Humans ; Laryngeal Neoplasms ; pathology ; Male ; Middle Aged ; Neurilemmoma ; pathology

The purpose of this paper is to achieve a measurement of temperature prediction for artificial heart without sensor, for which the research briefly describes the application of back propagation neural network as well as the optimized, by genetic algorithm, BP network. Owing to the limit of environment after the artificial heart implanted, detectable parameters out of body are taken advantage of to predict the working temperature of the pump. Lastly, contrast is made to demonstrate the prediction result between BP neural network and genetically optimized BP network, by which indicates that the probability is 1.84% with the margin of error more than 1%.
Heart, Artificial ; Neural Networks (Computer) ; Temperature

ObjectiveTo study the relationship between the activity of daily living (ADL) and the physiopsychic variables of the patients at early recovery stage after first stroke. Methods ADL and the motion activities, the deficient degree of nerve function, the cognitive function and the depressive degree of the 48 patients at early recovery stage after first stroke were evaluated. ResultsThere are significant relationship between ADL at early recovery stag and the motion activities, the deficient degree of nerve function, the cognitive function of the patients at acute period(P<0.01). Meanwhile, there are also relationship between ADL and the number of the cerebral lesion, the hemiplegic degree and the education degree of the patients (P<0.05) at early recovery stage.Conclusions The motion activities, the deficient degree of nerve function and the cognitive function of the stroke patients at acute stage are the important predictive factors to ADL of the patients at early recovery stage, and the number of the cerebral lesion, the hemiplegic degree and the educational level of the patients maybe influence ADL of the patients at the early recovery stage.

OBJECTIVETo explore the effect and possible cell signal transduction mechanism of active components (alkaloids and glucosides) of Buyang Huanwu Decoction (BHD) on the vascular adhesion molecule (VAM) expression induced by thrombin (Thr).

METHODS(1) Endothelial cells (ECV-304) were cultured in vitro and stimulated by Thr, then cultured in media containing BHD whole recipe (BHDw), its alkaloids (BHDa) or glycosides (BHDg), respectively. The expressions of E-selectin, CD31, CD54, and protein Kinase Calpha PKCa) were detected by immunocytochemical method. (2) Cultured ECV-304 cells were treated with Thr, Thr + 1-(5-isoquinolinesulfonyl)-2-mythylpiperazine dihydrochloride (H7), phorbol myristate acetate (PMA), PMA + H7, Thr + cathepsin-6 (CATG) as well as Thr plus BHDw, BHDa, and BHDg, respectively after then, changes of PKCalpha, phospho-p38 mitogen-activated protein kinase (p-p38MAPK), phospho-extracellular signal regulated kinase (p-ERK) and nuclear factor-kappaB (NF-kappaB) expressions were observed.

RESULTS(1) Expressions of PECAM-1, ICAM-1 and E-selectin in ECV-304 cells were significantly enhanced after thrombin stimulation (P < 0.05). When compared with the thrombin group, the three expressions were lower in the BHDw treated group (P < 0.05); while in groups treated with BHDa and BHDg, only expressions of PECAM-1 and ICAM-1 were lower (P < 0.05), but no obvious difference in E-selectin was shown. (2) Expression of endothelial PKCalpha increased after thrombin stimulation (P < 0.01), which could be enhanced by PMA (PKC activator, P < 0.01); but inhibited by PMA + H7 (PKC inhibitor), CATG (PAR-1 inhibitor, P < 0.05) as well as by BHDw, BHDa and BHDg (P < 0.05). (3) Expressions of p-p38 MAPK and p-ERK in ECV-304 cells showed no remarkable change after thrombin stimulation, or after reacted with PMA, H7, CATG, BHDw, BHDa and BHDg. (4) Similar to that of p-p38MAPK and p-ERK, NF-kappaB was unchanged in all the reactions.

CONCLUSIONSBHD could down-regulate the increasing of VAM expression induced by thrombin in VECs; BHDa and BHDg might be the active components in the recipe. The effect of thrombin is mainly mediated through activation of PKC; while p-p38MAPK, ERK or NF-kappaB are not the chief signal transduction pathways. And BHD and its effective components may antagonize the thrombin activation on VECs through inhibiting the activation of PKCalpha.

Cell Adhesion Molecules ; metabolism ; Cell Line ; Drugs, Chinese Herbal ; pharmacology ; E-Selectin ; metabolism ; Endothelial Cells ; drug effects ; metabolism ; Extracellular Signal-Regulated MAP Kinases ; metabolism ; Humans ; Intercellular Adhesion Molecule-1 ; metabolism ; NF-kappa B ; metabolism ; Platelet Endothelial Cell Adhesion Molecule-1 ; metabolism ; Protein Kinase C-alpha ; metabolism ; Signal Transduction ; Thrombin ; pharmacology ; p38 Mitogen-Activated Protein Kinases ; metabolism

Metabolomics is a new science and technology, which it refers to a holistic analytical approach to all the low molecular weight metabolites in an organism or a cell. In this paper, the definition and objective of metabolomics are provided, and the current application of metabolomic research in malignant tumors (diagnosis and therapy) are summarized.
Animals ; Biomarkers, Tumor ; genetics ; metabolism ; Genomics ; methods ; trends ; Humans ; Metabolism ; Models, Biological ; Neoplasms ; genetics ; metabolism ; Proteomics ; methods ; trends ; Transcription, Genetic

OBJECTIVETo investigate the periodic quantity variation of proliferating neuronal progenitors after global brain ischemia and provide evidence for choosing the time-window of drug therapy to promote neuronal regeneration after ischemia.

METHODSAdult male Wistar rats were subjected to 15-min global brain ischemia (four-vessel occlusion model) and randomized subsequently into 8 groups (n=3). The rats were given intraperitoneal injections of BrdU (75 mg/kg) for 4 times daily (at a 2-hour interval) since day 7 till day 11 after ischemia, and on day 29, the rats were perfused transcardially for fixation. Another 3 normal rats were given BrdU in the same manner and killed the next day. Coronal sections of the brain tissue (30 microm) were prepared for immunocytochemical detection of BrdU-labeled cells and immunofluorescent detection of BrdU/NeuN double-labeled cells. The density of BrdU-positive cells and BrdU/NeuN double-labeled cells in the hippocampal dentate gyrus (DG) and CA1 region were counted and the density of proliferating cells at different days after ischemia were compared using one-way ANOVA.

RESULTSThe proliferation of the neuronal progenitors increased after global brain ischemia. The number of BrdU-positive cells in the DG and CA1 region decreased gradually in 7-10 days after ischemia, and reached the normal level during 11-14 days. The differentiation of the progenitors did not vary after ischemia.

CONCLUSIONIncreased proliferation of the neuroprogenitors occurs mainly within the initial 10 days after global ischemia in rats.

Animals ; Brain Ischemia ; pathology ; physiopathology ; Cell Differentiation ; Cell Proliferation ; Male ; Nerve Regeneration ; Neurons ; pathology ; Random Allocation ; Rats ; Rats, Wistar ; Stem Cells ; pathology ; Time Factors

OBJECTIVETo provide evidence for intervention measures on safe injection through a survey on the safety of injection in the health facilities in Wulong county, Chongqing city, China.

METHODSAn investigation on injection safety was conducted among health care providers with a uniform questionnaire.

RESULTSGlass syringes which were not appropriately sterilized was found in 52.2% (12/23) of the health facilities. Injection practices were not correct in 31.4% (11/35) of the health facilities. Used disposable syringes were discarded directly without any disposal in 63.6% (21/33) of the health facilities. At least one of the unsafe injection practices mentioned above existed in 77.1% (27/35) of the health facilities.

CONCLUSIONSThe main unsafe factors in the practice of injection are included: the practice to change needle but not syringe in penicillin allergic test and the injuries caused by used disposable syringes in medical staff. The important measures to improve injection safety were to train the medical staff on the knowledge about safe injection and the risks caused by unsafe injection, and to equip the health facilities with safe tools to destroy the used disposable syringes.

Adult ; China ; epidemiology ; Cross-Sectional Studies ; Disinfection ; statistics & numerical data ; Disposable Equipment ; statistics & numerical data ; Equipment Reuse ; statistics & numerical data ; Female ; Health Knowledge, Attitudes, Practice ; Humans ; Injections ; adverse effects ; statistics & numerical data ; Male ; Risk Factors ; Rural Health Services ; Safety ; Sampling Studies ; Surveys and Questionnaires ; Syringes

OBJECTIVETo explore a way of determining the weights of variables in synthetic evaluation.

METHODSBased on the attribute synthetic evaluation system, similarity weight was used to cluster and rank the atmospheric quality on 11 large cities.

RESULTSRealized the classification and ranking for the atmospheric quality of the cities given, the results were almost comparable to that achieved by Topsis evaluation.

CONCLUSIONThe method of comprehensive assessment using similarity weight in the attribute synthetic evaluation system seemed to be objective and rational. Not only it embodied the weights of variables involved, but also exploiting the information presented by the sample.

Air Pollution ; prevention & control ; statistics & numerical data ; China ; epidemiology ; Data Interpretation, Statistical ; Decision Making ; Environmental Monitoring ; Epidemiological Monitoring ; Evaluation Studies as Topic ; Humans ; Models, Theoretical

Objective To discuss the application of nano-hydroapatic crystals and polyamide composite(n-HA/PA66) for artificial vertebrae in treating thoracic or lumbar vertebrae tumor.Methods A total of 14 cases suffering from thoracic or lumbar vertebrae tumor underwent deuto-total vertebrae resection via anterior approach,then canalis vertebralis was deboosted,artificial vertebrae was implanted and fixed with screw-stick system.Fourteen patients,9 male and 5 female were admitted by our hospital from Dec 2003 to Sep 2005,aged 32 to 68 years old(average 50).The tumor located at T_(11) in 2 cases,T_(12) in 2,L_1 in 5,L_2 in 5.Pathological diagnosis showed 3 cases were of aneurysmal bone cyst,5 of hemangioma,2 of giant cell tumor,3 of metastatic thoracic lumbar vertebra tumor. Results The follow-up lasted 3-18 months,mean 10 months.The rate of bone graft fusion reached 85.7%,with no loosened internal fixation or breakage of screw or stick.No screw breakage or spinal instability occurred during the follow-up.All cases were alive till follow-up finished.Conclusion If the thoracic vertebral tumor compresses spinal cord seriously,the patients should be recommended for surgical treatment.Total vertebrectomy followed by reconstruction could relieve the compression caused by tumor and restore the stabilization of spine effectively.Artificial vertebrae is the ideal substitute material for bone transplantation,for it has good biocompatibility,high security,high rate of bone graft fusion and firm fixation.

Objective To evaluate the relationship between single nucleotide polymorphism(SNP) of candidate genes and radiation-induced esophagitis (RIE) in patients with lung cancer. Methods Between Jan. 2004 and Aug. 2006,170 patients with pathologically diagnosed lung cancer were enrolled in this study. The total target dose was 45-70 Gy( median 60 Gy). One hundred and thirty-two patients were treated with three-dimensional conformal radiotherapy(3DCRT) and 38 with two-dimensional radiotherapy(2DRT).Forty-one patients received radiotherapy alone, 78 received sequential chemoradiotherapy and 51 received concurrent chemoradiotherapy. Thirty-seven SNPs in 20 DNA repair genes were analyzed by using PCR-based restrieted fragment length polymorphism(RFLP). These genes were apoptosis and inflammatory cytoking genes including ATM, ERCC1, XRCC3, XRCC1, XPD, XPC, XPG, NBS1, STK15, ZNF350, ADPRT,TP53, FAS, FASL, CYP2D6 * 4, CASPASE8, COX2,TGF-β, CD14 and ACE. The endpoint was grade ≥2 R I E. Results Forty of the 170 patients developed grade ≥2 R I E, including 36 in grade 2 and 4 in grade 3. Univariate analysis revealed that radiation technique and concurrent chemoradiotherapy were statistically significant relatives to the incidence of R I E (P = 0. 032,0.049) , and both of them had the trend associating with the esophagitis( P = 0.072,0. 094 ). An increased incidence of esophagitis was observed associating with the TGF-β1-509T and XPD 751 Lys/Lys genotypes ( χ2 = 5.65, P = 0.017 ;χ2 = 3.84, P = 0. 048 )in multivariate analysis. Conclusions Genetic polymorphisms in TGF-β1 gene and XPD gene have a significant association with radiation-induced esophagitis.