Adult ; Aged ; Aged, 80 and over ; Anti-Infective Agents ; pharmacology ; therapeutic use ; Cross Infection ; drug therapy ; microbiology ; Female ; Hospitalization ; Humans ; Inpatients ; Male ; Middle Aged ; Pneumoconiosis ; microbiology ; Pneumonia ; drug therapy ; microbiology ; Retrospective Studies

OBJECTIVE To investigate infection of Chlamydia trachomatis(CT) and Ureaplasma urealyticum(Uu) in patients with nongonococcal urethritis(NGU). METHODS C.trachomatis was determined by fast immune method of antigen-antibody.U.urealyticum was detected by liquid culture medium. RESULTS Among 320 cases the positive rate of C.trachomatis,and U.urealyticum was 36.25% and 43.75%,respectively.and that of complicated infection was 20.00%.There were significant differences between men and women in C.trachomatis and U.urealyticum infection(P

Objective　To explore left atrial(LA) structur e and function changes in 86 cases of essential hypertension disease (EH) and ef fects of age,gender,hypertension duration,hypertension stage and left ventricula r function on them.Methods　 Using M-mode echocardiogram to detect LA diameter, Usi ng ultrasonic acoustic quantification (AQ) to detect LA area,LA systolic and dia stolic function,LV systolic and diastolic function. Results　①Compared with normal group, LA diameter and LA area we re larger in EH group, LA systolic and diastolic function were impaired. ② Gend er had no effect on LA structure and functione in EH patients; LA diameter and L A area dilated with age, LA systolic function was impaired with age. ③ Hyperten sion duration had no effect on LA structure and function in EH patients. ④LA d iameter and area dilated with hypertension stage. With the development of hypert ension, LA diastolic function was first impaired,then LA systolic function was a lso impaired.⑤LA structure and function had signifficant relationship with LV d iastolic function, LA might dilate and LA systolic function might be impaired wi th LV diastolic function damage.Conclusions　LA diameter and LA area are dilated and LA function i s impaired in EH patients, which changes become more signifficant with age. LA s tructure change and systolic function impairment have a relationship with LV dia stolic dysfunction.

0.05).No correlation was found between phosphate concentration and dft, dfs or CSI. Conclusions: There is no correlation between phosphate conc entration and caries status.

Objective To investigate allele frequency distributions and haplotype polymorphism of ten Y-chromosome short tandem repeats (STR) loci of Han population in Zhuhai area and evaluate their applicability to forensic cases. Methods DYS393, DYS19, DYS389 Ⅱ, DYS390, DYS391, DYS389 Ⅰ, DYS439、DYS438、DYS392 and DYS385 loci were amplified with Y-plex~(TM) PCR amplification Kit and analyzed by ABI 310 genetic analyzer. 200 unrelated male individuals of Han nationality in Zhuhai were recruited for determination of allele frequencies and haplotype data. Results 5, 6, 6, 5, 4, 5, 5, 5, and 7 alleles were found in 9 of the 10 Y-STR loci, respectively and 44 haplotypes were detected at DYS385 locus. The range of gene diversity (GD) was determined between 0.3904 for DYS391 and 0.9497 for DYS385. Total 161 haplotypes were found at the 10 Y-STR loci, of which 134 occurred once, 20 occurred twice, 3 occurred three times, 3 occurred four times, and 1 occurred five times. The cumulative GD was 0.9948. Conclusion The 10 Y-STR loci are highly polymorphic and suitable for forensic individual identification and paternity testing in Zhuhai Han population.

The article describes the current situation of training mode of community nurses in community health service institutions in Hubei province from several aspects,including training object,training target,training base,teaching staff,training organization and government,training method and time,training curriculum,training effect evaluation and certification.The article suggests a multitude of factors influencing the training quality,such as formal training,dominant job training,and monotonous community training content and less training time.Overall,the government should be done something to increase government support and funding and accelerate the community nursing services and community nursing education.It is also essential to establish community nursing personnel training mechanism,improve and perfect the community health personnel training system,increase the construction of community nursing practice base and facilities and strengthen clinical practice skills.

Luminol dependent chemilumi-nescence (CL) method was used to observe effect of nifedipine on polymorphonuclear leukocytes (PMN) oxygen metabolism in coronary heart disease (CHD). Studied subjects including 24 cases of coronary heart disease non using nifedipine, 26 cases of coronary heart disease using nifedipine group (10.mg tid on 3~7 days), 30 cases of normal drug-free subjects.The results showed that: 1. PMN-CL in using nifedipine CHD group was sigrlifficantly lower, its PMN- CL backgroud, peak value andphargocyte index was signifficantly lower than that in non using nifedipine CHD group. 2. PMN-CL peak value and phargocyte index in using nifedipine CHD group had no signifficantly differences compared with normal group. 3. Parameters of PMN- CL in non using nifedipine CHD group were signifficantly higher than that in normal group.

HRP solution was injected into the dorsal commissural nucleus (DCN) of segment L_6 or S_1 of the spinal cord and laterodorsal tegmental area(TLD)——take the Barrington's nucleus as its center and lateral parabrachial nucleus (PBL) of the rostral pons in different individuals of the rats. After HRP was injected into the DCN, labelled neurons and dense terminals were found in Barrington's nucleus, and labelled terminals appeared in the PBL. When the unilateral TLD was injected the labelled cells and terminals were found in the DCN and bilateral intermediate zone (IM), and formed a band of labelled neurons and terminals. When the PBL was injected the labelled neurons were observed in the DCN and bilateral IM. A few labelled neurons were found in lamina Ⅰ in the latter two experimental groups.Based on the present and previous studies, the authors got the following understandings:1. Morphylogically, the present study for the first time demonstrated that, the micturition reflex arch through the pontine consists of following parts: the primary afferent neurons of the bladder→secondary relay neurons of DCN→Barrington's nucleus→IM (mainly IML) parasympathetic preganglionic neurons→parasympathetic postganglionic neurons.2. According to the present and previous researches, the authors conjecture that, the secondary fibers of visceral sensation of the pelvic organs originate from the neurons of DCN, IM and lamina I and project into the PBL.3. According to the facts mentioned above, we presume that the DCN and bilateral IM constitute a complex and named it the 'visceral field', and which is closely associated with the pelvic organs. This field has widespread connections with the peripheral efferent and afferent nerves. On the other hand, i t contains a lot of relay neurons projecting into Barrington's nucleus and PBL and receives the terminals of descending fibers of the neurons of the Barrington's nucleus. The descending fibers also project into the Onuf's nucleus.In addition, the present study disscussed the complicated functions of the DCN systematically.

Objective We summarized the clinical experience of modified ileal ureter substitution for treating long segment ureteral defection.Methods We retrospectively analyze the clinical data of 2 patients with long segment ureteral defect who treated with Yang-Monti ileal ureter substitution between March 2015 and November 2015.One 75 years old male patient was diagnosed as upper ureteral malignance and solitary kidney.The length of defection from renal pelvis to bladder was 22 em.His serum creatinine was 100 μmol/L,blood urea nitrogen was 5.7 mmol/L,serum chloride was 98 mmol/L.Another one 41 years old female patient was diagnosed as middle and lower ureteral iatrogenic injury.The traumatic length was 15 cm.Her serum creatinine was 70 μmol/L,blood urea nitrogen was 5.1 mmol/L,serum chloride was 100 mmol/L.they were both treated by Yang-Monti ileal ureter substitution.The ileal intestinal segment was used for the ureteral replacement,which were more than 15 cm to the ilealcecum.The length of intestine was 10.0 cm and 7.5 cm,respectively.The ileal mesentery was preserved.After closing the mesangial hiatus,the ileal segment was pull into the retroperitoneal space and pulling out via descending colonic mesangial window.The ileal segment was divided into three parts,which was 2.5 to 3.0 cm in each part.Each part was opened via long axis and then rotated 90 degree.The 4-0 absorable suture was used to suture the edge of each intestinal part continuously.The sutured intestine was re-tubularized,using 4-0 absorable suture and the F16 catheter was used as the tube model.The length of reconstructed ureter was 22 cm and 18 cm,respectively.The neo-ureter was re-anastomosed with renal pelvis and bladder wall.Two F6 double J stents were placed in the neo-ureter.Results The operative time was 160 min and blood loss was 200 ml in the first case.In the second case,the operative time was 180 min and blood loss was 220 ml.No significant complications were noticed intra-operation and post-operation.Six months after operation,the male patient's serum creatinine was 112 pmol/L,blood urea nitrogen was 6.1 mmol/L,serum chloride was 106.0 mmol/L and electrolytes were normal.In another patient,serum creatinine was 79 μmol/L,blood urea nitrogen was 5.9 mmol/L and serum chloride was 103.0 mmol/L.The GFR was 24.9 ml/min and 22.1 m]/min 3 and 6 months after operation,respectively.Ureteral obstruction wasn't detected on IVU images 3 months after operation.Conclusions For patient with long ureteral defect,which cannot be replaced by other urinary tissue,YangMonti ileal ureter substitution is one of the optional modalities.As a new technique of ureteral substitution,Yang-Monti ileal ureter substitution is simple and fewer complications and can improve the quality of life in patient compared with traditional ureteral substitution.

The aim of the study is to investigate the effect of nardosinone (Nar) on neuronal injury induced by oxygen-glucose deprivation (OGD) in primary cortical cultures isolated from embryos at gestational day 14. MTT method was used to determine the dosage regimen of Nar in primary neuronal cultures and observe the influence of Nar on the neurons suffering OGD; Western blotting analysis was used to detect expressions of protein kinase A (PKA), Ras related protein 1 (Rap1), mitogen-activated protein kinase kinase 1 (MEK1) and phospho-extracellular signal-regulated kinase 1/2 (p-ERK1/2) of OGD-injured or uninjured primary cultured neurons after Nar treatment. Results showed that Nar (50 and 100 micromol x L(-1)) improved the cell viability during OGD damage (P < 0.01) and increased the expression of PKA, Rap1, MEK1 and p-ERK1/2 in injured neurons. Additionally, elevations of PKA, Rapl, MEK1 and p-ERK1/2 in uninjured neurons were caused by Nar (50, 100 and 200 micromol x L(-1)) with a dose-dependent tenclency as well (P < 0.01). In conclusion, Nar could protect against the neuronal injury exposed to OGD, which may be relevant to the promotion of PKA and ERK signaling pathway.