1.Research of oxaliplatin inhibit growth in glioma U87 cells by regulating PI3K/Akt signal pathway
Youqiang DUAN ; Yifeng LIU ; Wei LI
Chinese Journal of Immunology 2017;33(3):328-332
Objective:To investigate the effect of oxaliplatin inhibit the growth in glioma U 87 cells by regulating PI3K/Akt sig-nal pathway.Methods:The glioma U87 cells were cultivated in vitro ,using 0,20,40,80 μg/ml oxaliplatin treated U87 cells 24,36, 48,72 h respectively,MTT was used to detect cell proliferation.Using 40 μg/ml oxaliplatin treated U87 cells 48 h,flow cytometry was used to detect cell cycle and apoptosis .Cells apoptosis protein and PI 3K/Akt pathway protein expression after 40 μg/ml oxaliplatin treated U87 cells 48 h was detected by Western blot .Results:MTT assay showed that compared with the 0μg/ml treatment ,oxaliplatin treatment could significantly inhibited U 87 cell survival ( P<0.01 ) ,40μg/ml oxaliplatin treatment 48 h ,the survival inhibitory was the most obvious.U87 cell cycle was arrested in S phase after 40 μg/ml oxaliplatin treatment 48 h.After 40μg/ml oxaliplatin treatment 48 h,compared with the 0 μg/ml treatment,U87 cell apoptosis rate significantly increased (P<0.01).Western blot showed that after 40μg/ml oxaliplatin treatment , anti-apoptotic factor Bcl-2 expression had significant decreased , pro-apoptotic factors Bax , Cleaved-caspase3 protein expression had significantly increased (P<0.01).PI3K,p-Akt expression was significantly decreased (P<0.01),and Akt expression had no significant change in Akt pathway (P>0.05).Conclusion:Oxaliplatin may suppress U87 cell proliferation,ar-rest cell cycle,and promote apoptosis by inhibit PI3K/Akt signaling pathway.
2.Effects of Microfiltration Membrane Adsorption on Detection of Pesticides in Water by Direct Injection Liquid Chromatography-Tandem Mass Spectrometry
Wei LI ; Yucan LIU ; Jinming DUAN
Chinese Journal of Analytical Chemistry 2015;(11):1761-1765
Effects of microfiltration membrane adsorption for eight types of pesticides with diverse physicochemical properties on direct injection liquid chromatography-tandem mass spectrometric method ( DI-LC-MS/MS) were investigated. The results showed that the adsorption effects ( AE,%) on the loss of signal detection at concentration of 2. 5 μg/L ranged from 8%-92%, 0%-81% and 2%-59% for nylon (NYL), polyethersulfone ( PES) and hydrophilic polypropylene ( GHP) membrane, respectively ( accumulated volume of feed is 1 mL). The pesticides with higher octanol-water partition coefficient (lgKow) values appeared to adsorb onto the membrane surface more readily than those with lower lgKow. The AE was found to be alleviated as the feed volume of samples increased to 10 mL, except the case of profenofos adsorption on PES membrane ( AE=9%) . The effects of PES membrane on the eight pesticides at initial individual concentration of 0 . 25 , 2. 5 and 25 μg/L were also studied. The results showed that, the lower the initial concentration of pesticides, the stronger adsorption effect under the same filtration conditions was obtained. Besides, it was effective in eliminating the membrane adsorption effects by adding 40% methanol ( V/V) into water samples as a modifier, which could consequently improve the accuracy of DI-LC-MS/MS.
3.Effects of propofol pretreatment on S100? and neurosecretion enzyme in rat brain tissues with global cerebral ischemia-reperfusion
Guosheng GAN ; Wei DUAN ; Li JIANG
Medical Journal of Chinese People's Liberation Army 2001;0(11):-
Objective To study the effects of propofol pretreatment on S100? and neurosecretion enzyme (NSE) of brain tissues with global cerebral ischemia-reperfusion in rat, and to evaluate the effects of propofol in protection of brain. Methods 30 SD male rats were randomly divided into 3 groups: sham operation group (group A, n=10); single cerebral ischemia-reperfusion group (group B, n=10); propofol pretreatment at 2h before ischemia group (group C, n=10), in which propofol (100mg/kg) was given intraperitoneally (ip) before ischemia. 24h after ischemia-reperfusion, the neuroethology scores were recorded and evaluated, and S100? and NSE in rat brain were determined. Results The neuroethology scores of group A were higher than those of group B (P
4.Analysis of dual energy CT imaging in the diagnosis of gouty tophus deposition value
Yanfeng DUAN ; Wei LI ; Wei YANG ; Longyu GUO ; Juan WANG
Chongqing Medicine 2017;46(4):491-493
Objective To explore the value of dual energy CT imaging in the diagnosis of gouty tophus deposition.Methods Sixty-five cases of patients with gout treated in this hospital between December 2012 to December 2015 were selected.Another 65 cases of patients(rheumatoid arthritis,degenerative osteoarthritis and unexplained leg pain) were selected as control group.The re sults of dual energy CT imaging between two group were compared.Three experienced clinicians who didn't understand the imaging results were selected.They had a medical history and physical examination in patients with gout.The differences between clinical evaluation and imaging results were compared.Results Dual energy CT imaging showed that there were no tophi deposition in con trol group;the topi deposition in wrist joint,knee joint and ankle joint were 6.06%oo (33/34),100.00% (22/22) and 96.49% (55/57) in gout group.Dual energy CT imaging detected a total of 545 parts of tophus deposition.It was 4.36 times of the result of clinical evaluation(125 parts).Conclusion Dual energy CT imaging shows the advantages of simple noninvasive,high accuracy and low rate of misdiagnosis in diagnosing gout nodules.
5.Nardosinone reduces neuronal injury induced by oxygen-glucose deprivation in primary cortical cultures.
Wei LI ; Jinli SHI ; Qin LI ; Huihui DUAN ; Minke TANG
Acta Pharmaceutica Sinica 2013;48(9):1422-9
The aim of the study is to investigate the effect of nardosinone (Nar) on neuronal injury induced by oxygen-glucose deprivation (OGD) in primary cortical cultures isolated from embryos at gestational day 14. MTT method was used to determine the dosage regimen of Nar in primary neuronal cultures and observe the influence of Nar on the neurons suffering OGD; Western blotting analysis was used to detect expressions of protein kinase A (PKA), Ras related protein 1 (Rap1), mitogen-activated protein kinase kinase 1 (MEK1) and phospho-extracellular signal-regulated kinase 1/2 (p-ERK1/2) of OGD-injured or uninjured primary cultured neurons after Nar treatment. Results showed that Nar (50 and 100 micromol x L(-1)) improved the cell viability during OGD damage (P < 0.01) and increased the expression of PKA, Rap1, MEK1 and p-ERK1/2 in injured neurons. Additionally, elevations of PKA, Rapl, MEK1 and p-ERK1/2 in uninjured neurons were caused by Nar (50, 100 and 200 micromol x L(-1)) with a dose-dependent tenclency as well (P < 0.01). In conclusion, Nar could protect against the neuronal injury exposed to OGD, which may be relevant to the promotion of PKA and ERK signaling pathway.
6.Value of Multi-slice CT in Diagnosis of Papillary Renal Cell Carcinoma
Tianjiao JIANG ; Li ZHENG ; Chongfeng DUAN ; Wei LI ; Qing YANG
Chinese Journal of Medical Imaging 2013;(11):857-860,863
Purpose To evaluate the value of multi-slice spiral computed tomography (MSCT) scan in the diagnosis of papillary renal cell carcinoma. Materials and Methods A retrospective study was conducted on the findings of plain CT scan and triphasic dynamic contrast enhanced CT scan of 23 patients with papillary renal cell confirmed pathologically, and further contrasted with the findings of 96 patients with clear cell renal cell carcinoma at corresponding phases in such aspects as cystic degeneration, enhancement degree and pattern (homogeneous, peripheral or heterogeneous) and tumor spreading pattern (perinephric invasion, lymphadenopathy or venous invasion). Results The degree of enhancement CT value for both diseases showed statistical significance in corticomedullary phase, parenchymal phase and excretory phase (t=9.70, 8.08, 5.92;P<0.01), but papillary renal cell carcinoma was lower than clear cell renal cell carcinoma. In terms of cystic degeneration, papillary renal cell carcinoma was significantly lower than clear cell renal cell carcinoma (21.7%vs 54.2%,χ2=7.54, P<0.01). Papillary renal cell carcinoma mainly showed homogeneous enhancement (60.9%) whereas clear cell renal cell carcinoma mainly presented heterogeneous enhancement (68.8%) with statistical difference (χ2=30.40, P<0.01). The tumor spreading patterns like perinephric invasion, lymphadenopathy and venous invasion had no significant difference (χ2=0.51, 0.11, 0.03;P>0.05). Conclusion Papillary renal cell carcinoma shows homogeneous enhancement, seldom cystic degeneration or necrosis on MSCT scan, which is helpful for the differential diagnosis from clear cell renal cell carcinoma.
7.Wuling Capsule promotes hippocampal neurogenesis by improving expression of connexin 43 in rats exposed to chronic unpredictable mild stress.
Deqiang LI ; Xujuan LI ; Jinfeng DUAN ; Wei CAI
Journal of Integrative Medicine 2010;8(7):662-9
Objective: To investigate the effects of Wuling Capsule, a compound traditional Chinese herbal medicine, on hippocampal neurogenesis by examining the expressions of brain-derived neurotrophic factor (BDNF) and connexin 43 (Cx43) in rats with depression induced by chronic unpredictable mild stress (CMS), and thereby to explore its antidepressant mechanism. Methods: Forty-five adult male Sprague-Dawley rats were randomly divided into three groups: control group (n=15), untreated group (n=15) and Wuling group (n=15). All rats except those in the control group were subjected to 3-week CMS to induce depression. At the same time Wuling Capsule was daily added to the diet of the rats in the Wuling group at a dose of 100 mg/kg body weight for 21 days. The degree of depression was determined by sucrose preference test. BDNF expression and neurogenesis were tested by using immunohistochemical staining with BDNF and 5-bromodeoxyuridine (BrdU) antibodies; and the mRNA and protein expression levels of Cx43 in hippocampus were examined by semi-quantitative reverse transcription-polymerase chain reaction and Western blotting. Results: The numbers of BDNF-positive neurons and BrdU-positive particles in dentate gyrus (DG) were significantly decreased in CMS rats as compared with the normal rats, and the same changes were found in Cx43 mRNA and protein expressions. After Wuling Capsule treatment, the depressed behaviors were improved. Moreover, the reduced expression levels of Cx43 mRNA and protein and fewer newborn neurons induced by CMS were recovered to the normal levels. However, BDNF-positive cells remained low in DG. Conclusion: Wuling Capsule can improve the low hippocampal neurogenesis in rats subjected to CMS and the antidepressant effects are related to enhancing the Cx43 expression but not through BDNF mediation.
8.Discussion on Accessible Bathroom Design for Persons with Physical Disability: A Case Study of Manual Wheelchair User
Gaofeng LI ; Jinjuan DUAN ; Qing LAI ; Chengjing WEI
Chinese Journal of Rehabilitation Theory and Practice 2013;19(4):393-397
This paper elaborated the significance of accessible bathroom design, and analyzed the physiological, mental and behavioral characteristics of persons with physical disability. According to their special needs and accessible design concepts, the design principles which consist of safety, independence, individuality, usability and amenity were discussed. Besides, the design points for building modules and facilities were summarized.
9.Diagnostic approaches of cervical glandular intraepithelial neoplasia
Shuli YANG ; Weimin KONG ; Yumei WU ; Wei DUAN ; Li ZHU
Chinese Journal of Obstetrics and Gynecology 2013;(5):344-347
Objective To investigate diagnostic approaches of cervical glandular intraepithelial neoplasia (CGIN) for improving the diagnostic levels of CGIN.Methods Clinical data of 106 cases with CGIN admitted in hospital from Jan.2008 to Dec.2010 were analyzed retrospectively.All data from preoperative thin-prep cytologic test (TCT),cervical biopsies and postoperative pathological examination of the excised cervical tissues were reviewed.Results Among 106 patients,62 cases (58.5%,62/106) were low grade CGIN (L-CGIN),44 cases (41.5%,44/106) were high grade CGIN (H-CGIN) ; 25 cases (23.6%,25/106) were pure CGIN and 81 cases (76.4%,81/106) were CGIN mixed with cervical intraepithelial neoplasia (CIN).Fifteen cases (14.2%,15/106) were found atypical glandular cell (AGC)by TCT..In the 15 cases,there were 4 cases (6.5%,4/62) L-CGIN,and 11 cases (25.0%,11/44)H-CGIN,there was significant difference between the two groups (P < 0.05) ; among 15 cases with AGC,11 cases of them (44.0%,11/25) were pure CGIN,4 cases (4.9%,4/81) mixed with CIN,in which there were significant difference (P <0.01).Seven cases (25.0%,7/28) were detected glandular lesions in 28 cases by endocervical curettage (ECC).Totally 23 cases (22.8%,23/101) were detected CGIN by colposcopy-directed biopsy,11 cases (19.0%,11/58) were with L-CGIN,12 cases (27.9%,12/43)H-CGIN,there was no significant difference between them (P > 0.05).Among the 23 cases,13 eases(52.0%,13/25) were pure CGIN,10 cases (12.3%,10/81) CGIN mixed with CIN,which showed significant difference (P < 0.01).All 106 patients were treated,101 cases treated with cervical conization and 5 cases performed hysterectomy; 23 cases were diagnosed CGIN preoperation,the ratio of preoperative diagnosis was 21.7% (23/106),83 cases (80.3%,83/106) diagnosed postoperatively.Conclusions Routine diagnostic methods of CGIN were not satisfaction,most CGIN were diagnosed after cervical resection.Cervical conization may play a very important role in diagnosis of CGIN.The positivity of TCT in H-CGIN was higher than L-CGIN.There was no different in diagnosing different CGIN grades by colposcopy-directed biopsy.The ratio of preoperative diagnosis of pure CGIN was higher than those with CGIN mixed with CIN.
10.Clinical analysis of endoscopic treatment of recurrent idiopathic pancreatitis
Hong CHANG ; Yonghui HUANG ; Liping DUAN ; Wei YAO ; Ke LI
Chinese Journal of Pancreatology 2012;12(2):83-85
Objective To investigate the characteristics of etiology,efficacy of endoscopic management for recurrent idiopathic pancreatitis (RIP).MethodsThe clinical data of 58 cases of RIP diagnosed in our hospital from April 2005 to April 2011 were retrospectively analyzed.All the patients underwent endoscopic retrograde cholangiopancreatography (ERCP),and patients with suspected sphincter of Oddi dysfunction received manometry.According to the clinical and ERCP manifestations,the etiologies of RIP were determined and individualized endoscopic treatment was applied.The patients were followed-up postoperatively about the improvement of abdominal pain and recurrence of RIP.ResuItsFifty-eight patients (29 males,29 females) were suffered from acute pancreatitis from 3 to more than 10 times.The etiologies were as follows:29 cases of biliary microlithiasis,19 case of sphincter of Oddi dysfunction ( 16 cases of pancreatic type,3 cases of mixed type),4 cases of anomalous arrangement of the pancreaticobiliary duct,and 6 cases of normal manifestations at ERCP.Biliary sphincterotomy alone was performed in 33 patients,while both biliary and pancreatic sphincterotomy was performed in 8 patients,and pancreatic sphincterotomy alone was performed in 17 patients,after sphincterotomy,pancreatic stent insertion was performed in 24 patients.The follow-up data was obtained from 41 out of 58 patients,the follow-up period ranged from 3 ~ 67 months ( average 33 months).During this period,9(22.0% ) patients suffered from RIP,and the treatment efficiency was 78%.ConclusionsBiliary microlithiasis and sphincter of Oddi dysfunction are the main causes of RIP.Drink could induce RIP.ERCP has definite treatment efficacy for RIP.