Objective To explore the role of halofuginone in immune regulation,observe the effects of halofuginone on maturation of dendritic cells and immune tolerance during homograft heart transplantation of rats,in order to provide new ways and means to treat transplantation tolerance and to explore its possible mechanism.Methods Rat bone marrow derived dendritic cells were isolated and the effects of halofuginone in LPS-treated DCs were investigated by flow cytometry;heart transplantation model of rats were established and the effect of halofuginone stimulated dendritic cells in the immune tolerance by pathological analysis and mixed lymphocyte reaction were observed.Results Flow cytometry results show that the expression of cell surface CD80 CD86,OX62 antigens were not high in control group,their expression increased significantly when stimulated by LPS for 24h,and decreased when stimulated by both Changshan ketone and LPS for 24h,and even lower than their expression levels in blank group,which showed that Changshan ketone has the ability to inhibit the maturation of dendritic cells induced by LPS.In rat heart transplantation model,dendritic cells stimulated with Changshan were injected into recipient rats in vivo,and receptor of rat transplantation rejection decreased significantly,which showed that dendritic cells stimulated with Changshan ketone may be used as a new method for cell therapy in treatment of transplant rejection.Conclusion This study systematically observes that halofuginone can inhibit the maturation of rat bone marrow-derived dendritic cells and lymphocyte proliferation for the first time in vivo and in vitro.

Objective To find out the clinical characteristics related to neonate food allergy.Methods Ten subjects were diagnosed as neonate food allergy at the Third Hospital of Peking University from January 201 3 to January 201 5.The following clinical data:the gestational age,genter,age of onset,feeding,clinical manifestations,white blood cell count,eosinophilic granulocyte count,C -reactive protein(CRP),routine stool test and occult blood test,blood spe-cific IgE of cow′s milk and clinical outcomes were investigated.Results There were 4 term infants,and 6 preterm in-fants,including 2 very -preterm subjects.The gender ratio between male and female cases was 73.The age of onset from 1 day after birth to 43 days after birth,clinical investigation of neonate food allergy in 1 0 cases,and the average time was 20.3 d,the median age was 1 8.5 d,and the SD was 1 3.38 d.Their rectified gestational ages were from 30 to 37 weeks in 6 cases at onset.Four infants received breast feeding,3 infants received formula milk feeding,and the other 3 infants received mixed feeding.At onset,the symptoms were almost involved in the digestive system.The white blood cell counts of lab test were almost normal in 1 0 subjects.And eosinophilic granulocytes were elevated in 8 subjects.CRP elevated in 1 case,and those of the other 9 subjects were normal.The cow′s milk specific IgE antibody was elevated in 1 subject,and that of the other 9 subjects was normal.Two subjects were performed opened food challenge test and the finding were positive.Conclusions Both the term infants and the preterm infants can have food allergy,but the age of onset ranged widely.Breast feeding infants can also be attacked.The symptoms of the digestive tract were the main clinical manifestations of such neonates.The open -food challenge test is beneficial to diagnose but difficult to practice in the neonates.Dietetic treatment is the only method can therapy the disease.And mostly the clinical outcomes are desirable.

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The present study is to investigate the protective actions of guggulsterone against the cytotoxicity produced by exposure to hydrogen peroxide (H2O2) in PC12 cells. It was evaluated by MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl-tetrazolium bromide] reduction assay, lactate dehydrogenase (LDH) release assay, and the release of nitric oxide (NO). ROS and Ca2+ in cells were evaluated by DCFH and Fura 2-AM, respectively. Mitochondrial membrane potential (MMP) was assessed by the retention of rhodamine 123 (Rh 123). Apoptosis and morphological alteration in PC12 cells were monitored with flow cytometry and electric microscope. Vitamin E, a potent antioxidant, was employed as a comparative agent. The results showed that preincubation of PC12 cells with guggulsterone (0.1-10 μmol·L-1) prevented cytotoxicity induced by H2O2. Extracellular accumulation of LDH, NO and intracellular accumulation of ROS, Ca2+ resulting from H2O2 were significantly reduced by guggulsterone. Incubation of cells with H2O2 caused a marked decrease in MMP, which was significantly inhibited by guggulsterone. The percentage of H2O2-induced apoptosis in PC12 cells was 24.3%, and decreased in the presence of guggulsterone (0.1-10 μmol·L-1) by .8.4%, 15 .9%, 11.8%, respectively. Guggulsterone exhibited comparable potency against oxidative stress induced by H2O2 in PC12 cells as that of vitamin E. The present findings showed that guggulsterone attenuated H2O2-induced cytotoxicity, extracellular accumulation of LDH and NO, intracellular accumulation of ROS and Ca2+, loss of MMP, and apoptosis, which may represent the cellular mechanisms for its neuroprotective action.

Objective To provide evidence for college students'sex education by analysis they status and gender identity of col-lege students'sex knowledge,attitude and behaviors in Guangdong province.Methods By investigating 928 college students of 12 colleges.Results Sex knowledge:Students'awareness of sexual knowledge is low average accuracy rate of question was 37.34%;sex attitude:for sex is a basic human need,love can be sexual,sexual fantasy dreams,masturbation,premarital sex and behavioral aspects of these ideas,the overall degree of recognition is (67.35%,35.24%,56.90%,52.26%,58.04%),boys and girls were sig-nificant differences in acceptance levels(χ2 =7.89,χ2 =48.46,χ2 =189.30,χ2 =32.44,χ2 =27.35,all P <0.01);sex knowledge:students'awareness of sexual knowledge is low;sex behaviors:college Students'heterosexual sex and homosexual sex rates were 8.08% and 1.51%,the incidence of male heterosexual sex is far higher than girls (χ2 = 52.08,P <0.01),there is no significant difference in homosexual acts(χ2 =4.82,P <0.01),condom use rate is low,often use rate was 29.03%,girls was significantly low-er than boys(χ2 = 12.08,P < 0.01 ),they have weak sense of self-protection.Conclusion there are many problems in college students'sex behaviors,attitude and knowledge,and there is need for further strengthening and guidance;we should pay attention to gender differences in education.

Objective To understand the clinical distribution of Acinetobacter baumannii and its resistance to commonly used an‐tibiotics so as to provide the theoretical basis for rational clinical use of antibacterial medicines .Methods The clinical distribution and drug resistance situation of 206 strains of clinically isolated Acinetobacter baumannii in the Anhua County People′s during 2012-2014 were analyzed .The bacterial identification was completed by the department of clinical laboratory ,the drug susceptibili‐ty test was performed by adopting the disk diffusion method ,and the detection results were judged according to CLSI 2012 criteria . Results A total of 2 803 strains of bacteria were isolated during 2012-2014 ,including 206 strains of Acinetobacter baumannii .Its constituent ratios during this period were 6 .71% ,6 .82% and 8 .19% ,showing the increasing trend year by year .The samples were mainly isolated from sputum (78 .16% ) ,followed by urine (7 .28% ) ,bronchoaleolar lavage fluid (6 .80% ) ,wound secretion (3 .40% ) and blood(2 .91% ) .The infected patients were mainly distributed in the respiratory department (40 .29% ) and neurosur‐gery department(22 .82% ) .The detection results of Acinetobacter baumannii showed the higher resistance to 20 kinds of commonly used antibacterial drugs ,in which the drugs with most significantly increase in 3‐year drug resistance rate were in turn ceftazidime , cotrimoxazole ,levofloxacin ,piperacillin and ciprofloxacin ,the 3‐year resistance rate to meropenem ,imipenem ,cefoperazone/sulbac‐tam and cefepime was less than 35% .Conclusion Acinetobacter baumannii is the major opportunistic pathogenic bacterium in noso‐comial infection with higher drug resistance rate .It is necessary to strengthen the monitoring of Acinetobacter baumannii drug re‐sistance and rationally use antibacterial medicines in order to stop the drug‐resistant strains colonization and cross expansion in hos‐pital .

Objective To identify whether soybean isoflavones plays a role in the prevention of chronicallograft nephropathy(CAN)in rats.Methods Forty-five male Lewis rats as receptors were randomly divided into 3 groups,receiving a diet with protein from high isoflavone soy protein fraction (HIS),low isoflavone soy protein fraction(LIS)or casein(CAS)starting one week before renal transplants from fisher donors,and continuing throughout the study.Serial serum and urine samples were collected at the beginning of the study,and at 4th,12th and 24th week.All rats were killed and the grafts were harvested in the 24th week.The functional and histopathogical changes of renal grafts were evaluated.Results In HIS group,arteria caudilis systolic blood pressure(SBP)value,24 h uri- nary protein(Upro)and serum creatitine(Cr)concentrations were significantly lower than those in LIS and CAS groups(all P

Objective To investigate the clinical value of the YSZ-1 uterine manipulator in total laparoscopic hysterectomy.Methods Total laparoscopic hysterectomy was employed in a total of 78 women by using the LigaSure,ultrasonic scalpel,and the YSZ-1 uterine manipulator.The anterior fornix was opened and the manipulator was placed on the lip of the cervix.Then the uterus was completely removed.Results The operation was completed smoothly in all the 78 patients.The operating time was 45~90 min(mean,63 min),and the intraoperative blood loss was 40~100 ml(mean,65 ml).No urinary tract injuries,vaginal bleeding,or other postoperative complications occurred.The length of postoperative hospital stay was 4~7 d(mean,5.3 d).Follow-up examinations in the 78 patients for 3~6 months(mean,4 months) showed that the vaginal wound normally healed up. Conclusions Use of the YSZ-1 uterine manipulator in total laparoscopic hysterectomy makes the procedure easier and safer.The YSZ-1 uterine manipulator is a useful laparoscopic instrument.

[Objective] To summarize the therapeutic and nursing care of vacuum sealing drainage to treat skin avulsion injury. [Methods]We used the method of vacuum sealing drainage combined with split thickness skin graft and pre-and post-operation nursing care to treatment of 21 patients suffering with skin avulsion injury, and compared therapeutic effect with routine therapy group. [Result]Skin graft survival rate in VSD group was higher than in routine therapy group. The dressing change, wound healing time, hospitalization time in VSD group were lower in routine therapy group. [Conclusion] The method of vacuum sealing drainage is good in treatment of intervention skin avulsion injury.

Objective To investigate the clinical significance of the combination detection of blood lactic acid,B-type brain natri-uretic peptide(BNP)and homocysteine(Hcy)in elderly acute cerebral infarction.Methods The arterial and venous blood during a-cute onset stage,at 24,48,72 h after thrombolysis and at 12 h after fasting in 85 cases of acute cerebral infarction was collected for detecting blood lactic acid,BNP and Hcy,performing the dynamic monitoring analysis and the comparative observation with the healthy control group.Results The blood lactic acid,BNP and Hcy levels in the acute onset stage group and the recovery group were significantly increased compared with the healthy control group(P <0.05).The blood lactic acid,BNP and Hcy levels during the onset stage in the acute cerebral infarction group were(6.47 ±3.92)mmol/L,(100.52 ±48.96 )pg/mL and(48.96 ±15.13 )μmol/L respectively,which were significantly higher than those at 48,72 h of postoperative recovery,the differences between them were statistically significant(P <0.05).The blood lactic acid,BNP and Hcy levels during postoperative convalescence in the cere-bral infarction group were gradually decreased and declined to the minimal level until postoperative 72 h,which were(2.16±1.83) mmol/L,(33.61±10.42)pg/mL and(18.87±8.27)μmol/L respectively.Conclusion Blood lactic acid,BNP and Hcy are closely related with the occurrence and development of cerebral infarction,the joint detection of these three indicators provide a reliable ba-sis for the early diagnosis of elderly acute cerebral infarction.The active and effective treatment on the patient should be timely a-dopted and the dynamic monitoring should be performed in order to correctly assess the prognosis.