Purpose:To investigate the rational choice of adjuvant treatment and its clinical value for stage Ⅰb, Ⅱa cervical cancinoma after radical hysterectomy and pelvic lymphadenectomy.Methods:126 patients with stage Ⅰb and Ⅱa cervical cancer received adjuvant radiotherapy and/or chemotherapy after radical hysterectomy and pelvic lymphadenectomy .Of these patients, 64 received radiotherapy only, 26 chemotherapy only, 36 had both. The prognostic factors and treatment results were analyzed.Results:The overall 5-year survival rate for the 126 patients was 73%(92/126). For the radiotherapy only, chemotherapy only, and the radio-chemotherapy groups, the 5-year survival rates were 76.6%(49/64), 69.2%(18/26), and 69.4%(25/36) respectively. The clinical stages and the number of pelvic lymphnodes metastases were the most important prognostic factors. Conclusions:The value of adjuvant treatment for early stage cervical cancer after radical surgery was limited, but for patients who had more than two high risk factors, adjuvant treatment should be given, and at the same time, we should emphasis the importance of thoroughness of radical hysterectomy and pelvic lymphadenectomy.

Arsenic ; blood ; urine ; Environmental Monitoring ; methods ; Humans ; Spectrometry, Fluorescence ; Spectrophotometry, Atomic

It is necessary to determine the position of break points in order to study the structure rearrangement of chromosomes and gene mapping. Therefore it is indispensable to set up region, banded idiogram and nomenclature system. Nesbitt and Franke reported an idiogram and a nomenclature system for band patterns of mouse chromosomes which thereafter was accepted by the Committee on Standardized Genetic Nomenclture. Nevertheless, there are few data of systematical contrast studies in this domain. There are still difficulties for distinguishing between some chromosomes because they are similar and variability. In this paper, we compared our G-banding karyotypes with the banding patterns of Nesbitt's idiogram. Our 400 karyotypes come from bone marrow cells and fibroblasts of several normal inbred and outbred mice. Each banding obtained from each well banded chromosomes of different cells were contrasted to that of Nesbitt's idiogram. The results are as follows: 1. we have not found so many bands in one metaphase chromosome banded with trypsin-Giemsa technique as reported by Nesbitt. 2. The banding numbers and positions on each well banding chromosome picked out from different metaphase plates were consistent with those of Nesbitt's idiogrom. But some bands such as 1E1 and 1E2; 2E5-2F5; 4A3-4A5; 5C1-5C3 can't usually be distinguished in our data. 3. We observed that the major banding structure of each karyotype remains stability, but the minor banding structure appears to be variablitity in inbred mice. In addition, 6 variant pictures and region idiograms in the G-banding structure of each chromosome were showed and some notices for correct analysis of mouse karyotype were discussed.

Objective To prepare 60 mer oligo microarray chips for detecting human immunodeficiency virus (HIV) and for the clinical application in the detection of AIDS patient. Methods Oligonucleotide probes were designed according to the sequence information of two types of HIV. Oligo microarray was prepared by using Cartesian Microarrayer. Products of the restrictive display PCR were labeled with Cy3. Furthermore, the PCR products were sequenced. Results Using the oligo microarray, HIV infection could be detected in laboratory as well as in clinical assays. Results of hybridization indicated that 1 AIDS patient was positive and 20 health people were negative. The results obtained by sequencing confirmed the results obtained by oligo microarray studies. Conclusion The HIV 60 mer oligo microarray could be used in detecting patient HIV infection and analyzing its genotypes.

Objective To investigate the effects and the possible mechanisms of nerve growth factor (NGF) on serum levels of vascular endothelial growth factor (VEGF) and stromal cell-derived factor 1 (SDF-1) protein expression in rats with focal cerebral ischemia.Methods Male Sprague-Dawley rats weighting 200 ～ 250 g were subjected to middle cerebral occlusion (MCAO).MCAO rats were randomly divided into NGF group,saline control group and NGF+PI3K antagonist group (n =12).The neurological function was evaluated on the 4th,7th day after MCAO,and the serum levels of VEGF and SDF-1 protein were measured by ELISA.Results The neurological function was better in rats of the NGF group than those of the saline control group and NGF+PI3K antagonist on the 4th,7th day after MCAO (P ＜ 0.05).The serum levels of VEGF and SDF-1 protein of the NGF group was significantly higher than that of the saline control group and NGF+PI3K antagonist group on the 4th day after MCAO (P ＜ 0.05).Conclusions Treatment with NGF may improve neurological function of rats with focal cerebral ischemia,and upregulate serum levels of VEGF and SDF-1 protein expression.PI3K/AKT signal pathway may have attended the above regulation.

Objective To establish the crystal violet plaque assay for detection of virus titer of recombined Tiantan vaccinia AIDS vaccine,and provide more stable method of virus titration for rTV AIDS Vaccine.Methods Optimized the concentration of Vero cells,the time and temperature of virus adsorption,and the time of determination for CPE,then established the crystal violet plaque assay for virus titer of rTV.Counting and analysis the plaques by BioSpot Reader,then analyzed the relativity of plaques counted with BioSpot Reader and manual; Several lots rTV AIDS Vaccine and Tiantan vaccinia were titrated by the method of plaque formation-hemadsorption assay,neutral red and crystal violet plaque assay,then analyzed the relativity of the results of three methods ; meanwhile,the virus titer of samples were determine repeatedly by the crystal violet plaque assay,then calculated the coefficient of variation( CV),and verified the precision of the method; SPSS17.0 was used in statistical analysis of the experimental results.Results When the concentration of Vero cells was 5.0×105-9.O×105 cells/ml,virus been adsorbesd 2 h at 37℃,then cultivated 72 h after adding the culture medium containing methyl cellulose.Plaques counted by BioSpot Reader was highly related with counted by manual (r =0.985),so BioSpot Reader counting can objectively reflect the virus plaques with various size,and reduce the error by manual counting; compared the virus titration for different lots of rTV AIDS vaccine and Tiantan vaccinia with three methods,the crystal violet plaque assay was highly related with plaque formation-hemadsorption assay (r =0.997,P＜0.01 ) and neutral red plaque assay(r=0.980,P＜0.01 ).Conclusion Crystal violet plaque assay was established for virus titration of rTV AIDS Vaccine.

The need for an efficacious HIV/AIDS vaccine remains the highest priority of the world HIV/AIDS agenda. The generation of an efficacious HIV/AIDS vaccine proves an enormous scientific challenge. This article reviews the neutralizing antibody problem, elusive immune protection, immunogen design, pre-existing anti-vector immunity and design of phase 3 vaccine trials and the challenges and opportunities in development of HIV/AIDS vaccine are discussed.

Objective To detect the main virulence genes and biofilm formation of Enterococci strains isolated from blood samples .Methods Twenty-eight strains of Enterococcus faecalis ( E.faecalis) and 54 strains of Enterococcus faecium ( E.faecium) were collected from blood samples .Five main virulence genes (asa1, esp, hyl, cylA and gelE) were detected by multiplex PCR.Biofilm formation was investigated by using microtiter dish biofilm formation assay .Results All E.faecalis strains were positive for at least one kind of virulence genes , of which 14 strains were concurrently positive for asa1, esp, cylA and gelE.asa1, cylA and gelE were only detected in E.faecalis strains, while hyl gene only existed in E.faecium strains. Twenty-seven strains of E.faecium were esp positive, of which 12 strains were both hyl and esp positive. None of the 5 virulence genes were identified in 10 strains of E.faecium.85.7% of E.faecalis strains and 63.0%of E.faecium strains could form biofilm.Conclusion Compared with E.faecium strains, more types of virulence genes were detected in E.faecalis strains with higher positive rates .Moreover , E.faecalis strains were more likely to form biofilms than E.faecium strains.

Objective To calculate the incidence of pathology-proved bilateral well-differentiated thyroid carcinoma (BWDTC) in patients undergoing total or near total thyroidectomy.Methods Clinical data were retrospectively reviewed in 121 patients who underwent total or near total thyroidectomy.Thirteen indexes selected from both the unilateral thyroid carcinoma group and the bilateral thyroid carcinoma group were adopted to undergo a univariate analysis and Logistic regression analysis.Results BWDTC was found in 33.06％ patients after total or near total thyroidectomy.Logistic regression analysis showed that patient's age ＜ 50,bilateral nodules based on ultrasound and the diameter of the tirst nodule ＞ 2 cm were independent risk factors for BWDTC (P ＜ 0.05).Conclusions In the premise of a malignant first nodule found in the preoperative examinations,BWDTC should be highly suspected if the patient is under 50 years old,has bilateral nodules found by preoperative ultrasound and the diameter of the first malignant nodule is over 2 cm.

Objective To observe the changing levels of serum sFas before and after intravenous i mmunoglobulin (IVIG) treatment of incomplete Kawasaki disease (IKD),to explore the roles of sFas in the pathogenesis of IKD and IVIG treatment mechanism.Methods Thirty eight cases of IKD children were selected as experimental group and 20 examples of the same age of children as the control group.The IKD children were treated by IVIG in combination with aspirin (ASP) ; and blood test was performed before treatment,3 days after treatment,and 14 days after treatment,respectively.Dual-resistant sandwich enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of serum sFas,plasma Fibrinogen (PT-D),d-dimer (D-D),and c-reactive protein (CRP).Results The levels of serum sFas,PT-D,D-D,and CRP were significantly higher than the control group for IKD children before treatment[(0.55 ± 0.14)ng/L vs (0.24 ±0.04) ng/L,(552.3 ± 147.2) mg/dl vs (277.3 ±82.5)mg/dl,(649.0 ±201.6) μg/L vs (315.4 ±91.8)μg/L,and(72.2 ±28.7)mg/L vs (7.2 ±2.9)mg/L; t' =12.41,9.11,8.64,13.82;All P ＜ 0.05] ;3 days after treatment,compared with those before treatment and control group,the sFas level of IKD children at the third day after treatment was significantly decreased compared to that before treatment and control groups,respectively [(0.43 ± 0.09) ng/L vs (0.55 ± 0.14) ng/L,(0.24 ± 0.04) ng/L,F =47.624,All P ＜0.05] ;For the level of sFas at the 14th day after treatment,no statistical significance was found between IKD children and the control group[(0.24 ±0.05) ng/L vs (0.24 ±0.04) ng/L,t =0.596,P ＞ 0.05].Conclusions The abnormally increased serum sFas level before IVIG treatment suggests that dysfunction of apoptosis be involved in the pathogenesis of the IKD.Intravenous immunoglobulin treatment may be involved in the apoptosis process.