Hemangioma ; metabolism ; pathology ; Humans ; Immunohistochemistry

Adult ; Aged ; Antineoplastic Combined Chemotherapy Protocols ; administration & dosage ; therapeutic use ; Boronic Acids ; administration & dosage ; therapeutic use ; Bortezomib ; Female ; Humans ; Male ; Middle Aged ; Multiple Myeloma ; drug therapy ; Pyrazines ; administration & dosage ; therapeutic use

Objective To explore the attachment of children with emotional disorder,and provide a theoretical basis for mental intervention.Methods A total of 70 outpatients with emotional disorder and their parents and 140 normal children and their parents were enrolled.All the children and their parents were assessed with Adolescent Attachment Inventory and Experiences in Close Relationships Inventory and General demographic information inventory.Results There were statistical significances in family proximity((12.76 ± 3.69) vs (15.47 ±3.05)),friend proximity ((14.23 ± 3.84) vs (15.82 ± 3.06)) and family negativity ((12.84 ± 3.42) vs (10.46 ± 2.94)) of two groups (all P ＜ 0.05).There were statistical significances in maternal attachment avoidance ((3.60 ± 0.70) vs (3.84 ± 0.63)) and anxiety ((3.23 ± 0.77) vs (3.37 ± 0.79)) of two groups (all P ＜0.05).In emotional disorder group,there was a significantly negative relation (P ＜ 0.01) between parental anxiety dimension and friend dependence factor.Conclusion The attachment of children with emotional disorser is low family and friends proximity,higher family negativity.

AlM: To analyze the correlation between tear film changes of allergic conjunctivitis and dry eye, then provide clinical references for better treatment.
METHODS: Fifty patients with allergic conjunctivitis were taken as the observation group, the control group was selected based on 1:1 case control theory, and we chose 50 health volunteers without ocular surface diseases, xerophthalmia and systematic diseases randomly, then fluorescein ( FL) staining, break-up time ( BUT) , Schirner l test ( SⅠt) , tear meniscus high ( TMH) and slit-lamp examinations were performed in the two groups.
RESULTS: ln the observation group, FL, BUT, Slt, TMH of right eyes and left eyes were statistically significant correlated ( P < 0. 05 ). FL and BUT were statistically significant between control group and observation group (P<0. 05). TMH and Slt has positive correlation, while FL and BUT has negative correlation and the differences were statistically significant (P<0. 05). CONCLUSlON: Due to inflammatory mediators participation, allergic conjunctivitis could lead to the stability changes of tear film which cause in dry eye. The stability changes of allergic conjunctivitis correlate to the damage degree of epithelium.

AIM: To investigate the situation of patients with mechanical ocular trauma and the possible influencing factors related to the prognosis.METHODS: Totally 260 patients(286 eyes) with mechanical ocular trauma from January 2011 to January 2016 in Xiaogan Central Hospital were selected and retrospective studied.Sociology and clinical data including gender,age,career,injury causes,injury nature,treatment time and visual acuity on admission in these patients were univariate analyzed,then the positive factors were analyzed using Logistic regression.RESULTS: The middle-aged and young males were domain in 260 patients.The causes were mainly sharp instrument injury,while perforating injury of eyeball was the majority in injury nature.Univariate analysis showed that injury causes,injury nature,treatment time and eyesight on admission were influencing factors,while multivariate analysis by Logistic regression showed that visual acuity on admission and orbital contusion were independent influencing factors for patients ocular trauma.CONCLUSION: Ocular trauma is a majoy cause of monocular blind,there are close associations between prognosis of ocular trauma and orbital contusion and visual acuity on admission.But the majority of ocular trauma is preventable and treatable,the relevant measures should be developed to prevent the ocular trauma.

Mutual information(MI) is a tool of measuring the statistical dependence between two random variables. MI between two images, being considered as two random fields, can be calculated to determine the degree of information redundancy between the image intensities of corresponding pixels in both images. In this paper, we utilize the MI criterion to realize the generalized registration between two neighboring slices in a medical image sequence (CT, MR et al) and consider that they match with each other if the MI between them is maximal. Experimental results confirm performance of our algorithm.

Objective To evaluate the correlation between fluid balance and myocardial function and the mortality in patients with septic shock.Methods Ninety two septic shock patients enrolled between January 2013 and January 2015 were divided into survival group and non-survival group based on 28-day mortality.Fluid balance,haemodynamic parameters and B-type natriuretic peptide (BNP) were collected on days1,3,7 after admission.Results Forty four patients survived the crisis while 48 died.The 7 day average fluid balance volume in survival group was lower than those in non-survival group [(55 ± 9)ml/kg vs.(187 ± 14)ml/kg,t =54.260,P ＜0.01].The initial left ventricle ejection fraction (LVEF) in survival group was lower than that in non-survival group [(42％ ±4％) vs.(54％ ±6％),t =10.100,P ＜0.01].Serum BNP level was significantly lower than those in non-survival group [(267 ± 54) ng/L vs.(681 ± 22) nig/L,t =48.580,P ＜0.01].Multivariate logistic regression analysis showed that fluid positive balance,LVEF and acute physiology and chronic health evaluation Ⅱ (APACHEⅡ) were the independent predictors for 28 day mortality (P ＜ 0.05).Conclusions Fluid balance and myocardial function can serve as risk predictors for septic shock patients.

BACKGROUND:Current research has shown that tumor necrosis factorαstimulated gene 6 (TSG-6) has anti-inflammatory effect, and the scar formation can be inhibited by local injection of TSG-6 protein at the early stage of trauma. However, the mechanism of this effect is stil unclear. OBJECTIVE:To construct the lentiviral expression vector and shRNA vector for human TSG-6, with stable overexpression, transfection and interference, and to explore the effect of TSG-6 on proliferation and apoptosis of keloid fibroblast cel lines. METHODS:Human keloid fibroblast cel s were isolated from the keloid’s tissue by enzyme digestion and identified by immunocytochemistry assay. Lentiviral vectors pLVX-puro-TSG-6 and pLVX-shRNA1-TSG-6 were constructed and transfected into human keloid fibroblast, exclusively. Expression levels of TSG-6 mRNA and protein were detected by RT-PCR and western blot assay. MTT assay and flow cytometry were used to estimate the cel proliferation and apoptosis in each group after transfection. In addition, expression of Bcl-2, p53 and active-caspase-3 were detected by western blot assay in each group. RESULTS AND CONCLUSION:(1) Human keloid fibroblasts were separated successful y. Under the light microscope, cel s were spindle. Immunohistochemical staining for vimentin was performed in the fifth passage of cel s, with the positive rate of 100%. Cel s were negative for cytokeratin. (2) Recombinant lentiviral vectors and stably transfected cel lines were successful y established. TSG-6 gene expression was altered apparently. Compared with the control group, cel proliferation was delayed and apoptotic rate was noticeably increased in TSG-6 gene overexpression group. Cel proliferation increased and apoptotic rate decreased in the TSG-6 gene intervention group (P<0.05). (3) Western blot assay results demonstrated that Bcl-2 expression reduced, P53 and Active-caspase-3 expression significantly increased in the TSG-6 gene overexpression group (P<0.05). (4) These finding showed that TSG-6 could inhibit proliferation and induce apoptosis in keloid fibroblasts. Its mechanism may be associated with the down-regulation of Bcl-2 protein expression, up-regulation of P53 protein expression and increased Caspase-3 activity.

The Medical College of Panzhihua University has developed a series of curriculum reform based on Medical Professionalism, investigating the basic idea, curriculum design and assess-ment system of Medical Professionalism curriculum reform, emphasizing the combination of medical professionalism curriculum with clinical courses, in the hope of providing a unified and acknowledged medical professionalism curriculum.