; metabolism

Objective To investigate the change of lung morphology and the mechanism in the brain-dead state. Methods Ten Ba-Ma mini pigs were randomized into 2 groups: brain-dead group (group B, n=5) and control group (group C, n=5). Brain-dead model was established in group B by increasing intracranial pressure in a modified, slow and intermittent way, and brain-dead state maintained for 24 h by respiration and circulation support. The serum TNF-?, IL-1?, and IL-6 were determined at 3, 6 12, 18 and 24 h after the initial confirmation of brain death. Lung tissues were taken at 24 h. The alterations of lung tissues were observed by HE staining, and the expression of PKC-? detected by immunohistochemistry. PKC-? mRNA at each time point was detected by RT-PCR. The microstructure of hepatic tissues was observed under an electron microscope. Results (1) Under the light microscopy, broadened lung alveolar septum, edematous, extravasate in the alveolar congestive capillary vessel, infiltration of lymphocyte were observed. Under the election microscopy, cytoplasm edema, swollen mitochondria of the type-Ⅱ epithelial cells, partial membrane dissolution of mitochondria were observed and the microtomentum disappeared. No obvious morphological injury was observed under light microscopy and election microscopy. (2) Compared with control group, the serum IL-1?, IL-6 and TNF-? in brain-dead group began to increase at 3 h after brain death, and become more and more higher when the maintained state was prolonged. The expression of PKC-? and PKC-? mRNA in brain-dead group was increased at 24 h in the lung tissue. Conclusion Brain death may evoke lung morphological injury and increase the level of inflammatory mediators; After brain death the levels of PKC-? mRNA transcription and protein translation is increased. The activation of PKC-? and the alteration of inflammatory mediators are supposed to be one of the mechanisms of the lung injury.

[Objective]To investigate the effects of alendronate(ALN)on subchondral bone and articular cartilage in the rat osteoarthritis model induced by anterior cruciate ligament transaction(ACLT).[Method]Twenty-four three-month-old female Sprague-Dawley rats were divided randomly into three groups:Sham group(n=8),ACLT+NS group(n=8) and ACLT+ALN group(n=8)(20 ?g?kg-1?2 d-1 subcutaneous injection for 12 weeks).Bone mineral density(BMD) measurement and bone histomorphometric analysis were made for the subchondral bone of right distal femur and proximal tibia in all rats at 12 weeks post surgery.Degree of cartilage degeneration was scored by Mankin scoring system.Immunostaining for matrix metalloproteinase 13(MMP-13) was performed to investigate the effect of ALN on cartilage matrix loss.[Result]BMD of the subchondral bone in the ACLT+NS group were significantly lower than those in the Sham group and the ACLT+ALN group(P

Objective To investigate Hysterothylacium aduncum (Anisakidae) infection in marine fishes from Bohai Sea and Yellow Sea. Methods Nematodes were collected from the digestive tract of fishes, fixed with hot 4% formalin and preserved in 70% ethanol for study. The specimens were cleared in lactophenol for light microscopical examination, and properly treated for scanning electron microscopy (SEM). Results Among the fishes examined, 14 out of 93 species (15.1%) were found infected by H.aduncum, with a higher prevalence in the fish of Lophius litulon(66.7%), Scomber-omorus niphonius(47.5%), and Gadus macrocephalus(33.3%). H.aduncum infection was first recorded in elasmobranch-Raja smirnovi. Morphological differences of H.aduncum were observed, including the width of lateral alae and the length of intestinal caecum. Conclusion H.aduncum in fishes of Bohai Sea and Yellow Sea in China may be a complex species, and its high prevalence in some fishes reminds the risk of anisakiasis by eating raw fishes.

Objective:To evaluate the effects of different enamel conditioners in combination with an adhesive on the shear bond strength and the adhesive remnant index in vitro.Methods:One hundred and eight permanent healthy premolars from orthodontic patients and fresh saliva from a healthy young volunteer were collected.The teeth were cleaned,dried and etched by phosphoric acid,polyacrylic acid or Transbond plus SEP respectively(36 teeth for each agent).Then the teeth were bonded to brachet by RMGICs with the presence of water,saliva on the tooth surface or without any of them respectively(12 teeth in each group).After bonding,all samples were tested on a testing machine of Instron.Shear bond strength and adhesive remnant index were recorded.Results:Highest mean shear bond strength was observed in the group of teeth etched by Traspond plus SEP and bonded by RMGICs with the presence of saliva.Higher mean shear bond strength was obtained with the presence of saliva or water on the tooth surface than without any of them.Conclusion:Transbond plus SEP conditioning followed by RMGICs bonding may produce high shear bond strength between moistured tooth surface and brachet.

Objective: To express glycosyl-phosphatidylinositol (GPI) modified Met- RANTES fusion protein on Chinese hamster ovary (CHO) cells and to develop a novel immunosuppressant GPI anchored form of Met-RANTES. Methods: The eukaryotic expression vector PEF/GPI-Met-RANTES were constructed and transfected into CHO cells by electroporation. The transfectants were selected with methotrexate (MTX). Expression of the recombinant protein was assessed by flow cytometric analysis, cell immunofluorescence staining and immunogold electron microscopy. Results: The chimeric molecules of GPI anchored form of Met-RANTES including the whole reading frame were constructed, and the sequence was identical to the designed sequence. GPI anchored form of Met-RANTES was stably expressed on CHO- DHFR- cells. Conclusion: A large amount of GPI modified Met-RANTES fusion protein was expressed on CHO cells. GPI anchored form of Met-RANTES may be used as novel immunosuppressant for suppressing reaction in graft rejection.

There is immense interest in the long-chain polyunsaturated fatty acids(LC-PUFAs) on account of their health-beneficial properties.There has been considerable interest in the production of LC-PUFAs in transgenic plants to provide a cheap,sustainable and clean source of these fatty acids.Several main LC-PUFAs,their roles and metabolic pathway in plant are introducedbriefly,progresses on biosynthesis of LC-PUFAs in transgenic plant are reviewed,and strategies for improving the yields of LC-PUFAs in plant are also discussed.

Many unusual fatty acids present in the seed oils of non-food plants are modified at the ?12 position either by the addition of epoxy or hydroxy groups or by the formation of triple (acetylenic) bonds or conjugated double bonds. The introduction of these functionalities into C18 fatty acids is catalyzed by a family of divergent forms of the fatty-acid ?12-desaturase (FAD2) enzyme. Genes encoding these enzymes have now been cloned from a variety of organisms and expressed in transgenic oilseeds. However,the accumulation of the intended fatty acids in transgenic plants has been relatively disappointing,and the mechanisms of these unusual fatty acids biosynthesis and transferring from phosphatidylcholine into storage triacylglycerols still need to be elucidated.