Objective To acknowledge the effect of T-lymphocyte subset on lymph node micrometastasis in esophageal cancer patients by the study of the correlation between T-lymphocyte subset and micrometastasis.Methods CD3+、CD4+、CD8+and CD4+/CD8+ were examined in esophageal cancer patients under thoracotomy by flow cytometry,and the micrometastasis were examined by immunohistochemistry in lymph node that were diagnosed as negative for metastases by routine histopathological analysis.Two groups were divided by with or without micro-metastasis,T-lymphocyte subset were compared with two groups.Results CD3+,CD4+ and CD4+/CD8+were significantly lower in group with micrometastasis than in group without micrometastasis,CD8+ was significantly higher in group with micrometagtasis than in group without micrometastasis.Conclusion T-lymphocyte subset is closely associmed with micrometastasis and low CD3+,CD4+ and high CD8+ T-lymphocyte is associated with poor cancer-specific survival.

Objective In order to provide the reasons for local injecting Xiaozhiling to treat malignant tumors. Methods 10 mice were subcutaneosly injected with S 180 Tumor cell to create tumor models and then Xiaozhiling 10ml/kg were intratumorly injected for 3 times.Finally,observation and comparison were made for the pathologic changes under optic and electronic microscopies. Results The results showed that Xiaozhiling had strong antitumor effect on S 180 tumor(adquate rate was 37.3%).Pathalogical exemination revealed that the tumor tissue necrosed obviously,some wesslincataneous cells breaked up, red blood cells extravasation,micro blood clots formed.No changes occurred in the control group. Conclusions Xiaozhiling could be a new drug for clinical treatment of malignant tumor.

Objective: To observe the effect of Ruxiankang (RXK) Capsule (Radix Bupleuri, Poria, Rhizoma Atractylodis macrocephalae, Radix Angelicae Sinensis, Radix Paeoniae Alba, Rhizoma Cyperi, etc.) on mammary gland and sex hormone of rat with mastoplasia. Methods: After benzoate estradiol and progestogen were injected into rats for 30 days the mastoplasia model was made, the model rats were treated with tamoxifen and RXK Capsule for 30days, and injected estradiol at the same time. Results: RXK Capsule and tamoxifen could significantly inhibit HMG rat's mammary gland swell, reduce quantity of lobule alveolus in mammary glands and secretion ( P

Objective To investigate the impacts of γ-ray radiation on gene expressions of peripheral blood lymphocytes in SD rats,and to screen differential expression genes and biological pathways closely related to radiation injury.Methods The differential gene expression of peripheral blood lymphocytes in SD rats was selected by microarray at 6 h after 2 Gy 60Co γ-ray exposure in vitro and in vivo.Bioinformatics analysis of the differentially expressed genes was performed by using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases.Real-time quantitative PCR was applied to verify the screen results of the microarray.Results Fifty-five genes with three times over-expressed level were screened out from the radiation groups both in vitro and in vivo and they were involved in 6 signaling pathways.There were two differentially expressed genes of microarray assay were verified by PCR assay.Conclusions Differential expressed genes in the peripheral blood lymphocytes of SD rats could be induced by γ-ray radiation and they cooperatively contributed to a variety of biological processes.

Adult ; Aged ; Female ; Gastrointestinal Diseases ; Humans ; Male ; Middle Aged ; Paresthesia ; Pharyngeal Diseases ; Upper Gastrointestinal Tract

ObjectiveTo investigate the therapeutic effects of modified constraint-induced movememt therapy(mCIMT) on upper extremity motor function of stroke patients with hemiparasis, and to compare the effects of mCIMT with those of conventional rehabilitation.MethodsThirty stroke patients with hemiparesis were divided into mCIMT group and control group (conventional rehabilitalion), 15 cases in each group. The mCIMT group received mCIMT (by shaping) in the affected extremity without restriction of movement of intact upper extremity, 6 h a day, five times a week, for two weeks while the control group was treated with traditional rehabilitation in upper-limb and hands functional exercises. Motor activity logs(MAL) score and simple test for evaluating hand function(STEF ) score were measured in two groups before treatment and 2 weeks, 1 month and 3 months after treatment.ResultsThere were improvement in MAL and STEF scores in both mCIMT group and control group 2 weeks after treatment compared with pre-treatment(P<0-01), and the scores of MAL and STEF on post-treatment 1 month and 3 months were higher than those of pre-treatment in mCIMT group (P<0-05, P<0-01), but there was no significant difference in MAL and STEF score between pre-treatment and post-treatment 1 month and 3 months in control group(P>0-05).ConclusionSignificant improvement in the affected hand function could be achieved with modified constraint-induced movement therapy in stroke patients, better than conventional rehabilitation therapy.

ObjectiveTo explore the relationships of driving coping styles with driving behaviors and road accidents of drivers.MethodsThree hundreds and thirty-seven drivers were randomly surveyed by Driving Coping Questionnaire ( DCQ),Driver Behavior Questionnaire (DBQ).Results ( 1 ) Except avoidance coping style,confrontive coping and emotion-focused coping were correlated positively with the three driving behaviors ( r =0.18 ～ 0.56,P ＜ 0.01 ),and positive appraisal coping had negatively correlations with them (( r =-0.34 ～-0.41,P＜0.01 ).(2)Positive appraisal coping,confrontive coping and emotion-focused coping could predict 33.5％ of error behaviors and 23.5％ of slip behaviors.And confrontive coping and positive appraisal coping could predict 40.2％ of speeding and violation behaviors.(3) Compared with safety drivers,accident drivers had significantly differences in confrontive coping in mild and moderate crashes( t=-2.75,2.80,P＜ 0.01 ).ConclusionDriving coping styles are the important factors influencing drivers' behaviors and road safety.

Objective To explore the characters of non-professional drivers' stress. Methods Two hundred and sixty-four non-professional drivers in Chengdu city were randomly investigated by Driver Stress Inventory(DSI). One hundred and five professional drivers were as control group. Results ①Compared with professional drivers,non-professional drivers scored higher in thrill seeking, cognitive fatigue and aggression (non-professional drivers :4.63±1.49,5.12±1.56,5.65±1.42; professional drivers: 3.60 ± 2.04,3.97 ± 2.02,4.56 ±1.84, P ＜ 0.01 ) ,and lower in hazard monitoring, driving anxiety and physiological fatigue (non-professional drivers:7.04±1.48,6.44±1.50,5.78±1.75; professional drivers:7.89 ± 1.45,6.99 ± 1.28,6.45 ± 2.37, P ＜0.01 ). ②Non-professional drivers' stress had significant differences in gender, age, marital state and driving experiences (P＜0.05). The male drivers scored higher in thrill seeking, aggression and physiological fatigue (male:4.79±1.53,6.78±1.46,5.94 ± 1.80; female:4.32±1.37,5.80±1.37,5.50 ± 1.61, P ＜ 0.05 ). Compared with other age driver groups, the 20 ～29 driver group scored higher in cognitive fatigue (5.44 ± 1.38 ), and lower in hazard monitoring(6.77±1.44) ,driving anxiety (6.03 ±1.46). Compared with unmarried drivers, married non-professional drivers scored higher in hazard monitoring and driving anxiety ,and lower in cognitive fatigue( married drivers:7.23±1.43,6.65±1.48,4.88±1.6; unmarried drivers :6.77±1.47,6.06±1.43,5.59±1.36, P ＜0.05 ). The non-professional drivers who drove more than 20 thousand kilometers scored higher in driving anxiety (6.89±1.41). Conclusion The non-professional drivers' stress isn' t optimistic, and influenced by their gender,age, marital state and driving experiences. Stress management should pay attention to them.

Objective To evaluate surgical therapy for recurrent rectovaginal fistula.Methods In this study.two patients were treated by endorectal advancement flap repair and one patient was treated by vascular pedicled segment of small bowel.Results All patients were cured and followed up from 4 to 20 months.During the period of follow-up there was no recurrence.Conclusions The procedures and timing of operation are important factors for a successful repair.Both the endorectal advancement flap and patch of intestine provide an effective methods in repairing recurrent rectovaginal fistula.

OBJECTIVE:To improve hydroscopicity and fluidity of traditional Chinese drug extractum by spray drying and to improve affixes to the wall of the drier during drying for providing reasonable relative humidity of production.METHODS:The compounding of adjuvant and the technology were optimized by orthogonal experiment,and the hydroscopicity and fluidity of powdered extract were investigated.RESULTS:The hydroscopicity of traditional Chinese drug extractum can be greatly decreased by adding 3% gum arabic and 7% ?-CD.The optimum technological conditions were as follows:extractum density of 1.10g? mL-1,temperature of intake airflow of 170℃,atomization pressure of 0.5Mpa,and feed material speed of 400mL? h-1 by orthogonal experiment.The critical relative humidity of the optimum formula was 64%.CONCLUTION:The problems of time consuming and moisture absorption of traditional Chinese drug extractum existed in the traditional old technology can be improved in this new technology.