Following ideas are proposed for the development of library-holding resources with the need of users as its target according to the background and characteristics of big data, namely with the users-driving development of library-holding resources as its principle, with the users-satisfying resources acquisition method as its direction, and with the users-needing development of library-holding resources as its integration platform.

AlM: To investigate the risk factors of diabetic retinopathy ( DR) in type 2 diabetes patients.METHODS:A total of 380 cases of type 2 diabetes were selected from January 2014 to June 2014, divided into DR group (126 cases) and non-diabetic retinopathy ( NDR) as control group (254 cases), all patients were underwent medical history, physical examinations, laboratory and assistance examinations. The prevalence and single factor and multiple factors of DR were analysed by the logistic regression model.RESULTS: The univariate logistic regression analysis showed that DR was associated with diabetes duration, systolic blood pressure, triglyceride, total cholesterol, low - density lipoprotein, urine protein, intraocular pressure, carotid intima - media thickness, peripheral neuropathy disease was the related risk factors for DR. ln multiple logistic regression analysis, only duration was the related risk factors for DR.CONCLUSlON:The occurrence of DR is the outcome of combined action of multiple factors, duration is an independent risk factor for occurrence of DR.

【Objective】 To examine the regeneration of NPY-immuno reactivity (IR) retinal ganglion cells (RGCs) and the effects of cholera toxin ( CTx) injected or/and peripheral nerve implanted on regeneration of NPY-IR RGCs. 【Methods】 16 adult golden hamsters were ramdomly divided into 4 groups. Optic nerve (ON) was transacted and a segment of autologous sciatic nerve (attached g raft, AG) was removed and sutured to the proximal stump of ON in regenerating co ntrol group (AG group). The animals in experimental groups were further treated with CTx injection or/and implantation of a short of segment sciatic nerve (SN) intravitrously. By using the retrograde labeling with fluorogold (FG) combined w ith fluorescent immunocytochemistry, the regenerated NPY-IR RGCs were observed and counted under fluorescent microscope. 【Results】 At 4 weeks after surgery, the mean number of regenerated NPY-IR RGCs per retina in AG group was 58±22 wh ich constitutes (3.36±0.65)% of the total regenerated RGCs. In AG+CTx, AG+SN and AG+CTx+SN experimental groups, the mean numbers of regenerated NPY-IR RGCs per retina were 143±47, 125±37 and 437±77 ordinally which constitute (5.15± 0.89)%, (5.34±0.37)% and (8.11±0.85)% of the total regenerated RGCs respec tively, which were increased significantly compared with those in AG group. 【Co nclusion】 The results show that the axotomized NPY-IR RGCs have th e capability of regenerating their axons into the attached PN graft, CTx and/or SN could enhance the regeneration of the NPY-IR RGCs.

Objective The present study was aimed at investigating the effects of cholera toxin(CTx) on promoting the regeneration of retinal ganglion cells(RGCs) in hamster retina. Methods After optic nerve (ON) transection, an autologus sciatic nerve (attached graft, AG) was removed and sutured to the proximal stump of the ON. CT X was injected or (and) a small segment of sciatic nerve (SN) inserted intravitrously. Animals were separated into 5 groups:regenerating control group(AG groups and solution groups); AG+CT X groups; AG+SN groups; AG+CT X+SN groups; Effect and Dosage groups; Animals in the former 4 groups were allowed to survive for 2-6 weeks respectively. The regenerated RGCs were labeled retrogradely by granular blue, and the changes in number of regenerating RGCs in each retina were observed under fluorescent microscope. Results The mean numbers of regenerating RGCs in AG+CT X groups were increased and significantly higher than those in AG groups and solution groups at each time point( P

Detection of high-risk human papillomavirus ( HPV) gene is essential in cervical cancer screening.This paper presents some HPV detection methods according to different molecular techniques , mainly introducing the commonly used hybird capture Ⅱ, HybriMax and method based on fluorescent quantitative PCR , elaborates their advantages and shortcomings , and discusses the clinical applications in the future.

AIM: To explore the main risk factors related to the incidence of endophthalmitis in patients after cataract surgery in China and to provide evidence for prevention.METHODS: The results of 5 studies on the main risk factors of endophthalmitis in patients after cataract surgery were analyzed by Meta-analysis method.
RESULTS: The pooled odds ratio values and 95% CI of age(≥70), diabetes, vitreous overflow, operative time ( ≥ 10min ), common operating room and control of using time of topical anesthetic were 1. 81(95% CI: 1. 43-1. 69),3. 66 (95% CI: 1. 64 - 8. 16),2. 21 (95% CI: 1. 46 -3. 32),3. 54 (95% CI: 2. 47 - 5. 06),2. 77 (95% CI: 2. 07 -3. 72),2. 09(95% CI: 1. 53-2. 86).
CONCLUSION: The main risk factors of endophthalmitis were the age(≥70), diabetes, vitreous overflow, operative time(≥10min), common operating room and control of using time of topical anesthetic.

OBJECTIVE:To establish a HPLC method for the determination of cilostazol in human plasma.METHODS:C18 was the chromatographic column,MeOH-0.03mol/L ammonium acetate (62∶38,V/V)was the mobile phase with a flow rate at 0.8 ml/min and detection wavelength at 257 nm,acetic ether was used as the extraction solvent.RESULTS:Good linear relationship was achieved when the detection concentration range of cilostazol was 10.0～1 500.0 ng/ml(r=0.9 999),the average recovery rates for the high,medium and low concentrations of cilostazol were 99.82%,97.30%and 97.14%,respectively,both the intra-day and inter-day RSD were lower than 6%.CONCLUSION:This method is sensitive,accurate,simple and fast,which can be used as the clinical monitoring of blood concentration of cilostazol and its pharmacokinetics study.

Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Female ; Humans ; Lung Neoplasms ; drug therapy ; pathology ; radiotherapy ; Male ; Middle Aged ; Phytotherapy ; Radiation Injuries ; prevention & control ; Spinal Neoplasms ; drug therapy ; secondary ; Treatment Outcome

OBJECTIVE To observe the clinical therapeutic effect of levofloxacin combined with cefoperazone-sulbactam on pulmonary infection caused by multidrug-resistant(MDR) bacillus infection.METHODS Eighty five patients with pulmonary infection caused by MDR bacillus infection were randomly divided into treatment group and control group,combined levofloxacin and cefoperazone-sulbactam were used in 45 patients of treatment group,ceftriaxone sodium was used in 40 patients of control group,their therapeutic effect was observed 7 days later.RESULTS Thirty four patients of treatment group were cured,7 patients with excellence,3 patients with improvement and only 1 patient was invalid.The total effective rate was 91%.Clinical manifestation such as cough,expectoration,and dyspnea had obviously improved.Sputum culture darkening rate was high.CONCLUSIONS Combined therapy of levofloxacin and cefoperazone-sulbactam has definite therapeutic effect on pulmonary infection caused by MDR bacillus infection.

Objective To investigate the demands of PACS system on the storage and the ways to optimize its performance.Methods The storage media,such as CD Rom library and disc array,were integrated,and three-tier network structure was established.Results Online storage through virtual storage technology could be applied to high-capacity,high-quality and high-reliability medical image storage.Conclusion Storage optimization PACS is implemented.