Dens invaganatus is a type of abnormal morphological differentiation because of the involution in crown surface before tooth calci-fication,it is mostly found in maxillary lateral incisor teeth and rare in incisor.A case of a malformed lingual groove of left maxillary central incisor with 2 root canals is reported in the paper.

Traditional Chinese medicine exercise therapy has a long history in rehabilitation and keep in good health, which pays attention to integration of body and spirit.There are many clinical studies proving that it can improve heart function and quality of life in patients with cardiovascular diseases, and it is suitable for vigorously generalizing.

Objective To investigate the changes of molecule markers of pre-thrombotic state,expression of CD62P,concentration of plasma fibrinogen(Fib),prothrombin time(PT),activated partial thromboplastin time(APTT),thrombin time(TT) in the patients with essential hypertension for providing theoretical evidence for diagnosis and therapy of thrombus complication in early clinical stage.Methods Fifty-three patients with hypertension(grade Ⅰ 17 cases,grade Ⅱ 16 cases,grade Ⅲ 20 cases)were selected and twenty-eight people with medical examination in good health were used as control group.The expressions of CD62P and CD61 were measured by flow cytometry.The concentration of Fib and the levels of PT,APTT,TT were measured by Class coagulation method.Results The expression of CD62P and the concentration of plasma Fib in essential hypertension group were higher than those in control groups(P0.05).Conclusion The essential hypertensive patients are in pre-thrombotic state,the activation of platelet and blood clotting activity increase.Molecular markers should be measured in essential hypertensive patients for prevention and treatment of thrombotic disease.

OBJECTIVE To explore the infection situation of surgical operation and enhance postoperative(observation) and rational use of antibiotics.METHODS Totally 11 208 cases after surgical operation were(investigated)(retrospectively).The diagnosis standard was based on the Diagnosis Standard of Hospital(Infection)(Draft) published by Ministry of Health,the People′s Republic of China in Jan 2001. RESULTS There were(11 208) cases after surgical operation from Jan 1,2000 to Dec 31,2004 in our hospital and 275 cases suffered(incision)(infection,) there(infection) rate was 2.45%.The pathogenic bacteria of incision infection were confirmed as G~-(bacilli)(72 strains,78.26%),and G~+ cocci(20 strains,21.74%).CONCLUSIONS The key points of prevention of incision infection are sterile operation,protection of operation field when incising cavity organ,inside(sterilization),flushing of abdominal cavity and incision,change of contaminated apparatus,gloves and dressing promptly,(selection) of suture line and suture technique,besides the serious operation preparedness and skin(sterilization).

Objective To evaluate the capability of virtual non-contrast (VNC)of the second-generation dual-source CT (DSCT), and to display the adipose tissue of gastric serosa.Methods 48 patients with gastric cancer undergoing preoperative DSCT and sur-gery were enrolled.The true non-contrast (TNC)values and VNC values of perigastric adipose tissue were measured and compared with conventional non-contrast scanning and VNC of arterial phase and venous phase.The VNC values of perigastric adipose tissue were compared between serosal involvement group and non-serosal involvement group.Results There was statistical difference be-tween TNC values and VNC values of perigastric adipose tissue (P <0.05),the absolute differences were under 13 HU.The differ-ence of VNC values between serosal involvement group and non-serosal involvement group was significant (P <0.05).Conclusion VNC of the second-generation DSCT can clearly display the adipose tissue of gastric serosa.It has certain capability in judging sero-sal involvement.VNC has the proficiency to replace conventional non-contrast scanning.

Objective To investigate the depiction rate of normal cisterna chyli and thoracic duct by nonenhanced MR lymphography and to describe their appearances on MR imaging.Methods Special MR hydrography sequence was added to the MR imaging protocols of 112 patients undergoing MR examination of the thorax and upper abdomen.MR imaging sequences included:①Respiratory-gated HASTE T2W sequence;②Breath-hold FLASH T1W sequence;③Respiratory-gated TSE 3D T2W sequence(3D MR hydrography sequence)in coronal plane.One hundred cases who met the inclusion criteria were included into the study for observation of the depiction rate,location and morphology of cisterna chyli and thoracic duct.Results On TSE 3D T2W imaging:①Cisterna chyli was visualized in 71/100(depiction rate 71.0%),morphologically including single-tube type 43.7%(31/71),bifurcation type(2-3 tubes)23.9%(17/71),plexus type 32.4%(23/71).Average length of the cisterna chyli was 4.5 cm.②The depiction rate of the lower segment of thoracic duct was 57.0%(57/100),average ductal diameter was 0.23 cm.③The depiction rate of upper segment of the thoracic duct was 31.0%(31/100).Conclusion As a noninvasive method for depicting the lymphatic system,nonenhaced MR lymphography(TSE 3D T2W sequence)demonstrated a high depiction rate for cisterna chyli and lower thoracic duct.Combined with axial images of HASTE and FLASH sequences,the location and morphology of these larger lymphatic ducts can be defined.

Humans ; Hypertension ; complications ; Metabolic Syndrome ; complications ; physiopathology

Objective To study the apoptosis-inducing effect of Oridonin on PC-3 cells line and the role of Survivin in the process.Methods After PC-3 cells were incubated with different concentrations of Oridonin,cell viability was analyzed with MTT assay.The percentage of earlier apoptosis cell was analyzed by flow cytometry.The protein expression of Survivin in PC-3 cells were detected by Western blot and fluorescent quantitative PCR.Results Oridonin effectively inhibited the proliferation of PC-3 cells in a concentration-time dependent way.After PC-3 cells were treated with Oridonin ( 2.5,5,10,20,40 μmol/L)for 48 hours,the cytotoxicity index were 9.2％,25.3％,39.3％,77.2％,92.5％ and the IC50 of PC-3 cells was 10.29 μmol/L,respectively.Flow cytometry was used to detect the effect of different concentration of Oridonin (0,10,20,40 μmol/L) for 48 hours,the apoptotic rates of PC-3 cells were 4.8％,15.4％,19.5％,27.4％ ( P ＜ 0.05).Oridonin down-regulated Survivin protein in a concentration-dependent way in PC-3 cells.Conclusions Oridonin can induce the apoptosis of PC-3 cells by a concentration-dependent manner.Oridonin can induce the apoptosis of PC-3 cells by down-regulated Survivin protein.

MiR-494 involves in cell cycle regulation,differentiation and apoptosis processes of normal ceils.Recent study shows that abnormal expression of miR-494 is associated with oncogenesis closely,and it participates in the invasion and metastasis of tumor cells and so on.MiR-494 is not only a tumor suppressor gene,but also can be considered as a cancer-promoting gene.MiR-494 can regulate the oncogenesis and development of the tumor through a variety of target genes and signaling pathways.

Objectives:To explore the change of peripheral blood CD5+B cells and its association with indexes of liver function in patients with hepatitis B.Methods:Change of peripheral blood CD5+B cells was detected immunohistochemically.The indexes of liver function were examined by HITAI 7170 automatic analyzer.Results:There were no significant differences in the percentage of peripheral blood CD5+B cells between patients with acute hepatitis B and normal controls.With the severity development of hepatitis B from chronic hepatitis to cirrhosis,the percentage of peripheral blood CD5+B cells is positively correlated with the increase of ALT，AST，GGT，TB and ALB.Conclusions:A large number of CD5+B cells are activated in patients with chronic hepatitis B,heavy chronic hepatitis or cirrhosis.The percentage of peripheral blood CD5+B cells increases concomitantly with the liver impairment.