AIM: To investigate the extraction of anti-thrombotic contituent from Earthworm ( Pheretima). METHODS: The prothrombin time (PT)of rabbit blood and antithrombin of 14 samples that were extracted by different methods were determined in vitro. RESULTS: The sample that was percolated with 70% alcohol and the sample that was extracted by semi-bionic method were better than ones that were extracted by other methods. CONCLUSION: It is a simple and practicable method to percolate Earthworm with 70% alcohol.

Objective To explore the expression of PTEN,NF-KB p65 and Survivin protein and the influence in the genesis and development of endometrial carcinoma(EC).Methods The expression of PTEN.NF-KB and Survivin in 63 cases of EC and 20 cases normal endometrial tissue specimens were detected by immunohistochemistry.Resuits There were obvious differences among the positive rates of PTEN.N-KB p65 and Survivin protein in EC compared with in normal endometrial tissue specimens (P＜0.001).The clinicopathological characteristics of endometrial carcinoma with PTEN,NF-KB p65 and Survivin were as follows:the expression of PTEN gene was positively correlated with the degree of histological differentiation(P＜0.001),and it was negatively correlated with lymph node metastasis and TNM (P＜0.005).There was inverse correlation between the expression of NF-KB p65 gene and the degree of histological differentiation(P＜0.05).It was positively correlated with lymph node metastasis,deep myometrial invasion and TNM in EC (P＜0.05).The expression of Survivin was positively correlated with the clinical stage and lymph node metastasis and deep myometrial invasion in EC(P＜0.05).Conclusion There are difierent extent action of PTEN.NF-KB p65 and Survivin significantly in genesis and development of EC.Detection of PrEN combined with NF-KB p65 and Survivin is valuable for early diagnosing and evaluating malignancy extent of EC.

Objective To explore a safe and effective imaging method by the application of 3D contrast-enhanced MR Angiography (3D CE-MRA) with low-or conventional-dose contrast agent to the examination of lower extremity arterial disease.Methods Totally 31 patients suspected with lower extremity arterial stenosis or occlusion underwent 3D CE-MRA examination including one patient had right leg cut due to the disease,of whom,18 ones had conventional-dose contrast agent (38 ml) injected in 1.5T MR machine and 13 ones had low-dose agent (15 ml) injected in 3.0T MR machine.Quality evaluation was carried out for the images by different doses of agents.Results All the patients had no agent allergy occurred,and one patient had left tibia-fibula motion artifact due to involuntary movement.3.0T MR machine with low-dose agent behaved better than 1.ST MR machine with conventional-dose agent in leg imaging,while there were no significant differences between the quality of the images except that of popliteal artery (P＞0.05).Conclusion 3D CE-MRA is an effective and safe method in detecting lower extremity artery disease,and 3.0T MR machine with low-dose agent lays a foundation for clinical diagnosis and treatment.

Objective To investigate health behavior of senile patients with hypertension and influencing factors.Methods Using general data scale,health conception scale,self-rated abilities for health practice scale and health-promoting lifestyle profile,206 patients with hypertension in the clinic and inpatient department were selected by convenience sampling method to investigate their health behavior.Results The score of health behavior of senile patients with hypertension was (72.25±16.66),23.8％ of patients were at high level.Among the subscale of health-promoting lifestyle profile,the score of nutrition was the highest,activity and healthy responsibility were the lowest.The score of health promotion was positively correlated with health conception,self-efficacy and education level,while negatively correlated with unhealthy habits.Conclusions Education about activity and healthy responsibility should be enhanced,we should set up right health conception,develop effective measures to enhance self-efficacy in order to improve health behavior of older patients with hypertension.

Object To establish cell suspension culture system in leaves of seedling of Pueraria lobata (Willd.) Ohwi Methods The callus induced from leaf explants of P.lobata seedling were suspended in liquid medium. The time courses of cell growth and yields of puerarin and isoflavones were studied Results The log phase of the cell cultures' growth was from 1st d through 5th d. The dry weight accumulation reached highest on 8th d. The production of puerarin was highly coupled with the cell growth. The yield of puerarin reached highest on 8th d. However, the accumulation of total isoflavones was different from that of puerarin. The yield of total isoflaones reached the highest on 10th d Conclusion The yield of total isoflavones in cell suspension culture system is higher than that in wild plants, while the yield of puerarin is lower than that in wild plants

Telomere is the end structure of chromosome and it will be shortened during replication． Telomerase is a reverse transcripatse consisting of both RNA and protein components and synthesizes telomeric DNA by copying the template sequence of its own RNA components to maintain telomere length for function．Telomerase activity in germline cells，immortal and neoplastic cells was detected，but not in mostly normal cells．The telomere-telomerase hypothesis was brung out to explain this phenomenon．According to this hypothesis，re-actived telomerase will maintain telomere's length for protecting chromosome to make the cell immortal．The aging procedure will be explained by this hypothesis too．This hypothesis provides a new concepts of cancer and cancer's treatment．

Objective:To study the effects of salidroside (Sal) on autophagy of pancreatic cells in rats with severe acute pancreatitis (SAP).Methods:Fifty healthy male Wistar rats were randomly (random number) divided into the sham group, SAP group, Sal 15 group, Sal 30 group and Sal 60 group, 10 rats in each group. The sham group was injected retrogradely normal saline into pancreatic duct, while the 5% sodium taurocholate was injected retrogradely into pancreatic duct to establish severe pancreatitis model in the SAP group and all the Sal groups. Salidroside at different doses were intraperitoneally injected in all the Sal groups at 2 h after surgery. The serum amylase (AMY) activity and plasma tumour necrosis factor-α (TNF-α) level were detected by enzyme-linked immunosorbent assay (ELISA), and Western blot was used to detect the expression of pancreatic tissue protein. The histopathological changes of pancreas were observed under the light microscope. The data were analyzed by SPSS 24.0, and One-way ANOVA was used for comparison between groups. A P value less than 0.05 was considered to be statistically significant. Results:After salidroside intervention, the levels of TNF-α (28.1±0.9) pg/mL, the relative expression of p65 (0.52±0.01), AMY activity (3 801±292) and relative expression of p-mTOR were the lowest in the Sal 60 group (all P<0.05); while the relative expression of LC3 was the highest in the Sal 60 group (all P <0.05). The degree of pathological damage of pancreatic tissues was decreased with the increase of salidroside dose. Conclusions:Salidroside at different doses have protective effects on rats with severe pancreatitis, which seems to be most obvious in the Sal 60 group.

Child ; Humans ; Pulmonary Embolism ; diagnosis ; etiology ; therapy ; Risk Factors

OBJECTIVE:To investigate therapeutic efficacy of bifidobacteria triple viable bacteria combined with infliximab in the treatment of moderate and severe ulcerative colitis(UC),and its effects on immune function. METHODS:Seventy-two UC pa-tients were selected from gastroenterology department of the First Affiliated Hospital of Chongqing Medical University during Jun. 2009 to Jan. 2015. They was divided into single group and combination group according to therapy plan,with 36 cases in each group. Single group was given infliximab 5 mg/kg,ivgtt,on 0,2nd,6th,8th week. Combination group was additionally given bi-fidobacteria triple viable bacteria 420 mg,tid. Both groups were treated for consercutive 2 months. Clinical efficacies were com-pared between 2 groups as well as the levels of humoral immunity and T cell function. The occurrence of ADR was observed in 2 groups. RESULTS:Clinical response rate of combination group was 86.11%,which was significantly higher than 52.78% of single group,with statistical significance (P<0.05). Before treatment,there was no statistical significance in humoral immunity and T cell function between 2 groups(P>0.05). After treatment,the levels of CD4+ and CD4+/CD8+ were significantly increased in both 2 groups,while the levels of CD8+,Th1 and Th2 were significantly decreased;the combination group was significantly better than the control group,with statistical significance (P<0.05);there was no statistical significance in CD3+ between 2 groups before and after treatment(P>0.05). After treatment,the levels of IgA and IgG were increased significantly in 2 groups,and the level of C3 was decreased significantly;the combination group was significantly better than the control group,with statistical significance (P<0.05);there was no statistical significance in IgM content and C4 level between 2 groups before and after treatment (P>0.05). There was no statistical significance in the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:Bifidobacteria triple viable bacteria combined with infliximab shows significant therapeutic efficacy in the treatment of moderate and severe UC, and can significantly improve humoral immunity function and the recovery of T cell level,with good safety.

Objective To investigate the clinical curative effect of closed thoracic drainage by minimal invasive with needle catheter in treating spontaneous pneumothorax.Methods Seventy-six patients with spontaneous pneumothorax were divided into two groups randomly,the control group was treated by traditional thick tube closed thoracic drainage,the experimental group was treated by the needle catheter minimally invasive thoracic closed drainage.Compared clinical curative effect,pulmonary reexpansion time,the wound healing time,the degree of pain and the occurrence of complication between two groups.Results The effective rate of experimental group and control group was 91.9％ (34/37) and 92.3％ (36/39),the pulmonary reexpansion time of experimental group and control group was (5.38 ± 2.21) d and (5.29 ± 2.27) d,there was no significant difference (P ＞ 0.05).The pain scores during draining and wound thealing time of experimental group and control group was (1.32 ±0.92) scores,(2.93 ± 1.14) scores,(2.13 ± 1.40) d and (5.32 ± 1.78) d,there was significant difference (P ＜ 0.01).Complication such as subcutaneous emphysema,dislodgment of the catheter and wound infection in experimental group was lower than that in control group.Conclusions The effect of needle catheter minimally invasive thoracic closed drainage in spontaneous pneumothorax is better,simple and less invasive.The wound healing time is short,and little pain.It is worthy of promotion.