Objective To investigate the rural medical orientation undergraduate student's professional commitment,time management disposition and learning burnout and the relationship between them. Methods Use the college students' learning Burnout scale,adolescence time management disposition Questionnaire and College Students'professional commitment scale,survey of Xinxiang Medical University grade 2010 ~2012,739 valid ques?tionnaires,the questionnaire has 92. 61% efficiency. Results Three scores were normally distribution. Learning burnout was the medium level,the average score of each entry was 2.88±0.45,and moderate and above level learn?ing burnout ratio was 45.06%. The average score of time management disposition was 3.45±0.43. Professional com?mitment level was higher,the average score was 3.82±0.43 for each item. Affective commitment,ideal commitment, the sense of time efficacy and the sense of time control have entered on the learning burnout of the stepwise regres?sion equation,and four variables together explained 44.0% of the variance in the dependent variable. Time manage?ment disposition,the professional commitment and learning burnout were negatively correlated, but between time management disposition and professional commitment is positively related, professional commitment, the variance explained by variables of learning burnout was 30.12%. Conclusion The rural medical orientation of undergradu?ate students time management disposition are important factors of learning burnout,which has the forecast function to the learning burnout. Professional commitment is part of the intermediary variables.

Tumor suppressor CYLD is a deubiquitination enzyme that could regulate NF-?B and JNK signal pathways by deubiquitinating TRAFs,NEMO,Bcl-3 and p53,and in turn regulating the cell cycle and apoptosis.Loss or deficiency of CYLD would lead to the development of skin tumor,including multiple familial trichoepithelioma(MFT),familial cylindromatosis(FC),Brooke-Spiegler syndrome(BSS).Cancers like uterine cervix,kidney,and colon and hepatocellular carcinoma are related to the down-regulation of CYLD.

[Objective] To observe the cure effect of photodynamic therapy(PDT) combined with TCM on verruca acuminata. [Method] Select 80 cases of verruca acuminata, divide them into control group and treatment group, n=40 for each; the control one only took LE D-IB PDT therapeutic apparatus, the treatment one added with TCM on the base of control one; Observe their cure effects and side effects. [Result] The effective rate was 97.5% for treatment group, and 88.5% for control one, the treatment one was better than other one; there ’re 2 cases in each group respectively who had slight red and swol en skin of the il umination part, without side effects of erosion, ulcer, infection or scar;the recurrence rates were respectively 2.5% and 12.5% for both groups, the difference had statistical meaning. [Conclusion] PDT combined with TCM treating verruca acuminata had high cure rate, low recurrence rate, little side effects and high safety, without influencing patients ’life quality, with good compliance, worth clinical broad popularization.

The biodistribution and the radio activity in blood clearance of 99m Tc AMLCA were determined in 5 normal dogs by whole body imaging and measuring the radio activity in blood samples at 2,4,8 and 24h after 99m Tc AMLCA injection. The feasibility of imaging of the site of myocardial infarct was determined in 2 of the experimental MI dogs by demonstration of the left ventricle blood pool imaging (LVBPI) with 99m Tc AMLCA and by subsequent imaging of the excised heart. The results showed that the plasma concentration of 99m Tc AMLCA decreased rapidly from 51 5%?5 2% at 2nd hr to 27 3%?3 1% at 4th, 12 3%?1 8% at 8 hr and 5 6%?0 6% at 24th after the injection. The LVBPI in one MI dog showed that the region of the radionuclide concentration of 99m Tc AMLCA corresponded to region of absence of 99m Tc MIBI. The scintigraphy in another MI dog heart slices showed that the region of the radionuclide concentrate of 99m Tc AMLCA corresponded to the region of triphenyltetrazolium chloride (TTC) staining. The measurement of double radionuclides in the double interesting region in the MI dog heart slices indicated that the infarct myocardium uptook 99m Tc AMLCA specifically. These findings suggested that 99m Tc AMLCA scintigraphy might be a new approach for detecting and localizing MI

Alzheimer's disease (AD) is a common progressive degenerative disease of the central nervous system. It's also a main cause for dementia in presenium and senectitude. In the past few years, the discoveries in vitro expansion of neural stem cells (NSCs) indicate a new way for recovery and replacement of damaged neurons as well as reconstruction of the neural circuit, and offer a useful future therapy for AD. There are two main promising approaches in NSCs replacement therapy. They are endogenetic approach, inducing proliferation and differentiation of endogenetic NSCs, improving self-repair of central nervous system, and exogenous approach, ransplantation of exogenous tissue and promoting proliferation of endogenous NSCs. However, it may not become truth before the mechanism of NSCs' proliferation, migration, differentiation and it's integration with the host tissues to be elucidated.

Objective To evaluate the the safety and efficiency of precise liver resection for patients with primary liver cancer. Methods 86 patients with primary liver cancer were randomized to receive conventional routine hepatectomy (n=44) or the precise liver resection (n=42). Outcomes were compared between the precise hepatectomy group and the routine group, including, the blood loss, operation time, morbidity and mortality. Results There were significant differences in morbidity rates (7.1% vs. 20.5%; P<0.001), the blood loss [(320±315) ml vs. (613±526) ml;P<0.001) , postoperative alanine aminotransferase (ALT) value (in postoperation 7 d, 82.7 U/L vs.321.7 U/L; P<0.001) and length of hospital stay (12.3 d vs. 18.6 d; P<0.001) between precise hepatectomy and routine groups. The 1 year tumor recurrence rate and 1 year survival rate were 26.2%(11/42) and 78.6% (33/42) in precise liver resection group, 38.6% (17/44) and 65.9%(29/44) in routine liver resection group,with significant difference (P=0.010;P=0.018). Conclusion Precise liver resection is safe and effective in the treatment of liver tumor without much injury to patients.

Objective To investigate the effect of rosiglitazone on serum HMGB1,TNF-αand Th1/Th2 in patients with lung injury induced by sepsis.Methods 60 patients with sepsis lung injury in our hospital from August 2013 to August 2014, were randomly divided into experimental group and control group according to the medication methods, 30 cases in each group, control group was given conventional western medicine treatment, including intensive care, gastrointestinal decompression, fasting water, added blood volume, anti-inflammatory, maintenance of water and electrolyte balance, improve microcirculation, etc.On the basis of control group, experimental group was treated with rosiglitazone, two times per day, two weeks as a course of treatment.Serum HMGB1, TNF-α, Th1/Th2 were detected in 1, 3, 7, 14 days in all patients.ResuIts Compared with control group post-treatment,serum HMGB1,TNF-αand Th1/Th2 ratio were significantly decreased in the experimental group (P<0.05).ConcIusion Rosiglitazone can significantly reduce the levels of serum HMGB1,TNF-αand Th1/Th2.

Objective To establish a new method for rapid detection of electrolytes based on micro spectrometer and dry reagent.Methods The liquid electrolyte reagent was lyophilized for the preparation of reagentin powder form,which was then sealed into a detector cup of micro spectrometer for later experiment.During determination,the detector cup,in which the specimens and diluents were added to the dry reagent,was put into the detector slot of micro spectrometer,the contents in the detector cup were then well mixed by the magnetic stirring system of micro spectrometer and incubated for 6min with the heating system.Afterwards,the A values of potassium ion,sodium ion and chloride ion were detected respectively at 620nm,405nm and 456nm following the reaction principles of turbidimetry,enzymatic method and chemical method.Based on the findings of those detections mentioned above,the performance of the electrolytic dry reagent was evaluated,and the results were then statistically analyzed.Results The linear range of each parameter could meet the demand for clinical analysis,and the dry reagents had good reaction stability for 90 days after being lyophilized,with the intra-assay coefficient variation(CV) less than 4%,inter-assay CV less than 5%,and the recovery rate from 95% to 105%.No obvious interference was observed in the determination results under the circumstance that the serum TB was less than 290.40?mol/L and the TG was less than 11.20mmol/L.The detection results by this method were well correlated with that of dry chemical analysator VITRO S-250(R≥0.98).Conclusion The method is accurate and reliable in determining the serum electrolytes,and its process is simple process and convenient to carry out.Therefore,it can satisfy the condition for field aid as well as primary care.

Objective To explore the incidence and risk factor of renal artery stenosis in patients with coronary artery disease and essentiality of renal arteriography while performing coronary artery angiography. Methods Renal arteriography was performed immediately after coronary artery angiography in 114 patients with suspected coronary artery disease. Results Incidence of renal artery stenosis was 18.4% (21/114) in 114 patients and 26% (20/77) in patients with coronary artery disease who were identified by coronary artery angiography. Only one case with renal artery stenosis was found in 37 cases whose coronary arteries were normal (2.7%, 1/37). Incidence of renal artery stenosis in patients with coronary artery disease was higher than that in patients with normal coronary artery (26% vs 2.7%, P

Objective To investigate the properties of cultured periosteal cells associated with bone morphogenetic protein(BMP) to find a new treatment for bone defects.Methods The periosteal cells were cultured,expanded and passage cultured in vitro to investigate the characteristics and functional changes of rabbit periosteal cells.Animal models of 1.0cm gaps with complete removal of the bilateral radius medium bone and periosteum were induced in 24 New Zealand rabbits,which were randomly divided into 4 groups to receive the defect repair with periosteal cells combined with BMP,periosteal cells,BMP and blank control respectively.X-ray and histological examination were made periodically after operation to evaluate the effectiveness of bone formation.Results Periosteal cell morphology can be divided into three stages: cell division phase,cell growth phase and cell functional phase.Cellular configuration had no significant difference with primary cells after anagenesis.The callus can be formed after the periosteal cells were transplanted into the bone defection.The osteogenesis ability of periosteal cells combined with BMP was better than that of the BMP,and the osteogenesis ability of the BMP was better than that of the periosteal cells.The mechanism of bone regeneration for periosteal cells was similar to intramembranous ossification,and that of BMP was similar to endochondral ossification.In the periosteal cells combined with BMP group,both intramembranous and endochondral ossification existed,and the former was obvious.Conclusion Both periosteal cells and BMP can improve the repair of bone defect in vivo.Cultured periosteal cells combined with BMP had substantial ability of synergistic osteogenesis,and it was an ideal treatment for bone defection.