Objective Based on the result that a gene coding for human HSS had been identified and prokaryotically expressed, this study is aiming to investigate the proliferative and protective effect of recombinant HSS on hepatoma cells. Methods hHSS protein was produced in BL\|21 strain of E.Coli containing pET\|42a vector and purified with His\5Tag affinity chromatography. A various dosages of 80\|400??g/L were administrated into cell culture. The cell proliferation was analyzed by MTT, cell\|count and flow cytometry methods. Furthermore, cellular growth signaling as indexed by phosphorylation of mitogen\|activated protein kinase (MAPK) was determined with Western blot. In addition to cell growth, protection of hHSS on the cells against H\-2O\-2 was also observed. Results It was showed that recombinaht HSS of 400??g/L was able to promote DNA synthesis by 21\^5% as compared to non\|treated cells. After 24?h of HSS action, the cell division measured by MTT method as well as cell\|count was significantly enhanced. Meantime, it was indicated that the phosphorylation of MAPK Thr202/Tyr204 was increased in 79\^0% as compared with that of the non\|treated cells. Pretreatment of the cells with hHSS for 12?h prior to H 2O 2 injury preserved the survival of them.Conclusion It is postulated that hHSS is an active protein to stimulate liver proliferation. [

G protein- coupled receptors (GPCRs), also known as seven- transmembrane domain receptors, constitute the largest superfamily of cell surface receptors. By coupling to heterotrimeric G proteins, arrestins and other signaling molecules, GPCRs modulate diverse signal transduction pathways under physiological and pathological conditions. Recent studies have revealed crucial roles of GPCRs in tumorigenesis and development of cancer metastasis. This review summarizes roles of GPCRs, particularly the roles of those coupled to chemokines, prostaglandin, lysophosphatidic acid, endothelin, catecholamine and angiotensin in proliferation, invasion, metastasis and angiogenesis of hepatoma cells and development of hepatocellular carcinoma. The potential of GPCRs- based therapeutics being used for hepatocellular carcinoma is also highlighted.

This list of clincal data management documentation is to ensure standardized and adequate archival of trial documents and records in clinical data management, which is applicable to all of phase I-IV clinical trials.

Adult ; Delirium ; chemically induced ; Female ; Humans ; Thiocarbamates ; poisoning

Objective To explore the curative effect of coronary artery bypass graft (CABG) in the treatment of acute coronary syndrome (ACS).Methods Clinical data of 64 patients with ACS were retrospectively analyzed,41 patients received CABG (CABG group) and 23 patients received percutaneous coronary intervention (PCI,PCI group).Mainly cardiac events and secondary cardiac events were compared.Results The incidence rate of cadiovascular and cerebrovascular events during hospitalization in CABG group was 7.32％(3/41),in PCI group was 8.70％ (2/23),there was no significant difference (P ＞ 0.05).All patients accomplished follow-up and follow-up time was 12-48 months.The meta follow-up time in CABG group was 35.26 months,in PCI group was 33.19 months.The incidence rate of cadiovascular and cerebrovascular events during follow-up in CABG group was 19.51％ (8/41),in PCI group was 30.43％(7/23),there was no significant difference (P＞ 0.05).The incidence rate of death,myocardial infarction,cerebrovascular events,angina pectoris and total cadiovascular and cerebrovascular events between two groups had no significant difference(P＞ 0.05).Conclusions The surgery treatment in ACS is feasible,the curative effect of CABG and PCI is therapeutic equivalence.The choice of operation should be based on patients' condition and individual choice.Appropriate revascularization can get the best treatment efficiency.

[Objective] Study effect of Yun-Pi Prescription on regulating the immune function,approach the mechanism of Yun-Pi Prescription on how to enhance immune function.[Method]All the mice were divided into groups: the normal group,the model group,the positive control group treated with Xiao Shi Jian Er Syrup,and Yun-Pi Prescription treatment groups including high,middle and low dose;determine the index number of immune organ,phagocytic count and phagocytize activity of normal mice.[Results]The high and middle dose of Yun-Pi Prescription can obviously enhance the phagocytic count and phagocytize activity,Yun-Pi Prescription has an up-regulation effect on immune function.

[Objective]To explore the biomechanical stability of fixation with two and three cannulated compression screws in treatment of the adductive femoral neck fractures by the three-dimensional finite element analysis(FEA).[Method]Two FEA models of two screws and three screws for adductive femoral neck facture fixations were set up to investigate the simulated loading of three different conditions.[Result]At the condition of the heel touching the ground and the middle position,there was no difference in the biomechanical stabilities of the two models.However,at the condition of the tiptoe departing from the groud,the model of three screws fixation was better in torsion resistance and stretching resistance.[Conclusion]The biomechanical stability of fixation with two screws is determined by the direction of loading.Three cannulated compression screws fixation provides better biomechanical stability.

0.05).Their a verage values were 6.47?0.80,4.36?0.68,9.89?0.73,5.83?1.63, 3.55 ?0.69 an d 6.81?0.93 respectively. In the male and famale LRa-TC was 3.84?1.16 and 3.1 5?0.76(P0.05).Their average v alues were 5.44?1.03,4.09?1.58,3.75?0.75,4.20?0.84 and 7.70?1.13 respective ly. In the male and famale URa-PC was 7.14?1.16 and 6.62?0.88(P0 .05).Their average values were 6.06?0.91,3.20?1.48,4.52?0.69 and 8.53?0.98 r espectively. In the male and femal URa-PC was 7.49?1.01 and 6.94?0.74(P

Objective To study the protective mechanism of Gross Saponin Tribulus Terrestris(GSTT) on the neonatal rat ventricular cardiocytes injured by hypoxia and the effect on protein kinase C(PKC).Methods The hypoxia-ischemia model was performed by treating the cultured neonatal rat ventricular cardiocytes with NaCN.The effect of GSTT(100 and 30 mg?L~(-1)) on the contents of ?PKC and ?PKC were detected by flow cytometry and laser confocal microscopy system.Results GSTT up-regulated ?PKC and ?PKC expressions.The contents of ?PKC and ?PKC in GSTT 100 mg?L~(-1) group(1 325.00?53.25,810.55?36.89;66.22?6.23,40.12?2.21) were increased significantly than those in model group(792.00?32.36,492.40?30.15;32.70?2.78,29.28?4.82)(P

Objective To evaluate the reliability of a series of non-invasive methods in the presurgical lateralization of mesial temporal lobe epilepsy(MTLE).Methods The results of the interictal scalp electroencephalogram(EEG)and ictal scalp EEG,clinical seizure symptom,MRI and interictal SPECT obtained from 40 patients with MTLE who had been followed up 1 to 4 years without seizure after anterior temporal lobectomy(ATL)retrospectively were analyzed in Xuanwu Hospital from May 2002 to May 2005.Results (1)Unilateral anterior temporal spikes were found on interictal EEG in 37(92.5%)patients,of whom 35(94.6%)were in accordance with the lateralization of epileptogenic focus.(2)Ictal scalp EEGs were recorded in 32 patients,from which epileptogenic foci were lateralized in 26 of the 32 patients(81.2%),the concordant rate being 96.2%.(3)Twenty-three patients(57.5%)had clinical seizure symptoms referring to lateralization,of whom 19(82.6%)had symptoms identical to the lateralization of epileptogenic focus.(4)Thirty-eight patients(95.O%)showed structural abnormalities of unilateral hippocampus or temporal lobes on MRI which were in accordance with the lateralization of epileptogenic focus in 37 patients.(5)Interictal SPECT was measured in 23 patients,which was identical with the lateralization in 18/22(81.8%).Conclusions Among a series of non-invasive methods in the presurgical lateralization of mesial temporal lobe epilepsy,SPECT is the most sensitive one,then sequenfly comes MRI,ictal scalp EEG,interictal scalp EEG and clinical seizure symptom.MRI is the most reliable one,then comes ictal scalp EEG,interictal scalp EEG,clinical seizure symptom and SPECT in a sequent order.