Atherosclerosis ; Dyslipidemias ; Humans

Objective To investigate the effects of different ventilations during the induction of anesthesia on postoperative nausea and vomiting in patients undergoning laparoscopic cholecystectomy. Methods One hundred and twenty patients undergoing laparoscopic cholecystectomy were randomly divided into M group and V group according to random number table, 60 patients each group. The patients in M group performed manual ventilation and the patients in V group performed mechanical ventilation. According to different tidal volume, V group were randomly divided into three groups (20 patients each group:V1,V2 and V3 group, respectively recieved body weight × 4, 6 and 8 ml/kg. After tracheal intubation,the operations were completed by maintaining the end of CO2 partial pressure (PETCO2)from 35 to 45 mmHg, 1 mmHg = 0.133 kPa. Tidal volume, peak airway pressure (Ppeak) and PETCO2 in anesthesia induction,and intraoperative gastric insufflation and postoperative nausea and vomiting were recorded and compared. Results The incidence rate of gastric insufflation, postoperative nausea and vomiting in V group were significantly higher than those in M group:50.0%(30/60) vs. 21.7%(13/60), 45.0%(27/60) vs. 18.3%(11/60), 26.7%(16/60) vs. 10.0%(6/60), P<0.05. The level of Ppeak in M group, V2 group and V3 group were signicantly higher than that in V1 group: (13.55 ± 1.95), (12.05 ± 1.23), (18.35 ± 3.71) cmH2O vs. (13.55 ± 1.95) cmH2O, 1 cmH2O = 0.098 kPa, P<0.05. The level of PETCO2 in M group, V2 group and V3 group were signicantly lower than that in V1 group:(19.60 ± 4.31), (27.75 ± 1.94), (23.60 ± 4.45) mmHg vs. (29.90 ± 2.40) mmHg, P<0.05. Conclusions Manual ventilation during the induction of anesthesia in patients undergoning laparoscopic cholecystectomy is better than mechanical ventilation . Proper manual positive pressure ventilation and volume mechanical ventilation with low tidal (4 ml/kg) may allow for decreasing intraoperative gastric insufflation and postoperative nausea and vomiting.

OBJECTIVE:To investigate the characteristics and regularity of ADR in our hospital,and to provide reference for clinical rational drug use. METHODS:262 ADR reports of the hospital during 2013-2014 were summarized and analyzed. RE-SULTS:Among 262 cases of ADR,most of ADR occurred in elderly patients aged above 60 years old,accounting for 46.56%. 239 cases were induced by intravenous drip,accounting for 91.22%;anti-infective drugs induced 97 cases of ADR,accounting for 37.02%,among them there were 48 cases of cephalosporin-induced ADR,accounting for 49.48%(total 97 cases);most of them were lesion of skin and its appendants,being 96 cases and accounting for 36.64%. Among 262 cases,132 cases were improved af-ter drug withdrawal(50.38%);no drug sequelae or death cases was found. CONCLUSIONS:To master the characteritics and reg-ularity of ADR contribnte to redcue and aroid the repeat of ADR.

Animals ; Autoantigens ; genetics ; metabolism ; physiology ; Centromere Protein A ; Chromosomal Instability ; Chromosomal Proteins, Non-Histone ; genetics ; metabolism ; physiology ; Gene Expression Regulation, Neoplastic ; Humans ; Kinetochores ; metabolism ; Mitosis ; physiology ; Rectal Neoplasms ; genetics ; metabolism ; pathology ; Spindle Apparatus ; metabolism

Maximum Allowable Concentration ; Mineral Fibers ; analysis ; Occupational Exposure ; analysis

Objective To investigate the histogenesis and clinicopathologic features of carcinosarcoma of the urinary bladder. Methods Two cases of carcinosarcoma (one man and one woman)were included.They were admitted to hospital with discontinuous,painless,macroscopic hematuria.Clinicopathalogic features of all findings of the 2 cases were reviewed. Results Cystoscopy of both cases showed that the tumors displayed polypous or cauliflower-like shape,and grew invassively.CT examination of the bladder wall confirmed the presence of parenchymatous tumor.Both of the 2 cases underwent partial cystectomy.Intraoperative examination showed the tumors were similar to carcinoma of bladder.Histopathology showed biphasic neoplasms with distinct high grade epithelial and mesenchymal components.Morphologic characteristic of the tumor was abnormally proliferative and the mitotic figures could be seen.One of the patients died within 11 months and the other,16 months following the operation. Conclusions Carcinosarcoma of the urinary bladder is a kind of rare malignant tumor that is most often in an advanced stage at presentation and has an unfavorable prognosis. It should be identified promptly and treated appropriately.Those who have discontinuous,painless,macroscopic hematuria should be warned of the risk of the disease.

Objective To study the gene mutation in a pedigree with Dowling-Meara type epidermolysis bullosa simplex (DM-EBS). Methods Using the immuno-histochemistory, electron microscopy, PCR-HA and DNA sequencing. Results The cytolysis was observed throughout the lower basal layer, tonofilaments were decreased; using PCR-HA, the K14 gene mutation was detected. By DNA sequencing, we found gene mutation in this pedigree: K14 1A domain: N123R. Conclusion There is K14 1A domain gene mutation in this DM-EBS pedigree.

Objective To study ischemic tolerance induced by acanthopannx senticosus saponins(ASS) on ischemia in PC12 cells and involve expression of hypoxia inducible factor-1?(HIF-1?).Methods An ischemic model was developed in PC12 cell line with treatment of oxygen glucose deprivation(OGD).The protective effects of ASS pretreatment on ischemic tolerance of PC12 cells and whether protective effects of ASS could be inhibited by LY294002(PI3K inhibitor) were analyzed through MTT assay.The induction of phospho-glycogen synthesis kinase-3?(p-GSK-3?) and HIF-1? after pretreatment of ASS and possible blocking effects of LY294002 were detected by Western-blot.Results MTT results showed that after 9 h ischemia,the viability of PC12 cells decreased dramatically(P

Objective To investigate the mechanisms of phenytoin accelerating healing process.Methods Adult male Wistar rats that survived ligatation of the left coronary artery were randomized to phenytoin group or operation control and compared to sham-operated rats.The time-dependent proteolytic activity of MMP-2 and MMP-9 were detected by gelatin zymography.Picrosirius red staining plus polarized light micrscopy was used for qualitative and quantitative analysis of collagen include collagen volume fraction(CVF) and ratio of typeⅠ/Ⅲ collagen.In addition,infarct thickness and myocyte cross-sectional area were also evaluated by image analysis.Results Fourteen days after myocardial infartion(MI),phenytoin reduced infarct wall thinning.Compared with control group,the rats received phenytoin had less myocyte cross-sectional area.Two weeks after MI,CVF in two groups both had significantly dynamic increase and phenytoin accelerated the change.In contrast to control group,ratio of typeⅠto type Ⅲ collagen in phenytoin increased more quickly.Apart from these results,phenytoin did little to CVF in non-infarcted region.Analysis of MMPs activity in myocardial extracts by zymography demonstrated that infarction-induced expression of proMMPs and active MMPs was both upregulated in phenytoin group and operation control.We found that after MI,MMP-9 activity increased as early as 1 day and reached a maximum then gradually descended,whereas MMP-2 began to increase rapidly and remain elevated for up to 14 days thereafter.Phenytoin seemed toenhance expression of MMP-2 and MMP-9.1 day after MI,active MMP-9 in phenytoin group expressed an increasing trendency compared to MI control.Conclusion Phenytoin can attenuate the degree of post-infarction left ventricular dilation and expansion of the infarct during the early phase of MI healing.MMP-2 and MMP-9 enhanced by phenytoin probably played a prominent role.

OBJECTIVE To investigate the impact on semen quality of Ureaplasma urealyticum(Uu) and Chlamydia trachomatis(Ct) infections.METHODS Uu and Ct in semen were detected and the samples of semen were analyzed for 228 cases in infertiliry group and 132 cases in fertility group,The semen quantity,sperm density,survival rate,vitality,liquefacient duration,normal morphologic rate,deformity rate and death rate were compared.RESULTS Among 228 cases in infertility group,173 were positive in Uu and Ct(75.9%),obviously higher than that of fertile males(28.0%).Sperm density,vitality,survival rate and normal morphologic rate in the infertility group were remarkably lower than those of the fertility group(P0.05).CONCLUSIONS The genital infection of Uu and Ct has its impact on the multiple parameters of semen,and semen quality as a whole is decreasing.