With the development of funcgonal laboratory teaching,the advantages of integrating laboratory resources have been embodied in someway,improving the students'experiment level and their comprehensive analysis ability.But many new problems have also shown up such as unreasonable curriculum,construction of teaching staff.By reinstalling experiment courses,integrating the experiment teaching staff,and exploring the solution to the existing problems,the teaching quality of functional experiment can be promoted

The use of golden section was proposed as a beneficial supplementary method in development of digital resources based on the long tail theory according to its advantages and disadvantages in development of digital resources in library.Literature download by retrieving subject headings and classification number showed that golden section could be used as a marking point and a supplementary boundary point in development of digital resources based on the long tail theory, thus improving the efficiency, economy and feasibility in development of digital resources with the limited fund.

Objective To investigate the nutritional status and glycemic control level in elderly patients with type 2 diabetes in urban community of Yangpu district in Shanghai.Methods The 24-hour dietary recall method for 3 consecutive days was used to investigate the dietary nutrients intake in 400 patients with type 2 diabetes.Results The ratio of patients with blood glucose level less than 6.1mmol/L was 23.3％ (93/400).The energy intake in patients with type 2 diabetes was low,and only reached 62.81％ of the recommended standard of energy intake for adults.The energy ratio of three diet nutrients (protein,fat and carbohydrate) were 14.41％,33.93％,51.66％ respectively,which showed that the energy ratio of fat was relatively high.The energy ratio of breakfast,lunch and supper dinner were 23.37％,38.05％ and 38.57％ respectively.The average intake of vitamin E,iron,niacin,manganese,phosphorus and cholesterol in elderly patients was more than the recommended standard of adult diet nutrients,and reached 149.6％,138.1％,137.1％,132.5％,127.5％ and 122.0％ of the recommended standard respectively.But the average intakes of protein,fat,carbohydrate,dietary fiber,calcium,zinc,copper,magnesium,potassium,vitamin A,vitamin B1,vitamin B2 and vitamin C in elderly patients were lower than the recommended standard,and reached 77.9％,90.3％,57.1％,50.7％,49.3％,81.4％,75.7％,75.7％,77.5％,77.0％,55.1％,58.1％ and 67.6％ of the recommended standard respectively.Conclusions The status of dietary nutrients intake in elderly patients with type 2 diabetes in Yangpu district of Shanghai is unreasonable,the intakes of dietary fiber,calcium,vitamin B1,vitamin B2 etc.were insufficient,and reasonable diet instruction should be provided for the elderly patients.

Humans ; Integrative Medicine ; methods ; Medicine, Chinese Traditional ; methods

Objective: To discuss the urgent need to improve the handling capacity of the outpatient information system in our hospital under the context that the patient registration is not available after the daily outpatient exceeding about 4,500 people times and that the outpatient demand is rapidly growing. Methods: To analyze all factors which constraint outpatient demands, develop relevant solutions, and update the outpatient information system in phases so as to ensure the normal medical work. Results:After the initial updating, the handling capacity of the system is up to from 4,500 to 13,000 people times, meeting the patient’s need; in the future, the complete updating will lead to a handling capacity to 19,000 people times. Conclusion: The comprehensive consideration shall be given to the building of the hospital information system, including the scalability of the system during its design.

0 05).Conclusions These data showed that infertile women have significantly more mental blocks than infertile men. It is importance that clinicians give psychological support to infertile women in the assisted reproductive treatment, especially alleviating anxiety.

Objective The purpose of this study was to investigate the occupation stress and quality of life among teachers in medical university,and analyze the correlation between the occupational stress and the quality of them.Methods Totally 249 teachers in medical university of Anhui province were investigated with the instruments of the occupational stress scale and the Chinese version of World Health Organization Quality of Life Brief(WHOQOL-BREF).Results The findings showed that:The coefficient of correlation was-0.374 between occupational stress and quality of life in teachers in medical university(r =-0.374,P ＜0.001).Conclusion The score of social relations dimension,the environment dimension and utilization of support have statistical significance with different sex and age of teachers in medical university.There was negative correlation between the occupational stress and the quality of life in teachers in medical university.

AIM: To detect the protein expression of protein gene product (PGP9.5) in cervical carcinoma samples, and to explore its relationship with clinicopathological features and prognosis of cervical carcinoma patients.The potential value of PGP9.5-siRNA in the treatment of cervical cancer was preliminarily investigated in vitro.METHODS:The clinical data of cervical cancer patients ( n=180 ) who received surgical treatment in Department of Gynecology of Tianjin Fifth Central Hospital and Tianjin Central Hospital of Gynecology and Obstetrics from January 2008 to June 2015 were retrospectively analyzed.Immunohistochemical staining for PGP9.5 in all pathological specimens from cervical cancer biopsy was performed.The patients were divided into high expression of PGP9.5 group and low expression of PGP9.5 group.The relationship between PGP9.5 expression and clinicopathological parameters, such as age, HPV infection, path-ological grade, tumor diameter, lymph node metastasis, depth of invasion and clinical stage, were analyzed.The overall survival was analyzed by Kaplan-Meier method and the log-rank test.The PGP9.5-siRNA, NC-siRNA, PGP9.5 eukaryotic expression plasmid and empty vector were transfected into the SiHa cells, and the effects of PGP9.5 expression on the abili-
ties of colony formation and cell invasion were determined by Western blot, colony formation assay and Transwell experi-ment.RESULTS:The relationships between the expression level of PGP9.5 and patients’ pathological characteristics in-cluding grade, tumor size, lymph node metastasis, depth of invasion, vascular involvement and clinical stage were statisti-cally significant ( P<0.05) .The overall survival rates of 3 and 5 years in PGP9.5 high expression group were significantly lower than those in PGP9.5 low expression group ( P<0.05 ) .Compared with control group, the protein expression of PGP9.5, the number of colony formation and the number of invasive cells in si-PGP9.5 group were significantly decreased. The protein expression of PGP9.5, the number of colony formation and the number of invasive cells in PGP9.5 group were significantly increased.CONCLUSION:Over-expression of PGP9.5 protein indicates poor prognosis of cervical cancer, which may be a good predictor for the prognosis of cervical cancer patients.Inhibition of PGP9.5 expression may be an ef-fective way of gene therapy for cervical cancer.

AIM:To evaluate the role of inhaling N 6-phenyl-2R-isopropyl-adenosine (R-PIA) on the airway resistance and pulmonary compliance of experimental asthmatic guinea-pigs. METHODS: Experimental model of asthmatic guinea-pigs were made. Inhaling R-PIA 5 mg/mL( 5 mg R-PIA in 1 mL 0.9% saline). The airway resistance, pulmonary compliance and NO- x, cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP),tumor necrosis factor (TNF) in bronchoalveolar lavage fluid (BALF) were observed. RESULTS: Compared with asthma group, after inhaling R-PIA, the airway resistance of experimental asthmatic guinea-pigs increased ( P 0 05). CONCLUSION: Inhaling R-PIA could constrict the airway of experimental asthmatic guinea-pigs.

Objective:To study the perioperative anticoagulant therapy for patients in prevention of moderate or high risk of pulmonary embolism(PE)after general surgery.Method:1146 patients who had undergone major general surgery were divided into a non-anticoagulant therapy group and an anticoagulant therapy group.There were 743 patients without the anticoagulant therapy during Jan.2005 to Aug.2008,a preventive anticoagulant therapy was performed on 403 patients who recived 5 000IU(142 patients at moderate risk of PE)or 10 000IU(261 patients at high risk of PE)low molecular weight heparin(LWMH)subcutaneous injection after major general surgery during Jan.2006 to Nov.2008.Result: Five cases died and 2 survived among 7 PE patients.Hemorrhage occurred in 3 out of 403 cases(0.7%),but no PE occurred among 403 patients administered with the LWMH anticoagulant therapy.The incidence of PE in the patients reciving LWMH decreased from 0.9%to 0(P