Objective To research into the relationship between heart rate variability (HRV) and QT dispersion(QTd) in patients with type 2 diabetes mellitus(T2DM) complicated with coronary heart disease(CHD).Methods In the research 30 patients with type 2 diabetes mellitus complicated with CHD were classified as group A,33 patients with T2DM as group B and 21 normal healthy persons as controlgroup,group C.24-hour time domain and 24-hour frequency domain parameters of HRV were recorded by holter ECG and analysed through computer.A standard 12-lead synchronous electrocardiogram was used to measure QT interval and QTd.Results (1)The research showed that all parameters of HRV in group A were less than those in group B and group C (P

Objective To investigate the clinical efficacy and safety of low-dose rituximab (RTX) for patients in primary Sj(..o)gren's syndrome (pSS) with thrombocytopenia.Methods Four pSS patients,2 with refractory thrombocytopenia and 2 with glucocorticoid-dependent thrombocytopenia,were treated with rituximab at 100 mg,intravenous,weekly for a total of two cycles,together with prednisone 1-2 mg · kg-1 ·d-1,and the counts of platelets and B-cells were evaluated.Results Efficacy of treatment was observed in all patients.The counts of platelets,at (3-39) x 109/L baseline,incleased in 1-2 weeks,and went up to ( 107-241 ) × 109/L in 3-8 weeks.Sustained remission had been achieved for 27-52 weeks.The doses of prednisone were tappered to 3.75-7.50 mg/day in 12 weeks.One patient who relapsed at the 27th week (platelet count 47 x 109/L),was retreated with 100 mg of RTX and still had good efficacy.The counts of B-cells reduced to (0.007-0.010) × 109/L,but they did not achieved the depletion.There were no severe adverse events during RTX therapy.Conclusions Our study has shown good efficacy and tolerability of lowdose RTX for pSS with thrombocytopenia.Low-dose RTX allows for reduction in corticosteroid doses and B-cells,while large-scale randomized double-blind controlled trials are needed to confirm the results.

Objective To evaluate the concentration of urinary cadmium and serum sex hormone in cadmium-exposed male workers and explore the affecting factors and related mechanism.Methods The individuals exposed to cadmium from three workshops 40 in each, in a cadmium rechargeable battery manufacture plant were selected as the exposure groups according to concentrations of cadmium oxide in the workplace in November 2008, namely assembly, charging and butt-weld workshop for low, moderate and high dosage group.Another forty male workers without cadmium exposure of the same plant were selected as the control group.Urinary cadmium, serum testosterone,FSH and LH concentrations were measured and urinary cadmium concentration abnormity rate was calculated for the following analysis.Results Compared with the control group, the urinary cadmium concentration and its abnormity rate of the low and high dosage groups were all significantly increased(P9 years groups were all significantly increased(P

Objectives To evaluate methods to diagnose Budd-Chiari syndrome with inferior vena cava (IVC) thrombosis.Methods 31 cases underwent phlebography, 28 cases received color Doppler sonography preoperatively. Radical thrombectomy was carried out in 28 cases. Results IVC thrombi was found by phlebography in 24 cases, and by color Doppler sonograhy in 25 cases. There was no intraoperative death, nor acute pulmonary embolism.All patients were discharged uneventfully. Follow-up of 6 to 80 months found recurrence in 3 cases.Conclusions Large or lateral thrombi can be easily diagnosed by phlebography. Fresh or floating thrombi often missed by phlebography could be rushed out by blood stream at the time IVC was incised.The thrombi adhered to the IVC wall could be taken out with large balloon catheter or manually.

Objective To investigate the relation of the expressions of matrix metalloproteinase-2(MMP-2)and-9(MMP-9)with polymyositis/dermatomyositis(PM/DM).Methods Blood samples were obtained from 8 patients with PM,12 patients with DM and 10 normal human controls.Real-time quantitative RT-PCR and Western-blot were applied to examine the mRNA and protein expressions of MMP-2 and MMP-9 respectively in the peripheral blood mononuclear cells(PBMC)of these patients.Results The mRNA expression levels of MMP-2 and MMP-9 in the PBMC of patients with PM or DM were 2.41 and 1.66 folds as high as those of the normal controls,respectively;these difierences were significant between the two groups.Increased expressions of MMP-2 and MMP-9 were also noted in the patients with PM or DM.Conclusion MMP-2 and MMP-9 may play a part in the development of polymyositis and dermatomyositis.

Objective To evaluate left ventricular function in patients with acute myocardial infarction and to investigate the clinical value by two-dimensional strain imaging of ultrasound.Methods Study population was consisted of 28 patients with acute myocardial infarction of wall anterior and 30 healthy subjects.Images were recorded from the left ventricular the apical 4-chamber view,apical 3-chamber view,apical 2-chamber view respectively.Two-dimensional strain and strain rate were measured in the left ventricular segments using two-dimensional strain software.We analyzed that relationship between two-dimensional the global peak systolic strain,global strain rate and left ventricular end systolic volume(LVESV),left ventricular end diastolic volume(LVEDV),wall motion score index(WMSI),left ventricular ejection fraction(LVEF).Results ①Infarct segments in myocardial infarction group showed significant reduction of peak systolic strain as compared with normal group(P

Objective To explore the changes of CRP,IL-6,and IL-10 levels after laparoscopic or open repair of inguinal hernia in children.Methods Fifty children with inguinal hernia were randomly assigned to laparoscopic repair or open surgery groups(25 in each).In the laparoscopic group(LS group),the hernia sac was closed by ligating the inner ring,while in the open surgery group(OS group),high ligation was performed.The peripheral blood samples of the two groups were collected one day before,immediately after,and one day after the operations.ELISA was used to detect the levels of CRP,IL-6,and IL-10.Results In both the groups,the levels of CRP,IL-6,and IL-10 were significantly increased immediately after the surgeries(LS Group:q=8.508,11.307,and 22.111,P0.05),while in the OS group,the levels remained significantly higher(q=3.845,10.599,and 11.379,P

Objective To study the effect mechanism of neuroprotection of Sinomenine(Sin) on rats with cerebral ischemia-reperfusion(IR).Methods 80 Wistar rats were randomly divided into sham-operated group,IR group,Sin high-dose(60 mg/kg)group and Sin low-dose(30 mg/kg)group.The correlative dose of Sin were intraperitoneal injected in Sin high-dose and low-dose groups,respectively.30 min later,the IR models were made.After 24 h of IR,the volume of cerebral infarction(CI) and the change of cerebral pathology were observed by TTC and HE staining.The brain water content was investigated by dry and wet weight method.The expression of nuclear factor (NF)-?Bp65,intercellular adhesion molecule (ICAM)-1 and the activity of myeloperoxidase (MPO) in the frontal and parietal cortex were detected by immunohistochemistry.Results Compared with IR group,the ischemic impairment in Sin high and low-dose groups were obviously lighter,even lighter in Sin high-dose group;the CI volumes and brain water content were significantly reduced;while,even lower in Sin high-dose group(all P

Objective To analyze the mechanism of pigment epithelium?derived factor(PEDF)regulation in lung cancer cell proliferation and migration by vascular endothelial growth factor(VEGF). Methods VEGF expression was observed after overexpression or silencing of PEDF. Proliferation and migration were analyzed by MTT and transwell assays. Real?time PCR and Western blotting were performed to investigate the mechanism underlying PEDF regulation of the VEGF/SRC/FAK pathways. Results PEDF could inhibit the proliferation and migration of A549 cells by VEGF. Conclusion PEDF can be considered as a potential therapeutic target for lung cancer.

Elements, including experimental personnel, facili-ties, equipment, apparatus, technology and skills, are essential to carrying out pre-clinical reproductive toxicity research. As to a study , authenticity , normativity , scientificity and innovativeness guarantee the success. These four elements are independent of each other,but mutually supported. Authenticity and scientificity are the footstone and soul, respectively, for drug non-clinical re-productive toxicity research. Normativity guarantees authenticity and scientificity, and innovativeness relies on authenticity. Nor-mativity and scientificity ensure the reliability and dynamics of experimental results which perhaps is an effective way accessing innovation.