BACKGROUND:Heart transplantation is an effective method for treatment of end-stage heart failure, but immune rejection that seriously impact therapeutic effacicy is easy to occur after transplantation. OBJECTIVE:To investigate the regulatory effect of bone marrow mesenchymal stem cel s on the immune function of rats undergoiong heart transplantation. METHODS:Twenty Lewis rats were enrol ed as donors, and 20 Wistar rats as recipients. Heart transplantation models were established in the Wistar rats. These 20 model rats were randomized into cel transplantation and control group with 10 rats in each group. Forty-eight hours after heart transplantation, rats in the cel transplantation group were given bone marrow mesenchymal stem cel suspension (1 mL, 2×108 cel s/L) via the tail vein, while rats in the control group were given normal saline in the same dose. Then, the expression levels of serum interleukin-2, interleukin-10 and percentage of CD4+, CD8+, CD4+/CD8+, CD4+CD25high, CD4+CD25high Foxp3+T cel s in the venous blood were detected in the two groups at 7 days after cel transplantation. Additional y, rat myocardial tissues were taken and observed pathological y. RESULTS AND CONCLUSION:The survival time of the cel transplantation group was significantly longer than that of the control group (P<0.05). The expression level of interleukin-2 showed no significant difference between the two groups (P>0.05), but the level of interleukin-10 in the cel transplantation group was significantly higher than that in the control group (P<0.05). Compared with the control group, the percentage of CD4+/CD8+, CD4+CD25high, CD4+CD25high Foxp3+and CD4+T cel s was significantly higher, and the percentage of CD8+T cel s was significantly lower in the cel transplantation group (P<0.05). Histopathological findings showed that there were a smal amount of infiltrated lymphocytes in the cel transplantation group with the presence of slight bleeding and edema, and these inflammatory reactions were milder than those in the control group. These findings indicate that bone marrow mesenchymal stem cel transplantation can effectively reduce the rejection in rats undergoing heart transplantation.

Social justice,as a part of the public policy and legal value,is an important target of the rule of law and should penetrate into all the activities in medical field.The doctor-patient relationship influences the realization of medical justice directly.This article analyses the status of the missing integrity between doctors and patients and the medical hazards it brings,from the perspective of the practice of doctor-patient relationship,and put forward the methods to construct the integrity of medical treatment.

Objective To investigate the turnover and factors influencing amniotic membrane transplantation into corneal stroma.Methods Amniotic membrane stained with dichlorotriazinyl aminofluoresclin (DTAF)was implanted into the corneal stroma of 1 5 New Zealand white rabbits.After transplantation,we observed the clinical changes.The tissue samples from grafted area were observed with HE staining and laser confocal microscope to trace amniotic membrane at 4,8,13,1 7 and 22 week after surgery.And the tissue samples at week 22 after surgery from grafted area were also observed by transmission electron microscopy.Results After 4 weeks of operation, amniotic membrane tissue bent with visible multi-layer homogeneous red dye.The structure remained intact;basement membrane and stroma were no dissolved.After 8 weeks, amniotic tissue was a strip structure of homogeneous red dye,and the substance layer was complete.After 13 weeks,amniotic membrane morphology appeared fuzzy,but still took the shape of strips;a small number of corneal cells accumulated around the amniotic membrane.At 1 7 weeks after operation,fragmented amnion was evident.At 22 weeks after operation,small pieces of amnion tissue remained in the corneal stroma.Laser scanning confocal microscopy revealed that amniotic membrane could retain for over 22 weeks in the corneal stroma.After 22 weeks,amnion stroma collagen fibers that were integrated into the corneal stroma were irregular.Corneal stromal ultrastructure surrounding the amniotic membrane did not obviously change.Conclusion Retention time of amniotic membrane transplanted into corneal stroma can be as long as over 22 weeks and amniotic membrane can be merged into the corneal tissue as a substitute, which wins time for corneal transplantation operation.

Humans ; Salivary Glands

Immobilization is a usual method to treat the injury of skeletal muscle system and plays an important role in rehabilitation of diseases. But immobilization also displays some negative effects, of all, muscle system is affected significantly. The mechanism is explored in the article from immobilization-induced skeletal muscle atrophy, the dropping of muscle power, reduction of vessels density in muscle and metabolic disturbance of muscle. The authors of the article advances that shortening the period of immobilization as possible and performing early rehabilitation are important to remove the negative influence of immobilization, but all treatments should obey the medical rules.

Acid/base mutants of glycosidases, namely thioglycoligases, are able to catalyze thioglycosides synthesis. Now, many thioglycoligases, including ?-thioglucoligase, ?-thiomannoligase, ?-thiogalactoligase, ?-thioxyloligase and ?-thioglucoligase, have been developed from bacteria and archaebacteria, and applied in synthesizing various thioglycoligases. Recently, thioglycoligases have been used to glycosylate the glycoprotein and firstly generate the thioglycoprotein. The novel extended synthetic function of glycosidases would promote the development of glycobiology, biotechnology and pharmacy.

OBJECTIVE: To establish miniSTR fluorescent detection system with all detected fragments below 150 bp and to enhance the efficiency of detecting the degraded DNA samples. METHODS: All candidate primers were designed by Primer Premier 5 and screened by FastPCR 6.0. The miniSTR multiplex system was established by these selected loci labeling by four fluorescent dye. The parameters of PCR and primer concentrations were subsequently optimized. The electrophoresis was fulfilled under POP4 on 3100-Avant and the typing data was validated by standard DNA 9947A and 007. Fresh blood samples and difficult degraded DNA samples were tested to evaluate the usefulness of the system. RESULTS: All amplicons in the established miniSTR fluorescent detection system (D12ATA63, D2S1776, D1GATA113, D4S2408, D17S974, D20S482, D3S3053, Amelogenin, D6S474, D9S1122) were less than 150bp. The profile showed a balanced peak height without extra stutter by optimal protocol. Allele frequencies showed no deviations from Hardy-Weinberg equilibrium. The system showed accumulated probability of discrimination 0.999 999 983 and accumulated triplet excluding probability of paternity 0.996 8. It could detect corrupt muscle tissue, low copy number DNA samples and human tissues fixed by 40% formaldehyde solution for 12 days. CONCLUSION The miniSTR fluorescent detection system could be solely used for personal identification of degraded DNA samples or complementally used for paternity tests. And the system could enhance the ability of detecting the trace and degraded DNA.
DNA/chemistry* ; DNA Fingerprinting ; DNA Primers/genetics* ; Electrophoresis, Agar Gel ; Forensic Genetics ; Gene Frequency/genetics* ; Genetic Markers/genetics* ; Genetics, Population ; Humans ; Polymerase Chain Reaction/methods* ; Reference Standards ; Sequence Analysis, DNA/methods*

A highly phytase-producing strain B.licheniformis LL8 was obtained by several mutagenesis of UV with B.licheniformis as starting strain.The new strain produced about two folds of phytase activity as compared with the starting strain. The production performance of the strain was stable. The cultivation conditions were optimized by single factor and orthogonal experiment. When the mutant B.licheniformis LL8 was cultivated at 55℃, initial pH 7.5 with the inoculation size of 10% for 30h in WBE medUm, the phytase activity was up to 2268.4U/mL.

Objective To identify knowledge structure and knowledge requirement of undergradu-ate course nursing teachers in our province, and offer corresponding training suggestions to construct teaching staff with reasonable knowledge structure. Methods The author adopted investigation method by giving questionnaire to 181 nursing teachers that assumed undergraduate course nursing education, the main content included general circumstance, knowledge structure and knowledge requirement of un-dergraduate course nursing teachers in Heilongjiang province. Results Nursing teachers in the fields of knowledge all had low score items, they most lacked foreign languages and expertise knowledge, the ur-gent need was to improve teaching theory and practice of teaching ability. Conclusions Nursing teach-ers possess some weak links in all areas of knowledge to different' extent, and the knowledge structure needs to be further improved. The college leaders should provide targeted training combined with nursing teachers' knowledge needs.

Objective To understand the basic structure of undergraduate nursing education teachers in Heilongjiang province in order to provide the scientific reference for the construction of our provincal nurse teachers troop.Methods Using self-made questionnaires and in the form of mail,the investigation about the basic structure and existing problems of undergraduate nursing education teachers in Heilongjiang province was carried out from December 2006 to March 2007.Results The finding was that at present some disparities existed between the basic situation of the teachers troop and higher nursing education development request.Number of professional and part-time teachers bad very wide difference;the professional title distribution was unreasonable in various schools and the proportion of double-teacher type was somewhat low,education background levels were different and the teachers who had hish education qualification were scarce;the teachers'insufficient teaching experience caused the poor teaching basic skills and so on.Conclusions There exists certain problems in undergraduate nursing education teachers in Heilongjiang province.Not until the positive countermeasures according to the cause were taken,can we construct a nursing teachers troop which has the required education background,the reasonable structure and the developmental potential.