1.ABCE1 gene silencing effect on biological characteristics of human cervical cancer cells XB1702
Chinese Journal of Tissue Engineering Research 2015;(37):5972-5977
BACKGROUND:With the development of genetic engineering and tumor molecular biology, gene therapy has acted as a new treatment mode for tumors. OBJECTIVE:To investigate the impact of gene silencing ABCE1 on growth, proliferation and migration of human cervical cancer cel s XB1702. METHODS:siRNA sequences of ABCE1 were designed and synthesized to transfect XB1702 cel s using LipofectamineTM 2000. Cel s transfected with NC siRNA carrier served as controls, and untransfected cel s acted as blank group. After RNA interference, the expression of ABCE1 mRNA and protein was detected by western blod assay;cel cycle was detected using flow cytometry;cel counting kit-8 test, scratch healing assay and cel invasion assay were used to evaluate the effects of ABCE1 silence gene on XB1702 cel proliferation, migration and invasion ability. RESULTS AND CONCLUSION:ABCE1 mRNA and protein expression in the experimental group was significantly lower than that in the control group and blank group (P<0.05). Growth rate in the experimental group was significantly slowed, cel s were arrested in G 0/G 1 phase, and the number of S-phase cel s was reduced. Compared with the control group and blank group, XB1702 cel proliferation was inhibited significantly in the experimental group, and cel migration and invasion was significantly decreased (P<0.05). These results showed that specific interference with gene expression of ABCE1 can inhibit the migration of human cervical cancer cel s XB1702, and restrain tumor cel proliferation;therefore, the siRNA sequence of ABCE1 may be an effective target for treatment of cervical cancer.
2.Determination of three anti-tuberculosis medicines in focus of patients with tuberculosis of the wrist and its clinical significance
Journal of Chinese Physician 2009;11(3):296-298
Objective To study the distribution of rifampicin,isoniazid in tissues of patients with tuberculosis wrist for the sake of providing siven guide toward operation and postoperative chemotherapy.Methods 32 patients with tuberculosis wrist,according to radiographic data,were divided into Center group(group Ⅰ)and Borderline groups(group Ⅱ).All patients received chemotherapy regimen of 3SHR(T)Z/6HR(T).After treatment for4 weeks,the patients'specimen of serum,and tuberculosis tissues(group Ⅰ)including sclerotic bone wall,sub-normal bone and tuberculosis tissues(group Ⅱ)including dissolved bone wall,sub-normal bone were obtained during operation at 1,2 hours respectively after oral medication.Then pretreatment and mensuration using HPLC method were carried out after specimen's removal.Results Concentrations of isoniazid and rifapentine in(group Ⅰ)and(group Ⅱ)sub-normal bone and dissolved bone were abeve the minimal bactericidal concentration values.Concentration of 2 drugs in(group Ⅰ)sclerotic bone wall were much lower than that in sub-normal bone and dissolved bone wall.Conclusion The concentration of 2 drugs in patients sclerotic bone wall of wrist were too lower to eliminate the mycobacterium tuberculosis,while an effective concentration for anti-tuberculosis chemotherapy should be find in sub-normal bone and dissolved bone of wrist.Thus,the sclerotic bone of wrist plays an important role in obstructing the drags penetration into tuberculosis.
3.The values of combined detection of C-reactive protein,white blood cell count and neutrophil ratio in infection
International Journal of Laboratory Medicine 2014;(19):2607-2608
Objective To evaluate the values of combined detection of C-reactive protein ,white blood cell count and neutrophil ratio in infection .Methods 158 patients diagnosed with infection were divided into bacterial infection group and non-bacterial infec-tion group .The concentrations of C-reactive protein ,white blood cell count and neutrophil ratio were detected ,which were compared with cases in the control group .Results The concentrations and abnormal rates of C-reactive protein ,white blood cell count ,neu-trophil ratio in bacterial infection group were significantly higher than those in non-bacterial infection group and control group(P<0 .01) .The concentration and abnormal rate of C-reactive in non-bacterial infection group were significantly higher than those in control group(P<0 .05) .Conclusion Determination of C-reactive protein can supplement the lack of white blood cell count and neutrophil ratio ,determine the degree of inflammation or injury .The combined detection of C-reactive protein ,white blood cell count and neutrophil ratio in infection has important clinical significance .
4.Effects of aerobic exercise on 6-minute walk distance and serum level of omentin-1 in hemodialysis patients
Tianjin Medical Journal 2016;44(8):1014-1017
Objective To explore the effects of aerobic exercise on 6-minute walk distance and serum level of omentin-1 in maintenance hemodialysis (MHD) patients. Methods A total of 89 MHD patients (MHD group) in the 5th Central Hospital of Tianjin were included in the study from December 2013 to November 2014. Patients were randomly divided into two groups:routine treatment group (n=43) and aerobic exercise treatment group (aerobic exercise group, n=46). Fifty healthy subjects were selected as normal control group. Both groups received the same hemodialysis and regular treatments, and the aerobic exercise group received 6-month interdialytic aerobic exercise, and the routine group did not take any exercise intervention. The 6-minute walk distances (6MWDs),body mass,height and blood pressure were recorded, and serum levels of omentin-1, C-reactive protein (CRP), blood routine test and biochemistry test were determined in two groups. The relationship between 6 MWDs, age, CRP, left ventricular ejection fraction (LVEF) and omentin-1 were analyzed. Results The serum levels of omentin-1 and CRP weresignificantly higher, LVEF was significantly lower, in MHD group than those of healthy control group (P<0.01). After 6 months of aerobic exercise, 6MWDs, LVEF, hemoglobin and serum level of omentin-1 were significantly increased than those before treatment in aerobic exercise group. The 6MWDs, LVEF and hemoglobin were significantly increased after treatment than routine group. The levels of CRP, fasting glucose and blood pressure were significant decreased after treatment. The levels of CRP, fasting glucose and systolic blood pressure were decreased in treatment group than those of routine group. Before treatment, 6MWDs was negatively related with age and CRP, and positively related with LVEF and serum level of omentin-1 in MHD patients (r=-0.418,-0.229, 0.252 and 0.234, P<0.05). Conclusion Aerobic exercise can significantly increase values of 6MWDs, LVEF, hemoglobin and serum level of omentin-1, and reduce values of CRP, fasting blood glucose and systolic blood pressure in MHD patients. Aerobic exercise can improve physical performance,heart function and micro inflammatory state, thereby reduce cardiovascular events and mortality risk in MHD patients.
5.Research on the development and clinical application of soak pager device
China Medical Equipment 2015;(12):55-57
Objective:To evaluate the clinical effect of the independent research and development of the soak paper device used for patients in bed.Methods: To add a soak sensitive receiver and a soak signal transmitter on the extension pager device in the ward and have them connected with the wire to form a new type of soak pager device.Results:Using a new type of wet pager device can transmit signal in time when one-time pad single was wet for the patients in bed and avoid skin wet of the patients for a long time. All the patient and their families were very satisfied.Conclusion: The self-made and a new type of wet pager device used for patients in bed can better protect the patient’s skin and save manpower. The device is convenient and simple, the application is safe and effective.
8. Effects of ischemic postconditioning and controlled low-pressure reperfusion on ischemia and reperfusion-induced spinal cord injury in rats
Academic Journal of Second Military Medical University 2010;29(11):1341-1346
Objective: To investigate the effects of ischemic postconditioning and controlled low-pressure reperfusion on ischemia and reperfusion-induced spinal cord injury. Methods: Spinal cord ischemia was induced by occlusion of the descending thoracic aorta combined with maintaining systemic hypotension (40 mmHg,1 mmHg=0.133 kPa) in rats. Rats were randomly divided into three groups (n = 103), namely, control group, postconditioning group (Post-con group) and low-pressure reperfusion group (LR group). Rats in control group underwent an abrupt increase to 100 mmHg in mean arterial pressure (MAP) after reperfusion. Those in the Post-con group underwent 3 cycles of 30-second reperfusion and 30-second occlusion after 30-second full reperfusion with MAP of 100 mmHg. The MAP maintained 40 mmHg for the initial 3 min of reperfusion in the animals of the LR group, then increased to 100 mmHg. The relative spinal cord blood flow (rSCBF) was monitored simultaneously during the ischemia and 30 min after reperfusion. The activities of superoxide dismutase (SOD),catalase (CAT) and myeloperoxidase (MPO) and malondialdehyde (MDA) content in lumbar spinal cord tissue were measured at 1,4,12 and 24 h after reperfusion,and the neurologic function of hind-limb was also evaluated at the same time. The samples of lumbar spinal cord were collected in all groups at the end of observation for histological examination. Results: Postconditioning and low-pressure reperfusion both improved the hyperperfusion after reperfusion. In Post-con group the activities of SOD and CAT was markedly increased 1 and 4 h after reperfusion (P<0.01) compared with control group, and no marked increase in MDA and MPO was observed. The MDA in the spinal tissue of LR group was significantly lower than that of the control group (P< 0.01), but no significant increases were observed in the activities of SOD and CAT. The MPO activity in LR group peaked from the 1st to 4th hour of reperfusion and then gradually reduced. Postconditioning and low-pressure reperfusion both improved the neurologic function during reperfusion, but behavioral scores of animals in LR group were lower than that in the Post-con group. The pathological results had a similar pattern to the behavioral scores in all 3 groups 24 h after reperfusion. Conclusion: Ischemic postconditioning and controlled low-pressure reperfusion can both attenuate ischemia and reperfusion injury in spinal cord, and the neuroprotection of ischemic postconditioning is superior to controlled low-pressure reperfusion, probably because postconditioning can enhance activities of antioxidant enzymes and decrease neutrophil accumulation.
9.Analysis and Detection of IgM to Mycoplasma pneumoniae in 225 Children
Li-Ping WANG ; Qi LV ;
Chinese Journal of Nosocomiology 2009;0(24):-
OBJECTIVE To estimate the value of Mycoplasma pneumoniae(Mp)-IgM,C-reactive protein(CRP) and WBC on diagnosis of children respiratory infection.METHODS Serum Mp-IgM antibody,CRP and WBC were tested in 225 children with respiratory infection by passive particle agglutination,ITM immunoturbidimetry and analyzer of blood cell.RESULTS Forty-six cases of 225 children were identified positive in Mp-IgM.The positive rate of Mp-IgM was 20.4%.Compared the CRP and WBC among cases positive in Mp-IgM,bacterial infected and virus infected,there was significant difference bewteen cases positive in Mp-IgM and bacterial infected,no significant difference bewteen cases positive in Mp-IgM and virus infected.CONCLUSIONS The joint detection of serum Mp-IgM antibody,CRP and WBC can be a valuable clue to diagnosis of children respiratory infection.
10.Job demand survey of vocational medical laboratory technology in Jiangsu Province
International Journal of Laboratory Medicine 2015;(14):2065-2067
Objective To study the job demand of vocational medical laboratory technology in Jiangsu Province ,and as a basis to build curriculum system of vocational medical laboratory technology .Methods We surveyed 128 medical laboratory related institu‐tions in Jiangsu Province ,the survey included the demand for talent ,changing needs of reasons ,demand for vocational talent and job skill requirements ,data of the survey results were collected and analyzed .Results Demand for vocational talents for tertiary hospi‐tals were small ,while the primary health care institutions and relevant private medical institutions and companies were exuberant . The main jobs were clinical testing technology direction and pathological examination technical direction .In addition ,blood transfu‐sion and transfusion technology direction ,testing reagents and equipment marketing direction and technical for andrology laboratory direction also were needed .Conclusion According to the survey results ,we determine educational objectives ,basic requirement and specialty orientation of vocational medical examination major ,and propose clinical laboratory specializing in the basic platform+specialty orientation modulefor the curriculum system of vocational medical examination major .