Compared to ligand-binding assay( LBA) , liquid chromatography coupled to tandem mass spectrometry( LC-MS/MS) methods could improve specificity, reduce the time of method development and enhance efficiency for quantitation of monoclonal antibodies( mAb) .This review summarizes the application of LC-MS/MS assays to the quantitation of mAb in order to facilitate the research of quantitation of mAb in drug development.

Objective: To investigate the effects of the cyclic uniaxial compressive stress on RKIP and its mRNA in the rat condylar chondrocytes. Methods:The third-passage chondrocytes were harvested from the mandibular condyles of 2-day-old rats, and a cellular compressive stress device (self-made four-point bending system. Patent No: 01129166.4 & 01256849.x) was used to apply stress on cells at 4 000 ?strain ( 0.5 Hz frequency) for 0, 15, 30, 60, 120 and 240 min. The effects of the cyclic uniaxial compressive stress on RKIP and its mRNA in the rat mandibular condylar chondrocytes were examined by REAL-TIME PCR and Western blot. Results:The results showed that the expression of RKIP and its mRNA changed obviously with 4 000 ?strain compressive stress loading, there are reverse tendency in the expression between RKIP and its mRNA. RKIP mRNA significantly increased at 30min (P

Objective To evaluate the the safety and efficiency of precise liver resection for patients with primary liver cancer. Methods 86 patients with primary liver cancer were randomized to receive conventional routine hepatectomy (n=44) or the precise liver resection (n=42). Outcomes were compared between the precise hepatectomy group and the routine group, including, the blood loss, operation time, morbidity and mortality. Results There were significant differences in morbidity rates (7.1% vs. 20.5%; P<0.001), the blood loss [(320±315) ml vs. (613±526) ml;P<0.001) , postoperative alanine aminotransferase (ALT) value (in postoperation 7 d, 82.7 U/L vs.321.7 U/L; P<0.001) and length of hospital stay (12.3 d vs. 18.6 d; P<0.001) between precise hepatectomy and routine groups. The 1 year tumor recurrence rate and 1 year survival rate were 26.2%(11/42) and 78.6% (33/42) in precise liver resection group, 38.6% (17/44) and 65.9%(29/44) in routine liver resection group,with significant difference (P=0.010;P=0.018). Conclusion Precise liver resection is safe and effective in the treatment of liver tumor without much injury to patients.

Objective To discuss whether acupuncture at Neiguan (PC 6) inhibits myocardial hypertrophy via monoamine neurotransmitters.Method Sixty female Sprague-Dawley rats were randomized into a control group, a model group, a sham electroacupuncture (EA) group, and an EA group, 15 cases in each group. The rat models of myocardial hypertrophy were developed by subcutaneous injection of Isoprenaline hydrochloride (ISO) for 14 d. The EA group was intervened by EA at Neiguan (PC 6), and the sham EA group was by EA at a sham acupoint [5 mm away lateral to Neiguan (PC 6). The Electrocardiogram (ECG) of each group was recorded, electron microscope was used to observe the microstructure of the rats’ myocardial tissues, and high performance liquid chromatography (HPLC) was adopted to detect the contents of catecholamines in myocardial tissues.Result The heart rate, R-R interval, ST-elevation, and the contents of catecholamines in rats’ myocardial tissues (adrenaline, noradrenaline, and dopamine) in the model group and sham EA group were significantly different from that in the control group (P<0.05). The heart rate, R-R interval, ST-elevation, and the contents of catecholamines in rats’ myocardial tissues (adrenaline, noradrenaline, and dopamine) of the EA group were significantly different from that of the model group (P<0.05).Conclusion EA at Neiguan (PC 6) can improve myocardial hypertrophy, which is related to the regulation of the topical neurotransmitters; it has provided laboratory evidences for the relationship between Neiguan (PC 6) and heart, i.e. the relationship between acupoints and internal organs.

Objective To investigate the different compatibility of tripterygium wilfordii on its attenuation and synergistic effects through applying Leigongteng decoction and its demolition decoctions on nephrosis rats,and to select the optimizing decoction to treat nephrotic syndrome.Methods 110 Wister rats were divided into 11 groups randomly,which were 9 groups of nephrosis model groups (including Leigongteng group,Leihe group,Leigui group,Leiqi group,Leigongteng ecoction group,Leijin group,Leigan group,prednisone group and nephrosis model group) and 2 blank groups (normal control group and normal Leigongteng group),10 rats in each group.The normal control group and normal Leigongteng group was intravenous injected with 0.65 ml sodium chloride in caudal vein; 9 groups of nephrosis model groups were injected with 0.65 ml adriamycin.The prednisone group was selected for positive control,24h urine protein,total protein,albumin,urea nitrogen,and creatinine were tested with automatic biochemistric meter.Results ALB (27.58±2.62)g/L,Leigan group Compared with(92.60± 18.12) mg in Leigongteng group,the urine protein in Leigongteng diction group(48.60± 11.75) mg,Leiqi group (53.20± 13.21) mg,and Leigan group (56.40 ±16.52) mg were reduced.Compared with the Leigongteng group TPR(58.98±4.84) g/L,ALB (20.98 ± 3.49) g/L,the Leigongteng decoction group [TPR (68.47 ± 5.25) g/L,ALB (28.97± 3.01) g/L],TPR and ALB in Lei-Qi group TPR(68.21 ±4.19) g/L,TPR(67.51 ± 5.42) g/L,ALB (26.56± 1.89) g/L were all elevated.Compared with Leigongteng group chol (1.48 ± 0.65) mmol/L TG (1.70 ± 1.58) mmol/L,chol and TG in the Leigongteng decoction group chol(0.78±0.13)mmol/L,TG(0.56±0.14)mmol/L,Lei-Qi group chol (0.70±0.13) mmol/L,TG (0.65±0.12) mmol/L,and Leigan group chol (0.97±0.33) mmol/L,TG(0.66±0.18) mmol/L were lowered; compared with Leigongteng group,UN (14.20 ± 2.12) mmol/L,Cr (78.10 ± 6.14) μmol/L,UN and Cr in the Leigongteng decoction group UN(7.59±0.78) mmol/L,Cr(39.60±8.33) μmol/L,Leigan group UN (9.48 ± 0.82) mmol/L,Cr(38.30±7.89)μmol/L,and Leijin group UN(9.71±1.33) mmol/L,Cr(41.40±6.45) μmol/L,Leigui group UN(8.30±1.12) mmol/L,Cr(36.90±6.15) μmol/L were all reduced with significant differences (all P＜0.05).The changes of Kidney morphology and ultrastructure pathology in Leigongteng different combination groups were lighter than the control groups.Conclusion Leigongteng decoction group,Leiqi group,Leigan group had synergistic actions,while Leigongteng decoction group,Leigan group,Leijin group,and Leigui group had kidney toxicity-reducing actions.

Objective To investigate the effects of hypoxia on thyroid function in patients suffering from chronic pulmonary heart disease(CPHD).Methods Serum TT 3,TT 4 and TSH in 83 patients with different hypoxia chronic pulmonary heart disease were measured.Observe the changes and relations between TT 3,TT 4,TSH and Pa(O 2).Results The values of TT 3,TT 4 and TSH were (0 71?0 16)?g/L,(62 6?12 3)?g/L and(4 4?0 8)mU/L in patients with mild hypoxia CPHD.(0 49?0 13)?g/L,(33 2?8 5)?g/L and (5 3?0 7)mU/L in patients with moderate hypoxia CPHD.In the patients with severe hypoxia CPHD,they were (0 24?0 07)?g/L,(18 4?4 5)?g/L and (13 1?1 2)mU/L.In control group,they were (0 91?0 36)?g/L,(84 1?15 6)?g/L and (3 7?0 6)mU/L respectively.Pa(O 2) in patients with pulmonary heart disease had a positive relation with TT 3 and TT 4(P

A higher school wants to possess a highlight status in the international educational market,it must launch an attack actively,compete and grab market,anneal ourselves to boost up competition.So,to drive the lasting development of foreign medical students' education in our school,we must progress with time,and deepen reform.

Objective To investigate the gene polymorphism of tumor necrosis factor (TNF) in patients with inflammatory bowel disease (IBD) among the Han nation and its role in the pathogenesis of IBD. Methods Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to analyze the gene polymorphism of TNF? and TNF? in 131 cases of IBD. Results The genotype frequency and allelic frequency of TNF?-308 in ulcerative colitis (UC) patients (15.5% and 8.7% ) were significantly higher than those in control subjects (4.1% and 2.0% respectively, P

Objective To explore the inhibitory effects of SB203580, a specific inhibitor of p38 mitogen activated protein kinase (p38 MAPK) on hyper secretion of airway mucus in mice with asthma. Methods Forty BABL/C mice were randomly allocated into asthma group, SB203580 treatment group, dexamethasone treatment group and control group (sham-challenged group). Mice in SB203580 treatment group were intraperitoneally injected with SB203580 (10mg/kg), while those in dexamethasone treatment group were injected with dexamethasone (1mg/kg) before provocation. The number of goblet cells in small bronchi of all groups was determined after Alcian blue-periodic acid Schiff (AB-PAS) staining. The contents of MUC5AC in bronchoalveolar lavarge fluid (BALF) were evaluated with ELISA asssy. The levels of MUC5AC mRNA expression were determined with reverse transcription-polymerase chain reaction (RT-PCR) assay, and gray-scale analysis was done with image analysis method. Results Compared with the normal group, the number of airway goblet cells, the contents of MUC5AC in BALF and the level of lung MUC5AC mRNA expression in asthma group were raised significantly (P

AIM: To study the effect of platelet-activating factor(PAF) on proliferation of cultured rat airway smooth muscle cells(ASMCs).METHODS: The cells were divided into control group and PAF group. The cells in PAF group were subdivided into four small groups by concentrations of PAF 10 -6 , 10 -7 , 10 -8 , 10 -9 mol?L -1 , MTT assay was used not only to investigate the effects of PAF on proliferation of ASMC but also to confirm the optimal concentration. Flow cytometry and immuneohistochemistry for proliferating cell nuclear antigen (PCNA) were also used to analyse its function on proliferation of ASMC.RESULTS: PAF (10 -6 -10 -9 mol?L -1 ) stimulated the cell proliferation and 10 -7 mol?L -1 PAF reached the maximal effect. The cell percentage of the ASMCs of 107 mol?L -1 PAF subgroup at G_ 0/1 phase (68.67%) was much lower than that of control group (85.57%, P