] Objective To investigate the effects of midazolam on vascular capacitance during hypovolemic shock in an animal model and the underlying mechanism. Methods Thirty male SD rats weighing 350-450 g were randomly divided into four groups : (1) control group (n = 8);(2) sympathetic nervous system block ( SNSB) group received hexamethonium 10 mg? kg-1(n = 8);(3) SNSB + noradrenaline (NA) group in which NA was infused following hexamethonium until BP returned to baseline (n = 9); (4) hypovolemia group in which 20 ml? kg-1 of blood was withdrawn ( n = 5). The animals were anesthetized with intraperitoneal ketamine. Spontaneous breathing was maintained. Femoral artery and vein were cannulated for MAP and CVP monitoring. Mean circulatory filling pressure (Pmcf) was measured. Midazolam 0.1, 0.3, 0.5 and 1.0mg?kg-1 were given iv successively with an interval of at least 20 min between two injections. MAP, CVP, HR and Pmcf were measured and recorded before and 2 min after each midazolam injection. Results In control, SNSB + NA and hypovolemia groups (group 1, 3, 4) midazolam significantly decreased MAP as compared with that before midazolam ( P

This study was to investigate the role of urethral catheter balloon dilatation(F16)in the treatment of intrauterine adhesion after hysteroscopic surgery.A total of 523 patients with severe intrauterine adhesion(IUA)following hysteroscopic surgery underwent urethral catheter balloon dilatation during April 2000 and December 2005.During 3～15 months'follow-up,normal menses were seen in 340 patients (65.0％),nearly normal menses in 165 patients(31.5％),and menoschesis in 18 patients(3.4％).302 patients showed normal uterine cavity with no recurrent endometrial adhesion,203 patients(38.8％)were found slight IUA,and only 18 patients(3.4％)still presented with severe adhesion.No operative complication was reposed.and the pregnant rate was 47.6％.In summary.urethral catheter balloon dilatation.based therapeutic strategies is safe.effective and beneficial.

Objective:Making single chain antibody(scFv)-alkaline phosphatase(Ap)fusion protein.Methods:An expression vector pSTE2-C66-Ap was constructed by sequentially inserting the Ap coding region into plasmid pSTE2-8E5 and replacing the VH-VL fragment of 8E5 by VH-VL fragment of C66.The fusion protein scFvC66-Ap was expressed in E.coli.and analysed by SDS-PAGE and immunoblotting.Results:Have obtained the scFvC66-Ap fusion protein with a molecular weight of 75 kD.It bound a 60 kD molecule from KG1a cell proteins on immunoblotting membrane detected directly by Ap enzymatic activity.Conclusion:A method permits the production of scFv-Ap conjugates in E.coli.which can replace conventionally prepared Ap-labeled antibodies in immunoassays.

BACKGROUND:How does the peripheral nerve block work to inhibit the functions? What effects does it do to the spinal cord and the cerebral center? How does it regulate the peripheral nerve and how does it change anatomy of brain center? Al above are stil unknown.
OBJECTIVE:To observe the effect of peripheral nerve block on the biology of spinal cord nerve cel s.
METHODS: Sixty New Zealand rabbits were selected and randomly divided into three groups: ischiadic nerve block group, subarachnoid block group and extradural nerve block group. Each group had 20 rabbits which were sub-divided into experimental group and control group with 10 rabbits in each subgroup. The rabbits in the three experimental groups were injected with lidocaine, bupivacaine and lidocaine at the middle point between femoral head and ischiadic tuberosity in ischiadic nerve block group, subarachnoid block group and extradural nerve block group, respectively. The rabbits in each control group were injected with normal saline at the same position.
RESULTS AND CONCLUSION:After ischiadic nerve block, extradural nerve block or subarachnoid block for 30 minutes, the Nigeria’s bodies of the little round cel s in the poliomyelia posterior horn laminaeⅢandⅣand the polyhedral cel s in the anterior horn laminaeⅨwere less than those in the control groups. Nuclei leaned towards one side. c-Fos protein expression was decreased or showed no expression, suggesting that the cord nerve cel function of corresponding spinal segments was inhibited. The spinal pia mater of spinal cord had a hierarchical or fracture phenomenon after subarachnoid block.

Objective To observe the change of homocysteine(Hcy)and lipoprotein-a[Lp(a)]of cerebral infarction patients and perform the correlation analysis on them.Methods The serum Hcy and Lp(a)levels were detected in 230 patients with cerebral in-farction and 90 healthy controls.The detection results were performed the comparison between the groups and the correlation analy-sis.Results The serum Hcy and Lp(a)levels in the overall cerebral infarction group and the cerebral infarction group with in-creased Hcy were significantly higher than those in the healthy controls(P <0.01).The serum Hcy and Lp(a)levels in the cerebral infarction group without increased serum Hcy had no statistical difference compared with the healthy control group(P >0.05).The serum Hcy and Lp(a)levels in the overall cerebral infarction group had no statistical difference compared with the cerebral infarc-tion group with increased Hcy(P >0.05).The serum Hcy and Lp(a)levels in the overall cerebral infarction group and the cerebral infarction group with increased Hcy had statistical difference compared with the cerebral infarction group without increased Hcy (P <0.01).The serum Hcy in the overall cerebral infarction group is positively correlated with Lp(a)(r =0.859,P <0.01).Con-clusion The increase of serum Hcy and Lp(a)levels is closely related with the occurrence of cerebral infarction and has certain val-ue in the diagnosis of cerebral infarction,moreover serum Hcy is positively correlated with Lp(a).

ObjectiveTo observe the clinical efficacy of acupoint application withBaoShen cataplasm in treating chronic kidney diseases (CKD) and its effect on the quality of life.MethodSixty CKD patients were randomized into a treatment group and control group, 30 cases in each group. The control group was intervened by conventional Western medicine, while the treatment group was by acupoint application withBaoshen cataplasm in addition to the same Western medicine treatment. The SF-36, symptom score and Chinese medicine, 24hupq, Scr, and GFR were observed before and after intervention.Result The total effective rate was 86.7% in the treatment group versus 66.7% in the control group, and the difference was statistically significant (P<0.05); in the treatment group, the effective rates for the kidney yang deficiency and kidney yin deficiency were respectively 88.2% and 84.6%, versus 68.8% and 64.3% in the control group, and the differences were statistically significant (P<0.05); the symptom score of Chinese medicine dropped significantly in both groups after intervention (P<0.05), while the change in the treatment group was more marked than that in the control group (P<0.05); after intervention, the Scr and 24hupq decreased and GFR increased significantly in both groups (P<0.05); the bodily pain, general health, vitality, social function, emotional role and mental health scored higher after intervention in both groups, and the scores in the treatment group were significantly higher than that in the control group (P<0.05).ConclusionAcupoint Application withBaoshen cataplasm can significantly improve the renal function and symptoms of CKD patients, promote the quality of life, and it can produce a content therapeutic efficacy in treating CKD due to kidney yang deficiency and kidney yin deficiency based on the conventional treatment.

Objective To detect serum leucine aminopeptidase (LAP) level in the patients with hyperthyroidism for investiga‐ting the changes of LAP in the patients with thyroid dysfunction and its clinical significance .Methods 117 patients with hyperthy‐roidism were taken as the test group and 109 healthy people as the control group .The differences of various laboratory indexes were comparatively analyzed and the correlation between LAP with FT 3 ,FT4 and TSH was analyzed .117 patients with hyperthyroidism were divided into the liver damage group(52 cases) and non‐liver damage group(65 cases) and performed the LAP determination . Results The serum LAP ,FT3 ,FT4 and TSH levels in the hyperthyroidism whole group ,hyperthyroidism liver damage group and hyperthyroidism non‐liver damage group had statistically significant differences compared with those in the control group (P<0 .01) ,serum LAP was positively correlated with FT 3 and FT4 ,and negatively correlated with TSH ;LAP ,FT3 ,FT4 and TSH had no statistical differences between the hyperthyroidism live damage group and the hyperthyroidism non‐liver damage group (P>0 .05) .Conclusion The serum LAP content is closely related with the thyroid hormones and has certain clinical reference value for the diagnosis ,disease condition evaluation and medication .Lap can serve as an independent diagnostic indicator .

Objective To evaluate the clinical value of hysteroscopy before in vitro fertilization and embryo transfer(IVF-ET).Methods From December 2003 to May 2005,130 patients received hysteroscopy in our hospital because of IVF-ET failure.Their intrauterine diseases were treated by hysteroscopy,and the abnormal tissues were examined pathologically.Results Among the 130 cases,87 had intrauterine diseases(87/130,66.9%) including endometritis in 40(40/130,30.8%),endometrial polyps in 23(23/130,17.7%),intrauterine adhesion in 20(20/130,15.4%),and submucous leiomyoma in 4(4/130,3.1%).In these patients,67 underwent IVF/ICSI or frozen embryo transfer within one year after the hysteroscopy,of which 20 patients achieved pregnancy.The clinical pregnancy rate was about 30%.The other 20 cases did not repeat IVF-ET in 10-20 months after the hysteroscopy,and had no pregnancy without contraception.Conclusion Pre-transfer hysteroscopy is helpful to avoid IVF-ET failure owing to intrauterine diseases.

Objective To observe the curative effects of Ginkgo dipyridolum injection in the treatment of acute cerebral infarction and its influence on blood rheology and fibrinogen content.Methods 96 patients with acute cerebral infarction were randomly divided into two groups.39 patients in Ginkgo dipyridolum group were treated with 20 ml Ginkgo dipyridolum injection plus 500 ml saline intravenously,once a day.57 patients in control group were treated with Xuesaitong injection 10 ml plus 500 ml saline intravenously,once a day.All treatment lasted for 14 days.The score of neurological deficit was evaluated to assess the clinical efficiency,the changes of indexes of blood rheology,fibrinogen content and skull CT in the two groups were observed before and after treatment.Results Total effective rate(94.9%)in the Ginkgo dipyridolum group was signifcantly higher than that in the control group(78.9%)(P

Objective To investigate the condition of inflammation and prethrombotic state (PTS) of the aged patients with coronary heart disease(CHD) complicated with esstential hypertension(EH) and type 2 diabetes mellitns(T2DM). Methods The levds of fasting plasma hs-CRP,D-D and VWF were all measured in 86 CHD patients with EH,84 patients with T2DM,80 patients with EH and T2DM,91 CHD patients and 79 normal cases. Results Compared with normal group,diseases groups had significantly higher hs-CRP,D-D,and VWF,but compared with CHD with EH and T2DM group,other diseases groups had lower hs-CRP,D-D and VWF(P <0.05) ;The concentration of serum hs-CRP was positively correlated with D-D and VWF in CHD group (P < 0. 05). Conclusion The inflammation and hypercoagulable state is more significant in CHD patients with EH and T2DM,thns the levels of plasma hs-CRP,D-D and VWF play an important role in the treatment.