1.Two cases of rare abnormality karyotype of chromosome in one family.
Chinese Journal of Pediatrics 2008;46(3):235-236
Adult
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Chromosome Aberrations
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Female
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Humans
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Infant, Newborn
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Karyotyping
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Male
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Pedigree
2.Expert's Comment.
Chinese Journal of Pediatrics 2011;49(8):616-617
Female
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Humans
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Male
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Sepsis
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complications
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Wernicke Encephalopathy
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diagnosis
3.The kinds of oral rehydration salts solution and its application in treating acute diarrhea of children
International Journal of Pediatrics 2010;37(1):62-64
Since the oral rehydration salts solution(ORS) has been used to treat acute diarrhea of children, there are different kinds of ORS in order to satisfy the need of clinical work. They are the initial WHO-ORS, other substances-supplemented ORS and the reduced osmolarity ORS, each of which has their own efficiency and advantage.
4.Warm needling at Jiaji (EX-B 2) for 62 cases of coronary heart disease.
Li GUO ; Yong-chun LI ; Dong-mei SUN
Chinese Acupuncture & Moxibustion 2014;34(9):861-862
Acupuncture Points
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Acupuncture Therapy
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instrumentation
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methods
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Adult
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Coronary Disease
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therapy
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Female
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Humans
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Male
;
Middle Aged
6.Effect of diameter-controlled Ti-TiO2 nanotubes on the adhesion of osteoblast and fibroblast.
Hong-cai LI ; Yu-mei ZHANG ; Hai-ping SUN
Chinese Journal of Stomatology 2012;47(2):122-126
OBJECTIVETo study the effect of Ti-TiO2 nanotubes with different diameters on the adhesion of fibroblast and osteoblast, and to find which diameter was more favorable for cells' respective adhesion.
METHODSPure titanium sheets were polished and then anodized at different potentials for 1 h with Ti as anode and Pt as cathode. TiO2 nanotubes formed at 1, 5, 10 and 20 V potentials served as experimental groups and polished pure titanium served as control group. Field emission scanning electron microscopy (Fe-SEM) was used to analyze the surface topography. Stained nucleus with Hoechst33342 were used to measure the cell adhesion. The cell shape on the sample surface were analyzed with Fe-SEM.
RESULTSTiO2 nanotube array of different inner diameters from 15 nm to 100 nm were grown on titanium sheets by anodization at potentials from 1 to 20 V. At 30, 60 and 120 min, fibroblast adhesion at nanotubes anodized at 5 V was (141 ± 9), (388 ± 14) and (489 ± 15) respectively, significantly less than any other nanotube surface at the same time (P < 0.01). Nanotubes anodized at 20 V had the least inhibitory effect for fibroblast adhesion with a number of (579 ± 14) at 120 min, and the cell shape was also inhibited. At 30, 60 and 120 min, osteoblast had a significant better adhesion on nanotubes formed at 5 V than it did on any other surface at the same time (P < 0.01), except the control group at 30 min, with the adhesion number of (198 ± 10), (431 ± 10) and (501 ± 10) respectively, and osteoblast had a abundant spread on nanotubes formed at 5 V; while osteoblast adhesion on nanotubes anodized at 20 V was (152 ± 11), (403 ± 9) and (465 ± 12) respectively, less than on any other nanotube surface within the same time (P < 0.05), and the cell shape on the surface changed to be more elongate.
CONCLUSIONSFibroblast adhesion is inhabited more or less on Ti-TiO2 nanotubes of different diameters. Nanotubes formed at 5 V have the most osteoblast adhesion, and inhibit fibroblast adhesion.
Animals ; Cell Adhesion ; Fibroblasts ; cytology ; ultrastructure ; Mice ; Microscopy, Electron, Scanning ; Nanotubes ; chemistry ; Osteoblasts ; cytology ; ultrastructure ; Surface Properties ; Titanium ; chemistry
7.Gastrointestinal and pancreatic neuroendocrine tumors: a clinicopathologic and immunohistochemical study.
Li-mei SUN ; Xue-shan QIU ; En-hua WANG
Chinese Journal of Pathology 2012;41(10):696-697
Adolescent
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Adult
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Aged
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Chromogranin A
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metabolism
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Female
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Gastrointestinal Neoplasms
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metabolism
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pathology
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Humans
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Immunohistochemistry
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Ki-67 Antigen
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metabolism
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Liver Neoplasms
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metabolism
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pathology
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Male
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Middle Aged
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Neoplasm Staging
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Neuroendocrine Tumors
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metabolism
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pathology
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Pancreatic Neoplasms
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metabolism
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pathology
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Synaptophysin
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metabolism
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Young Adult
8.Factors affecting the success rate of artificial insemination with donor sperm.
Xiu-fang LI ; Hua-rui FAN ; Yan SHENG ; Mei SUN
National Journal of Andrology 2015;21(3):234-238
OBJECTIVETo explore various factors affecting the clinical pregnancy outcomes of artificial insemination with donor sperm (AID).
METHODSWe retrospectively analyzed 15,744 cycles of AID in 6302 women and investigated the association of the clinical pregnancy outcomes of AID with the treatment protocols, the times of insemination per cycle, the age of the infertile women, the status of the oviduct, and the number of AID cycles.
RESULTSThe pregnancy rate of AID was higher in the chlomiphene-treated women than in those of the natural cycle group (P = 0.003) but showed no significant differences either between the chloramiphene and human menopause gonadotropin (HMG) or between the HMG and natural cycle groups (P > 0.05), and so was it in the women that had received AID twice per cycle before and after ovulation (26.3%) than in those that had undergone only once before (7.0%) or after ovulation (23.7%) (P < 0.05). However, the pregnancy rate was remarkably lower in the women aged 35-40 years (16.5%), especially in those over 40 years (1.2%), than in those under 35 years (26.0%) (P < 0.05). There was no significant difference in the success rate of AID between the women with oviductal adhesion and those without (27.4% vs. 28.1%, P > 0.05). The pregnancy rate of the first cycle of AID (27.6%) was markedly higher than those of the second (24.7%), third (23.9%), and fourth (23.1%) (P < 0.01), but with no significant differences among the latter three cycles (P > 0.05), while that of the fifth cycle (19.0%) was remarkably lower than those of the first four (P < 0.01).
CONCLUSIONThe age of the infertile women is an important factor affecting the success rate of AID. AID twice per cycle is better than once only. For those without oviductal factors, at least 4 cycles of AID are required before in vitro fertilization.
Adult ; Age Factors ; Female ; Fertilization in Vitro ; Humans ; Infertility, Female ; Insemination, Artificial ; Insemination, Artificial, Heterologous ; Ovulation ; Ovulation Induction ; Pregnancy ; Pregnancy Outcome ; Pregnancy Rate ; Retrospective Studies
9.Fluorescence enhancement of flavoxate hydrochloride in alkali solution and its application in pharmaceutical analysis.
Wen-hong LI ; Chong-mei SUN ; Yong-ju WEI
Acta Pharmaceutica Sinica 2015;50(10):1324-1329
Fluorescence enhancement reaction of flavoxate hydrochloride (FX) in strong alkali solution was studied, the mechanism of the reaction was investigated, and a novel fluorimetric method for analysis of FX in drug sample was established. FX has no intrinsic fluorescence, but it can slowly produce fluorescence in strong alkali solution. Heating can promote the fluorescence enhancement reaction. In 3D fluorescence spectra of the decomposition product of FX, two fluorescence peaks, located respectively at excitation wavelengths λex/ emission wavelength λem =223/410 nm, and 302/410 nm, were observed. Using quinine sulfate as a reference, fluorescence quantum yield of the decomposition product was measured to be 0.50. The structural characteriza- tion and spectral analysis of the decomposition product reveal that ester bond hydrolysis reaction of FX is firstly occurred during heating process, forming 3-methylflavone-8-carboxylic acid (MFA), then a cleavage reaction of the γ-pyrone ring of MFA occurred, producing α, β-unsaturated ketone. This product includes adjacent hydroxyl benzoic acid group in its molecule, which can form intramolecular hydrogen bond under alkaline condition, so that increase the conjugate degree and enhance the rigidity of the molecule, and thereby cause fluorescence enhancement. Based on this fluorescence enhancement reaction, a fluorimetric method was proposed for the determination of FX. A linear calibration curve covered the concentration range 0.020 3-0.487 µg · mL. The regression equation was I(F) = 23.9 + 5357.3 c, with correlation coefficient r = 0.999 7 (n = 8), detection limit D = 1.1 ng · mL(-1). The method was applied to the analysis of FX tablets, with a spiked recovery rate of 100.2%. The reliability of the method was verified by a UV-spectrophotometric method.
Alkalies
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Calibration
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Chemistry, Pharmaceutical
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Flavoxate
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analogs & derivatives
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chemistry
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Fluorescence
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Limit of Detection
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Reproducibility of Results
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Solutions
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Tablets
10.Prenatal diagnosis of Bart's fetus using real time fluorescent polymerase chain reaction.
Mei YAN ; Nian-gu SUN ; Li-rong WANG
Chinese Journal of Pediatrics 2007;45(9):714-715
DNA
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analysis
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Female
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Humans
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Neonatology
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Polymerase Chain Reaction
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Pregnancy
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Prenatal Diagnosis
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methods
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alpha-Thalassemia
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diagnosis
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genetics