Acupuncture Therapy ; instrumentation ; Adolescent ; Adult ; Aged ; Facial Paralysis ; therapy ; Female ; Humans ; Male ; Middle Aged ; Young Adult

Objective To investigate the effect of pre injection of penehyclidine hydrochloride on the airway gland secretion of children with laparoscopic inguinal hernia surgery under general anesthesia.Methods 72 cases of children undergoing laparoscopic inguinal hernia surgery under general anesthesia in pediatric surgery department in our hospital were selected and divided into two groups,grouped by random number table method,control group of 36 cases were treated with preoperative injection of atropine,the experiment group of 36 cases were treated with preoperative injection of penehyclidine hydrochloride.Heart rate on pre injection(T0),before induction of anesthesia(T1),five min after induction(T2),ten min after induction(T3)and extubation(T4),the airway gland secretion,respiratory function related indexes,and the incidence of adverse reactions were compared.Results Compared with the control group,re fixed rate of tracheal tube during operation and the incidence of choking tube at T4 time were lower,the levels of PIP、Raw were lower and the level of CL was higher on five min after intubation,the HR at T1,T2,T3,T4 time was lower,with statistical significance(P<0.05);the incidence of adverse reactions in the control group 30.56％was higher than the experiment group 11.11％,with statistical significance(P<0.05).Conclusion Pre injection of penehyclidine hydrochloride can inhibit airway gland secretion in children with laparoscopic inguinal hernia surgery under general anesthesia,protect the respiratory function,and does not increase heart rate,the safety was higher.

Bacterial Infections ; drug therapy ; metabolism ; Biomarkers ; analysis ; Clinical Trials as Topic ; Humans ; Infant, Newborn ; Neonatology ; Research Report

This study was aimed to find the optimal conditions for seed propagation of Fritillaria cirrhosa in the plateau region in order to summarize the best sowing time and method of artificial propagation. Seeds were stored in appropriate environment. After the seeds matured, the seedbed of F. cirrhosa was treated with cattle dung humus and small shed. The results showed that early March is the best sowing time and cattle dung humus with a thickness about 1 cm is the best coving for propagation of F. cirrhosa; the growing period of film propagation (FP) is about 160 days each year compared with that of control about 50-60 days, respectively. It was concluded that the propagating seeds in plastic greenhouses by using cattle dung humus as planting substrates, using sunshade nets for shading and keeping humidity by spraying can effectively prolong the growing period, improve the retention rate of annual bulbs and the production of F. cirrhosa.

China ; Conservation of Natural Resources ; Electricity

Objective To investigate the distribution and effect of class Ⅰ integron in 40 strains of clinical multi-resistant Enterobacter cloacae. Methods Kirby-Bauer method was used to analyze the drug resistance of 40 strains of multi-resistant Enterobacter cloacae and the drug resistance gene was analyzed by polymerase chain reaction (PCR). Results 28 strains (70.0%) of 40 clinical isolates were observed to contain class Ⅰ integron. Drug resistance test showed their resistance rate to 10 kind of an-tibaeterials as follow: 7.5% to imipenem, 5.0% to meropenem, 75.0% to piperacillin-tazobactam,55.0% to amikacin, 12.5% to cefepime, 52.5% to ceftazidime, 55.0% to cefotaxime, 90.0% to ceftriaxone;81.8% to ticarcillin-clavutanic acid, and 85.0% to ciprofloxacin. Conclusion This study shows that the resistance situation of Enterobacter cloacae isolates is very serious, and class Ⅰ integron may play an important role in the muhidrug resistant mechanism.

BackgroundThe neutrophils infiltration and vascular endothelium damage are found in the patients with diabetic retinopathy (DR).Calprotectin existes in the cytosol outside lysoome.It is thought to be a marker of inflammation.The effect of calprotectin in the development of DR is still in the study. Objective This study was to investigate the contents of plasma calprotectin in different stages of DR in patients with type 2 diabetes mellitus. Methods This was a case-control study.Sixty consecutive patients with type 2 diabetes mellitus were enrolled in this study.The patients were assigned to non-DR (NDR) group,non-proliferative DR (NPDR) group and proliferative DR (PDR)group according to fundus appearance and fundus fluorescein angiography(FFA) manifestation and 20 patients for each group.Twenty healthy subjects matched in gender,age and blood biochemical indicators were collected as the normal control group.The periphery blood samples were collected from the subjects for the detection of plasma calprotectin by ELISA.The plasma calprotectin levels were compared among different stages of DR and normal subjects.All subjects had signed informed consents.Results The contents of plasma calprotectin were (57.70±12.29 ),( 72.07± 10.14 ),( 87.70 ± 10.37 ),( 94.36 ± 9.40 ) ng/L in the normal control group,NDR group,NPDR group,PDR group respectively,with a statistically significant difference among 4 groups (F =73.09,P＜0.001 ).The content of calprotectin in PDR group showed a highest value in comparison with normal control group,NDR group and PDR group(q =20.157,10.648,4.497,P＜0.01 ).The content of calprotectin in NPDR group was significantly higher than that in NDR group( q=6.216,P＜0.01 ). ConclusionsPlasma calprotectin may play a role during the development of DR in type 2 diabetes mellitus patient.

Objective To analyze and discuss the effect of heat preservation on the recovery time of BIS and the concentration of propofol in the recovery period of the elderly patients with general anesthesia in order to provide guidance for clinical treatment.Methods 78 elderly patients with general anesthesia were selected as the subjects, and the patients were divided into the control group and the study group with 39 cases in each group.The patients were divided into the control group and the study group.Control group, only to be liquid input and covered by drapes, research group take liquid input and peritoneal flushing fluid heating and heating blanket coverage of insulation measures, were recorded and compared between the two groups from anesthesia began to operation to complete the different time points of esophageal temperature and MAP, and to observe the recovery time of patients and effect compartment concentration differences.Results Two groups at T0, T1 esophageal temperature no significant difference, study group T2-T6 esophageal temperatures were significantly higher than those of the control group (P<0.05), maps of the other two groups at different time points had no significant difference;when two groups of patients with propofol withdrawal BIS values had no significant difference, and in the time of discontinuation BIS is more than or equal to 80 recovery time and effect compartment concentration have significant difference (P<0.05).Conclusion The surgical treatment of elderly patients with general anesthesia can help maintain the body temperature, shorten the recovery time of BIS and reduce the concentration of propofol in the recovery period.

Objective To study the impact of pregnancy on differentiated thyroid cancer. Methods Retrospective comparative analysis were made between pregnancy-related thyroid cancer patients ( including nine months before delivery and one year after delivery) and the age-matched non-pregnancy-related thyroid cancer patients treated in the Department of head and neck, Fudan University Shanghai Cancer Center. Results There were 125 cases of pregnancy-related thyroid cancer patients from 1998 to 2008, including 28 cases (22.4%) of neck recurrence and metastasis after the surgery of thyroid cancer, meanwhile there were 691 cases of the agematched non-pregnancy thyroid cancer patients, including 90 cases ( 13% ) of neck recurrence and metastasis after the surgery of thyroid cancer. The rate of neck recurrence and metastasis after the surgery of thyroid cancer in the pregnancy-related group was higher than that in the non-pregnancy related group ( P ＜ 0.05). The maximum diameter of tumor in the pregnancy-related group and the non-pregnancy group is 2. 1 cm and 2.6cm respectively.There were 19 cases of multi-focal cancer ( 19.6% ) in the pregnancy-related group and 58 cases (9. 6% ) in the non-pregnant group, P ＜0.05. There were 23 patients (23.7%) of extrathyroid invasion in the pregnancy-related group and 65 patients ( 10.8% ) in the non-pregnant group, P ＜0.05. There were 67 cases (53.6%) and 35 cases (28%) of the central and lateral cervical lymph node metastasis in the pregnancy-related group respectively and 296 cases (42.8%) and 117 cases (16. 9% )in the non-pregnant group, P ＜0.05. There were 19 cases (15.2%) of extralymph invasion in the pregnancy-related group and 32 cases (4.6%) in the non-pregnant group, P ＜ 0.05. Conclusions The incidence of Extrathyroid invasion, multi-focal cancer, cervieal lymph node metastasis and extralymph invasion of the differentiated thyroid cancer is higher in pregnancy-related patients.Pregnancy may deteriorate the prognosis of thyroid cancer. For patients after the surgery of thyroid cancer, pregnancy may increase the risk of neck recurrence and metastasis. However, pregnancy does not affect the stage of thyroid cancer and the prognosis is still good.

Eukaryotic expression vector containing PTEN gene was transfected into SHG44 cell under mediation of lipofectamine, and positive cell clones were selected and amplified. Expression of PTEN gene was detected by in situ hybridization and immunohistochemistry. Flow cytometry, electron microscope and other methods were adopted to measure the effects of PTEN on cell cycle, ultrastructure, and cell growth of the transfected glioma cells, as well as on tumorigenicity in nude mice. The results showed that PTEN gene and protein were expressed in the glioma cells transfected with PTEN gene. The progression of cell cycle was arrested from G1 to S phase. The growth of cells transfected with PTEN gene in nude mice was dramatically inhibited compared with the parent SHG44 cells. This experiment suggested that the growth of SHG44 glioma cells could be arrested at G1/S phase, and it could be significantly suppressed by exogenous PTEN gene.