Objective To observe the effect of aqueous extract of Broussonetia papyrifera ( L. ) Vent on the ability of space learning and memory in the rats with Alzheimer' s disease (AD) induced by Aβ 25-35 and Dgalactose and to explore the mechanisms underlying those improvements. Methods The animal model of AD was established by Aβ 25-35 stereotactic injection into the hippocampus of rats in 5 minutes,while long-term intraperitoneal injection with D-gal. After the injection of Aβ25-35,rats were treated with aqueous extract of Broussonetia papyrifera ( L. ) Vent for the next 30 days. Morris water maze with computer system and the spatial exploration experiments were used to assess the behavior performances of the rats. Immunohistochemical technique was used to detect the expression of BiP, PERK and CHOP. Results The ability of space learning and memory of rats complex model of Alzheimer's disease induced by Aβ25-35 and D-gal was damaged,while escape latency was (20.90± 9.16 ) s,and the proportion of original platform quadrant was ( 11.05 ± 4.43 ) %. The expression level of Bip was reduced ,while the mean gray was ( 139.71 ± 3.47 ). The expression level of PERK and CHOP was increased,while the mean gray were (97.96 ± 5.97 ), ( 110.93±4.91 )separately. The escape latency of rats in the aqueous extract of Broussonetia papyrifera ( L. ) Vent treated groups was ( 5.41 ± 3.47 ) s and shorter than the model group,while the proportion of original platform quadrant was (48.28 ± 7.03 )% and higher than the model group.The expression level of Bip in the treated group was higher than the model group, while the mean gray were ( 121.17 ±4.76). The expression level of PERK and CHOP in the treated group was lower than the model group,while the mean gray were ( 122.11 ± 4.73 ), ( 123.34 ± 7.73 ) separately. Significant differences were observed between model group and aqueous extract of Broussonetia papyrifera ( L. ) Vent treated groups (P＜ 0. 05 ～ 0. 01 ).Conclusion Aqueous extract of Broussonetia papyrifera ( L. ) Vent can improve learning and memory disorders of the model rats induced by Aβ25-35 and D-galactose. ER (endoplasmic reticulum) stress and correlated apoptosis pathway might be involved in the underlying mechanisms.

Objective To investigate the management of prevention and control of catheter-related bloodstream in-fection (CRBSI)in intensive care units (ICUs)of tertiary hospitals in a city,and explore stategies.Methods Data about CRBSI prevention and control as well as quality management were collected and surveyed by self-developed questionnaires.Results Of ICUs in 8 hospitals,the incidence of CRBSI was 4.81 ‰,case infection rate was 5.02‰.6 (75.00%)hospitals had standard operating instruction for the prevention and control of CRBSI, 2 (25.00%)hospitals had maintenance record on central venous catheter,and 2(25.00%)hospitals implemented maximum sterile barrier and catheterization combination strategy,none hospital adopted anti-infective dressing and chlorhexidine bathing.Conclusion There are many problems in the standard operating instruction and implementa-tion of measures for the prevention and control of CRBSI in ICUs of tertiary hospitals in this city,which needs to be improved.

Diclofenac potassium delayed-sustained release pellets were prepared by double-layer coating method with ethylcellulose aqueous dispersion.The effects of release condition and pellet compositions on the in vitro drug release were evaluated.The formulation was optimized by the central composite design-response surface methodology.It was shown that the pH of the media greatly affected the in vitro drug release of the pellets while the viscosity of the media had little influence.Drug release from the pellets was related to the proportion of the inner coat to the outer coat and the amount of pore forming agent in the outer coat.The optimization of the formulation could be achieved by the central composite design-response surface methodology.

Objective To investigate the effects of pseudolaric acid B(PAB) on the invasiveness and metastatic activity of cervical cancer HeLa cells in vitro and the possible mechanism.Methods HeLa cells were divided into 5 groups: blank control group,DDP 3.125?g/ml group,20?mol/L PAB group,10?mol/L PAB group and 5?mol/L PAB group.The effect of PAB on invasive ability of HeLa cells was observed with a Transwell cell culture chamber.The mRNA expression of MMP22 and MMP29 in HeLa cells was detected by RT-PCR and the protein expression was detected by Western blotting.Results The average number of invaded HeLa cells was 15.60?2.45 in 20?mol/L PAB group and 20.14?2.48 in 10?mol/L PAB group,which was significantly lower than those in control group(46.50?4.70) and PAB 5?mol/L group(42.44?5.80,P

Objective To study the clinic characters of patients with latent autoimmune diabetes in adults (LADA). Methods We evaluated the prevalence of acute and chronic complications in the LADA patients with serum antibodies to glutamic acid decarboxylase (GADA) or to islet cell cytoplasmic antigens (ICA). The prevalence of acute and chronic complications were further compared with that in patients with acute onset type 1 or type 2 diabetes. Results The mean age, BMI, fasting and postprandial C peptide levels of the LADA patients were higher than those of type 1 diabetes and lower than those of type 2 diabetes. Of the LADA patients, 29.3% had ketosis. Although the frequency of repeated attack of ketosis in the LADA patients was similar to that in type 1 diabetes, the duration from diabetic onset to first ketosis in the LADA patients was much longer. The prevalence of retinopathy in the LADA patients was 19.5%, which was similar to that in type 1 diabetes. The prevalence of cataract in the LADA patients was 48.8%, which was similar to that in type 2 diabetes. Less LADA patients had microalbuminuria, compared with the other groups. There was no difference in the prevalence of overt nephropathy, neuropathy and hyperlipidemia among the three groups. The prevalence of coronary heart disease in the LADA patients was similar to that in type 2 diabetes. The prevalence of hypertension in the LADA patients was higher than that in type 1 but lower than that in type 2 diabetes. Conclusion The clinic characters as well as acute and chronic complications in LADA patients are different from those in type 1 and type 2 diabetes.

Objective To explore the incidence and risk factor of renal artery stenosis in patients with coronary artery disease and essentiality of renal arteriography while performing coronary artery angiography. Methods Renal arteriography was performed immediately after coronary artery angiography in 114 patients with suspected coronary artery disease. Results Incidence of renal artery stenosis was 18.4% (21/114) in 114 patients and 26% (20/77) in patients with coronary artery disease who were identified by coronary artery angiography. Only one case with renal artery stenosis was found in 37 cases whose coronary arteries were normal (2.7%, 1/37). Incidence of renal artery stenosis in patients with coronary artery disease was higher than that in patients with normal coronary artery (26% vs 2.7%, P

Objective To explore the effects of TSP in classroom air on teachershealth.Methods Air sampling was car-ried out nearby the platform in each in use-classroom at flow of100L/min continuously for6h(n=270)and in office building as controls(n=10)in a university.An investigation on the prevalence of respiratory diseases was performed also.Results The concentration s of TSP were0.10-0.88mg /m 3 in classroom air with a average level of0.38mg /m 3 ,and only0.09mg /m 3 in office air of the university,and0.194mg /m 3 in the ambient air respectively.The TSP concentrations in the classromms exceeded the National Standard of Ambient Air Quality GradeⅢ.The prevalence rates of respiratory diseases including acute and chronic phyryngitis,acute tracheobronchitis and chronic bronchitis were23.3%,73.9%,4.0%and17.4%for teachers,only4%,2.8%,3.2%and7.1%for administrative staff respectively.Con clusion The classroom air pollution by TSP had adverse effects on teachershealth in classrooms.

Objective To further analyze the chaos characteristics of heart rate variability(HRV) signals.Methods Analyzed HRV was obtained from 40 healthy subjects(20 olds,20 youngs) by means of Cao algorithm;Stability criteria was proposed to determine minimum embedding dimension;Meanwhile,we advanced a new quantative analysis method of studying HRV deterministic component,and applied the method to the old and young groups.Results Each subject's HRV was composed of both stochastic and deterministic components;Stability criteria could solve the subjective problem when determine minimum embedding dimension by Cao algorithm;Deterministic component in healthy old subjects was higher than the young.Conclusion In this paper,we have proved that HRV is deterministice chaos;Stability criteria works well for determining minimum embedding dimension;HRV decreases as age increases.

Objective:To set up a real-time fluorescent RT-PCR and to quantitate the expression levels of hTERT mRNA in peripheral blood mononuclear cells from patients with AML and to observe the correlation between the expression of hTERT mRNA and occurrence and relapse of AML and to probe into the correlation between the expression of hTERT mRNA and telomerase activity.Methods:Real time fluorescent RT-PCR and Lightcycler PCR system were used to quantitate expression levels of hTERT mRNA.PCR-ELISA was used to quantitate telomerase activity.Results:①N_(hTERT) from AML at initial presention,AML at relapse,AML at complete remission and health examinee was 299.2?292.8,550.1?441.3,14.0?9.2 and 12.3?6.7 respectively and the expression levels of hTERT mRNA from AML at initial presention and AML at relapse elevated signficantly comparing to that from AML at complete remission and health examinee,moreover the expression levels from AML at relapse was higher than that from AML at initial presention significantly.②The telomerase activity from AML at initial presention,AML at relapse,AML at complete remission and health examinee was 32.8%?24.3%,48.6%?31.4%,7.4%?5.1% and 7.6%?3.6% respectively and the telomerase activity from AML at initial presention and AML at relapse elevated signficantly comparing to that from AML at complete remission and health examinee,moreover telomerase activity from AML at relapse was higher than that from AML at initial presention significantly.③There was a strong correlation between telomerase activity and the expression of hTERT mRNA and the correlation coeffecients was 0.78.Conclusion:The up-regulation of the expression levels of hTERT mRNA and telomerase activity are one of important factors during occurrence and relapse of AML,moreover there is a strong correlation between telomerase activity and the expression of hTERT mRNA.