Objective For the purpose of exploring the influence exerued by the amount of template DNA on the results of automatic fluerescent detection. Method For the purpose of buiding the optimal amount of template DNA amplified, we have analyzed different amount of template DNA (9947A) by 3100 Genetic Analyzer with the multiplexing Profiler Plus Kit. Results The optimal amounts of template DNA amplified were between 0.31ng and 2. 5ng by using 3100 Genetic Analyzer. Conclusion It will produce the incorrect analytic results if the amounts of template DNA were too high or too low.

Objective To observe the clinical curative effect of Xiaoer Chiqiao Qingre granule in the treatment of infantile upper respiratory tract infections.Methods 100 children with upper respiratory tract infection were selected,and they were randomly divided into observation group and control group,50 cases in each group.The observation group was treated with Xiaoer Chiqiao Qingre granule,the control group was given ribavirin effervescent granules.The effects of the two groups were observed.Results The total effective rate of the observation group was 96％,which was significantly higher than 78％ of the control group (x2 =9.500 6,P ＜ 0.05).The fever,symptoms and signs of improvement time in the observation group were better than those in the control group.Conclusion Xiaoer Chiqiao Qingre granule in the treatment of infantile upper respiratory tract infection can defervesce quickly,and has quick curative effect,so it is worthy of promotion.

Objective To explore the therapy measurement of iron deficiency anemia(IDA)associated helicobacter pylori(HP)infection among children.Methods Eight hundred and ninty nine cases entrusted children aged from 2 to 7 years old were measured hemoglobin(Hb),mean corpuscular volume(MCV)mean corpuscular hemoglobin(MCH),mean corpuscular hemoglobin concentration(MCHC)and serum ferri,serum fettin(SF),HP-IgG antibodies.Divided the HP-IgG positive cases into two groups for treatment,The treatment group adopted omeprazole,amoxicillin and metronidazole to cure them for two weeks,then oral ferrous sulfate sugar liquid.The contrast group purely take the ferrous sulfate sugar liquid.After eight weeks re-examined the Hb and SF.Results Totally 279 cases were diagnosed as Hp-positive,620 as HP-negative and 99 children diagnosed as IDA,among the 99 cases IDA,58 cases with HP infection(20.8%) and 41 cases with non-HP infection(6.7%).That means the number of IDA cases with HP infection was higher than those with non-HP infection(?2=39.46 P

Objective To explore the effect of androgen on learning and memory ability and neurons in hippocampal CA1 region in senescence accelerated mouse prone strain/8(SAMP8).Methods Thirty 7-month-old male SAMP8 were randomly divided into sham-operation control group,castrated group and androgen replacement therapy after castration group.The dose of testosterone undecanoate(TU) was 37.4mg/(kg?15d).The capability of learning and memory was observed 45 days later through the Morris water maze(MWM) test and the change of neurons in hippocampal CA1 region was detected and analyzed by HE staining,immunohistochemal method and computer pathological image analysis system.Results 1.In the MWM test,the escape latency of castrated group were significantly prolonged(P0.05).2.With HE staining,neurons in hippocampal CA1 region of castrated group were found with diffused vacuolar degeneration,and sparse and disordered cellular arranpement.The cell nucleuses were karyochrome and karyopycnosis.The number and optical density of A? immune positive neurons were markedly higher than those of other groups(P

Female ; Humans ; Laryngeal Diseases ; surgery ; Laser Therapy ; Lasers, Solid-State ; Polyps ; surgery ; Sarcoidosis ; surgery ; Vocal Cords

Objective To analyze the differences in the clinical characteristics,endoscopy,pathology and therapy between the patients with new onset ulcerative colitis(UC) in the elderly versus youth and middle-aged patients.Methods A review analysis was carried out in the 178 hospitalized patients with UC in Third Hospital of Peking University from 1994 to 2010.The patients were divided into two groups according to the age of onset:UC onset at age of 60 years and older were enrolled in elderly group; UC onset at age less than sixty years were enrolled in youth and middle-aged group.The data of clinical manifestation,endoscopy,pathology,laboratory test,the severity of disease classification and therapy were analyzed and compared between the two groups.Results The elderly group consisted of 21 men and 6 women.The youth and middle-aged group consisted of 83 men and 68 women.The ratio of male was higher in elderly group than in youth and middle-aged group (77.8％ vs.55.0％,P＜0.05).No significant difference in the clinical type of UC was observed (P＞0.05).The ratio of abdominal pain in elderly group was lower than in youth and middle-aged group (44.4％ vs.78.8％,P＜0.05).There were no significant differences in other symptoms,laboratory test and the severity of disease between the two groups (all P＞0.05).The ratio of Grade Ⅰ and Ⅱwas much higher in the elderly than in youth-middle-aged group(70.4％ vs.39.9％,P＜0.05),but the ratio of Grade Ⅲ and Ⅳ was much lower in the elderly than in youth-middle group(29.6％ vs.60.1％,P＜0.05).No significant differences between the two groups were found in the extent of disease,pathological characteristics and therapy (all P＞0.05).Conclusions Compared with the youth and middle-aged patients with onset UC,the ratio of male patients was higher,the ratio of abdominal pain was lower,and the severity of endoscopic manifestation was less in the elderly patients with onset UC.

Alveolar Epithelial Cells ; cytology ; metabolism ; Animals ; Apoptosis ; Lung ; drug effects ; metabolism ; Nitric Oxide ; pharmacology ; Rats ; Rats, Sprague-Dawley ; Tyrosine ; analogs & derivatives ; metabolism

Objective:To investigate the effect of glucocorticoid on treating class IV lupus nephritis(LN) by comparing the content of serum transforming growth factor beta(TGF ? 1).Methods:The study consisted of 33 objects,15 health controls as well as 18 cases of IV LN.The level of serum TGF ? 1 was analyzed by ELISA.Results:The level of serum TGF ? 1 was greatly reduced after the patients were treated with glucorcorticoid.Conclusion:Timely and sufficient glucocorticoid may prevent IV LN from developing into glomerulosclerosis.

AIM: To assess the impact of diabetes on corneal endothelial cells through the quantitative analysis of corneal endothelial cell morphology for patients with diabetics.
METHODS: The corneal thickness and endothelial cell morphology of 360 eyes of 299 cases were detected using full automatic corneal endothelial cell analyzer. The normal control group included 175 eyes of 148 cases, and there were 185 eyes of 151 cases for the patients with diabetes, 110 eyes of 92 cases for the non-proliferating phase group and 75 eyes of 59 cases for the proliferating phase group. The average density of central corneal endothelial cells, proportion of hexagonal cells, coefficient of variation and corneal thickness were compared among groups, and then the statistical analysis was conducted.
RESULTS: Compared with the cornea of the normal group, in the diabetes group, the coefficient of variation of corneal endothelial cells and central corneal thickness increased, while the average density of central corneal endothelial cells and proportion of hexagonal cells decreased, showing a significant difference (P<0. 05). Compared with the cornea of non-proliferating phase group, in the proliferating phase group, the density of central corneal endothelial cells decreased, the coefficient of variation of corneal endothelial cells increased, while the proportion of hexagonal cells decreased with a significant difference (P<0. 05), and the central corneal thickness increased, showing no significant difference(P>0. 05).
CONCLUSION: Compared with the cornea of normal control group, in the diabetes group, the corneal endothelial cells show abnormal morphology, which aggravates with the severity of lesions, especially for the significant changes in the coefficient of variation and the proportion of hexagonal cells. As a result, the corneal resistance to damage in patients with diabetes will decrease.