Objective To investigate the expression of vascular endothial growth faetor(VEGF) proliferating cell nudear antigen(PCNA) and their biological significance was studied in papillary thyroid carcinoma and normal thyroid tissues. Methods Immuohistochemismy S-P technique was used to examine the expression of VEGF and PCNA in specimens of 58 papillary thyroid carcinoma and 19 normal thyroid tissues. Results The positive rates of VEGF and PCNA in papillary thyroid were higher than those in normal thyroid tissues( P＜0.01 ), and a positive correlation between these two expressions existed in papillary thyroid carcinoma. Conclusion VEGF and PCNA could be regarded as parameters to evaluate the biological behavior and they might be the indices for juvant diagnosis and evaluation of papillary thyroid cancer.

AIM: To compare the visual effects of optimized aspheric transition zone Laser in situ keratomileusis (OAT z-LASIK )versus conventional Laser in situ keratomileusis (con. LASIK).METHODS: In a prospective study 44 patients (88 eyes)underwent OAT z -LASIK and 50 patients (92 eyes) received conventional LASIK surgery. Visual acuity,refractions、contrast sensitivity (CS),glare sensitivity (GS), and increment of Q-value after surgery were studied.RESULTS: All of operations on 94 cases were successful without severe complication after 3 months' follow-up. There was no significant difference in term of postoperative refractions between the two groups (t-test,P＞0.05). The uncorrected visual acuity (UCVA), CS, GS, and increment of Q-value of OAT z group were significantly better compared to the tradition group 3 months after the surgery (P＜0.05).CONCLUSION: OAT z - LASIK is a more effective procedure to improve visual quality compared to conventional LASIK.

Objective To investigate the expression of hypoxia-inducible factor 1α (HIF-1α)gene in rat cerebral cortex neurons under hypoxia conditions,and provide more experimental basis for clinical treatment of hypoxic brain disease.Methods The rat cerebral cortical neurons in primary culture and hypoxia model were prepared and identified by immunocytochemistry analysis.The expression of HIF-1α in normal and hypoxic neurons was detected at 4 time points (12 h,24 h,48 h and 72 h) by immunohistochemical analysis.Results The expression of HIF-1α positive cells in normal control (NC) group was less at each time point,and it showed no statistically significant within groups.The weak expression of HIF-1α was found at 12 h in hypoxic group,and the expression augmented along with the time extended,it increased obviously at 24 h,reached the peak at 48 h(IOD＝0.27±0.02,F＝35.703,t＝11.795,P＜0.01),and declined until 72 h,it showed statistical significance between two groups at each time point.Conclusions The expression of HIF-1α is increased after hypoxia.HIF-1α has a protective effect on neurons after hypoxia.

Objective To understand Toxoplasma gondii infection and awareness condition of married childbearing women in Chengde Region so as to provide the evidence for the establishment of control measures. Methods Totally 733 married childbearing women who took physical examination in Chengde Hospital of Traditional Chinese Medicine from July to December in 2013 were investigated by questionnaire to understand the awareness condition on T. gondii infection then 490 women among them from 3 counties and 2 districts were randomly chosen to detect the Toxoplasma antibodies by ELISA. Results A total of 733 questionnaires were returned and 126 women knew related knowledge about T. gondii infection and the awareness rate was 17.19% 126/733 . Sixty-three women were determined as infected cases and the infection rate was 12.86% 63/490 . The infection rates of the women who with higher educational level working as medical staff and living in urban were lower and the awareness rates of them were higher. Conclusions The infection rate of T. gondii among the married childbearing women in Chengde Region is high and the awareness rate of them is low. In order to decrease the infection rate as well as to increase the awareness rate of the population the health education should be strengthened.

Infantile diarrhea is pediatric clinical common diseases. In this paper, according to the related literature in recent years, the effect mechanism of massage treatment of infantile diarrhea, according to syndrome differentiation treatment based on different massage for parting with Chinese medicine and massage, acupuncture and so on has carried on the induction.

The aim of this study was to determine the role of neutrophil collagenase in the pathogenesis of acute lung injury induced by endotoxin. 28 Sprague-Dawley were randomized into control group and LPS-enduced groups. Samples of left lung were obtained in 2 h (group L1), 6 h (group L2), 12 h (group L3) after intravenous LPS. Immunohistochemsitry was employed for detection of expression of neutrophil collagenase. Pathological scores, lung wet/dry weight ratio and the number of neutrophils were measured. The results showed that the concentration of neutrophil collagenase in LPS-enduced groups (group L1, L2, L3) were significantly higher than that of control group (P<0.01). Pathological scores, lung wet/dry weight ratio and the number of neutrophils in LPS-enduced groups (group L1, L2, L3) were also significantly higher than that of control group (P<0.01). Moreover, among group L1, L2 and L3, there were significant correlations in concentration of neutrophil collagenase and pathological scores, lung wet/dry weight ratio, the number of neutrophils (P<0.05). The present study showed that neutrophil collagenase play an important role in the pathogenesis and progress of endotoxic acute lung injury.
Endotoxins ; Lung/pathology ; Matrix Metalloproteinase 8/*metabolism ; Random Allocation ; Rats, Sprague-Dawley ; Respiratory Distress Syndrome, Adult/chemically

BACKGROUND: In recent years, foreign scholars have found that apolipoprotein E gene was associated with osteoporosis and its complicated hip fracture. National scholars adopted polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to primarily study the correlation of apolipoprotein E gene polymorphism with bone mineral density (BMD) and osteoporosis.OBJECTIVE: To observe the polymorphism of apolipoprotein E gene, and analyze its correlation with primary osteoporosis and osteoporotic fracture.SETTING: Department of Orthopaedics, Nanchang First Hospital.DESIGN: A clinical controlled observation.PARTICIPANTS: Sixty patients with primary osteoporosis (31 males and 29 females), were enrolled, including 30 cases of hip fracture, who were newly fractured and diagnosed according to radiogram, and 30 cases of simple osteoporosis; Thirty healthy subjects (16 males and 14 females)were also involved.METHODS: Sixty outpatients and inpatients with primary osteoporosis were selected from the Department of Orthopaedics, Nanchang First Hospital from January to December 2002, and they were divided into hip fracture group (n=30) and simple osteoporosis (n=30); Another 30 healthy physical examinees were taken as the healthy control group. All the subjects were fasted for more than 12 hours, and the samples of venous blood were collected on the next morning. DNA was extracted from the separated leucocytes. Gene site DNA amplification was analyzed with polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). The individual DNA templates (provided by Daan Gene Diagnosis Center of Sun Yat-sen University) of identified apolipoprotein E2/2, E3/3 and E4/4 homozygotes were analyzed with PCR-SSCP, and the band showed that E3 was in the front, E2 in the middle, followed by E4. They were in electrophoresis with DNA templates of the samples at the same time, and the apolipoprotein E genotype of the template could be identified by the migration rate of the band.MAIN OUTCOME MEASURES: Apolipoprotein E genotypes were observed.RESULTS: All the enrolled subjects were involved in the analysis of results. ① Six genotypes were detected with PCR-SSCP in the 60 patients with primary osteoporosis and 30 healthy controls, including apolipoprotein E2/2, E3/2, 3/3, E4/2, 4/3, 4/4. ② There were very significant differences in the allele frequency of apolipoprotein E4 among the three groups (χ2=17.520, P ＜ 0.01). The allele frequencies of apolipoprotein E4 in the simple osteoporosis group and osteoporotic fracture group were obviously higher that that in the normal control group (χ2=4.904, 16.681, P ＜ 0.01),it was also markedly higher in the osteoporotic fracture group than in the simple osteoporosis group (χ2=4.658, P ＜ 0.01).CONCLUSION: Apolipoprotein E4 is closely associated with primary osteoporosis and osteoporotic fracture. Apolipoprotein E is a useful marker for primary osteoporosis, especially for osteoporotic fracture.

Objective In the LPS-induced acute lung injury (ALI) there is massive accumulation of neutrophils in the lung. The effect of neutrophil collagenase on the degradation of lung matrix collagen ig still unknown. The aim of this study was to investigate the role of neutrophil collagenase in LPS-induced ALI.Methods Thirty SD rats of either sex weighing 190 ? 40 g were randomly divided into five groups of 6 animals : group 1 control and group 2-5 LPS. In LPS groups animals were anesthetized with intraperitoneal 3% pentobarbital 30 mg?kg-1. Right jugular vein was cannulated. LPS (O55: B5 Sigma USA) 5 mg?kg-1 was injected iv. The animals were sacrificed at 2 h (group 2), 4 h (group 3), 6 h( group 4) and 8 h( group 5) after iv LPS administration. Bronchoalveolar lavage was performed immediately with 3 ml of normal saline. The fluid washed out was collected and subjected to hypothermic centrifugation (3000 r?min-1, 10 min 4℃) . The supernatant was collected for determination of its protein content. Lung tissue was obtained for determination of myeloperoxidase (MPO) activity, expression of matrix metallo-proteinase-8 (MMP-8) , CrossLaps protein content (the degradation product of type Ⅰ collagen) and wet/dry lung weight ratio(W/D), and microscopic examination. Pulmonary permeability index was calculated (protein content in bronchoalveolar wash-out fluid / plasma protein concentration) .Results At 4, 6 and 8 h after iv LPS administration (in group 3-5) the MMP-8 expression, crosslaps protein content, MPO activity, W/D and lung permeability index were significantly increased as compared with those in control group ( P

Objective To investigate the character 、difference and clinical meaning of distortion product otoacoustic emissions(DPOAE) and auditory brainstem respons(ABR) in high risk neonates with.Methods DPOAE and ABR were recorded with amplaid MK 22 and GSI60 in 200(400ears) high risk neonates, then compared the results.Results Of 200(400ears) newborns, the presence of DPOAE was 64%(256/400 ears), the presence of ABR was 88.25%(353/400 ears). The co-negativity and co-positivity of DPOAE and ABR were 61.50%(246/400 ears)and 9.25%(37/400 ears). The false negative and false positive of DPOAE were 3.91%(10/256 ears)and 74.31%(107/144 ears).Conclusion The DPOAE test is simple,quick,harmless,sensitive and objective. Because it only reflects the function of the cochlea and has high false positive, we can't make the diagnosis of hearing loss immediately when newborns don't pass the DPOAE test.The ABR test is a reliable method for neonatal hearing screening. The combination of DPOAE and ABR in high risk neonatal hearing screening could increase the accuracy and reliability of the test and retest results.

Objective To investigate the effects of genistein pretreatment on endotoxin-induced acute lung injury in rats and assess the possible mechanism. Methods Thirty-two male Wistar rats weighing 240-280 g were randomly divided into 4 groups ( n = 8, each group) : group Ⅰ control; group Ⅱ genistein; group ? LPS and group Ⅳ genistein pretreatment. The jugular vein was cannulated for administration of fluid and drug. The animals were anesthetized with intraperitoneal 3% pentobarbital 40 mg? kg-1 . In group Ⅰ and Ⅱ Ⅳ normal saline (NS) 1 ml?kg-1 was Ⅳ given 30min after IP NS 1 ml?kg-1 (Ⅰ ) or genistein 50 mg?kg-1 (Ⅱ ). In group ? and Ⅳ LPS 6 ml? kg-1 was Ⅳ given 30 min after IP NS 1 ml?kg-1 (?) or genistein 50 mg? kg -1 (Ⅳ). Four hours after.LPS injection, rats were sacrificed. The lungs were removed for evaluation of histological injury and determination of wet/dry lung weight (W/D) ratio, myeloperoxidase (MPO) activity, malondialdehyde (MDA) content, expression of TNF-f55 mRNA, HO-1 mRNA, TNF-f55, and HO-1. Bronchoalveolar lavage fluid (BALF) was collected for determination of PMN count, protein content, and MPO activity.Results LPS administration induced marked lung injury and significant increases in W/D ratio, MPO activity, and MDA content in the lung tissues, and PMN count, protein content, and MPO activity in BALF. All of these changes were significantly reduced by genistein pretreatment. Genistein also markedly suppressed LPS-induced expression of TNF-a mRNA and protein, and enhanced LPS-induced expression of HO-1 mRNA and protein. Conclusion Pretreatment with genistein has protective effect against endotoxin-induced acute lung injury. The underlying mechanism is via an inhibition of neutrophilic recruitment and activity, a down-regulation in TNF-f55 production, and a up-regulation in HO-1 expression.