1.Study on efficacy accompanied by side effects of water extraction components of Evodiae Fructus based on syndrome model.
China Journal of Chinese Materia Medica 2015;40(14):2753-2759
The range of effective dose and mechanism of abirritation about water extraction components of Evodiae Fructus on the stomach cold syndrome model in mice were preliminary studied. The method of stomach cold-syndrome model in mice was built, which were administrated with different doses water extraction components of Evodiae Fructus, observing abirritation and toxicity by the classical hot plate method, detecting the level of ALT, AST, PGE2, NO, NOS, MDA, SOD, GSH, GSH-Px, BUN, CR in serum and ALT, AST in hepatic tissue, and recording toxicity symptoms in mice according to the list of relevant toxicity reaction. The water extraction component of Evodiae Fructus has obvious analgesic action after administration 30 min, arriving peak effect after administration 60 min, showing certain "dose-time-toxicity" relationship. ALT and AST levels in mice serum and liver tissue enhanced; PGE2, MDA, NO, NOS enhanced in mice serum; SOD, GSH, GSH-Px reduced; the BUN, CR levels was no significant alteration; liver weight/ body weight enhanced; kidney weight/body weight was no significant alteration. The a irritation mechanism of volatile oil of Evodiae Fructus was connected with suppressing pain transmitters release, per oxidative damage mechanism and NO damage, which also induced hepatotoxicity and the mechanism of hepatotoxicity is main lyoxidative damage, showing certain "dose-time-toxicity" relationship in accordance to hepato-toxicity injury.
Analgesics
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pharmacology
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Animals
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Body Weight
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drug effects
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Chemical and Drug Induced Liver Injury
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etiology
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Disease Models, Animal
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Dose-Response Relationship, Drug
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Evodia
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toxicity
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Female
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Medicine, Chinese Traditional
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Mice
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Plant Extracts
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pharmacology
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toxicity
2.Study on efficacy and accompanying toxic and side effects of volatile oil of Evodia Fructus based on stomach cold syndrome model.
China Journal of Chinese Materia Medica 2015;40(19):3838-3844
OBJECTIVETo preliminarily study the effective dosage range and mechanism of the abirritation of volatile oil of Evodia Fructus on the stomach cold syndrome model in mice, and discuss the correlation between its accompanying toxicity and oxidative damage mechanism, in order to provide the experimental basis for explaining the efficacy-syndrome-toxicity correlation.
METHODThe stomach cold-syndrome model in mice was induced by the classic hot plate test by orally administrating with different doses of volatile oil of Evodia Fructus, in order to observe its abirritation and companying toxic and side effects and detect serum ALT, AST, PGE2, NO, NOS, MDA, SOD, GSH, GSH-Px, BUN, CR and hepatic ALT, AST. The companying toxic symptoms in mice were recorded in toxic reaction integral table.
RESULTVolatile oil of Evodia Fructus had an obvious analgesic effect at 30 min after the oral administration and reached the peak effect at 60 min, with certain "dose-effect" and "time-effect" relations, rises in serum and hepatic ALT and AST levels, serum PGE2, MDA, NO and NOS and hepatic indexes, decreases in SOD, GSH and GSH-Px and no notable change in BUN, CR levels and kidney weight/body ratio. Conclusion: The abirritation mechanism of volatile oil of Evodia Fructus was related to the inhibition of pain transmitter release, peroxidative damage and NO damage, which is accompanied by certain hepatotoxicity, mainly mainly oxidative damage, with a concurrent "dose-time-toxicity" relationship.
Animals ; Drugs, Chinese Herbal ; administration & dosage ; toxicity ; Evodia ; chemistry ; toxicity ; Female ; Fruit ; chemistry ; toxicity ; Humans ; Liver ; drug effects ; metabolism ; Mice ; Oils, Volatile ; administration & dosage ; toxicity ; Oxidative Stress ; drug effects ; Stomach ; drug effects ; metabolism ; Stomach Diseases ; drug therapy ; metabolism
3.Effects of Environmental Lead Exposure on Immune System in Preschool Children
Li SUN ; Zhengyan ZHAO ; Rong LI
Journal of Environment and Health 1992;0(02):-
Objective To explore the effects of environmental lead exposure on immune system in preschool children. Methods The blood lead levels of 217 preschool children were determined by graphite furnance atomic absorption spectrophotometry. The distribution of T_lymphocyte subsets: CD 3+,CD 3+CD 4+,CD 3+CD 8+, B cells (CD 3- CD 19+), NK cells (CD 3-CD 16+CD 56-) were analyzed by flow cytometer, the levels of serum immunoglobulin G and immunoglobulin M were determined by scattering turbidimetry, the levels of serum immunoglobulin E were examined with ELISA. Results The mean level of blood lead of 217 preschool children was (0.46?0.27)?mol/L(range:0.11~2.71 ?mol/L). The blood lead levels of 63 preschool children were ≥0.48 ?mol/L. 38 preschool children among 63 preschool children with blood lead level of ≥0.48 ?mol/L were selected as the high_blood_lead group, 35 preschool children with blood lead levels of 0.05). Condusion The blood lead levels of ≥0.48 ?mol/L presented adverse effects on the T_lymphocyte subsets.
5.Isolation, Purification and Structural Identification of Polysaccharide from Cordyceps Sinensis
Rong LI ; Shujuan SUN ; Xiaolu JIANG
China Pharmacist 2015;(3):407-409,419
Objective: To isolate and purify the polysaccharide from Cordyceps sinensis, and analyze its structure. Methods:Cordyceps sinensis was cultured by a liquid fermentation method. A water-extraction and alcohol-precipitation method was applied to ex-tract polysaccharide from Cordyceps sinensis fermentation liquor (EPS) and polysaccharide from Cordyceps sinensis mycelium (IPS). Sephadex gel chromatography was applied to isolate and purify the polysaccharide. The purity and relative molecular weight of the poly-saccharide were determined by a gel filtration method. The monosaccharide composition of the polysaccharide was identified by GC. The content of uronic acid was analyzed by sulfuric acid carbazole method. Results: The analysis results showed that the molecular weight of EPS was 78kDa. The content of polysaccharide and uronic acid was 94. 8% and 6. 0%, respectively. EPS was composed of mannose,glucose and galactose with the molar ratio of 4. 5∶8. 0∶1. 0. The molecular weight of IPS was 42kDa. The content of polysac-charide and uronic acid was 92. 5% and 4. 5%, respectively. IPS was composed of mannose,glucose and galactose with the molar ratio of 2. 8∶3. 0∶1. 0. Conclusion:Both polysaccharide EPS and IPS in Cordyceps sinensis are heteropolysaccharide.
6.The significance of anti-p68 antibody in rheumatoid arthritis
Xiaoyun SUN ; Rong MU ; Zhanguo LI
Chinese Journal of Rheumatology 2003;0(09):-
Objective To evaluate the diagnostic specificity and sensitivity of anti-p68 antibody in rheumatoid arthritis (RA), and to analyze the relationship between anti-p68 antibody and the activity of RA . Methods Anti-p68 antibody was tested in the serum of 183 RA patients and 195 controls by indirect immunofluorescence (IIF). The clinical characteristics of RA between anti-p68 positive group and negative group were compared. Results ① The positive rate of anti-p68 antibody was 67.8% (124/183) in RA, 14.9% (17/114) in other rheumatic diseases and none in normal controls (0/81). Anti-p68 antibody was different from other auto-antibodies found in RA. ② Its sensitivity was 67.8% and specificity was 91.3% for RA, positive prediction rate was 87.9% and negative prediction rate was 75.1%. ③ There was statistic difference between the positive group and negative group in disease duration, RF and degree of X-ray changes. Conclusion ① Anti-p68 antibody is a new and specific serum maker for diagnosis of RA. ② The antibody is correlated with disease duration, RF and X-ray changes.
7.Diagnostic significance of combined detection of rheumatoid factor and anticyclic citrullinated peptide antibodies in rheumatoid arthritis
Rong MU ; Xiaoyun SUN ; Zhanguo LI
Journal of Peking University(Health Sciences) 2003;0(05):-
Objective:To evaluate the significance of the combination of rheumatoid factor (RF) and anti-filaggrin antibodies (AFAs) in the diagnosis of rheumatoid arthritis (RA). Methods:Sera from 266 RA patients and 186 controls were studied. RF and some kinds of AFAs (including antikeratin antibodies, anti-perinuclear factor and anticyclic citrullinated peptide antibodies) were detected using immunofluorescence and ELISA respectively. The distributions of these antibodies were compared to determine the significance of the combination of RF and AFAs. Results:The sensitivity and secificity of RF for the diagnosis of RA were 65.8% and 81.8% respectively, as compared with those of anti-CCP or RF positive were 86.5% and 80.6% respectively. Only a slight change was found in the secificity and negative predictive value between them. RF could not be detected in 91 patients while 64.8% of them could be found to have at least one kind of AFAs. Conclusion:AFAs, particular anticyclic citrullinated peptide antibodies, could be helpful for the diagnosis of RA especially in RF-negative patients.
8.Influence of Long-Term Inhaled Glucocoticoids on Soluble Intercellular Adhesion Molecule-1 of Bronchial Asthmatic Children
ding-rong, ZHANG ; rong-jun, LIN ; wen-wei, WU ; yan-shi, SHAO ; li-rong, SUN
Journal of Applied Clinical Pediatrics 2006;0(18):-
Objective To explore influence of long-term inhaled glucocoticoids(IGS) on soluble intercellular adhesion molecule-1(sICAM-1)) in children with bronchial asthma.Methods Enzyme-linked immunosorbent assay(ELISA) method was used to detect the serum sICAM-1 level in 36 healthy children and 29 children with bronchial asthma(untreated and post-treated for 3,6 and 12 months).Results 1.Serum sICAM-1 level was significantly elevated in children with asthma and significantly higher than that in normal control group(P
10.Ring chromosome 14 syndrome: a case report.
Rong LI ; Zheng-yan ZHAO ; Li-ying SUN ; Xingbao ZHENG
Chinese Journal of Pediatrics 2004;42(12):956-957