1.Pharmaceutical Care for One Case of Severe Brain Injury with Infection after Decompressive Craniotomy
China Pharmacist 2015;18(12):2126-2129
Objective:To explore the participation mode of clinical pharmacists in anti-infective therapy. Methods:Clinical phar-macists provided rational medication suggestions for anti-bacterial and antifungal therapy, and carried out pharmaceutical care for one patient with lung and intracranial infection after decompressive craniotomy for severe brain injury. Results: According to the sugges-tions of clinical pharmacists, fluconazole therapeutic regimen and amphotericin B therapeutic regimen was applied in turn respectively with the treatment course of 32d and 28d. During the treatment, the pharmacists also provided therapy advice on the infection induced by Staphylococcus epidermidis and Pseudomonas stutzeri, ADR warning and monitoring of fluconazole and amphotericin B, and adjust-ment of anti-infective therapy according to the results of antimicrobial susceptibility testing. The therapeutic effect was significant. Conclusion:The participation of clinical pharmacists in drug treatment can optimize medical therapy, which contributes to the coopera-tion between doctors and pharmacists.
2.Research Progress of Taking Key Enzyme in Metabolism as Selectable Marker in Transgenic Plant
Hui-Yong JIA ; Jia TIAN ; Jie LI ;
China Biotechnology 2006;0(03):-
In plant genetic engineering, selectable marker is needed to distinguish transformant. As the commercialization of transgenic plants, people are more and more paying close attention to their safety, among which mainly refers to the safety of selectable marker. In order to increase the safety of transgenic plants, biologists began to search for biosafe selectable marker.Current research progress of taking key enzyme in metabolic pathways were overviewed,which include glycometabolism, amino acid metabolism, hormone metabolism ,nucleotide metabolism and protein metabolism et cetera, as selectable marker in transgenic plants.
4.Study on Mutated DHDPS Gene as Selectable Marker of Transgenic Plant
Hui-Yong JIA ; Jia TIAN ; Pei-Qing LI ; Jie LI ;
China Biotechnology 2006;0(05):-
The rodipicolinate synthase gene was cloned from Nicotiana tabacum L.cv,which encodes the key enzyme in the synthesis of lysine,and then a vitro molecular reform reliefing feedback suppression was made. Some resistant seedlings were acquired using the mutated gene as selectable marker and analog of lysine as selectable reagent,and the detection results were positive by means of PCR and Real-Time PCR,however the phenotype is somewhat abnormal.
5.Clinical observation on eyelid herpes zoster with external use ganciclovir ophthalmic gel
Shu-Quan, LI ; Jia, JIA ; Zhi-Yong, ZHANG
International Eye Science 2014;(7):1356-1357
AlM: To observe the treatment efficacy of eyelid herpeszoster with external use ganciclovir ophthalmic gel.METHODS:A total of 77 patients were randomly divided into treatment group and control group. ln the treatment group, ganciclovir ophthalmic gel was topical coated on the basis of antiviral drugs, while the control group was coated with conventional acyclovir ointment and antiviral treatment.
RESULTS: The cure rate was 95% in the treatment group, and the control group was 83% after 10d treatment. The difference was significant.
CONCLUSlON: The efficacy of ganciclovir ophthalmic gel treated eyelid herpes zoster is obvious.
6.Application of prism alternate cover test and perimeter strabismometry in strabismic operation
International Eye Science 2015;(7):1290-1292
AlM:To discuss the application of prism alternate cover test ( PACT) and perimeter strabismometry in strabismic operation.METHODS: A total of 145 patients with concomitant esotropia or exotropia, aging 2 ~ 50 years old, were included. They were examined by PACT and perimeter strabismometry before operation. The strabismic operations were performed, the orthotropia rate of postoperation was assessed.RESULTS:Using PACT and perimeter strabismometry, there was a significantly difference of deviating angle ( P<0. 05) in mild and severe strabismic group. But there was no significantly difference of deviating angle (P>0. 05) in moderate strabismic group. The functional cure rate of postoperation was respectively 70. 83%, 67. 01%; the clinical cure rate was 20. 84%, 24. 74%; invalid rate was 8. 33%, 8. 25% .CONCLUSlON: The application of PACT and perimeter strabismometry in strabismic operation design, referring to the eye axial length, this can be helpful of improving the cure rate postoperation and decreasing the danger of reopreation.
7.Efficiency of Austrian Combi40+ Cochlear Implant In Deaf Children
Ziguang SHI ; Yong LI ; Yuezhi JIA
Journal of Medical Research 2006;0(05):-
Objective To discuss the effectiveness of Austrian Combi40+Cochlear Implant.Method Six cases of deaf children implanted Austrian Combi40+Cochlear Implant during the March,2004 to September,2005 were analyzed retrospectively.Result All cases were implanted successfully without complication.All deaf children acquired hearing of some degree when implant devices were operated in one month later.Also All cases were getting their speech intelligibility improved by hearing and speech rehabilitation.Conclusion The effectiveness of Austrian Combi40+Cochlear Implant is related to the age of deaf children with cochlear implant.The early deaf children get cochlear implant and hearing and speech rehabilitation,the better they have speech intelligibility.
8.Effects of different methods of volume therapy on inflammatory response in patients undergoing liver cancer resection
Chao LI ; Yong WANG ; Ruiqin LI ; Hemei WANG ; Huiqun JIA
Chinese Journal of Anesthesiology 2012;32(4):477-480
Objective To investigate the effects of different methods of volume therapy on the inflammatory response in patients undergoing liver cancer resection.Methods Forty ASA Ⅰ or Ⅱ patients,aged 40-60 yr,with body mass index 20-25 kg/m2,undergoing liver cancer resection,were randomly divided into 2 groups ( n =20 each):routine fluid replacement group (group Ⅰ ) and goal-directed fluid replacement group (group Ⅱ ).The fluid replacement regime in group Ⅰ =compensatory volume expansion (CVE) + physiological requirements + cumulative loss + confinued loss + the third space losses.CVE was replaced with lactated Ringer's (LR) solution 5 mg/kg before anesthesia induction.The physiological requirements and cumulative loss were replaced with LR solution according to the principle of 4-2-1.The continued loss equal to the intraoperative blood loss was replaced with the equal volume of 6% hydroxyethyl s tarch ( HES 130/0.4).The 3rd space losses were replaced with LR solution 5 ml·kg-1 ·h-1.In group Ⅱ,CVE was replaced with LR sol6ution as in group Ⅰ.LR solution was infused after anesthesia induction at 5 ml·kg-1 ·h-1.6% HES was infused to maintain left ventricular ejection time (LVETc) between 350-400 ms.When 350 ms < LVETc < 400 ms and the amplitude of stroke volume ( SV ) increased by > 10%,6% HES was infused continuously until the amplitude of SV increased by ≤ 10%.Blood samples were taken before anesthesia induction and at the end of operation for measurement of serum TNF-α,IL-2,IL-4,IL-6 and IL-8 concentrations.The adverse cardiovascular reactions were recorded.Results Compared with group Ⅰ,the serum TNF-α,IL-6,IL-8 concentrations were significantly decreased,the serum IL-2 and IL-4 concentrations were significantly increase,and the incidence of hypotension and tachycardia was significantly decreased in group Ⅱ ( P < 0.05).No adverse cardiovascular reactions were found in both groups.Conclusion LVETc and SV-guided volume therapy can maintain the blood volume and inhibit the inflammatory response and is suitable for the patients undergoing liver cancer resection.
9.Effect of drought stress on growth and physiological-biochemical characteristics of Stellaria dichotoma.
Duo-Yong LANG ; Jia-Jia CUI ; Zhou DA ; Yue-Tong LI ; Li ZHOU ; Xin-Hui ZHANG
China Journal of Chinese Materia Medica 2014;39(11):1995-1999
A pot experiment was conducted to study effect of drought stress on leaf physiological characteristics and growth of one year old Stellaria dichotoma seedlings. The result showed that plant height and shoot dry weight significantly decreased with decrease in soil water content; however, root length and root dry weight increased at light drought stress and decreased at severe drought stress. The result also showed that with the decrease of soil water content, proline content in S. dichotoma leaves decreased then increase, while solube protein content decreased. Activities of SOD and POD in S. dichotoma leaves significantly decreased as soil water content decreased, while activity of CAT significantly decreased at severe drought stress. Membrane permeability in S. dichotoma leaves increased, while MDA content decreased then increased as soil water decreased. These results suggest that S. dichotoma had osmotic stress resistance ability and reactive oxygen scavenging capacity at light drought stress, which caused S. dichotoma growth was no inhibited at a certain extent drought stress.
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10.Evaluation of predictive performance of propofol target-controlled infusion system incorporating the Schnider pharmacokinetic parameters
Chao LI ; Yong WANG ; Huiqun JIA ; Kunfeng GU
Chinese Journal of Anesthesiology 2011;31(4):407-409
Objective To evaluate the predictive performance of propofol target-controlled infusion (TCI) system incorporating the Schnider pharmacokinetic parameters in Chinese patients. Methods Forty ASA Ⅰ or Ⅱ patients, aged 25-45 yr, with body mass index 20-25 kg/m2 , scheduled for gynecological laparoscopic surgery un der general anesthesia, were enrolled in this study. Anesthesia was induced with TCI of propofol (target plasma concentration (Cp) 3 μg/ml) and remifentanil (Cp 4 ng/ml) . Propofol was infused by Orchestra TCI system incorporating the Schnider pharmacokinetic parameters. Tracheal intubation was facilitated with rocuronium 0.6 mg/kgafter the patients lost consciousness. The patients were mechanically ventilated. PETCO2 was maintained at 30-40 mm Hg. Anesthesia was maintained with TCI of remifentanil (Cp 4 ng/ml) and propofol (Cp 3-5 μg/ml) and intermittent iv boluses of atracurium 0.2 mg/kg. BIS value was maintained at 40-45. Venous blood samples were obtained at 15, 30, 45 and 60 min after pneumoperitoneum for measurement of blood propofol concentrations by high performance liquid chromatography with fluorescence detector. Performance error, median prediction performance error, median absolute performance error, wobble and divergence of propofol TCI system were calculated. Results The value for performance error was 21 % (13%), for median prediction performance error 6.7 % (37.4%),for median absolute performance error 19% (18%), for divergence - 0.65%/h (0.82%/h) and for wobble 16.3% (15.2% ) . Conclusion The accuracy of propofol TCI system incorporating the Schnider pharmacokinetic parameters is high in Chinese patients and its predictive performance is acceptable clinically.