The ISO9000 family standards developed by the international organization are the international quality standards which are widely used in various types and sizes of organizations.At present,the graduate student management method introduced by our country plays a certain positive role in guaranteeing the graduate students quality,but it still lacks effective quality control and has not formed the perfect quality management system.Through the analysis of the status quo of the development of graduate education and the problems need to pay attention to in the ISO9000 family standard procedure,document and other factors as well as the construction of the ISO9000 family standard,we put forward goal elaboration,regular staff training and other measures.

Objective To explore the effects of walking training assisted by electromyographic biofeedback (EMGBF) on the lower limb function of subacute stroke patients with foot drop. Methods Forty subacute stroke patients with foot drop were randomly divided into an EMGBF training group (n =20 ) and a routine rehabilitation training group ( n =20 ).Both groups received routine rehabilitation training.The EMGBF training group also received walking training assisted by three stages of EMGBF.The subjects were treated for 40 min twice per day,5 days a week for 6 weeks.Clinical and functional evaluations such as surface electromyogram (sEMG) signals from the anterior tibial muscle,active ankle dorsiflexion range of motion ( AROM ),scores on the modified Ashworth scale (MAS),results of the modified Lovett manual muscle test (mMMT),pace and step length,Berg balance scale (BBS) scores,Fugl-Meyer movement function scale (FMA) assessments,modified Barthel index (MBI) scores and Holden walking function test results were observed before treatment,after 6 weeks of treatment and at follow-up 6 weeks after the end of treatment. Results Before treatment there was no significant difference between the two groups.There were significant differences in all of the assessment results in the EMGBF training group after treatment compared with before treatment and compared with the routine rehabilitation training group after treatment.At followup 6 weeks after training,average pace (69.75 ± 35.09 m/min),step length (60.98 ± 14.09 cm),FMA score (32.5 ± 6.34 ) and MBI score (88.65 ± 14.13 ) in the EMGBF training group were all significantly better than in the routine rehabilitation group.At follow up,5 patients in the EMGBF training group achieved grade 5 in the Holden walking function test versus only 1 patient in the routine rehabilitation group. Conclusion Walking training assisted by EMGBF is effective in improving lower limb motor function in subacute stroke patients with foot drop.

Objective To develop medical equipment suited for medical care.Methods The secondary auxiliary wings were added inside the original intravenous infusion dressing clips on the left and right,and then a new stereo magnetic infusion dressing came into being with the medical 3M sticking membrane and glutinous magnetic woundplast.Results The Stereo Magnetic Intravenous Infusion Dressing was gifted with the functions of observing,supporting,and fixing the sting area.The model successfully passed the animal experiments.Conclusion The new stereo magnetic infusion dressing,easy to use,can satisfy the requirements of medical care.

Objective To investigate the effect and the underlying mechanism of miRNA (miR)-506 regulating transforming growth factor-β1 (TGF-β1)-induced endothelial-mesenchymal transition of human aortic endothelial cell lysates.Methods In this study,miR-506 and inhibitory members of the ASPP family (iASPP) were selected as the study objects.First,the expression of miR-506 in human aortic endothelial cell lysates (HAEC) lysate was detected under different concentrations of TGF-β1.The expression of platelet endothelial cell adhesion molecule-1 (CD31),endothelium-cadherin (VE-cadherin),fibroblastspecific protein-1 (FSP1),α-smooth muscle actin (α-SMA) and N-cadherin was determined after miR-506 mimics/mimic negative control (NC) transfection into HAEC treated with or without TGF-β1.Then the expression levels of iASPP in miRNA-506 mimics/mimic NC-transfected HAECs treated with or without TGF-β1 were determined.Finally,the protein expression of CD31,VE-cadherin,FSP1 and α-SMA in HAEC transfected with miR-506 mimics/mimic NC was determined after iASPP overexpression.Results TGF-β1 can induce human aortic endothelial cell mesenchymal transition,down-regulate CD31 and VE-cadherin expression while up-regulate FSP1,α-SMA and N-cadherin expression;TGF-β1 inhibited miR-506 expression,promoted iASPP expression;miR-506 transfection into HAECs up-regulated CD31 and VE-cadherin expression while up-regulate FSP1,α-SMA and N-cadherin expression;miR-506 could downregulate the expression of iASPP;the overexpression of iASPP increased the expression of FSP1,α-SMA and N-cadherin,down-regulated CD31 and VE-cadherin and promoted the transformation of human aortic endothelial cells.Conclusions The transfection of iASPP plasmid promoted the endothelial-mesenchymal transition of human aortic endothelial cell lysates.The transfection of miR-506 inhibited the expression of iASPP and inhibited the endothelial-mesenchymal transition of human aortic endothelial cell lysates induced by TGF-β1.

ObjectiveTo explore the change of ranges of abduction motion of hip joint in the simplified Taichi quan exercise.MethodsThe ranges of abduction motion of hip joint of 30 healthy male young subjects were measured using a goniometer.ResultsThe ranges of abduction motion of both hip joints had markedly exceeded their normal ranges of 0°-45°abduction motion during the exercise.ConclusionThe simplified Taichi quan can strengthen hip abductors to prevent and correct the deformity of hip adduction.

Objective To explore the value of contrast-enhanced ultrasonography (CEUS) in diagnosis of esophagogastric varices (EGV) compared with color Doppler flow imaging (CDFI). Methods CDFI and CEUS were performed on 23 variceal patients based on the B mode ultrasound examination. The blood flow of esophageal varices (EV) or gastric varices (GV) displayed with CDFI and CEUS were compared. Results The flow signal demonstration rate of EV with CDFI and CEUS was 42.11% (8/19) and 73.68% (14/19), while that of GV with CDFI and CEUS was 76.47% (13/17) and 94.12% (16/17), respectively. CEUS displayed the flow signal of EV better than CDFI did (χ~2=4.17, P＜0.05), but the flow signal demonstration rate of GV was not significantly different (χ~2=1.33, P＞0.05). Conclusion CEUS can be used more extensively in clinic practice for diagnosis of EGV.

Aim To investigate the effect of chlorogen-ic acid,caffeic acid,and ferulic acid on expression of molecules related with inflammatory response of HMECs induced by activated complement alternative pathway.Methods CVF was used to activate the al-ternative pathway of serum complement.After exposure of HMECs to activate complement for various times, supernatant of cell culture was removed and assayed for content of ICAM-1,IL-6,IL-8,t-PA,and PAI-1 u-sing ELISA kits.The expression of the above mole-cules induced by activated complement was measured after HMECs were pre-treated with 50,100,250 μmol ·L-1 of CGA,CA,and FA.Results After HMECs were exposed to the product of the activated comple-ment alternative pathway,the expression of ICAM-1 , IL-6,IL-8,t-PA,and PAI-1 was up-regulated.The expression of ICAM-1,IL-6,IL-8,t-PA,and PAI-1 was down-regulated by various concentrations of CGA, CA,and FA.ICAM-1 and IL-8 were inhibited most significantly in all molecules mentioned above.CA ex-hibited the best intervention effect,followed by FA. Conclusion Certain concentration of CGA,CA,and FA can inhibit the expression of ICAM-1,IL-6,IL-8, t-PA,and PAI-1 in HMECs induced by the activation of the alternative complement pathway,indicating that CGA,CA,and FA can inhibit inflammatory response of HMECs.

AIM: To explore the surgical methods and clinical effects on young patients with congenital lower eyelid entropion.
METHODS: There were 27 patients ( 45 cases ) who suffered congenital lower eyelid entropion accepted the modified blepharosphincterectomy. The clinical effects and complications were evaluated.
RESULTS: After followed up for 6mo, 42 eyes were fully recovered, 3 eyes were unsuccessful and the cure rate was 93%, 5 eyes suffered minor lower eyelid skin folds, none had lower eyelid retraction and ectropion.CONCLUSION: Modified blepharosphincterectomy is an ideal cosmetic surgical treatment for young patients with congenital lower eyelid entropion. It is an effective surgical treatment with fewer complications.

Fulminant myocarditis is one of the most common diseases causing sudden cardiac death in pediatrics and young adults.The pathophysiology of fulminant myocarditis involves both direct myocyte dysfunction and immune-mediated tissue injury,which result in severe damage in cardiac structure and func-tion.It always rapidly developed into cardiogenic shock in several hours.Despite the high mortality rate,if pa-tient response well to a series of progressive treatment,most of them would get complete resolution.Better understanding of the hemodynamic changes of fulminant myocarditis is the key point of rapid diagnosis and proper interventional treatment.In this article we made a summary introduction of those hemodynamic chan-ges in fulminant myocarditis.

Objective To compare the difference of MRI characteristics of pilomyxoid astrocytomas (PMA)and pilocytic astrocy-tomas (PA)in hypothalamus/optic chiasma region.Methods The MR images and clinical materials of 20 PMAs and 20 PAs proved by pathology were reviewed retrospectively.Imaging characteristics and clinical materials were analyzed,including age,gender, size,the signal characteristics of the tumor,growth,enhancement pattern,hydrocephalus and leptomeningeal dissemination.Results There were statistically significant differences in streaky low signal intensity among the high intensity of T2 WI and leptomeningeal dissemination between PMA and PA (P <0.05).However,no statistically significant differences were found in the other character-istics and clinical materials between the two types of tumors.Conclusion PMA and PA in hypothalamus/optic chiasma region are difficult to make differential diagnosis.The streaky low signal intensity among the high intensity of T2 WI and more frequent lepto-meningeal dissemination are helpful differential features of PMA as compared to PA.