2.Biological characteristics and genesis of cancer stem cells
Wen QIU ; Longfei HU ; Yang LI
Journal of International Oncology 2010;37(7):492-495
Cancer stem cells is a small fraction of cells exist in cancers and own the same characteristics of stem cells. It has recently been shown that cancer stem cells in some tumours contain lower reactive oxygen species(ROS) levels and enhanced ROS defence radioresistance. The DNA damage induced by p53 tumor suppressor protein is associated with self cancer stem cells. We have reviewed the biological characteristics including self- renewal, drug resistance, heterogeneity and so on. As to the genesis of the cancer stem cells,some researchers believe that they arise from normal adult stem cells, progenitor cells or mutation of somatic cells, while others consider that cancer stem cells are derived from cell fusion and so on.
3.Preparation,Quality Control and Therapeutic Effect Observation of Chlorphenamine Cream
China Pharmacy 1991;0(04):-
OBJECTIVE:To prepare chlorphenamine cream,establish its quality control and observe its clinical therapeutic effect.METHODS:Chlorphenamine cream was prepared by emulsification,the content was determined by UV spectrophotom?etry and the stability of which was observed by the initial average rate method.RESULTS:The linear range of chlorphenamine was10~30?g/ml(r=0.9999);the average recovery rate was101.25%,RSD=1.28%;the total effective rate in the clinical application was97.66%.CONCLUSION:The cream is stable in quality and its therapeutic effect is reliable and safe.
4.Analysis on etiology and prognosis of 223 children cases of acute kidney injury
Guochang YE ; Qiu LI ; Chao WEN
Chongqing Medicine 2014;(10):1181-1183,1186
Objective To investigate the etiology and prognostic factors of acute kidney injury (AKI) in pediatric patients . Methods The clinical data of children patients with AKI in this hospital ,including the clinical features ,causes and outcomes ,were retrospectively analyzed .The relationship between the risk factors and prognosis was analyzed .Results Infants were dominated by the prerenal factors and the other age groups were dominated by the renal factors .The univariate analysis revealed that the AKI stage ,etiology ,mechanical ventilation ,sepsis/septic shock ,MODS ,acidosis ,creatinine initial value ,creatinine peak value and serum potassium were the factors affecting prognosis .The Logistic regression analysis showed that mechanical ventilation ,MODS and sep-sis/septic shock were the independent risk factors affecting prognosis .Conclusion The etiology of AKI in children is diverse and its distribution has the age characteristics .Mechanical ventilation ,MODS and sepsis/septic shock are the independent risk factors af-fecting prognosis .The early diagnosis and the active treatment conduces to improve prognosis .
6.Significance of Determining Serum Diamine Oxidase and D-Lactate in Newborn Infant with Critically Ill
qiu-wen, WEI ; lin-lin, WANG ; xiao-rong, LI
Journal of Applied Clinical Pediatrics 1992;0(06):-
0.05).Conclusions Serum DAO can be used as a sensitive marker to early diagnose gastrointestinal dysfunction in critical neonates.Serum D-lactate will be of high specific clinical significance in diagnosis,the change of D-lactate may have relations to the features of intestinal microflora in early neonates.
7.A questionnaires study on cochlear implantation in patients with white matter changes.
Wen LI ; Jianxin QIU ; Dezhi YU ; Yinping ZENG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(16):1205-1207
OBJECTIVE:
This article evaluates the auditory and speech perception outcomes of sensorneural hearing loss with cerebral white matter abnormalities after cochlear implantation.
METHOD:
A retrospective analysis was performed on the cochlear implantatees with cerebral white matter abnormalities by using the categories of auditory performance and speech intelligibility rating, and compared with the non-syndrome patients with sensorineural hearing loss. Paired t-test was used for statistical analysis.
RESULT:
There was statistical difference between normal group and white matter changes group with sensorineural hearing loss after 6 month. No statistical difference was found after 12 month and 24 month.
CONCLUSION
In the short term, the cochlear implant can be performed safely in patients with white matter changes. After a formal rehabilitation training,no significant difference in auditory or language ability was found between normal group and white matter changes group with sensorineural hearing loss.
Child, Preschool
;
Cochlear Implantation
;
rehabilitation
;
Female
;
Follow-Up Studies
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Hearing Loss, Sensorineural
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etiology
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surgery
;
Humans
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Leukoencephalopathies
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complications
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Male
8.Comparative Study on the Content of Total Flavonoid and Naringin in Different Cultivars of Citrus Grandis ‘Tomentosa’
Hongying ZHAO ; Haitao WEN ; Li LIN ; Xianxiu QIU ; Haohui LIU
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(02):-
Objective To choose a best cultivar by comparing the content of total flavonoid and naringin between different cultivars of Citrus grandis 'tomentosa'.Methods The content of total flavonoids was determined by spetral photometric analysis.The content of naringin was determined by HPLC.Results The content of total flavonoid was 19.24 % and 18.06 %,and naringin content was 11.72 % and 12.38 % in the cultivars from Dachaling and Fengwei respectively,which were much higher than the other cultivars.Conclusion The contents of total flavonoid and naringin were different in different cultivars of Citrus grandis 'tomentosa'.The cultivars of Citrus grandis 'tomentosa' from Dachaling and Fengwei have the best quality.
9.Identification and molecular mechanism study of a case with B(A)02 allele
Li QIU ; Nan YAO ; Wen MIAO ; Wei ZOU ; Xiaohong CAI
Tianjin Medical Journal 2016;44(5):625-628
Objective To identify and investigate B(A)02 allele in a patient. Methods Serological tests were performed with standard serological methods in a patient with B(A)02 allele. DNA sequences of all seven exons and exon-intron boundaries of ABO gene were analyzed by polymerase chain reaction (PCR), direct DNA sequencing and sequencing after gene cloning. In order to analyze the allele, PyMOL software was used to establish 3D model of Glycosyltransferases B (GTB). Results The serological results showed the characteristics of B(A) phenotype. DNA analysis revealed that ABO gene of the individual was heterozygous of B(A)02/O01 allele. 700C>G mutation was identified in B101 allele, which resulted in the amino acid substitution P234A in GTB. Through the analysis of the 3D structure of GTB, it was speculated that the P234A replacement affected the intermolecular forces of the 234 amino acid and Met-266, thus changed the conformation of the donor-binding pocket of GTB,that made GTB capable of recognizing and tranferring the GalNac to the H antigen, which can lead to the formation of the weak A antigen on membrane of red blood cells. Conclusion The P234A replacement can affect the spatial conformation of the specific recognition region conformed by Met-266 and Ala-268 residues, which leads to the antigenicity change of the ABO blood group.
10.Relationship between arsenic (+3 oxidation state) methyltransferase genetic polymorphisms and susceptibility to endemic arsenism
Wen-sheng, HOU ; Li-fang, ZHANG ; Qiu-ling, PEI
Chinese Journal of Endemiology 2013;(1):33-37
Objective To investigate the association between arsenic(+3 oxidation state) methyltransferase (AS3MT) genetic polymorphism and susceptibility to endemic arsenism.Methods Polymerase chain reactionrestriction fragment length polymorphism-single strand conformation polymorphism(PCR-RFLP-SSCP) technology was performed to detect mutations of AS3MT gene intron 8 and exon 9 in genome DNA of the 79 cases and 110 controls.PCR products with abnormal band forms were further sequenced to find the types and sites of mutation.Chi-square test and multivariate Logistic analyses were conducted.Results The incidence of the 9149 base mutation(A→C) in AS3MT gene intron 8(AS3MT-9149) in case group(19.0%,15/79) was lower than that in control group (23.6%,26/110).The incidence of the codon 287 mutation(ATG→AT/CG) in AS3MT gene exon 9(AS3MT-287)in case group(10.1%,8/79) was lower than that in control group (11.8%,13/110).However,statistical analysis indicated no significant difference in both mutations between two groups[AS3MT-9149:odds ratio(OR) =0.59,95% confidence interval(CI):0.26-1.31,P =0.195; AS3MT-287:OR =0.85,95% CI:0.32-230,P =0.751].Conclusions There are no significant association between the genetic polymorphisms of AS3MT-9149,AS3MT-287 and the susceptibility to endemic arsenism.Similarly,due to small sample amount,we can not exclude the possibility that these gene polymorphisms are related to susceptibility to endemic arsenism.