Objective To explore the relationship between plasma homocysteine(Hcy),folic acid,VB12 level and deep venous thrombosis(DVT).Method With case-control study design,fifty DVT patients were included in this study as case group,and 50 healthy subjects selected from the same area as control group.Epidemiological survey were made,and 4ml fasting venous blood was collected for all subjects.Radio-immunoassay and fluorescence polarization immunoassay(FPIA) were used to measure blood serum folic acid,VB12 and plasma Hcy levels respectively.The correlation between Hcy level and DVT was analyzed.Results Plasma Hcy level of subjects in case group was higher than that in control group,There was significant difference of HHcy and low serum folic acid between case and control,and the OR(95%CI) was 3.74(1.21-11.59)and 0.17(0.05-0.53) respectively.There existed correlation among Hcy level,folic acid and VB12 levels.Partial correlation analysis showed the same results between Hcy level and folic acid level,VB12 level and folic acid level,while there existed negative correlation between Hcy level and VB12 level.Conclusion HHcy and low blood folic acid level increased DVT risk.Low blood levels of folic acid and VB12 increase the risk of HHcy.

Objective To study the effect of sub-clinical dosage of EPO on early renal lesions of diabetic rats. Methods The rats were randomly divided into normal control group (NC), diabetic group (DM), diabetic rats treated with A-dosage EPO (DMTA), and diabetic rats treated with B-dosage EPO (DMTB). Diabetic rats induced by STZ were given EPO intraperitoneally for 10 weeks; then blood glucose, blood pressure, renal function indexes, renal morphological parameter, the expression of cell apoptosis associated protein Bcl-2/Bax and VEGF were measured. Results After treatment of EPO, renal functional and morphological indexes were improved significantly and the ratio of Bcl-2/Bax and VEGF increased, both in a dose-dependent manner. Blood sugar, blood pressure and hemoglobin concentration had no significant differences. Conclusion Sub-clinical dosage of EPO could play an advantageous role in diabetic nephropathy. Inhibition of cell apoptosis by upregulation of Bcl-2/Bax expression rate may be responsible for renal protective effects despite the upregulation of VEGF.

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Indoles ; therapeutic use ; Liver Neoplasms ; drug therapy ; Male ; Middle Aged ; Pyrroles ; therapeutic use

[Abstract ]Objective:To estimate dental maturation norms of permanent dentition in a group of Nanjing children using Nolla's tech-nique.Methods:549 cases of panoramic radiographs (279 males and 270 females)of Nanjing children at the age range of 3 to 16 years were collected for the study.All permanent mandibular teeth on the left side (except for the third molars)were scored according to Nolla's method,and data were presented in tables and used to plot dental maturation curves for the Nanjing boys and girls.Results:Females were found to be more advanced in the degrees of dental development.Dental maturation is significantly associated with chron-ological age (for males and females:r =0.959,P <0.001 and r =0.953,P <0.001,respectively).The mathematical model of them was established,and then the fitting equation was obtained.Conclusion:The dental maturation curve and chronology of permanent dentition can provide the growth norms of permanent teeth for Nanjing children and facilitate the way for dentists to assess the growing children during diagnosis and treatment planning.

Objective To analyze the present situation about early screening of developmental dysplasia of the hip( DDH) of infants in Shapingba district of Chongqing,and to explore the methods to improve the rate of diagnosis about DDH. Methods A total of 2 066 infants among 0~6 months old living in Shapingba district of Chongqing,were examined with clinical and ultrasound method on hip. Results Six infants with DDH were discovered among the 2 066 infants,which was more common in the female baby. The deficiency of hospital in which can exam hip with ultrasound method and the deficiency about DDH were the significant factors affecting the low detection rate. Conclusion Necessary measure must be taken to improve the low detection rate of DDH.

Objective To study the effect of hepatectomy operation on circulating tumor cells of liver cancer patients during surgery. Methods Circulating blood was draw from 18 liver cancer patients before and after hepatectomy and 20 healthy volunteers. Blood routine ex-amination for leukocyte enumeration and karyote counting by microscope were taken for every blood samples to compare with the number of leukocytes and karyotes between pre-hepatectomy and post-hepatectomy blood of liver cancer patients. Immunofluorescence and cell culture techniques were used to identify the tumor cells and compare with the numbers and characteristics of the circulating tumor cells in blood sam-ples among all groups. Fluorescent antibodies,AE1/AE3 and CD133 were used to mark tumor cells. Results The number of leukocytes and karyotes in post-hepatectomy blood samples of liver cancer patients increased compared with pre-hepatectomy blood samples. The AE1/AE3 positive cells-samples were more than CD133 positive cells-samples and double positive cells-samples(P<0. 001). AE1/AE3 positive cells of healthy volunteers’ blood were more than post-hepatectomy blood of liver cancer patients(P<0. 05). No significant differences in CD133 positive cells and double positive cells were found among all groups(P>0. 05). There were no significant differences in clone formation rate after cell culture among all groups(P>0. 05). For 3 clone formed samples of liver cancer patients,there were no differences in tumor source, pathologic type and differentiation level. Conclusion The circulating blood of liver cancer patients,non-cancer patients and healthy people all exist circulating tumor cells. Hepatoma resection does not affect the number and characteristics of circulating tumor cells in liver cancer pa-tients’ blood.

Objective To investigate the clinical features and the findings of coronary angiography,the treatment and prognosis of patients with myocardial bridge.To increase our knowledge on myocardial bridge.Methods Fifty two patients were diagnosed as myocardial bridge by coronary angiography in our hospital from January 2001 to December 2004. Angiographically,systolic compression of the arterial lumen that disappears during diastole was considered diagnostic of a myocardial bridge.Analyse the clinical features and therapy condition.Follow patients by telephone or clinical visits.Results Our study included fifty two patients.Male patients were more than female ones and the average age was 53?12 years old.Myocardial bridge was the most common in the middle segment of the left anterior descending artery.Majority of the patients took medication,and 2 of them were treated with intracoronary stent implantation.Forty patients were followed.During a mean 1.9?1.1 years follow-up period,there was no cardiac death.25 of the patients required medication,and 1 of 2 patients who underwent stent implantation had in-stent restenosis at 3.3 years.Conclusion Patients with myocardial bridge may present with atypical chest pain.Major patients with myocardial bridge are treated with medication,and stent implants may improve patients' symptoms.The prognosis of the patients with myocardial bridge is usually good.

AIM: To investigate the effect of Ginsenoside Rb1(G-Rb1) on transcription of glucocorticoid receptor ? (GR?) mRNA and expression of its protein in a acute promyelocytic leukemia cell line. METHODS: Antiproliferation effects of G-Rb1 on HL60 cells were determined by MTT. The transcription of GR mRNA and expression of GR were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively. RESULTS: G-Rb1 inhibited the growth of HL-60 cell line in a time-dependent and dose-dependent manner. After 36 hours treatment with 80 mg/L G-Rb1, the expression of GR? mRNA and GR protein increased. CONCLUSION: G-Rb1 exhibits the inhibitory effects on cell growth in HL60 cells, and increases the expression of GR? mRNA and protein. [

Objective To explore the effect of deep vein thrombosis(DVT)assessment scale on prevention of deep vein thrombosis in malignant tumor patients?Methods Eight hundred malignant tumor patients were divided into intervention group and control group according to admission time,400 cases in each group?The control group received traditional nursing.Methods of treatment and nursing,while the intervention group received pertinent nursing care based on the risk factors for DVT induced for the investigation among the patients using deep vein thrombus assessment scale.Result The incidence of DVT in the intervention group was significantly lower than that of the control group(P<0?05).Conclusions The deep vein thrombosis assessment scale may effectively predict the population at high risk of deep vein thrombosis?The implementation of effective nursing intervention may reduce the incidence of DVT?

According to the method of puncturing the twelve Jing (well) acupoints to treat hemiplegia followYinbai (SP 1), Dadun (LR 1), Shangyang (LI 1) and Guanchong (TE 1), combining with selected acupoints according to the presenting syndromes, to treat 114 cases of hemiplegia following a stroke, the total effective rate was 91.1%.