Humans ; Lichen Planus, Oral ; psychology

Objective To observe the inhibit effect of triplex forming oligonucleotide (TFO)(platelet-derived growth factor-B chain, PDGF) on tumor growth and angiogenesis in rats with glioma.Methods 1?10~6 C_6 glioma cells with high-flow microinfusion were seed into right caudate putamens of 18 rats by stereotaxic technique. TFO was injected in situ 1 week after glioma cells inoculation. Treat group Ⅰ and treat group Ⅱ received TFO at dose of 1.5 mg/20 ?l and 3.0 mg/20 ?l, respectively. The same doses were given again at 8, 11 and 14th day after glioma cells inoculation. The control group was treated with 20 ?l normal saline at same time like treat groups. Three weeks after glioma cells inoculation, all the rats were killed. The expressions of, PDGF-B, vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) were detected with microscopic histology.Results The inhibition rate of tumor growth was 66.0% in treat groupⅠand 92.2% in treat group Ⅱ. There was significant difference between the two groups ((P

Objective To express and purify the killer immunoglobulin-like receptor KIR3DL1 extracelluar domain.Methods pUC57-KIR3DL1 was used as template,KIR3DL1 extracelluar domain was amplified by polymerase chain reaction(PCR)and cloned into pGEM-T vector with A-T cloning technique.After DNA sequence analysis,the target fragment was inserted into the prokaryotic expression vector pET28a-DsbA to construct the recombinant vector pET28a-DsbA /KIR3DL1.The recombinant plasmid was transformed into E.coli BL21(DE3),and induced with IPTG.Bacterial pellets were resuspended in 8M urea and centrifuged to remove the insoluble material.The crude extract was purified by passing over a Ni-NTA-agarose column.After the inclusion body flowing through the Ni-NTA-agarose affinity chromatography was refolded successfully,it was purified by Superdex75 gel filtration and the purification effects of the fusion protein were identified by SDS-PAGE and western blot.Result Some fusion proteins were expressed in the supernatant,and the others were expressed in the form of inclusion bodies.The purity of fusion protein was over 95% after purification under denaturing condition.Conclusion The highly efficient expression of KIR3DL1 extracelluar domain laid the foundation for the further studies on exploration of the mechanism of immunization recognition between KIR3DL1 and its ligand.

Objective To investigate the incidence of renal injury caused by strong intensity military training in the navy.Methods One thousand three hundred and twenty servicemen of the navy were enrolled in the present study including 568 recruits and 752 veterans.Urine protein and hemoglobin were determined using the dry chemistry method after a five-kilometer armed field race.Urine samples were collected and centrifuged for erythrocyte count under microscope after training.Retinol binding protein(RBP) and N-acetyl-?-D-glucosaminidase(NAG) were measured by enzyme linked immunosorbent assay(ELISA).Detection of creatine kinase(CK) above 950U/L was assumed to be rhabdomyolysis positive.Results The average incidence of hematuria,hemoglobinuria and proteinuria was 2.1%,7.4% and 44 %,respectively,and the incidence of abnormal RBP and NAG was 57.3% and 57.1%,respectively.Significant difference in each of the indexes existed between the recruits and veterans(P

PURPOSEObservation of clinical efficacy of idarubicin in the treatment of patients with both newly-diagnosed and relapsed/refractory acute leukemia. METHODS 34 patients of acute leukemia enrolled in this study are classified into 3 groups: newly-diagnosed, relapsed and refractory group. All patients are treated with combination chemotherapy composed of idarubicin and other agents. RESULTS The response rate for all patients is 70. 6% while the result in newly-diagnosed group is superior to both relapsed and refractory group (P

This study was aimed to assess the proportion and causes of misdiagnosis of pancreatic cancer (PC), and the effects of misdiagnosis on and treatment of PC, so as to sum up experiences and improve the diagnosis and treatment. 1027 cases with confirmed diagnosis of PC were retrospectively analyzed in detail. The patients were divided into misdiagnosed group and a group with correct diagnosis. The relevant data of the two groups were analyzed by stastical methods, and the differences between them were assessed. It was found that 586 cases (57 1%) were misdiagnosed.Fifty five diseases were involved in misdiagnosis, among them gastritis and duodenitis, peptic ulcer, virus hepatitis, cholecystitis and cholelithiasis, chronic pancreatitis wese the commonest. The differences of duration of diagnosis, main symptoms, resection rate, and half year survival rate between misdiagnosed group and correct diagnosis group were significant. It is concluded that PC is often misdiagnosed as other diseases. Early diagnosis and treatment is critical to the prognosis. The physician should be aware that PC may present a veriety of clinical manifestations, and therefore he or she should analyze comprehensively the symptoms and signs in order to improve diagnosis and treatment.

To study the relationship between the occurrence of pancreatic cancer with diabetes mellitus, other medical conditions and familial history in Chinese population. A case control study comprising 493 histologically confirmed pancreatic cancer patients and 1031 hospital non neoplastic controls matched with age, sex and economical income was conducted. The value of odds ratio (OR) and its 95% confidence interval (CI) to estimate the relative risk of diabetes mellitus, other medical conditions and familial history were calculated. The results showed that ORs and 95%CIs of diabeties diagnosed 2 years prior to the diagnosis of pancreatic cancer, cholelithiasis, chronic pancreatitis, cancer history, pulmonary tuberculosis, Helicobacter pylori infection, family history of cancer and family history of diabetes mellitus were 4 64(2 05～10 49),4 12(2 81～6 04),18 38(6 33～53 35),9 47(4 97～18 06),4 21(2 30～7 72),1 14(0 45～2 89),2 01(1 29～3 14), 0 83(0 15～4 56) respectively. The p values of diabeties and cholelithiasis in logistic regression analysis were less than 0 05. The analysis suggested that diabetes mellitus, cholelithiasis, chronic pancreatitis, cancer history, pulmonary tuberculosis, and family history of cancer were risk factors of pancreatic cancer, whereas diabetes mellitus and cholelithiasis were the independent factors.

Smoking is presently the only generally accepted risk factor for pancreatic cancer. In recent studies, reproductive history has been associated with pancreatic cancer, but with contradictory results. In order to evaluate a possible association between age of first parturition and the number of births and pancreatic cancer, we conducted a case control study with a hospital background. Association between age of first parturition and the number of births and pancreatic cancer was found, and it remained after adjustment for cigarettes smoking, alcohol consumption, body mass index, cholelithiasis, cholecystectomy, diabetes mellitus, chronic pancreatitis and partial gastrectomy. Young age of first parturition and high reproductive history, such as 3 or more births were risk factors for pancreatic cancer.

The study was to analyze the correlation between pancreatic cancer and appendectomy, partial gastrectomy, cholecystectomy and tonsillectomy in Chinese population. Case control study was made comprising 493 pancreatic cancer patients confirmed by histology and 1031 hospitalized non neoplastic controls matched with age, sex, residing district, and economical income. The value of odds ratio (OR) and its 95% confidence interval (CI) were calaclated to estimate the relative risk of appendectomy, partial gastrectomy, cholecystectomy and tonsillectomy to the occurrence of pancreatic cancer, and logistic regression was conducted. The results showed that the risk of pancreatic cancer increased in patients who had had appendectomy, partial gastrectomy and cholecystectomy, and all the p values of trend test were

To study the clinical features of the pancreatic cancer (PC), clinical symptoms of 1027 diagnosed cases of PC were retrospectively analyzed in detail, including their dynamic changes with regards to the patients'sexes, ages, blood types, and tumor locations in the pancreas. The results showed that abdominal pain, jaundice and epigastric discomfort were the prevailing initial symptoms in these 1027 cases. Abdominal pain, jaundice, weight loss, and anorexia were the main symptoms. Unusual symptoms were seemed to be different in different patients. The clinical symptoms could be dramatically different with difference in patients′ age and tumor location. The blood type might also infhluence the clinical symptoms in certain degree. It is concluded that the clinical symptoms of the PC were atypical in general, but still there were some characteristics.The physician should grasp these characteristic symptoms in order to make an early diagnosis.