Post-operative pancreatic fistula(POPF)is the most common and severe complication after pancreaticoduodenectomy,which may have significant impacts on curative effects and prognosis.It has been shown that POPF is not only closely associated with texture of pancreatic parenchyma,diameter of pancreatic duct and tumor site,but also affected by surgeon's experience and surgical techniques.Double layer pancreaticojejunostomy and external pancreatic duct stent may be beneficial to decrease POPF.

Vildagliptin is a potent and selective inhibitor of dipeptidyl peptidase-4 (DPP-4), which can prevent the cleavage and inactivation of glucagon-like peptide-1 (GLP-1), and thus increases the plasma level of intact GLP-1. Such action promotes the insulin secretion from β cell and suppresses inappropriately glucagon secretion from α cell in a glucose-dependent manner. Vildagliptin used as monotherapy or in combination with other antidiabetic drugs can effectively reduce HbA1C, fasting and postprandial plasma glucose, and improve islet β cell and α cell function in type 2 diabetes mellitus patients with extremely few hypoglycemia and comparable adverse effects with placebo, but no weight gain. The unique drug action, reliable clinical efficacy, and favorable safety and tolerability of vildagliptin make it a novel oral antidiabetic drug for the treatment of type 2 diabetes mellitus.

Objective To observe the effects of anti lipopolysaccharide (LPS) immunoglobulin Y (IgY) on LPS in vitro . Methods The direct in vitro neutralization of different concentrations of IgY and LPS was conducted for the detection of the remained amount of LPS to observe the dose effect relationship. The primary cultured human umbilical vein endothelial cells (HUVECs) were cultured for 48-72 h after addition of certain concentrations of fluorescein isothiocyanate LPS (FITC LPS) and IgY. Changes of the fluorescent intensity in HUVECs were observed at different time points. After addition of certain concentrations of LPS and IgY for incubation with HUVECs for 4, 8, and 12 h, the changes of lactate dehydrogenase (LDH) activity were measured. Results The dose effect relationship was satisfactory when the ratio was 16︰1. At 4, 8, and 12 h, fluorescent intensity was somewhat strong in FITC LPS group, but weak in IgY group, especially at 8 and 12 h. The LDH activity in medium increased obviously after incubation for 8 h in LPS group ( P

Zebrafish has been widely used as an important model system in research fields of genetic and develop -mental biology over the past 20 years.Similar to the mammalians and other vertebrate animals , zebrafish also has various tissue barriers.In recent years, more and more important progress of tissue barrier studies have been achieved using ze -brafish as in vivo model, such as blood-brain barrier.These findings contribute to the understanding of the mechanisms of diseases caused by the disorders of physiological barriers .It also helps with the modulation of the permeability of tissue bar-riers for drug delivery .This review summaries recent progress of zebrafish applications in the study of tissue barriers , such as blood-brain barrier, blood-retina barrier, epidermal barrier, etc.

Objective:The present study discussed the clinical diagnosis and treatment of pancreatic body and tail carcinoma. Methods:The data of 52 patients with pancreatic body and tail carcinoma treated in Tianjin Medical University Cancer Institute and Hospital from January 2008 to December 2012 were reviewed retrospectively. The data included historical materials of perioperative examination and therapy. The data of 49 cases were reviewed retrospectively, and the median survival was calculated by the Kaplan-Meier method. The effects of the clinicopathologic parameters on the prognosis of patients with pancreatic body and tail carcinoma were examined by the log rank test. Results:Thirty-eight patients underwent exploratory surgery among which 24 had surgical resection, and the standard procedure was distal pancreatectomy plus splenectomy. The tumor staging was stage I in five patients (13.16%), stage II in nineteen patients (50%), stage III in five patients (13.16%), and stage IV in nine patients (23.67%). The median survival time was 18.0 ± 1.23 months for patients who received radical resection and 10.0 ± 2.71 months for patients who underwent nonspecific treatment or palliative therapy. The radical resection was associated with a longer survival period than the nonspecific treatment or palliative therapy (P<0.01). Conclusion:Early diagnosis is the key to achieving long-term survival. The radical resection plays an important role in improving the surgical treatment.

Objective:To assess the analgesic effects and side effects of buprenorphine sublingual tablet in dental treatment.Methods:500 cases for dental treatment were selected.Before management of teeth,all the patients took 0.2 mg of buprenorphine sublingual tablet.The VAS score,Kuttner face score and side effects after dental treatment were recorded.Results:The VAS scores of the patients underwent preventive,operative and prosthetic treatments were 3.99?2.34,2.72?1.67 and 2.78?1.55,the Kuttner scores of those 1.98?1.23,2.66?1.04 and 2.03?0.93,respectively.Side effect was not observed in all the patients.Conclusion:Buprenorphine sublingual tablet can relieve the pain in dental treatment.

Arm Injuries ; etiology ; Autonomic Pathways ; pathology ; Central Nervous System ; pathology ; China ; Humans ; Occupational Diseases ; etiology ; Temperature ; Vibration ; adverse effects

Female ; Hand-Arm Vibration Syndrome ; diagnosis ; Humans ; Hypothermia ; diagnosis ; Male ; Reference Standards ; Skin Temperature ; physiology ; Young Adult

[Objective]To investigate the effects of alendronate(ALN)on subchondral bone and articular cartilage in the rat osteoarthritis model induced by anterior cruciate ligament transaction(ACLT).[Method]Twenty-four three-month-old female Sprague-Dawley rats were divided randomly into three groups:Sham group(n=8),ACLT+NS group(n=8) and ACLT+ALN group(n=8)(20 ?g?kg-1?2 d-1 subcutaneous injection for 12 weeks).Bone mineral density(BMD) measurement and bone histomorphometric analysis were made for the subchondral bone of right distal femur and proximal tibia in all rats at 12 weeks post surgery.Degree of cartilage degeneration was scored by Mankin scoring system.Immunostaining for matrix metalloproteinase 13(MMP-13) was performed to investigate the effect of ALN on cartilage matrix loss.[Result]BMD of the subchondral bone in the ACLT+NS group were significantly lower than those in the Sham group and the ACLT+ALN group(P

Objective To observe the changes of leptin levels in newly diagnosed type 1 diabetic patients and the effect of insulin therapy on serum leptin levels,so as to investigate the relationship between insulin and leptin.Methods Twenty-nine type 1 diabetic patients were enrolled from out-patient clinic and ward of our hospital.Twenty healthy people in our hospital were selected as normal controls.All blood samples were obtained after an overnight fasting for 14 hours.Fasting serum levels of glucose,hemoglobinA 1c(HbA 1c),triglycerides,cholesterol,insulin,C-peptide and leptin were analyzed in all subjects.The type 1 diabetic patients received insulin injection subcutaneously,and levels of glucose,insulin,C-peptide and leptin were measured 2 weeks later.Results In newly diagnosed type 1 diabetic patients,leptin levels were lower than those in normal controls (P0.05).It was found by correlation analysis that in control group,serum leptin levels were correlated with sex,BMI and serum insulin levels,while in newly diagnosed type 1 diabetic group,serum leptin levels were related to sex and serum insulin levels.Conclusion Serum leptin levels of patients with newly diagnosed type 1 diabetes are lower than those in control group.It is likely that short-term insulin injection subcutaneously can not affect serum leptin levels.